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All Journal Journal of Natural Sciences and Mathematics Research BIOMA THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST PEDAGO BIOLOGI AL-HAYAT: Journal of Biology and Applied Biology Journal of halal product and research (JHPR) Borneo Journal of Medical Laboratory Technology Humanism : Jurnal Pengabdian Masyarakat International Journal of Advanced Health Science and Technology Empowerment: Jurnal Pengabdian Masyarakat Prosiding Seminar Nasional Pengabdian Kepada Masyarakat
Fitrotin Azizah
Universitas Muhammadiyah Surabaya
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Pemeriksaan Jumlah Kapang Sebagai Indikator Kualitas Terasi Di Pasar Tambaksari Surabaya Dita Artanti; Fitrotin Azizah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.572 KB) | DOI: 10.30651/jmlt.v2i2.3387

Abstract

The main problem that occurs in the community is the lack of awareness of the importance of hygiene during the manufacture and storage of shrimp paste. This can trigger one of the contaminations originating from microorganisms including mold. The aimed of this study was to determine the number of molds as an indicator of shrimp paste quality in the Tambaksari market in Surabaya. This research is descriptive. The population of this research is shrimp paste traders in Tambaksari market in Surabaya. The research variable is the number of molds in the shrimp paste in the packaging without a brand in the Tambaksari market in Surabaya. Data on the number of molds was tabulated and analyzed descriptively by calculating the percentage of samples that were of good quality and not good. The results of the research on the number of mold colonies on shrimp paste sold in the Tambaksari market in Surabaya showed that the number of mold colonies in shrimp paste samples was safe to consumed because the amount was <2 x 102 colonies / gr, the average percentage of all shrimp paste samples was 100% good quality. The highest growth rate of mold colonies was 19 x 101 colonies / gr and the lowest growth rate of mold colonies was 0 x 101 colony / gr. Keywords : The Number of  Mold, Shrimp paste
GAMBARAN PEMERIKSAAN KADAR GLUKOSA DAN KOLESTEROL PADA LANSIA Nur Vita Purwaningsih; Fitrotin Azizah; Ellies Tunjung Sari Maulidiyanti; Dita Artanti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v2i2.10872

Abstract

The increasing life expectancy based on WHO indicates an increase in the number of elderly people (elderly) so that there is an increase in health problems in the elderly due to the aging process which causes many changes in the body of the elderly. One of the changes in the body of the elderly is the blood glucose and cholesterol regulation system, resulting in an increase of more than normal. The purpose of this study was to determine the profile of glucose and cholesterol levels in the elderly. This research is observational. The population and sample in this study were the elderly in Karang Penang. The examination method used in this examination is the POCT (Point Of Care Test) examination method. The results showed that the elderly who had levels as much as 10 respondents (20%) samples had normal glucose levels, while as many as 40 respondents (80%) samples had high glucose. For cholesterol levels, 20 respondents (40%) samples had normal cholesterol levels, while 13 respondents (26%) samples had threshold cholesterol levels, then 7 respondents (14%) samples had high cholesterol levels. Keywords        : blood glucose, cholesterol and the elderly
Uji Efektifitas Perasan Jahe (Zingiber officinale) Terhadap Pertumbuhan Jamur Candida albicans Fitrotin Azizah; Dita Artanti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.11282

Abstract

Candidiasis is one of the most common infectious diseases caused by Candida sp. In 2017 the Mycology Division of Dr. Soetomo said there were 67 patients diagnosed with candidiasis. The use of natural ingredients as an alternative to traditional medicine is increasing because it is safe for consumption and does not cause side effects. One of the natural ingredients is Ginger (Zingiber officinale). The choice of using ginger juice (Zingiber officinale) is because the ingredients are easy to obtain and easy to apply by the community. This study aims to determine the antifungal effectiveness of ginger (Zingiber officinale) juice against the growth of Candida albicans. This research is experimental by doing the treatment which was repeated 4 times, namely the concentration of ginger juice 20%, 40%, 60%, 80%, 100%, negative control (aquadest) and positive control (ketoconazole). The inhibition zone examination method used the Kirby Bauer diffusion method which was characterized by the formation of a clear zone around the paper disc on MHA (Muller Hinton Agar) media. The inhibition zone formed was measured using a caliper, then the data obtained were analyzed using the Mann-Whitney test. From laboratory examination, it was found that the average diameter of inhibition formed with concentrations of 20%, 40%, 60%, 80%, and 100% was 0 mm, and the negative control (aquadest) was 0 mm. The results of statistical analysis showed that 0.05 obtained a significant value between the administration of juice concentrations of 20%, 40%, 60%, 80%, 100% and not given (negative control-aquadest) = 1,000. From these results, it was concluded that there was no significant difference between the concentrated juice of 20%, 40%, 60%, 80%, 100% and not given (negative control - distilled water)Keywords : Ginger (Zingiber officinale), Candida albicans
Pengaruh Perasan Daun Belimbing Wuluh (Averrhoa bilimbi) Terhadap Pertumbuhan Bakteri Escherichia coli Patogen Fitrotin Azizah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.529 KB) | DOI: 10.30651/jmlt.v1i1.982

