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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Journal of Aquaculture and Fish Health

Julie Ekasari

Department Of Aquaculture, Faculty Of Fisheries And Marine Science, IPB University, Bogor, West Java, Indonesia

Author-ID : 258464

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education

Published : 36 Documents Claim Missing Document

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Nursery Culture Performance of Litopenaeus vannamei with Probiotics Addition and Different C/N Ratio Under Laboratory Condition . WIDANARNI; DEBY YUNIASARI; . SUKENDA; JULIE EKASARI
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

Application of bioflocs technology and probiotics has improved water quality and production of Pacific white shrimp (Litopenaeus vannamei) culture. This experiment was to verify the effect of probiotic bacteria addition and different carbon:nitrogen (C:N) ratio on water quality and performance of Pacific white shrimp nursery culture. Nursery culture was carried out for 25 days in an aquarium under laboratory condition with stock density of one Post-Larvae (PL) (poslarval) per liter (24 PL/aquarium) of PL16 shrimp. Different C:N ratio resulted a significant difference on shrimp production performance. Treatment of 10 C:N ratio demonstrated the best shrimp growth (20.37 + 0.48% per day in weight and 6.05 + 0.41% per day in length), harvesting yield (1180 + 62 g/m3) and feed efficiency (121 + 6%). There was however no significant difference observed between treatments in water quality.

Primary Nutritional Content of Bio-Flocs Cultured with Different Organic Carbon Sources and Salinity JULIE EKASARI; ROSELIEN CRAB; WILLY VERSTRAETE
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

Application of bio-flocs technology (BFT) in aquaculture offers a solution to avoid environmental impact of high nutrient discharges and to reduce the use of artificial feed. In BFT, excess of nutrients in aquaculture systems are converted into microbial biomass, which can be consumed by the cultured animals as a food source. In this experiment, upconcentrated pond water obtained from the drum filter of a freshwater tilapia farm was used for bio-flocs reactors. Two carbon sources, sugar and glycerol, were used as the first variable, and two different levels of salinity, 0 and 30 ppt, were used as the second variable. Bio-flocs with glycerol as a carbon source had higher total n-6 PUFAs (19.1 + 2.1 and 22.3 + 8.6 mg/g DW at 0 and 30 ppt, respectively) than that of glucose (4.0 + 0.1 and 12.6 + 2.5 mg/g DW at 0 and 30 ppt). However, there was no effect of carbon source or salinity on crude protein, lipid, and total n-3 PUFAs contents of the bio-flocs.

Evaluation of Biofloc Technology Application on Water Quality and Production Performance of Red Tilapia Oreochromis sp. Cultured at Different Stocking Densities . WIDANARNI; JULIE EKASARI; SITI MARYAM
HAYATI Journal of Biosciences Vol. 19 No. 2 (2012): June 2012
Publisher : Bogor Agricultural University, Indonesia

This study evaluated the effect of biofloc technology (BFT) application on water quality and production performance of red tilapia Oreochromis sp. at different stocking densities. Three different fish densities were applied, i.e. 25, 50, and 100 fish/m3, and for each density there were Control (without external carbon input) and BFT treatments. Mixed sex red tilapia with an initial average body weight 77.89 + 3.71 g was cultured in 3 m3 concrete tanks for 14 weeks. Molasses was added on BFT treatments as the organic carbon source at a C/N ratio of 15. Control treatments of each density tested showed more fluctuated water quality parameters throughout the experimental period. The highest TAN and nitrite-nitrogen were observed in control treatment at a stocking density of 100 fish/m3 (3.97 mg TAN/L and 9.29 mg NO2-N/L, respectively). The highest total yield was observed in control treatment at the highest density treatment (43.50 kg), whereas the highest survival was obtained by BFT treatment at a density of 25 fish/m3 (97.78 + 0.77%). Total feed used in BFT was lower than that of control treatments in particular at 50 fish/m3 density (P < 0.05) suggesting that biofloc could be continuously harvested by the fish as other source of food.

