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2. Characterization Of Lactic Acid Bacteria (LAB) Origin Sumatran Orangutan (Pongo abelii) In Zoo Bukittinggi Based On Analysis 16 S rRNA Siti Hajar; Safika Safika; Darmawi Darmawi; Wenny Novita Sari; Erdiansyah Rahmi; Yandi Syahputra; Nurliana Nurliana; Rinidar Rinidar
The International Journal of Tropical Veterinary and Biomedical Research Vol 1, No 2 (2016): Vol. 1 (2) November 2016
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.484 KB) | DOI: 10.21157/ijtvbr.v1i2.5944

Abstract

The purpose of this study was to characterize the bacterial 16S rRNA gene of Sumatran orangutan (Pongo abelii) Bukittinggi West Sumatera zoo. The sample used in this study are lactic acid bacteria of the Sumatran orangutan (Pongo abelii) derived from zoo Bukittinggi West Sumatra Indonesia. This study was an exploratory study that conducted at the Laboratory through several stages. The first stage was the isolation of LAB from faeces of Sumatran orangutans using MRS agar medium and then cultured in a liquid medium NB. The next stage was the isolation of total DNA, and then, the third stage was the amplification of the 16S rRNA gene and agarose gel electrophoresis. Then,in the fourth stage of determining the DNA sequence and analysis of DNA sequence homology. The final stage was the computational analysis of 16S rRNA gene Sumatran orangutan (Pongo abelii). The results showed that lactic acid bacteria (LAB) from the Sumatran orangutan (Pongo abelii) is close to lactic acid bacteria Lactobacillus helveticus strain IMAU50151 with the levelof similarity of 89%. It is possible that these bacteria is a new species or the species that LAB has not been reported in Genbank.
SURVEI PENGETAHUAN DAN SIKAP PEMILIK RUMAH MAKAN TERHADAP RANTAI PENGOLAHAN DAGING AYAM DI BANDA ACEH (Survey of Knowledge and Attitude of Restaurant Owners on Chicken Processing Chain in Banda Aceh) laras berlian xarolin; razali razali; ismail ismail; rastina rastina; Muhammad jalaluddin; darmawi darmawi
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 2 (2017): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.767 KB) | DOI: 10.21157/jim vet..v1i2.2893

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ABSTRAKPenelitian ini bertujuan mengetahui pengetahuan dan sikap pemilik rumah makan terhadap rantai pengolahan daging ayam di Banda Aceh. Penelitian ini dilaksanakan pada bulan Januari 2017 di beberapa rumah makan di Banda Aceh. Penelitian dilakukan dalam bentuk survei lapangan dengan mewawancarai responden menggunakan kuesioner terstruktur. Responden dipilih secara proporsional terhadap pemilik rumah makan di Banda Aceh. Kriteria responden meliputi berumur ≥ 20 tahun, pemilik rumah makan yang menjual olahan daging ayam, rumah makan dengan kebutuhan daging ayamnya ≥ 20 ekor/hari dan pemilik rumah makan di lokasi penelitian. Data hasil penelitian dianalisis secara deskriptif. Hasil penelitian terhadap 45 responden menunjukkan bahwa pengetahuan pemilik rumah makan terhadap rantai pengolahan daging ayam tergolong baik, namun sikap pemilik rumah makan terhadap rantai pengolahan daging ayam tergolong kurang baik, karena 22,22% responden menggunakan alat potong (pisau) yang sama dengan sayuran, serta 48,89% responden tidak mencuci tangan sebelum mengolah daging ayam.Kata kunci : pengetahuan, sikap, pemilik rumah makan, daging ayam, rantai pengolahan.ABSTRACT This study was aims to determine the knowledge and attitude of restaurant owners on chicken meat processing chain in Banda Aceh. This study was conducted in January 2017 in several restaurants in Banda Aceh. This research was conducted in the form of a field survey by interviewing respondents using a structured questionnaire. Respondents were selected proportionally which is restaurant owners in Banda Aceh. The criteria of respondents were aged ≥ 20 years, restaurant owners who sell chicken, restaurants that needs ≥ 20 chicken / day and restaurant owners in the study site. Data were analyzed descriptively. The results of 45 respondent shows that the knowledge of restaurant owners on chicken meat processing chain is quite good, but the majority of owners attitude towards chicken meat processing chain relatively poor because 22,22% of respondents using the same knife with vegetables and 48,89% of respondents do not wash their hands before treating chicken meat.Keyword : knowledge, attitude, restaurant owners, chicken meat, processing chain.
10. Isolation and identification of Aeromonas sp in goldfish (Carrasius auratus) from several pet shops in Banda Aceh Hamny Hamny; Muhammad Zulfan; Mahdi Abrar; Maryulia Dewi; Darmawi Darmawi; Razali Daud; Budianto Panjaitan
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (635.519 KB) | DOI: 10.21157/j.med.vet..v13i1.4225

