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HUBUNGAN KADAR TARTRAZIN DAN SENG (ZN) DALAM DARAH PADA ANAK PENDERITA DEFISIT PERHATIAN DAN GANGGUAN HIPERAKTIVITAS (ADHD) Melvariani Syari Batubara; Yahwardiah Siregar; Herla Rusmarilin; Sri Soviani; Husnarika Febriani
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 4, No 1 (2017): Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v4i1.960

Abstract

This research aims to study Tartrazine and Zinc (Zn) status in blood sample of Attention Deficit Hyperactivity Disorder (ADHD) children. Deductive research using the descriptive method through 3 stage, namely : 1. Taken of blood sample, 2. Analysis of Tartrazine status in blood sample was performed by using High Performance Liquid Chromatograpy (HPLC) method, and 3. Analysis of Zinc (Zn) status in blood sample were measurements by using Atomic Adsorbance Spectrophotometry (AAS) method. Data were analyzed using descriptive analysis techniques and Chi Square tests (X2) by Computer programs (SPSS 15). The result of the assessment of Tartrazine status in blood sample of ADHD children that had been was considered statistically highly significant a increase of the assessment of Tartrazine status in blood sample of ADHD children by 130 mg/ml compared with laboratory reference (control) by 28 mg/ml, HA>C** (p<0,001). The result of the assessment of Zinc (Zn) status in blood sample of ADHD children that had been was showed statistically highly significant a reduce of the assessment of Zinc (Zn) status in blood sample of ADHD children by 0,37 µg/ml compared with laboratory reference (control) by 0,64-1,18 µg/ml, HA<C** (p<0,001). This was considered a reduction and increase in Tartrazine and Zinc (Zn) concentrations induces of the Hyperactivity children by 95%.
The Relationship between Tyr113His Epoxidized Hydrolase Enzyme Gene Polymorphisms and the Incidence of Smoking Lung Cancer Lucia Aktalina; Amira Permatasari Tarigan; Noni Novisari Soeroso; Yahwardiah Siregar; Ozar Sanuddin
Jurnal Respirologi Indonesia Vol 38, No 2 (2018)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v38i2.163

Abstract

Background: This study aims to determine the relationship between the gene polymorphism of the enzyme Epoxid Hydrolase (EPHX) Tyr113His and the tendency of smokers to suffer from lung cancer. The EPHX Tyr113His gene polymorphism is a change in one nucleotide base of the EPHX gene to produce enzymes with decreased function by 50%. EPHX plays a role in the metabolism of chemical compounds in cigarette smoke, especially Benzo (a) Pyren (BaP), which becomes a carcinogen in the body. Methods: This study is a case-control study design conducted from October 2016 to February 2017. Venous blood was taken from 35 people with lung cancer at RSUP H Adam Malik Medan and 35 healthy smokers. The genotype examination of the EPHX gene was carried out using the PCR-RFLP method in the USU FK Integrated Laboratory. Results: The genotype frequency of heterozygous variant T/C and homozygous variant C/C in case groups was 83.3% and 16.7%, respectively. The frequency of the T allele in the case group was 41.67% and the C allele was 58.33%. Whereas in the control, the frequency was heterozygous variant T/C (80%) and homozygous variant C/C (20%). The frequency of the T allele in the control group was 40% and the C allele was 60%. There were no homozygous wildtype T/T genotypes in the two groups. There was no significant difference in the genetic distribution and allele distribution of the EPHX enzyme between case and control groups. Conclusion: There is no relationship between EPHX Tyr113His enzyme gene polymorphisms and the incidence of lung cancer in smoking. J Respir Indo 2018; 38(2): 93-9)
ANALISIS KADAR VITAMIN D PADA MAHASISWA INSTITUT KESEHATAN MEDISTRA DAN MAHASISWA FAKULTAS KEDOKTERAN GIGI UNIVERSITAS SUMATERA UTARA Miftahul Zannah; Yahwardiah Siregar; Ariyati Yosi
JURNAL KEPERAWATAN DAN FISIOTERAPI (JKF) Vol 2 No 1 (2019): Jurnal Keperawatan dan Fisioterapi (JKF)
Publisher : Fakultas Keperawatan dan Fisioterapi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.993 KB) | DOI: 10.35451/jkf.v2i1.181

