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STATUS ANTIOKSIDAN GLUTATION PADA PASIEN TUBERKULOSIS PARU DI BALAI KESEHATAN PARU (BKPM) MAKASSAR Yuniastuti, Ari; Yusuf, Irawan; Massi, Muh Nasrum; -, Budu
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.2745

Abstract

AbstrakAntioksidan merupakan senyawa untuk pertahanan tubuh host terhadap radikal bebas. Pada pasien tuberkulosis paru (TB paru), rendahnya antioksidan menyebabkan kerusakan jaringan. Salah satu antioksidan tubuh adalah glutation. Rendahnya glutation berhubungan dengan gangguan sistem imun, sehingga menyebabkan keparahan penyakit pada pasien TB paru. Penelitian ini bertujuan mengetahui kadar glutation plasma penderita tuberkulosis paru. Desain penelitian ini adalah kohort prospektif. Sampel TB paru dipilih di Balai Besar Kesehatan Paru Masyarakat (BBKPM), Makassar yang sesuai dengan kriteria inklusi dan eksklusi dan sesuai kadatangan (consecutive sampling). Dua mililiter darah diambil dari setiap sampel, kemudian dilakukan pemeriksaan kadar glutation dengan ELISA. Hasil penelitian menunjukkan bahwa kadar glutation sampel pasien TB paru sebelum pemberian obat antituberkulosis (OAT) sebesar 0,483830,0344 mM dan setelah pemberian OAT sebesar 0,494650,0371 mM. Setelah pemberian OAT kadar glutation mengalami peningkatan sebesar 2,2% meskipun masih lebih rendah dari kadar glutation normal.AbstractAntioxidants prevent hosts against free radicals. In pulmonary tuberculosis (TB) patients, the low level of antioxidants may cause tissue damage in oxidative hosts. One of antioxidants is glutathione. The poor glutathione level correlates with immune system disorder. The current study aimed to determine glutathione plasma levels in pulmonary tuberculosis patients. The study design was prospective cohort. Pulmonary TB samples were obtained from patients in the Lung Health Centre (BBKPM) in Makassar, and they were selected based on the inclusive and exclusive criteria and on their admittance times (a consecutive sampling technique). Two mililiters of blood was taken from each sample and the glutathione level was examined using ELISA. The study indicated that the glutathione level of the pulmonary TB patients before administered with antitubeculosis drug was 0.483830.0344 mM and after antituberculosis drug was 0.494650.0371 mM. After administration of antituberculosis drug the glutathione level increased by 2.2%, although still lower than normal glutathione level.
Effect of Photoprotective Cream of Mangosteen Pericarp Extract (Garcinia Mongostana L) Against 8-OHdG After UVB Exposure On Albino Mice Dian Amelia Abdi; Nasrum Massi; Khairuddin Djawad; Sri Vitayani; Nesyana Nurmadilla
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14705

Abstract

Objective: This study aims to determine the effect of the application of mangosteen pericarp extract cream(Garcinia Mangostana L) on levels of 8-OHdG on the skin of mice after exposure to UVB.Method: The study use a pure animal experimental research design with control group. The criteria forinclution were species Swiss albino mice, aged 6 – 9 weeks, weight 20-30 g, female gender and healthy.. Thesamples that met the criteria were divided into 5 groups : UVB, Base Cream plus UVB, 3 groups mangosteenpericarp extract cream with different concentration UVB radiation in mice using a 311 nm narrowband UVB(Dermalight 1000) with the power 450 mJ/cm three times a week for 4 week. Elisa examination was carriedout before and after the experiment.Result: This study used 25 mice albino selected randomly and were divided into 5 treatment groupconsisting of 5 group : UVB alone, UVB with base cream, UVB with mangosten pericarp extract cream withconcentration of 1 %, 3 % and 5 %. The Comperative test levels of 8-OhdG between each group with PostHoc test and found It was concluded that the decrease in OhdG was greatest in group 2 followed by groups5, 4, 3, and 1 (control group), respectively.Conclution: The ELISA levels of 8-OHdG was found that there are significant difference as between thevarious treatment groups (p>0,05). However, the increasing concentration of mangosten pericarp extractcream showed decreased level of 8-OHdG.
Deteksi Mikrobiota Plasenta Menggunakan PCR 16s rRNA Pada Persalinan Preterm Rajia; Muh. Nasrum Massi; Mardiana Ahamad; Sharvianty Arifuddin; Upik Anderiani Miskad
Poltekita : Jurnal Ilmu Kesehatan Vol. 15 No. 4 (2022): February
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v15i4.793