Abstract

The aim of this study was to isolate and identify Aeromonas sp from pet shops in Banda Aceh. A total of 15 goldfish were obtained from 3 pet shop in Banda Aceh and examined at the Laboratory of Microbiology, Faculty of Veterinary Medicine, University of Syiah Kuala in Banda Aceh. Isolation of bacteria obtained from liver, kidneys and spleen were cultured in the media Rimler-Shotts (RS) Medium Base. The bacteria were identified based on morphology of the growing bacteria, Gram stain, IMViC test, and biochemical tests. The results showed that there were no Aeromonas sp in goldfish (Carrasius auratus) from three pet shops in Banda Aceh.
TOTAL BAKTERI ASAM LAKTAT (BAL) PADA CAECUM PUYUH (Coturnix japonica) Annisah putri Ananda Nasution; Erina Erina; Darmawi Darmawi
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.308 KB) | DOI: 10.21157/jim vet..v1i4.5470

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TOTAL BAKTERI ASAM LAKTAT (BAL) PADA CAECUM BURUNG PUYUH (Coturnix japonica )  ABSTRAKPenelitian ini bertujuan untuk mengetahui total bakteri asam laktat (BAL) pada caecum puyuh (Coturnix japonica). Sampel yang digunakan adalah ± 1 gram isi caecum dari 6 ekor puyuh yang terdiri dari tiga ekor puyuh jantan dan tiga ekor puyuh betina. Penghitungan total koloni bakteri dilakukan dengan metode Total Plate Count (TPC). Bakteri di tanam pada media MRSA (De Man Rogosa Sharpe Agar) dengan metode spread plate (cawan sebar) setelah dilakukan pengenceran desimal dengan pengenceran 10-1-10-7. Koloni yang tumbuh diamati morfologi koloninya (warna, bentuk, pinggiran, diameter koloni dan elavasi) selanjutnya dilakukan pewarnaan Gram. Hasil penelitian menunjukkan bahwa jumlah BAL, pada caecum puyuh jantan adalah 3,06x107 CFU/ml sedangkan pada puyuh betina adalah 21,4 x106 CFU/ml. Dari hasil penelitian dapat diketahui bahwa jumlah bakteri asam laktat (BAL) pada puyuh jantan lebih tinggi dibandingkan pada puyu  h betina. Oleh karena itu dapat disimpulkan bahwa jumlah BAL pada puyuh jantan lebih banyak dari pada puyuh betina.The Amount Of Lactic  Acid Bacteria (LAB) In Caecum Of The Quail’s (Coturnix japonica)                                             ABSTRACTThis research was concluted to count the total of lactic acid bacteria in the caecum of quail’s. About 1 gram of fluid from the caecum of three male and three female quail’s were used for this study. The total of lactic acid bacteria in caecum count based on total plate count (TPC) method. The bacteria are inconculated in MRSA(De Man Rogosa Sharpe Agar) with spread plate method after serial dilution dilution (10-1-10-7) were prepared. Colony that grow in MRSA were examined for morfology colony and Gram’s stain. The result show the total of lactic acid bacteria in male quail’s is 3,06 x107CFU/ml and in female quail’s is 21,4 x106 CFU/ml. In conclution, the total colony of lactic acid bacteria of male quail’s is higher than in female quail’s.  
The Presence of Salmonella in Sie Balu, Acehnese Dried Meat After Gamma Irradiation Husna Husna; Nurliana Nurliana; Darmawi Darmawi
The International Journal of Tropical Veterinary and Biomedical Research Vol 2, No 1 (2017): Vol. 2 (1) May 2017
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.097 KB) | DOI: 10.21157/ijtvbr.v2i1.8632