Abstract

Vitamin D levels influenced by many factors like formation, nutritions, malabsorbtion and degradation of 25(OH)D. Formation factors is one of important for metabolism vitamin D. Vitamin D is the main hormone regulating calcium phosphate homeostasis and mineral bone metabolism. Loss of muscle mass and fraity are prevalent in many chronic disease. The study group consisted of 31 samples from Institut Kesehatan Medistra (n=12) and Fakultas Kedokteran Gigi USU (n=19). Vitamin D levels were measure with ELISA (Diagnostic Biochem Canada). Most age for student Institut Kesehatan Medistra is 21 years old and student Fakultas Kedokteran Gigi USU is 22 years old. Frequency of Vitamin D level student Institut Kesehatan Medistra is 91,7% (deficiency) and student Fakultas Kedokteran Gigi USU 84,2%(deficiency). There was significant difference in vitamin D levels between two group with significant (p=0,018).
PENGARUH PEMBERIAN D-GALAKTOSA TERHADAP PERUBAHAN BERAT BADAN MENCIT JANTAN Indah Permata Sari; Muhammad Ichwan; Yahwardiah Siregar
JURNAL KEPERAWATAN DAN FISIOTERAPI (JKF) Vol 2 No 2 (2020): Jurnal Keperawatan dan Fisioterapi (JKF)
Publisher : Fakultas Keperawatan dan Fisioterapi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.105 KB) | DOI: 10.35451/jkf.v2i2.336

Abstract

D-galactose is aldohexose which occurs naturally in the body, including in the brain which is found in lactose disaccharides in honey, beets and milk products. Needed in the body for lactose formation during the process of lactogenesis. Provision of large amounts of D-galactose can cause oxidative damage to various tissues and organs. The purpose was to see changes in body weight in male rats after being given a D-galactose injection. Samples using male mice aged 8 weeks with a weight of ± 30 grams, the number of samples was 6 mice swiss webster strain. intra-peritoneal injection of D-galactose is given for 6 weeks every day (150 mg / kg / bb). The results of this study indicate that the statistical analysis is significant p <0.05 (p = 0.016). The conclusion after being given injection of D-galactose for 6 weeks every day intra peritoneally was found D-galactose had an effect on the weight gain of male mice.
PERAN POLIMORFISME GEN MATRIKS METALLOPROTEINASE-9 DENGAN KEJADIAN PENYAKIT PARU OBSTRUKTIF KRONIK Wilson Arthur Zein; Yahwardiah Siregar; Amira P Tarigan
JURNAL KEPERAWATAN DAN FISIOTERAPI (JKF) Vol 3 No 1 (2020): Jurnal Keperawatan dan Fisioterapi (JKF)
Publisher : Fakultas Keperawatan dan Fisioterapi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jkf.v3i1.427

Abstract

Excessive secretion of MMP-9 is one of the cause of COPD is which can cause undesirable lung tissue degradation. Increased expression of MMP-9 is associated with increased gene promoter activity. The purpose of this study was to determine the role of mettaloproteinase-9 matrix gene polymorphisms with COPD events. This was a case-control study. With a total sample of 60 patients consisting of 30 COPD patients and 30 non-COPD patients. Samples were amplified by PCR (Polymerase Chain Reaction) The results of DNA amplification were digested with SphI restriction enzyme to see the allele variant of MMP-9 C-1562T. Visualization of restriction results with 2% agarose gel electrophoresis will show 2 bands on the T allele (188bp, 247bp), 1 band on the C allele (435bp). The results obtained were homozygous CC variants in COPD 20 (66.67%) and non-COPD 21 (70%). Heterozygous variant of CT 10 (33.33%) in COPD and 9 (30%) in non-COPD. TT variant is not found at all. Based on the results obtained by the most CC homozygous variants in COPD (66.67%) and non-COPD (70%) and no association was found between MMP-9 polymorphism and COPD events (p> 0.05). Based on the results of this study, there is no significant relationship that we can conclude.
STUDI PRELIMINARI TENTANG PENGARUH D-GALAKTOSA DALAM MENGINDUKSI STRES OKSIDATIF PADA MENCIT JANTAN GALUR OUTBRED FK USU Siti Sarah Bintang Sarah Bintang; Yahwardiah Siregar; Muhammad Ichwan
Jurnal FARMASIMED (JFM) Vol 2 No 1 (2019): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.922 KB) | DOI: 10.35451/jfm.v2i1.191