Evaluation of Smoked Skipjack Processing Byproduct Meal as an Alternative Feed Ingredient for Juvenile Humpback Grouper Cromileptes altivelis Muhamad Agus Suprayudi; Fatma Hajiali; Nur Bambang Priyo Utomo; Julie Ekasari; Ichsan Fauzi
HAYATI Journal of Biosciences Vol. 23 No. 1 (2016): January 2016
Publisher : Bogor Agricultural University, Indonesia

This study aimed to evaluate the utilization of smoked skipjack processing byproduct meal (SPBM) to reduce the use of fish meal (FM) for juvenile humpback grouper Cromileptes altivelis. This study consisted of digestibility test of SPBM and biological test to observe growth performance. Five isonitrogenous and isocaloric experimental diets were used: Diet A contains 0% SPBM as a control diet, and diets B, C, D, and E contain 25%, 50%, 75%, and 100% of SPBM protein to substitute FM, respectively. Digestibility trial was performed for 14 days by adding Cr2O3 into the experimental diets and collecting fecal matter 40–60 minutes after each feeding. For growth trial, juvenile humpback grouper were kept in glass aquariums and fed by the experimental diet until apparent satiation for 60 days. Our result shows that the dry matter and protein SPBM apparent digestibility coefficient is lower compared to FM. The fish that was fed with diet B and C performed a comparable specific growth rate, feed efficiency, and protein retention compared to control (p > 0.05). However, lower specific growth rate, feeding efficiency, and protein retention were observed in fish that was fed with diet D (75% SPBM) and diet E (100% SPBM; p < 0.05). These results indicate that up to 50% of smoked SPBM can be used for the diet of humpback grouper.

Sex Reversal on Congo Tetra Fish (Micraleptus intterruptus ) Larvae Harton Arfah; , Alimuddin; K. Sumantadinata; Julie Ekasari
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTExperiment was performed to assess the effect of 17a-methyltestosterone (MT) treatment on Congo tetra fish larvae. To evaluate the optimal pattern of MT treatment, three different treatments were administrated. Three months old larvae were submerged in three different doses of MT; 1, 2 and 4 mg/l. These studies showed that the highest percentage of male fish was obtained by 4 mg/l MT treatment, 87,17%. The 2 mg/l and 1 mg/l MT treatments obtained 77,53% and 69,86% male respectively, two times higher than control, 38,96%. On the other hand, the 4 mg/l MT treatment also resulted the highest percentage of hermaphrodite fishes, 17,58%. The highest survival rate was shown by 1 mg/l MT treatment, 62,77% and the lowest was shown by the 4 mg/l MT treatment, 47,20%. The highest rate of fish length and weight was shown by the 4 mg/l MT treatment, 4,4 cm and 1,65 gram respectively. These findings suggest that MT treatment offers an advantage in growth of tetra Congo larvae. Key word : Sex reversal, methyltestosterone, Congo tetra fish, Micraleptus intterruptus. ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh perendaman larva di dalam larutan hormon 17a-metiltestosteron pada dosis 1, 2 dan 4 mg/l larutan. Persentase tertinggi ikan jantan dihasilkan oleh perlakuan 4 mg/l, yaitu 87,17%. Perlakuan 2 mg/l dan 1 mg/l masing-masing menghasilkan 77,53% dan 69,86% sedangkan kontrol menghasilkan 38,96% jantan. Efek lain dari perlakuan MT ini adalah hermafroditisme. Perlakuan 4 mg/l menghasilkan persentase hermafrodit tertinggi yaitu 17,58%, sedangkan pada kontrol kelangsungan hidup tertinggi diperoleh pada perlakuan 1 mg/l (62,77%) dan terendah pada perlakuan 4 mg/l (47,20%). Hasil tersebut menunjukkan adanya pengaruh dosis hormon terhadap kelangsungan hidup ikan. Pengukuran bobot dan panjang ikan pada setiap perlakuan menunjukkan nilai tertinggi dihasilkan oleh perlakuan 4 mg/l yaitu 1,65 gram dan 4,40 cm. Hal ini diduga bahwa hormon MT merangsang pula pertumbuhan ikan.Kata kunci : Pergantian kelamin, metiltestosteron, ikan tetra Kongo, Micraleptus intterruptus.