Abstract

Persalinan preterm adalah persalinan sebelum gestasi 37 minggu. Penelitian ini bertujuan untuk mendeteksi bakteri dalam plasenta yang paling sering muncul dan besar risiko terhadap kejadian persalinan preterm menggunakan PCR 16S rRNA. Desain penelitian adalah hybrid dengan desain cross sectional study dan case kontrol. Pengambilan sampel dilakukan dengan teknik purposive sampling pada ibu bersalin di Rumah Sakit Khusus Daerah Ibu dan Anak Siti Fatimah Makassar, Rumah Sakit Ibu dan Anak Siti Khadija I Makassar, dan Rumah Sakit Umum Daerah Kota Makassar pada Juni sampai Agustus 2019. Sampel penelitian terdiri dari 50 plasenta ibu bersalin, yang terdiri dari 25 ibu bersalin preterm (kasus) dan 25 ibu bersalin aterm (kontrol). Hasil analisa nucleotide BLAS, didapatkan bakteri Stenotrophomonas maltophilia (36,0%) merupakan bakteri modus tertinggi yang muncul pada plasenta preterm dan terdapat hubungan yang signifikan (p-value=0.039) terhadap kejadian persalinan preterm dengan nilai OR = 5.905 yang berarti ibu yang terinfeksi bakteri Stenotrophomonas maltophilia mempunyai risiko 5.905 kali untuk mengalami persalinan preterm, kemudian bakteri Acinetobacter sp. (12,0%) sedangkan modus tertinggi yang muncul pada plasenta aterm adalah bakteri Pseudomonas sp (21,7%) dan Bacillus (13%). Terdapat mikrobiota pada plasenta persalinan aterm maupun plasenta preterm, dan bakteri Stenotrophomonas maltophilia merupakan bakteri modus tertinggi yang muncul pada plasenta preterm dengan nilai OR = 5.905 yang berarti ibu yang terinfeksi bakteri Stenotrophomonas maltophilia mempunyai risiko 5.905 kali lipat untuk mengalami persalinan preterm
Perbandingan Metode Proporsi dengan Metode Resazurin Microtiter Assay (Rema) untuk Deteksi Mycobacterium tuberculosis yang Resisten Terhadap Rifampisin Yenti Purnamasari; Sartini Sartini; Faisal Attamimi; Muh. Nasrum Massi
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 2, No 2 (2015)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.576 KB) | DOI: 10.46496/medula.v2i2.2548

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Tuberculosis (TB) is one of the high infectious diseases in Indonesia caused by Mycobacteriumtuberculosis. Drugs which used for tuberculosis therapy has been widely reported experiencing resistance, while inappropriately treatment will lead to not effective and efficient care. Culture in Lowenstein Jensen medium is a gold standard to perform the drug susceptibility but it takes a long time to get results, therefore, new methods were developed to replace it, one of them is colorimetric method. This research aimed to compare Rifampicin susceptibility test in colorimetric method use REMA and culture method use Lowenstein Jensen medium. This study was a cross sectional with experimental laboratory design used 42 sputums from patients with tuberculosis, done at October 2010 until March 2011. In this research, 5 samples rifampicin resistant have been detected. As a results, we founded Rifampicin susceptibility test of REMA have 80 % sensitivity, 100 % specificity, 100 % PPV, and 97 % NPV when compared with culture method.Keywords:Proportion, Resazurin, Mycobacterium tuberculosis, Resistance
Do the acute lymphoblastic leukemia and non-hodgkin lymphoma patients have a worse prognosis of COVID-19 infection in children?: a case report Nadirah Rasyid Ridha; Bahrul Fikri; Rahmawaty Rahimi; Amiruddin Laompo; Conny Tanjung; Dasril Daud; Nasrum Massi
Health Science Journal of Indonesia Vol 12 No 1 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v12i1.4467

