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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal Medika Veterinaria Jurnal Ilmiah Mahasiswa Veteriner INVOTEK: Jurnal Inovasi Vokasional dan Teknologi The International Journal of Tropical Veterinary and Biomedical Research

Darmawi Darmawi

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Author-ID : 2567720

Automotive Engineering Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Energy Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Transportation Veterinary Other

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Karakteristik Nilai Kalor Briket Campuran Tebu Tibarau dan Kulit Durian Hendri Nurdin; Waskito Waskito; Darmawi Darmawi
invotek Vol 19 No 1 (2019): INVOTEK: Jurnal Inovasi, Vokasional, dan Teknologi
Publisher : Universitas Negeri Padang

Increased energy needs in the community can have an impact on the scarcity of fuel oil so that it becomes a dependence on energy as a main requirement. The development of product diversification in the form of mixed sugar cane and durian skin briquettes in an effort to obtain alternative fuels. Renewable energy sources that have the potential to be used as fuel for briquettes made from raw sugar cane and durian skin. Efforts and innovations of mixed sugar cane and durian skin briquettes are produced as alternative fuels so that they have characteristics of quality and performance, namely the calorific value. The stages of testing and evaluation of mix sugar cane and durian skin briquettes are needed before being developed and recommended as alternative fuels. Through the technology of the process of making briquette products with compacting and optimizing the content composition and use of adhesive types to obtain the heating value. From this study can be produced mixed sugar cane and durian skin briquettes which are recommended as alternative fuels. The highest characteristics of the heating value of mixed of sugar cane and durian skin briquettes with gambir adhesive were obtained at 16967.91 kJ/Kg and density of 682.23 Kg/m3. Briquettes (biomass) that have a high density have a high calorific value. Comparison of the composition of the mixture in making briquettes is 40%: 40%: 20%. The composition of the mixture between the raw material and the adhesive, the amount of grain, the force of compacting greatly affects the characteristics of the calorific value and density of the briquettes.

Histamine Levels in Repeated Thawing Beef Nurliana Nurliana; Borgo Mauly Nasution; Darmawi Darmawi; Sugito Sugito
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.256 KB)

The aim of this research was to determine the histamine levels in repeated thawing beef. This research were using longisimus dorsi part (sirloin) of beef. Repeated thawing treatments of beef consisted of P1 as control without freezing, P2 as first thawing after one week freezing, P3 as second thawing without freezing two weeks and P4 with freezing three weeks. The slices of beef was storage in freezer at temperature of -20ºC every weeks for three weeks. The thawing process was done by storage in refrigerator at temperature of 6ºC for 270 minutes after being removed from the storage in freezer every weeks for three weeks. PIC (Protease Inhibiting Cocktail) methode were used to extraction of enzyme in beef. Enzyme Link Imunosorbent Assay (ELISA) were used to detected the histamine levels with 450 nm wave length. The quantitative data from parameters were analyzed by descriptive. The results of the study concluded that repeated thawing beef in refrigerator at temperature of 6ºC could decrease the histamine levels.

Mucosal Mast Cells Contribution in Intestinal Defense of Chickens (Gallus domesticus) Infected Naturally by Ascaridia galli Ummu Balqis; Masda Admi; Darmawi Darmawi; Muhammad Hambal
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.91 KB)

This study was aimed at finding out the investigation of mucosal mast cells in intestines of chicken that were naturally infected by Ascaridia galli. Amount of ten intestine of freshly slaughtered chickens (Gallus domesticus) found from local abatoir in Banda Aceh were divided into two groups containing five intestines of each. Mucosal mast cells count were done of which histologic slides were made in stained serial histological sections with Alcian blue (pH 0,3) and Safranin-O (pH 0,1) of the intestines. The result showed that the mucosal mast cells increased significantly (P 0.05) in intestines of chickens infected naturally by survival A. galli adult worms. It was concluded that the intestinal defense of chickens against parasite infection is associated with the mucosal mast cells contribution by creating an environment hostile to the establishment and survival of intestinal nematodes, A. galli.

Inhibition of Salmonella sp. Growth in Comparison with Antibiotics and Pineapple (Ananas comosus) Squeeze Masda Admi; Erina Erina; Azhari Azhari; Ummu Balqis; Muhammad Hambal; Darmawi Darmawi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1063.056 KB)