Bioflocs Technology: Theory and Application in Intensive Aquaculture System Julie Ekasari
Jurnal Akuakultur Indonesia Vol. 8 No. 2 (2009): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Bioflocs technology (BFT) is one of the developing technology in aquaculture which aimed to improve water quality and to enhance nutrient utilization efficiency. This technology is mainly based on the conversion of inorganic nitrogen in particular ammonia by heterotrophic bacteria into microbial biomass which further can be consumed by aquaculture organisms. The objective of this review is to discuss various aspect of BFT application in aquaculture including bioflocs formation process, technical requirement, bioflocs nutritional content and characterization techniques. Keywords: bioflocs, nitrogen, heterotrophic bacteria, ammonia, C/N ratio ABSTRAK Teknologi bioflok (BFT) merupakan salah satu teknologi yang saat ini sedang dikembangkan dalam akuakultur yang bertujuan untuk memperbaiki kualitas air dan meningkatkan efisiensi pemanfaatan nutrient. Teknologi ini didasarkan pada konversi nitrogen anorganik terutama ammonia oleh bakteri heterotrof menjadi biomassa mikroba yang kemudian dapat dikonsumsi oleh organisme budidaya. Tujuan penulisan makalah ini adalah untuk memaparkan berbagai aspek dalam BFT termasuk proses pembentukan bioflok, persyaratan teknis, kandungan nutrisi bioflok dan teknik karakterisasinya. Kata kunci: bioflok, nitrogen, bakteri heterotrof, ammonia, rasio C/N

Bacterial quorum sensing and the role of algae in bacterial diseases control in aquaculture . Wiyoto; Julie Ekasari
Jurnal Akuakultur Indonesia Vol. 9 No. 2 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Bacterial disease is one of the most common diseases in aquaculture practices which have a significant impact. Several researches noted that pathogenicity of a certain bacteria can be determined by its quorum sensing activity. Quorum sensing is a communication process of a certain bacteria with the same or different species of bacteria which involves the releasing and capturing of signal molecule to and from the environment. This activity will activate a certain target gene which further resulted in the expression of a phenotype by the bacteria. With regard to this characteristic, one of the methods to control bacterial diseases is by quorum sensing disruption. Several species of algae, both micro and macro, have been found to be able to intervense bacterial quorum sensing and thus can be used as an alternative in bacterial disease control. Key words: quorum sensing, bacterial disease, aquaculture, algae Abstrak Penyakit bakteri adalah salah satu penyakit yang paling umum dalam akuakultur dengan dampak yang cukup signifikan. Beberapa penelitian menunjukkan bahwa tingkat patogenitas suatu bakteri salah satunya ditentukan oleh aktivitas kuorum sensing bakteri. Kuorum sensing bakteri merupakan suatu proses komunikasi yang dilakukan oleh bakteri dengan bakteri lainnya baik yang sejenis maupun berlainan jenis yang berupa pelepasan dan penangkapan molekul sinyal menuju dan dari lingkungan sekitar bakteri tersebut. Aktivitas inilah yang akan menentukan ekspresi suatu gen target seperti patogenitas, sehingga salah satu metode yang dapat digunakan dalam mengendalikan penyakit yang disebabkan oleh bakteri adalah dengan mengganggu aktivitas kuorum sensing bakteri. Beberapa jenis alga, baik mikro maupun makro, diketahui dapat mengintervensi aktivitas kuorum sensing, dan dapat menjadi salah satu alternatif bagi pengendalian penyakit bakterial. Kata-kata kunci: kuorum sensing, penyakit bakterial, akuakultur, alga