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Background: Coronavirus Disease 2019 (COVID-19) is a contagious disease caused by a new type of Coronavirus namely Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Children with tumors or autoimmune diseases are more susceptible, because of suppression of their immune system, chemotherapy, radiotherapy, or surgery on tumors. Case presentation: We present the clinical features 3 Acute Lymphoblastic leukemia and 1 Non-Hodgkin lymphoma patients who were infected with COVID-19 since July to August 2020 in our hospital. These were the first four cases identified as COVID-19 positive in Dr Wahidin Sudirohusodo Hospital. Case 1, 2, and 4 were diagnosed as moderate and common type of COVID-19, while case 3 was classified as severe type. They may be transmitted COVID-19 infection during hospitalization. All cases were recovered from COVID-19 after a combination therapy against virus, bacteria, and also respiratory support. Conclusion: Our case series of four pediatric cancer patients showed a good outcome after prompt treatment, suggesting that malignancy in children may not be a contributor factor for COVID-19 recovery. Keywords: COVID-19; acute lymphoblastic leukemia; non-hodgkin lymphoma; children Abstrak Latar belakang: Covid-19 (Coronavirus disease 2019) adalah penyakit menular yang disebabkan oleh Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Anak yang menderita kanker atau penyakit autoimun lebih rentan tertular karena penurunan system kekebalan tubuh, dampak kemoterapi, radioterapi, atau operasi tumor. Penyajian kasus: Kami melaporkan 3 pasien leukemia limfoblastik akut dan 1 pasien limfoma non-Hodgkin yang terinfeksi Covid-19 sejak Juli-Agustus 2020. Kasus tersebut adalah 4 kasus pertama yang teridentifikasi Covid-19 di Rumah Sakit Dr. Wahidin Sudirohusodo. Kasus 1,2, dan 4 terdiagnosis positif Covid -19 derajat sedang, sedangkan kasus 3 tergolong berat. Mereka kemungkinan terinfeksi Covid-19 saat perawatan. Semua kasus dinyatakan sembuh dari Covid-19 setelah pemberian obat anti virus, antibiotik, dan alat bantu pernapasan. Kesimpulan: Serial kasus dari 4 pasien kanker anak dengan outcome yang baik setelah pengobatan yang cepat mengindikasikan bahwa penyakit keganasan pada anak kemungkinan bukan faktor yang berkontribusi dalam kesembuhan Covid-19. Kata kunci: COVID-19; leukemia limfoblastik akut; limfoma non-hodgkin; anak
Risk Assessment in Hereditary Colorectal Cancer Family by Using APC and MSH2 mRNA Gene Expression and Bayesian Analysis Tjahjadi Robert Tedjasaputra; Mochammad Hatta; Muhammad Nasrum Massi; Rosdiana Natzir; Ilhamjaya Patellongi; Marcellus Simadibrata; Ibrahim Labeda; Rina Masadah; Muhammad Luthfi Parewangi; Prihantono Prihantono; Andi Asadul Islam; Agussalim Bukhari; Budu Budu; Rinda Nariswati
The Indonesian Biomedical Journal Vol 12, No 4 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i4.1329

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BACKGROUND: Some of colorectal cancers (CRCs) are familial, however, heterozygote relatives have approximately 80% lifetime risk of cancer. Risk assessment of CRC’s family could be calculated by direct measurement of mRNA gene expression and Bayesian theorem which is modifying initial background of pedigree risk with additional conditional information. This application has not been reported.METHODS: The cross-sectional translational sequential studies were performed: (1) adenomatous polyposis coli (APC) and MutS homolog (MSH)2 mRNA quantitative RT PCR gene expressions in tissue and whole blood CRC patients; (2) gene expression was determined in matched controls; and (3) pedigree and Bayesian analysis was calculated in the patient’s family of Proband.RESULTS: Fourty CRC and 31 control subjects were enrolled. The mean blood APC level control’s group was 13,261±670 fold-change (fc) and blood MSH2 level was 12,219±756 fc. The cut-off points for hereditary APC was 12,195 fc and MSH2 was 11,059 fc. The mean APC blood level in CRC subject was 11,578±2,638 fc and MSH2 blood level was 11,411±2,912 fc. There were significant differences APC and MSH level between tissue and blood level in CRC. Eight of 40 CRC subjects had a history of familial CRC. Four patients and 10 Probands were available for recurrence risk evaluation of pedigree analysis, RNA PCR quantitative and Bayesian calculation.CONCLUSION: There was determined a cut-off point of hereditary mRNA quantitative expression. The APC and MSH2 levels in CRC subjects were significantly lower than controls. Bayesian analysis allowed for the calculation of relative risk in CRC family members and considered in clinical practice.KEYWORDS: hereditary CRC, APC gene, MSH gene, Bayesian analysis
ISOLASI DAN IDENTIFIKASI GEN pneumcoccal surface adhesin A (psaA) SEBAGAI FAKTOR VIRULENSI Streptoccus pneumoniae Mustika Sari; Nikmatia Latief; Muh. Nasrum Massi
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 5 No. 1 (2020)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v5i1.8860