The purpouse of this study to examine antibiotics inhibition growth of Salmonella sp. isolated from feces of broiler chickens to chloramphenicol, gentamicin, and tetracycline in coparison with pineapple squeeze. Swab sample from broiler chicken cloaca was cultured to nutrient broth media and incubated at 37°C temperature for 24 hours. Culture was compared with Mc Farland 3 standardization, and separated on surface of Muller Hinton Agar (MHA). Antibiotic sensitivity was tested by mean of chloramphenicol, gentamisin, and tetrasiklin in coparison with pineapple squeeze. The antibiotic disks (Oxoid) were put on the surface of MHA media which had inoculated with Salmonella sp. bacteria. Antibiotic sensitivity was determined based on formation of inhibition zone in surrounding of antibiotic disc. The result showed that Salmonella sp. bacteria growth was inhibited in ≥ 21 mm and ≥ 18 mm, ≥ 7 mm and ≥ 9 mm against chloramphenicol, gentamisin, tetrasiklin, and pineapple squeeze respectively. This research concluded that the Salmonella sp. cultured in this manner were sensitive to chloramphenicol and gentamisin. The inhibition growth of Salmonella sp. caused by chloramphenicol and gentamicin was significantly higher in comparison with pineapple (Ananas comosus) squeeze, meanwhile the Salmonella sp. bacteria was resistance against tetracycline. Our result suggest that both antibiotics chloramphenicol and gentamicin were recommended for antibiotic therapy in Salmonella sp. infection.

9. The Isolation of Steptococcus sp on horse’s dental caries in Aceh Tengah Regency Sagasias Ahmad; Erina Erina; M Daud AK; Darmawi Darmawi; Fakhrurrazi Fakhrurrazi; M. Isa
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

The purpose of this study was to detect the existence of the Streptococcus sp bacteria in the horses’ dental caries in Aceh Tengah Regency. The caries samples used in this study were taken from 8 horses in Aceh Tengah Regency. The samples were collected using swab on the teeth and then put into Nutrient Broth (NB) media and further were cultured on selective media TSY20B. The bacteria identification was conducted by observing the bacteria’s morphology, Gram staining and biochemical test. The eight samples of the horses’ dental caries showed that Streptococcus sp bacteria could be isolated from all of the collected samples. From this research it could be concluded that Streptococcus sp bacteria was found in the horses’ dental caries and it was one of the causes of the dental caries.

9. Total colony of cellulolitic bacteria in the rumen of aceh cattle Safika Safika; Siti Wardianah Matondang; Darmawi Darmawi; Mahdi Abral; Erina Erina; M Jalaluddin
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

The aim of this study was to count the total sum of cellulolitic bacteria colony in aceh cattle rumen. The sample used was aceh cattle rumen liquid which taken from salughtered house in Banda Aceh. Aceh cattle rumen liquid were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Petri dish was entered to candle jar to be incubated in an incubator with temperature 37oC about 96 hours. Colony of cellulolitic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of colony), counting total of bacteria which was grown, and Gram staining. The results showed that there is a white translucent colony morphology, growth inside agar, smooth periphery, Gram-positive bacil. The average number bacteria of total bacteria in the cattle rumen liquid was 4,81 x 105 cfu/ml

5. Isolation and Identification of Salmonella sp in spleen of male layer chicken in Sibreh farms, Aceh Besar Erina Erina; Mahdi Abrar; Balqis Alrasyid Suyoto; Maryulia Dewi; Darmawi Darmawi; Darniati Darniati; Muttaqien Bakri
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

This study aimed to isolate and identify the bacteria Salmonella sp in spleen of male layer chicken that died in farms Sibreh, Aceh Besar. A sample of the research is the spleen of male layer chickens. Isolation of Salmonella sp performed by inoculating spleen that were pierced using a swab on the media Selenite Cystine Broth (SCB) then planted on selective media Salmonella Shigella Agar (SSA) and colony morphology was observed, continued with observation of Gram stain under microscopic. Furthermore, colonies of suspected Salmonella sp planted in the media MR-VP (Oxoid), Indol (Difco), Simmons's Citrate Agar (Oxoid), Triple Sugar Iron Agar (TSIA), Sulfite Indol Motiliti (SIM), and media sugars (sucrose, manitol, glucose, lactose , maltose). The results of this study were analyzed descriptively. The research shows a results from a total of 33 samples, we found that 15 samples are positive that can be isolated and identified as Salmonella sp. Therefore it can be concluded that Salmonella sp is possible the death cause of male layer chicken in farms Sibreh, Aceh Besar

10. Isolation and identification of Aeromonas sp in goldfish (Carrasius auratus) from several pet shops in Banda Aceh Hamny Hamny; Muhammad Zulfan; Mahdi Abrar; Maryulia Dewi; Darmawi Darmawi; Razali Daud; Budianto Panjaitan
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

The aim of this study was to isolate and identify Aeromonas sp from pet shops in Banda Aceh. A total of 15 goldfish were obtained from 3 pet shop in Banda Aceh and examined at the Laboratory of Microbiology, Faculty of Veterinary Medicine, University of Syiah Kuala in Banda Aceh. Isolation of bacteria obtained from liver, kidneys and spleen were cultured in the media Rimler-Shotts (RS) Medium Base. The bacteria were identified based on morphology of the growing bacteria, Gram stain, IMViC test, and biochemical tests. The results showed that there were no Aeromonas sp in goldfish (Carrasius auratus) from three pet shops in Banda Aceh.