Microbial abundance and diversity in water, and immune parameters of red tilapia reared in bioflocs system with different fish density (25 fish/m3, 50 fish/m3, and 100 fish/m3) Frid Agustinus; . Widanarni; Julie Ekasari
Jurnal Akuakultur Indonesia Vol. 9 No. 2 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThe objective of this experiment was to study microbial abundance and diversity in the water, and immune parameters of red tilapia Oreochromis sp. cultured in bioflok system with different fish stocking densities. The experiment comprised of two different factors, carbon source addition (bioflocs and control), and fish stocking density (25 fish/m3, 50 fish/m3, dan 100 fish/m3), with an experimental period of 99 days. Microbial load in water was determined biweekly, whereas immune parameters represented by fish blood profile were measured on day 0, 50, and 90. There was no significant difference in total bacteria count in the water of all treatments; there was however a tendency shown by all treatments that the microbial load in water increased along with the culture period. There were 4 genera of bacteria which particularly found in bioflok system, which are Acinetobacter sp., Corynobacterium sp., Listeria sp., dan Pseudomonas sp, and are suggested to play a role in bioflok formation. The percentage of phagocytic index of fish in bioflok system was higher than that in control, and may indicate that bioflok may stimulate the fish immune system.Keywords: bioflocs, red tilapia, bacteria, blood profile. ABSTRAKPenelitian ini bertujuan untuk mengidentifikasi kelimpahan dan keragaman jenis bakteri dalam air dan parameter imunitas ikan nila Oreochromis sp. yang dipelihara dalam sistem bioflok dengan kepadatan ikan yang berbeda. Penelitian terdiri atas dua faktor perlakuan yaitu penambahan sumber carbon (bioflok dan kontrol), dan padat penebaran ikan (25 ekor/m3, 50 ekor/m3, dan 100 ekor/m3) dengan lama waktu pemeliharaan ikan selama 99 hari. Kelimpahan bakteri diukur setiap 2 minggu sekali selama masa pemeliharaan. Parameter imunitas meliputi gambaran darah diukur dengan pengambilan contoh darah yang dilakukan pada tiga ekor ikan pada hari ke 0, 50, dan 99. Kelimpahan bakteri pada semua perlakuan pada setiap titik pengamatan tidak menunjukkan perbedaan yang nyata. Semua perlakuan menunjukkan kecenderungan peningkatan kelimpahan bakteri seiring dengan masa pemeliharaan. Terdapat 4 genus bakteri yang hanya ditemukan pada kolam bioflok yaitu Acinetobacter sp., Corynobacterium sp., Listeria sp., dan Pseudomonas sp yang diduga berperan dalam pembentukan bioflok. Persentase indeks fagositik pada ikan dengan perlakuan bioflok lebih tinggi dibanding kontrol, yang mengindikasikan peran bioflok sebagai stimulus sistem imun.Kata kunci: bioflok, nila merah, bakteri, gambaran darah.

Quality evaluation of fermented products of various local agroindustrials by-products: the effect on digestibility and growth performance of common carp juvenile Muhammad Agus Suprayudi; Gebbie Edriani; Julie Ekasari
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The aim of this research was evaluating the quality and digestibility of fermented local feedstuff as well as its effect on growth performance of common carp Cyprinus carpio juvenile. The local feedstuffs tested in this experiment were kapok seed, cassava peel, copra, rubber seed, and palm kernel meal. The previously milled feedstuff was fermented with instant yeast Saccharomyces cerevisiae with a dose of 0.9% w/w and incubated for 24 hours. Following this, the fermented feedstuffs were dried, mixed with reference diet with a ratio of 3:7 and supplemented with 0.5% Cr2O3 as digestibility test indicator. Common carp juveniles with an initial average body weight of 14,11±1,28 g were cultured for 30 days. Fish feces were collected since the 6th day of culture, and followed by laboratory analyses. The results show that fermentation could increase the crude protein content of feedstuff with a range of 16.85‒31.11%, and decrease crude fiber with a range of 2.45‒31.65% with the exception of copra. Furthermore, fermentation also increased the feed digestibility including protein, energy, and total digestibility by the tested fish, as it is shown that the use of fermented feedstuffs may increase protein digestibility 3.88‒11.73%, 2,21‒10,24%, and 3,63‒72,37%. Finally, it can be concluded that fermentation can increase the digestibility of feed with local ingredients by common carp juvenile. Keywords: fermentation, digestibility, local materials, common carp