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Sie balu is an Acehnese dried meat preserved by the addition of salt, acid and dried. However, long processing and drying it under the sun can cause meat products contaminated by Salmonella. Irradiation can eliminate  Salmonella  and  other  pathogenic  bacteria  in  food.  This  study  aims  to  determine  the  presence  of Salmonella in sie balu after irradiated with increasing doses and 2 to 4 months shelf life. Sie balu was made of 5 kg fresh beef, dried under the sun to dry, vacuum and irradiated at doses of 5, 7 and 9 kGy. Salmonella was detected using bacteriological and biochemical tests. Results showed sie balu contaminated by Salmonella paratyphi B and Salmonella cholerasuis. The irradiation and shelf life significantly affect (P0.05) the count of Salmonella in sie balu compared with unirradiated one. Irradiation doses of 5, 7 and 9 kGy do not affect the count of Salmonella in sie balu. Extending the shelf life up to 4 months can increas the count of Salmonella. This study concluded that irradiated sie balu at doses of 5, 7 and 9 kGy can be stored for up to two months
The Effect Of Cellulase Content On Phytoestrogens Formation (Genistein) Materials On Fermented Soybean Waste Aspergillus niger Vara Tassa Sutari; Nurliana Nurliana; Sugito Sugito; Darmawi Darmawi; Rinidar Rinidar
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 1 (2018): Vol. 3 (1) May 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.805 KB) | DOI: 10.21157/ijtvbr.v3i1.11362

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The activity of the cellulase enzyme and the content of phytoestrogen (genistein) forming is determined during the fermentation of soybean waste with Aspergullus niger. The analysis is performed at fermentation incubation time of 0, 48, 96, 144 and 192 hours and treatment with addition of micro nutrient (V2) and without micro nutrient (V1). Data analysis on cellulase enzyme activity on phytoestrogen forming material is performed using T test. The average of cellulase content of fermented soybean waste increases from 0.032-0.139 IU / ml (V1) and 0.061-0.158 IU / ml (V2) after 48 hours into 0.238 IU / ml (V1) and 0.245 IU / ml (V2) after 192 hours The average of genestein levels in the fermented soybean waste increases as well from 0.101 - 0.573 mg / g (V1) and 0.114 - 0.587 mg / g (V2) after 48 hours and increase into 0.722 mg / g (V1) and 0.699 mg / g (V2) after 192 hours. Statistical analysis indicates that the treatment of V1 and V2 has an effect on the increase of cellulase enzyme content and genistein level (P 0.05). The correlation between enzyme activity of cellulase and genistein on soybean waste fermented with Aspergillus niger is significant (R2 = 0,949). The relationship between the enzyme performance and its formation material is running synergistic, cellulase enzyme activity and genistein level experience significant increase during the fermentation process.
Mucosal Mast Cells Contribution in Intestinal Defense of Chickens (Gallus domesticus) Infected Naturally by Ascaridia galli Ummu Balqis; Masda Admi; Darmawi Darmawi; Muhammad Hambal
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.91 KB)