10. Isolation and Identification of Lactic Acid Bacteria Genus Pediococcus from Sumatran Orangutan (Pongo abelii) faeces at Kandi Zoo and Kinantan Zoo West Sumatera Resti Reimena; Erina Erina; Darniati Darniati; Fakhrurrazi Fakhrurrazi; Darmawi Darmawi; Hamdani Budiman
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

This research has purposes to isolating and identifying the lactic acid bacteria genus Pediococcus in sumatran orangutan (Pongo abelii) feaces. Feaces are isolated from one orangutan at Kandi Zoo and two orangutans at Kinantan Zoo. The isolating of the lactic acid bacteria was done by using streak plate method with the MRSA (de Man Rogosa Sharpe Agar) specific media. Identification was done by the morphological observing, Gram stain, catalase test, oxidase test and biochemical test. The result showed that one isolate feaces from Kandi Zoo has cocci shaped with the isolate prediction approaches genus Pediococcus sp. The characteristic features of isolate are cocci shaped, Gram positive, tetrad cell structure, nonmotile, catalase negative, and oxidase negative. Based on these characteristics, it can be concluded that there is a lactic acid bacteria coloni which grouped into genus Pediococcus in male orangutan feaces from Kandi Zoo

PENGARUH PENCELUPAN KARKAS AYAM PEDAGING DALAM LARUTAN ASAM SITRAT DAN ASAM ASETAT TERHADAP ANGKA LEMPENG TOTAL Escherichia coli (The Immersion Effect of Broiler Carcass in Citric Acid and Acetic Acid on the Total Plates Count of Escherichia Coli) Nurliana Nurliana; Sandy Cakra Yuda; Faisal Jamin; Teuku Reza Ferasyi; M. Isa; Darmawi Darmawi
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

This study aimed to determine the immersion effect of broiler carcasses in 3% citric acid, 3% acetic acid, and combination of citric acid and acetic acid with concentration of 1.5% each on the total plate count of bacteria E. coli. Eight broilers carcasses (dissected into 2 parts) were divided into 4 treatment groups with 2 times repetition and 3 times observation at 0, 4, and 8 hours after dipped. The control treatment (K0) without dipped into acid solution. Broiler carcasses in the first (K1), second (K2), and the third (K3) treatments were dipped into 3% acetic acid, 3% citric acid, and combination of 1.5% acetic acid and 1.5% citric acid respectively. Data were analyzed using analysis of variance. The average of E. coli count in K0 at 0, 4, and 8 hours post dipping were 5.2±0.061, 6.8±0.99, and 7.0±0.107 log 10 cfu/g of chicken meat respectively. In similar observation time with K0, the average of total E. coli count were 5.5±0.373, 6.0±0.023, and 6.0±0.242 log 10 cfu/g of chicken meat in K1, 5.3±0.166, 6.0±0.143, and 6.0±0.084 log 10 cfu/g of chicken meat in K2, 5.7±0.041, 5.9±0.92, and 5.8±0.058 log 10 cfu/g of chicken meat in K3 at 0,4, and 8 hours post dipping respectively. Statistical analysis showed that the immersion of broiler carcass in acetic acid and citric acid solution were significantly (P 0.05) decrease the number of E. coli. There were interaction between immersion of broiler carcass in organic acids and observation time which significantly decrease the number of E. coli (p 0.05). In conclusion, the immersion of carcass chicken in 3 % acetic acid, 3% citric acid, and their combination reduce the amount of E. coli. Key words: chicken carcass , citric acid 3%, acetic acid 3% , Escherichia coli

Co-Authors . Darniati . Nurliana Abdullah Hamzah Admi, Masda Al Azhar Amalia Sutriana Annisah putri Ananda Nasution Azhar Azhar Azhari Azhari Balqis Alrasyid Suyoto Borgo Mauly Nasution Budianto Panjaitan Cut Dahlia Iskandar Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Faisal Jamin Fakhrurrazi Fakhrurrarzi Fakhrurrazi Fakhrurrazi Feby Fema Amzani Nasution Hamdani Budiman Hamny Sofyan Husna Husna Ismail Ismail laras berlian xarolin M Daud AK M Jalaluddin M. Hasan M. Isa Mahdi Abral Mahdi Abrar Maryulia Dewi Muhammad Hambal Muhammad Jalaluddin Muhammad Zulfan Muttaqien Bakri Nurdin Hendri Nurjannah Annah NURLIANA NURLIANA Rastina Rastina Razali Daud Razali Razali Resti Reimena rina dwita Rinidar Rinidar Safika S, Safika Sagasias Ahmad Sandy Cakra Yuda Siti Hajar Siti Wardianah Matondang Sugito Sugito Sugito Sugito Teuku Reza Ferasyi Teuku Zahrial Helmi Ummu Balqis Usma Aulia Vara Tassa Sutari Waskito Waskito Wenny Novita Sari Winaruddin Winaruddin Yandi Syahputra

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