Improvement of cocoa-pod husk using sheep rumen liquor for tilapia diet Dedi Jusadi; Julie Ekasari; Azis Kurniansyah
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Two experiments were conducted to evaluate the effect of sheep rumen liquor enzyme addition on the reduction of cocoa-pod husk meal (CPHM) fiber content, and the digestibility of hydrolyzed CPHM for tilapia Oreochromis niloticus. In the first trial, sheep rumen liquor enzyme was added with various concentration, i.e. 0, 50, 100 and 150 mL/kg CPHM with three different incubation periods, namely 0, 12, and 24 hours. In the second trial, digestibility was determined by the addition of Cr2O3 as the indicator in both reference and experimental diets, i.e. feed with hydrolyzed CPHM and unhydrolyzed CPHM. Tilapia with an average body weight of 3.86±0.44 g were stocked at a density of 15 fish/aquarium and were maintained for 15 days. In the first trial, CPHM hydrolyzed with 150 mL/kg and incubated for 12 and 24 hour showed the lowest crude fiber content (21.38% and 21.67%). Apparent digestibility coefficient of hydrolyzed CPHM was 33.95%, which was higher than unhydrolyzed CPHM (10.97%). As conclusions sheep rumen liquor enzyme addition was effective to decrease crude fiber content of CPHM and improve the apparent digestibility coefficient of CPHM for tilapia diet. Keywords: sheep rumen liquor enzyme, cocoa-pod husk meal, digestibility, tilapia ABSTRAK Dua tahap penelitian dilakukan untuk mengevaluasi penambahan enzim cairan rumen domba dalam menurunkan kandungan serat kasar kulit buah kakao (KBK) dan mengevaluasi ketercernaan KBK yang telah dihidrolisis dengan enzim cairan rumen domba dalam pakan ikan nila Oreochromis niloticus. Pada penelitian tahap satu, enzim cairan rumen domba ditambahkan dengan konsentrasi yang berbeda yaitu 0, 50, 100, dan 150 mL/kg KBK dengan lama inkubasi yang berbeda yaitu 0, 12, dan 24 jam. Pada penelitian tahap dua, nilai ketercernaan ditentukan dengan menggunakan indikator Cr2O3 yang ditambahkan ke dalam pakan acuan dan pakan perlakuan, yaitu pakan dengan penambahan KBK yang telah dihidrolisis dengan dosis terbaik pada penelitian tahap satu (KBKe) dan kulit buah kakao tanpa hidrolisis (KBK). Ikan nila yang digunakan mempunyai bobot rata-rata 3,86±0,44 g ditebar dengan kepadatan 15 ekor/akuarium dan dipelihara selama 15 hari. Hasil penelitian tahap satu menunjukkan hidrolisis KBK dengan menggunakan cairan rumen 150 mL/kg dan lama waktu inkubasi 12 jam dan 24 jam mempunyai nilai serat kasar KBK terendah yaitu sebesar 21,38% dan 21,67%. Pada uji ketercernaan terlihat bahwa nilai ketercernaan bahan KBKe lebih tinggi (33,95%) dibandingkan dengan KBK (10,97%). Dengan demikian dapat disimpulkan bahwa penambahan enzim cairan rumen domba dapat menurunkan kandungan serat kasar kulit buah kakao dan meningkatkan ketercernaan kulit buah kakao pada pakan ikan nila. Kata kunci: enzim cairan rumen domba, kulit buah kakao, ketercernaan, ikan nila

Co-Authors , Alimuddin , Sarmin . Alimuddin . SUKENDA . Wiyoto Abidin Nur Anang Fajrin Andri Hendriana Apriana Vinasyiam Apriana Vinasyiam, Apriana Arif Dwi Cahyo Azis Kurniansyah Billi Rifa Kusumah Christian Ernsz Pattipeilohy DEBY YUNIASARI DEDI JUSADI Dian Hardiantho Dodi Hermawan Dwi Hany Yanti Eddy Supriyono Enang Harris Surawidjaja Farida Fitriani Fatma Hajiali Fauzan Frid Agustinus Gebbie Edriani Giri Maruto Darmawangsa Glenaldi Halim Hanif Azhara, Muhammad Harton Arfah Hasan Nasrullah Hilda Kemala Pasha Hilmi Fauzi I Kadek Sumiana Ichsan Achmad Fauzi Idul La Muhamad Indi Jaka Nugraha Ing Mokoginta Iwan Sumantri Jhon Lamhot F. Napitupulu Jibal Rizki Akbar Arzi K. Sumantadinata Kukuh Nirmala M. Zairin Junior Mia Setiawati MUHAMMAD AGUS SUPRAYUDI Muhammad Arif Mulya Muhammad Ilham Labulal Banin Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur Bambang Priyoutomo Nurbambang Priyo Utomo Nurfadhilah . Nurul Mashita Putri Elas Ratih Kemala Dewi Reza Karunia Senja Ridwan Siskandar Ridwan Siskandar ROSELIEN CRAB Salsabila Setya Aulia Samsu Adi Rahman SITI MARYAM Sri Nuryati Sujaka Nugraha Sukenda Sumitro Syarifah Ruchyani Tangkas Mahendra Tatag Budiardi Taufik Shidik Adi Nugroho Shidik Wellya Wichi Meritha Wida Lesmanawati Widanarni Widanarni Widya Puspitasari WILLY VERSTRAETE Wiyoto Wiyoto Yanti Inneke Nababan

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