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Streptococcus pneumoniae (pneumokokus) merupakan flora normal pada traktus respiratorius atas yang juga dapat menjadi bakteri patogen penyebab invasive Pneumococcal Disease (IPD) seperti pneumoniae, otitis media, dan meningitis. Namun belum banyak yang melaporkan kejadian oleh bakteri ini pada penderita IPD khususnya di Kota Makassar. Belum ada metode gold standar dalam mengidentifikasi pneumokokus menjadi salah satu faktor sulitnya menegakkan diagnosis terkait dengan bakteri ini.  Maka dari itu tujuan dari penelitian ini untuk mengevaluasi kinerja metode pemeriksaan baik dengan menggunakan metode konvensioanl (bakteriologi kultur) dan metode molekuler dengan Polymerase Chain Reaction  (PCR) dalam mengisolasi dan identifikasi gen psaA sebagai faktor virulensi. Metode yang digunakan secara konvensional adalah  mikroskopis, kultur, dan uji biokimia dimana hasilnya mengarahkan pada spesies Streptococcus kemudin dilakukan uji susptibility dengan opthocin sedangkan untuk metode molekuler digunakan teknik PCR sebagai pembanding. Diperoleh hasil uji Opthocin 3 (12%)  sampel yang sensitive kemudian dilanjutkan dengan melakukan amplifikasi PCR gen PsaA menggunakan primer spesifik maka diperoelh juga pita dengan ukuran 834 bp yang menunjukkan bahwa pada ke-3 (100%) isolate S.pneumoniae terdeteksi  gen PsaA Kata kunci : Sputum, psaA, PCR, Streptococcus pneumoniae
DETECTION OF NONTUBERCULOUS MYCOBACTERIA (NTM) IN ISOLATES FROM SUSPECTED TUBERCULOSIS USING PCR IS6110 GENE Andi Nur Asrinawaty; Muh Nasrum Massi; Rizalinda Sjahril
Nusantara Medical Science Journal Volume I No. 1 Januari-Maret 2016
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v0i1.2203

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ABSTRACT  Introduction: NTM is a group of mycobacterium other than M.tuberculosis complex and M.Leprae, which found in the environment and can be pathogenic. Objectives: This study aim was to detect NTM isolates using PCR on target genes IS 6110. Methods: This study was conducted in Laboratory of Microbiology, Faculty of Medicine, Hasanuddin University and Laboratorium microbiology in Hasanuddin University Hospital. 14 sample isolates of NTM from sputum of suspected tuberculosis patients. The method using PCR IS6110 gene. Results: The results showed that of 14 samples, 8 positive samples with M. tuberculosis and 6 samples negative. Conclusions: IS6110 PCR can be used to detect NTM isolates from sputum of suspected tuberculosis patients. Keywords : NTM, PCR, IS6110, M.tuberculosis
THE ASSOCIATION OF POLYMORPHISM PROMOTER VEGF PROMOTER GENE POLYMORPHISM AND VEGF SERUM LEVEL WITH THE TYPE OF AGE-RELATED MACULAR DEGENERATION Andi M Ichsan; Habibah S Muhiddin; Nasrum Massi; Budu Budu
Nusantara Medical Science Journal Volume 1 No. 2 April - Juni 2016
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v1i2.2211

Abstract

ABSTRACT Introduction: One of the potential genetic links to Age-related macular degeneration (AMD) is vascular endothelial growth factor (VEGF). The aim of this study was to investigate the association of polymorphism promoter VEGF gene and VEGF serum level with the type of AMD. Methods: Genomic DNA was extracted from peripheral blood of 66 samples, consist of wet and dry AMD, and age-matched controls. DNA was subjected to polymerase chain reaction amplification at the position -460 coupled with the restriction fragment length polymorphism. VEGF serum level was measured by Enzyme-linked Immunosorbent Assay. Results: The polymorphism promoter VEGF gene demonstrated significant association with the type of AMD (p=0.004, OR=3.1 95% CI 1.1-8.6) and the risk for increasing VEGF serum level (p=0.011, OR=3.2 95% CI 1.18.9). Despite there was no significant association between dry and wet AMD with VEGF serum level (p=0.081), but this study revealed significant increased of VEGF serum level in AMD patients, compared to those in control’s serum (p<0.001). Finally, there was coassociation between polymorphism and VEGF level with the type of AMD (p<0.001). Conclusion: This study reveals the significant association between promoter VEGF gene polymorphism and VEGF serum level with the type of AMD of Indonesian patients.  Keywords: AMD, VEGF gene polymorphism; VEGF serum level.
THE IMPACT OF THE FREQUENCY OF 7,12- DIMETHYLBENZ [Á] ANTHRACENE (DMBA) ADMINISTRATION ON THE FORMATION OF DYSPLASIA AND SQUAMOUS CELL CARCINOMA ON THE SKIN OF ALBINO MICE Diana Muchsin; Khairuddin Djawad; Alwi A Mappiasse; Idham Jaya Ganda; Nasrum Massi; Gemini Alam
Nusantara Medical Science Journal Volume 1 No. 4 Oktober - Desember 2016
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v1i4.2248