Abstract

 abstract (Averrhoa bilimbi) is one of the plants that can be used as an antibacterial, good flowers, stems, leaves and stems have benefits and efficacy. Chemical constituents of the leaves starfruit are tannins, flavonoids, saponins. The active ingredient in the leaves starfruit is tannin. Escherichia coli is a bacterium that causes diarrhea. From the above discussion, the authors raised the theme of Influence starfruit juice of the leaves on the growth of pathogenic E. coli bacteria. Formulation of the problem researchers is whether there is influence starfruit juice of the leaves on the growth of Escherichia coli pathogens. This study aims to determine the concentration that could inhibit and kill Esherichia coli Escherichia coli. This research is experimental. The sample used is leaf green starfruit not so young in a fresh state taken in the area around the boarding author Sutorejo 11B stay. In this study, the sample size for each treatment as much as 3 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10% and C (control). Independent variables are starfruit juice of the leaves, while the dependent variable growth of Escherichia coli. When the study carried out in January and July 2012. Data on the effect of starfruit juice of the leaves on the growth of Escherichia coli tested by laboratory examination and data collection techniques using Chi-Square 0:05. Based on the results it appears that at a concentration of 100% and 90% were able to kill the bacteria Escherichia coli, whereas the inhibitory power ranging from a concentration of 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%. From Chi-Square test was obtained λ2 count <of λ2 table. From this it turns out there leaves starfruit juice effect on the growth of Escherichia coli pathogenic bacteria.Keyword : Leaves starfruit, E. Coli
Test the effectiveness of Breadfruit (Artocarpus altilis) leaf extract on the growth of Staphylococcus aureus Fitrotin Azizah; Anindita Riesti Retno Arimurti; Dita Artanti; Yeti Eka Sispita Sari
Journal of Natural Sciences and Mathematics Research Vol 6, No 1 (2020): June
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.2020.6.1.11151

Abstract

Infectious diseases caused by Staphylococcus aureus are a health problem in Indonesia. Antibiotics can be used as the treatment, but currently Staphylococcus aureus is resistant to antibiotics. Therefore, it is necessary to find alternative medicine. One of the plants that has characteristics to be used as herbal medicine is Breadfruit leaves. The purpose of this study was to determine the effect of concentration on breadfruit leaf juice which can inhibit the growth of Staphylococcus aureus bacteria. This type of research is experimental. The study population was pure Staphylococcus aureus bacteria on NAS (Nutrient Agar Slant) media. The sample in this study was pure Staphylococcus aureus transferred from pure culture media (NAS), while the number of repetitions of the sample was 5 times the treatment, in this study were P1 (100%), P2 (75%), P3 (50%), P4 (25%), P5(0%). Data on the growth of Staphylococcus aureus bacteria was obtained by indirect observation, namely through laboratory tests and data collection techniques using the ANOVA test with an error rate (0.05) and followed by the Tukkey HSD test through the SPSS16.0 program. Based on the results of the antibacterial test of breadfruit leaf juice (Artocarpus altilis) in the ANOVA test, the probability number was 0.000 and there was an effect on the growth of Staphylococcus aureus bacteria. Followed by the Tukkey HSD test, it was obtained at a concentration of 25% which is the minimum inhibitory power, the effective concentration to inhibit the growth of Staphylococcus aureus at a concentration of 75%. From the data above, it can be concluded that there is an effect of breadfruit leaf juice (Artocarpus altilis) on the growth of Staphylococcus aureus.©2020 JNSMR UIN Walisongo. All rights reserved. 
The quality of Haemoglobin examination results with variations in incubation time using the Cyanmethemoglobin method Ellies Tunjung; Fitrotin Azizah; Vella Rohmayani; Baterun Kunsah; Rizka Vera Yunia
Journal of Natural Sciences and Mathematics Research Vol 6, No 2 (2020): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.2020.6.2.11224