Abstract

This study was aimed at finding out the investigation of mucosal mast cells   in intestines of chicken  that  were  naturally  infected  by  Ascaridia galli.  Amount  of  ten  intestine  of  freshly slaughtered chickens (Gallus domesticus) found from local abatoir in Banda Aceh were divided into two groups containing five intestines of each. Mucosal mast cells count were done of which histologic slides were made in stained serial histological sections with Alcian blue (pH 0,3) and Safranin-O (pH 0,1) of the intestines. The result showed that the mucosal mast cells increased significantly (P 0.05) in intestines of chickens infected naturally by survival A. galli adult worms. It was concluded that the intestinal defense of chickens against parasite infection is associated with the mucosal mast cells contribution by creating an environment hostile to the establishment and survival of intestinal nematodes, A. galli.
isolasi dan identifikasi bakteri gram negatif pada ambing sapi aceh rina dwita; Teuku Zahrial Helmi; Darmawi Darmawi; Abdullah Hamzah
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 4 (2018): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.554 KB) | DOI: 10.21157/jim vet..v2i4.9015

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Penelitian ini bertujuan untuk mengidentifikasi bakteri Gram negatif pada ambing sapi aceh, dengan mengisolasi bakteri yang berasal dari 10 swab ambing sapi aceh yang tumbuh pada media NB, media Mac Conkey, SSA dan PAB. Selanjutnya dilakukan pewarnaan Gram, pengamatan morfologi koloni dan uji biokimia. Hasil yang diperoleh yaitu didapatkan 3 bakteri. Bakteri A merupakan  Shigella boydii dengan ciri berbentuk batang, koloni berwarna kuning pada media SSA, tembus cahaya, mampu memecahkan asam amino triptofan, hanya mampu menurunkan pH menjadi asam, memfermentasikan manitol dan dubius pada fermentasi sukrosa. Bakteri B merupakan Enterobacter cloacae dengan ciri berbentuk batang, koloni berwarna merah muda pada media SSA, berflagel, hanya mampu meningkatkan pH menjadi basa, memfermentasikan gula (manitol, laktosa dan sukrosa). Bakteri C merupakan Enterobacter aerogenes dengan ciri berbentuk batang, koloni berwarna putih pada media PAB, berflagel, hanya mampu meningkatkan pH menjadi basa dan memfermentasikan gula (manitol dan sukrosa). Kesimpulan dari penelitian ini berdasarkan Tabel Cowan and Steel’s (1993) didapatkan bakteri dengan spesies : Shigella boydii, Enterobacter cloacae dan Enterobacter aerogenes.This study aims to identify Gram negative bacteria on aceh cow udders, by isolating bacteria from 10 growing aceh cow udder swabs on Nutrient Broth media, Mac Conkey, SSA and PAB media. Then Gram staining is done, morphological observations of colonies and biochemical tests. The results were obtained 3 bacteria. Bacteria A is Shigella boydii with trunk shaped, yellow colony on SSA media, translucent, able to break tryptophan amino acids, only capable decreases pH to acid, ferments mannitol and dubius in sucrose fermentation. Bacteria B is Enterobacter cloacae with trunk shaped, colored colonies pink on SSA media, flaked, only able to increase pH to alkaline, ferment sugar (mannitol, lactose and sucrose). Bacteria C is Enterobacter aerogenes with a rod-shaped characteristic, white colony on PAB media, flaked, only able to increase pH to become alkaline and ferment sugar (mannitol and sucrose). The conclusion of this study is based on Cowan and Steel’s Table (1993) bacteria found with species: Shigella boydii, Enterobacter cloacae and Enterobacter aerogenes.
ISOLASI DAN IDENTIFIKASI BAKTERI Staphylococcus aureus PADA VAGINA SAPI ACEH (Isolation and Identification of Staphylococcus aureus Bacteria in Vagina of Aceh Cattle) siti hajar; Teuku Zahrial Helmi; Darmawi Darmawi; Al Azhar; Fakhrurrazi Fakhrurrarzi; Azhar Azhar
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.04 KB) | DOI: 10.21157/jim vet..v2i3.8197