Abstract

ABSTRACT Introduction: Dosage and frequency of 7,12-dimethylbenz[á] anthracene (DMBA)  com- pound exposure suspected to affect the rapid growth of squamous cell carcinoma (SCC) in albino mice. Objective: find out the impact of DMBA exposure to dysplasia and SCC formation on albino mice. Methods: Twenty-five albino mice were divided into five groups, namely: Group 1 is the control (acetone), group 2 is 5 albino mice given four times DMBA100 µg exposure for eight weeks, group 3 is 5 albino mice were given eight times DMBA100 µg exposure for eight weeks, group 4 is 5 albino mice were given twelve times DMBA100 µg exposure for eight weeks, and group 5 is 5 albino mice were given sixteen times DMBA 100 µg exposure for eight weeks. Histopathological examination were conducted to assess histopathological feature including inflammation, dysplasia, and SCC. Results: There is a significant effect of the frequency of DMBA exposure to the clinical figure and against histopathological feature of the skin albino mice.Conclusions:  Dosage and fre- quency of DMBA exposure affect the occurence of dysplasia and SCC, which are in- creasingly being DMBA exposure will accelerate the growth of SCC. The frequency of the most widely pose a SCC in DMBA 100 µg sixteen times exposure group, twice a week for eight weeks. Keywords: DMBA, dysplasia, squamous cell carcinoma
Co-Authors Adyatma Muhammad Nur Agus Sangka Pratama Ahyar Ahmad Alwi A Mappiasse Amiruddin Laompo Andi Alfian Zainuddin Andi Asadul Islam Andi M Ichsan Andi Nilawati Usman Andi Nur Asrinawaty Andi Zulkifli AS Anita Anita Ari Yuniastuti Asaad Maidin Asty Amalia Nurhadi Bahrul Fikri Budu - Budu Budu Bukhari, Agussalim Conny Tanjung Dasril Daud DAVID HANDOJO MULJONO Dewi Mutisari Dian Amelia Abdi Diana Muchsin Dwi Kesuma Sari Eddyman W Ferial Elly L. Sjattar Faisal Attamimi Faradillah Suhar Ferra Indrawati Firdaus Hamid Firdaus Kasim Gemini Alam Gemini Alam Habibah S Muhiddin Hendra Wibawa Herlina Rante Ibrahim Labeda Idham Jaya Ganda Ilhamjaya Patellongi Irawan Yusuf Irawati Djaharuddin Irma Andrianys Khairuddin Djawad Khairuddin Djawad Kusrini S. Kadar Latief, Nikmatia Linda Runtuwene Lokita Purnamasari M Natsir Djide Marcellus Simadibrata Mardiana Ahamad Mardiana Ahmad Mardiana Ahmad Mochammad Hatta Mochammad Hatta Muflihanah Muhammad Akmal A. Sukara Mustika Hutabarat Sari Mustika Sari Nadirah Rasyid Ridha Nasir Djide Nilawati Usman Norma Tiku Kambuno Nur Fajri Nurfadillah S Nurmadilla, Nesyana Parewangi, Muhammad Luthfi Prihantono Prihantono Rahmawaty Rahimi Rajia Ratna Rina Masadah Rinda Nariswati Ritha Pratiwi Rizalinda Sjahril Rizalinda Sjahril Rosana Agus Rosdiana Natzir Rusdianto Rusdianto Saidah Syamsuddin Sartini Sartini Sharvianty Arifuddin Sharvianty Arifuddin Shelly Salmah Solly Aryza Sri Vitayani Suanti Suhatman A. Hakim Supri Suryani As'ad Tjahjadi Robert Tedjasaputra UPIK ANDERIANI MISKAD, UPIK ANDERIANI Wardihan Sinrang Yenti Purnamasari