Abstract

Anaemia is a condition where blood red blood levels or haemoglobin is lower than normal values. More than 50% of cases of anaemia are spread throughout the world. One of checks to diagnose anaemia is examination of haemoglobin levels. Examination of haemoglobin levels can be determined by several methods, namely the Sahli’s method, the Cyanmethemoglobin method manually and by automatic method. Examination of haemoglobin levels with a of more than 5 minutes incubation time leads to inaccurate results. The purpose of this research is to determine the effect of incubation time on the results of haemoglobin level examination of the Cyanmethemoglobin method. This type of research is experimental research to determine the effect of the incubation time on the results of haemoglobin level examination of the Cyanmethemoglobin method. The population in this research is outpatients with a diagnosis of kidney failure at Gatoel Hospital, Mojokerto, in March 2019. The number of examination samples is 9 samples which is carried out at the Gatoel Hospital Laboratory, Mojokerto. The results of this research show that the average haemoglobin incubation rate of 5 minutes is 7,52 g/dl, incubation of 20 minutes is 7,18 g / dl, and incubation of 30 minutes is 7,04 g / dl. The conclusion of this research is that there is no effect on the delay in incubation time on the results of the haemoglobin level examination of the Cyanmethemoglobin method.
Pemeriksaan Cemaran Kapang Pada Udara Dan Pakaian Santri Di Salah Satu Pondok Pesantren Surabaya Timur Anindita Riesti Retno Arimurti; Fitrotin Azizah
Al-Hayat: Journal of Biology and Applied Biology Vol 4, No 1 (2021)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v4i1.7062

Abstract

Dormitory is a place for student who built in various small scales to a very large scale. Of the many dormitory residents with bad space such as lack of ventilation and incoming sunlight, the large number of scattered students' clothes causes the room to become damp. The presence of air mold is caused by the lack of ventilation as well as the humidity of the room environment in the dormitory which can cause several diseases, one of which is ARI. The purpose of this study was to determine whether or not there is mold contamination on air in the room and clothes of the students at one of the Islamic boarding schools in East Surabaya. This type of research is descriptive. The sample used is air in several dormitory rooms at one of the Islamic boarding schools in East Surabaya. This research method uses the isolation technique of Sabauraud Dextose Agar (SDA) media which is partially opened and then placed in several rooms in this Islamic boarding school. Second, 30 samples of clothes were taken randomly from students from different disciplines and then scratched on SDA media. In the results of macroscopic observations, of the 30 sampled rooms. Microscopic colonies of cotton taken from the media showed positive results for hyphae, mycellium, spores, and conidiospores. The results obtained were positive there were Aspergillus sp.  with the percentage was 100% while the results of the students' clothing swabs, from 30 clothing samples found 80% positive for mold contamination.
Effect of Aloe vera extract for reducing formaldehyde level in tuna fish for halal and thoyyib food Subhan Rullyansyah; Fitrotin Azizah; Baterun Kunsah
Journal of halal product and research (JPHR) Vol. 3 No. 1 (2020): Journal of Halal Product and Research (JHPR)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jhpr.vol.3-issue.1.20-24

Abstract

This study aims to determine the effect of Aloe vera extract in reducing formaldehyde levels in tuna processed into "pindang" after being given Aloe vera extract. This type of research is experimental. The population in this study was boiled fish sold in the Sawotratap Market. This research was conducted at the Laboratory of Chemistry at the University of Muhammadiyah. Data were analyzed using analysis of variance at a significant level α = 0.05 followed by the Duncan test. The average formaldehyde concentration before being given Aloe vera extract at a concentration of 0% is equal to 23.7 mg / L, and the lowest formalin content in boiled fish with 100% treatment is equal to 5.6 mg / L. Aloe vera extract showed a significant decrease in formaldehyde levels in boiled fish (p <0.05). It can be concluded that the administration of Aloe vera extract is effective in reducing formaldehyde levels.
Kontaminasi Kapang pada Ikan Pindang yang Dijual di Pasar Tradisional Mangga Dua Kota Surabaya Ruspeni Daesusi; Anindita Riest Retno Arimurti; Asy'ari Asy'ari; Fitrotin Azizah; Vera Selviana
PEDAGO BIOLOGI Vol 10 No 1 (2022)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/pb:jppb.v10i1.14445