Abstract

Abstract Oxidative stress occurs due to imbalance of free radicals over antioxidant level in the body. This condition causes lipid peroxidation and DNA damage. D-Galaktosa is The mechanism of oxidative stress induced by d-galactose occurs in the subcellular, especially in the brain's mitochondria. Increasing the concentration of d-galaktosa is oxidized by galaktosa oxidase to form hydrogen peroxide (H202) which causes a decrease in superoxide dismutase (SOD). H202 reacts with reduced iron and forms hydroxide ions (OH-). Objective: The aim of this study was to determine the effect of d-galaktosa induction on oxidative stress levels (MDA) in male mice. Methods: Methods of samples of mice given d-galaktosa and blood collection from the heart were carried out at the Pharmacology Laboratory of the Faculty of Medicine, University of North Sumatra. Results: The results showed that administration of d-galaktosa, through intraperitonial injection every day for 6 weeks, had an effect on the levels of oxidative stress in male mice. Conclusion: The results of this study indicate that administration of d-galaktosa, through intraperitonial injection every day for 6 weeks, has an effect on levels of oxidative stress in male mice.
EFEK LATIHAN FISIK TERHADAP KADAR MALONDIALDEHID PADA HEWAN MODEL PENUAAN Indah Permata Sari1; Muhammad Ichwan; Yahwardiah Siregar
Jurnal Endurance Vol. 6 No. 1 (2021): Jurnal Endurance : Kajian Ilmiah Problema Kesehatan
Publisher : LLDIKTI Wilayah X

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.772 KB) | DOI: 10.22216/jen.v6i1.134

Abstract

Aging is a natural process that is characterized by decreased function. In the aging process a decline in the ability of reactive oxygen species. This causes damage to the membrane lipids that cause a decrease in function of the body's organs. Lipid oxidation process in the membrane because free radicals produce lipid peroxidation such as malondialdehyde which is used as a marker of oxidative stress. Regular physical exercise can overcome the negative effects of oxidative stress. To conduct studies on aging, animal models are used. One of the animal models used is the aging induction model by giving D-galactose. Metabolites from d-galactose can induce aging by causing oxidative stress. The purpose of this study was to determine the effect of physical exercise on reducing malondialdehyde levels in an aging animal model induced by D-galactose. This research was experimental in-vivo with a post test only research design. The research sample of 8 weeks old swiss webster mice, consisted of 10 treatment groups. The results of studies on animal models of aging induced D-galactose on malondialdehyde levels after being treated for 6 weeks from the statistical results obtained p value ≥ 0.05 was not significant. However, there was a significant decrease in MDA levels in the D-galactose + physical exercise group
EFEK LATIHAN FISIK TERHADAP KADAR MALONDIALDEHID PADA HEWAN MODEL PENUAAN Indah Permata Sari1; Muhammad Ichwan; Yahwardiah Siregar
Jurnal Endurance Vol. 6 No. 1 (2021): Jurnal Endurance : Kajian Ilmiah Problema Kesehatan
Publisher : LLDIKTI Wilayah X

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.772 KB) | DOI: 10.22216/jen.v6i1.134

Abstract

Aging is a natural process that is characterized by decreased function. In the aging process a decline in the ability of reactive oxygen species. This causes damage to the membrane lipids that cause a decrease in function of the body's organs. Lipid oxidation process in the membrane because free radicals produce lipid peroxidation such as malondialdehyde which is used as a marker of oxidative stress. Regular physical exercise can overcome the negative effects of oxidative stress. To conduct studies on aging, animal models are used. One of the animal models used is the aging induction model by giving D-galactose. Metabolites from d-galactose can induce aging by causing oxidative stress. The purpose of this study was to determine the effect of physical exercise on reducing malondialdehyde levels in an aging animal model induced by D-galactose. This research was experimental in-vivo with a post test only research design. The research sample of 8 weeks old swiss webster mice, consisted of 10 treatment groups. The results of studies on animal models of aging induced D-galactose on malondialdehyde levels after being treated for 6 weeks from the statistical results obtained p value ≥ 0.05 was not significant. However, there was a significant decrease in MDA levels in the D-galactose + physical exercise group