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Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Staphylococcus aureus pada vagina sapi aceh. Penelitian menggunakan sampel berupa 10 swab vagina sapi aceh. Penelitian ini adalah penelitian deskriptif dengan menggunakan metode Carter, spesimen untuk pemeriksaan bakteri berupa hasil swab vagina sapi aceh yang di ambil dengan menggunakan swab steril. Spesimen yang diperoleh dimasukkan ke dalam tabung reaksi yang berisi nutrient broth (NB) sebagai media perkembangbiakan bakteri. Selanjutnya dilakukan isolasi pada media manitol salt agar (MSA) sebagai media selektif, identifikasi bakteri menggunakan pewarnaan Gram, uji katalase, uji hemolisa dan uji biokimia (manitol dan glukosa). Hasil penelitian melalui isolasi pada media manitol salt agar (MSA) adalah pertumbuhan koloni bakteri berwarna kuning keemasan, berwarna ungu pada pewarnaan Gram yang menandakan bakteri Gram positif, berbentuk kokus, uji katalase positif, uji hemolisa menghasilkan beta (β) hemolisis, dan mampu memfermentasi pada uji glukosa dan manitol. Kesimpulan dari penelitian ini bahwa dari 10 sampel swab vagina sapi aceh positif diisolasi dan diidentifikasi bakteri Staphylococcus aureus.This study was conducted to isolate and identify the Staphylococcus aureus bacteria in vagina of Aceh Cattle. This study used 10 swab vaginal samples from 10 female Aceh Cattle. Data obtained were analyzed descriptively using Carter method, the specimen of bacteria had taken by using sterile swab. The result of specimens were fed into a test tube containing nutrient broth (NB) as a bacterial growth media. Furthermore, the isolation was done on manitol salt agar (MSA) media as selective media, bacterial identification were used Gram staining, catalase test, and biokimia test (mannitol and glucose). The results of research through isolation on manitol salt agar (MSA) media was a golden yellow colony, had purple color in Gram stain that signified Gram positive bacteria, coccus-shaped, catalase test positive, produced beta (β) hemolysis in hemolysis test and able to ferment on glucose and mannitol tests. This research conclude that Staphylococcus aureus bacteria were positively identified from vaginal swabs of aceh cattle.
5. Isolation and Identification of Salmonella sp in spleen of male layer chicken in Sibreh farms, Aceh Besar Erina Erina; Mahdi Abrar; Balqis Alrasyid Suyoto; Maryulia Dewi; Darmawi Darmawi; Darniati Darniati; Muttaqien Bakri
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.108 KB) | DOI: 10.21157/j.med.vet..v11i1.3986

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This study aimed to isolate and identify the bacteria Salmonella sp in spleen of male layer chicken that died in farms Sibreh, Aceh Besar. A sample of the research is the spleen of male layer chickens. Isolation of Salmonella sp performed by inoculating spleen that were pierced using a swab on the media Selenite Cystine Broth (SCB) then planted on selective media Salmonella Shigella Agar (SSA) and colony morphology was observed, continued with observation of Gram stain under microscopic. Furthermore, colonies of suspected Salmonella sp planted in the media MR-VP (Oxoid), Indol (Difco), Simmons's Citrate Agar (Oxoid), Triple Sugar Iron Agar (TSIA), Sulfite Indol Motiliti (SIM), and media sugars (sucrose, manitol, glucose, lactose , maltose). The results of this study were analyzed descriptively. The research shows a results from a total of 33 samples, we found that 15 samples are positive that can be isolated and identified as Salmonella sp. Therefore it can be concluded that Salmonella sp is possible the death cause of male layer chicken in farms Sibreh, Aceh Besar