Abstract

Ikan pindang umumnya mudah busuk dan rusak karena mempunyai kadar air yang relatif tinggi dan sesuai dengan pertumbuhan mikroorganisme, terutama bakteri pembentuk lendir dan kapang. Penelitian ini bertujuan untuk mengetahui kontaminasi kapang dan jenis kapang pada ikan pindang yang dijual di Pasar Mangga Dua Surabaya. Jenis penelitian adalah deskriptif. Populasi penelitian adalah ikan pindang yang dijual di Pasar Mangga Dua Surabaya. Total sampel keseluruhan sebanyak 30 ikan pindang. Pemeriksaan jenis kapang dilakukan dengan metode isolasi kapang dari sampel ikan pindang. Kemudian dilanjutkan mengkulturkan pada media SDA (Saboround Dextrose Agar) dan diinkubasi selama 5-7 hari pada suhu ruang. Hasil penelitian menunjukan bahwa 8 sampel (27%) terkontaminasi dan 22 sampel (73%) tidak tekontaminasi kapang. Pada ikan pindang ditemukan jenis kapang Aspergillus sp.
Uji Antibakteri Perasan Daun Kersen (Muntingia Calabura L) Dalam Berbagai Konsentrasi Terhadap Pertumbuhan Escherichia Coli Secara Invitro Fitrotin Azizah; Lina Listiana; Mulya Fitrah Juniawan; Yatimatus Sholihah
PEDAGO BIOLOGI Vol 10 No 1 (2022)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/pb:jppb.v10i1.14440

Abstract

Penyakit infeksi merupakan penyakit yang sangat populer di Indonesia sejak lalu, diantaranya infeksi usus (diare). Beberapa bakteri yang dapat menyebabkan diare yaitu Escherichia coli, Vibrio cholera, Shigella sp dan Campylobacter. Pengobatan diare bisa dilakukan dengan pemberian obat-obat kimia dan obat-obat tradisional. Pilihan bahan yang mungkin dapat digunakan sebagai alternatif antibakteri adalah tanaman kersen (Muntingia Calabura L). Penelitian sebelumnya menunjukkan bahwa ekstrak dari daun kersen (Muntingia Calabura L) mampu menghambat pertumbuhan bakteri karena kandungan yang terdapat di dalamnya seperti flavonoid, tanin dan saponin. Sedangkan penelitian ini menggunakan perasan. Penelitian berikut ini bertujuan untuk mengetahui pengaruh perasan daun kersen (Muntingia Calabura L) terhadap pertumbuhan Escherichia coli. Jenis penelitian ini adalah eksperimental. Populasi dalam penelitian ini adalah bakteri Escherichia coli yang diambil dari biakan murni Escherichia coli yang ditanam di media NAS(Nutrient agar slant), dan yang digunakan sebagai sampel adalah bakteri Escherichia coli pada masing-masing konsentrasi yang dilihat pertumbuhannya pada media EMB setelah diinkubasi selama 24 jam. Jumlah pengulangan sampelnya sebanyak 3 kali dan 11 perlakuan yaitu 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10% dan 0% (sebagai kontrol). Data pertumbuhan bakteri Escherichia coli diperoleh melalui uji laboratorium. Analisa data statistik menggunakan uji Chi-square dengan taraf signifikan 0,05 untuk menentukan ada tidaknya pengaruh perasan daun kersen (Muntingia calabura L) terhadap pertumbuhan bakteri Escherichia coli. Hasil penelitian menunjukkan bahwa pada konsentrasi 100% dan 90% mampu menghambat pertumbuhan bakteri Escherichia coli, dan sedangkan pada konsentrasi 80% kebawah tidak mampu menghambat pertumbuhan bakteri Escherichia coli.
Co-Authors Abu Shofian Saifullah Aini Ahmad Anindita Riest Retno Arimurti Anindita Riesti Retno Arimurti Asy'ari Asy'ari Baterun Kunsah Dellanis Arina Putri Dita Artanti Ellies Tunjung Sari Maulidiyanti Eni Sumarliyah Firdausi Nuzula Lina Listiana Mulya Fitrah Juniawan Nur Vita Purwaningsih, Nur Vita Rahma Widyastuti Rifqi Athawirya Rinza Rahmawati Samsudin Rizka Vera Yunia Ruspeni Daesusi Ruspeni Daesusi Salwa Sausan Salsabila Siti Mardiyah Siti Mardiyah Subhan Rullyansyah Sudirman Sudirman Tri Ade Saputro Vella Rohmayani Vera Selviana Yatimatus Sholihah Yeti Eka Puspita Sari Yeti Eka Sispita Sari