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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia VALENSI Current Biochemistry Journal Indonesian Journal of Pharmaceutical Science and Technology Justek : Jurnal Sains Dan Teknologi Jurnal Farmamedika (Pharmamedica Journal)
Djarot Sasongko Hami Seno
Department Of Biochemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Immunology & microbiology Mathematics Medicine & Pharmacology

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Stabilitas Laju Fotosintesis dan Produktivitas Rimpang Genotipe Curcuma aeruginosa dan Curcuma zanthorrhiza Djarot Sasongko Hami Seno; Mohamad Rafi; Maria Bintang; Popy Asri Kurniatin; Waras Nurcholis
Indonesian Journal of Agronomy Vol. 48 No. 1 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.533 KB) | DOI: 10.24831/jai.v48i1.30100

Abstract

Curcuma aeruginosa Roxb. rhizome is an essential herb material with significant antimicrobial, anticancer, and antioxidant activities. Thus, it is important to search for C. aeruginosa plant that has the rhizome yield trait with more excellent stability in changing environmental situations. This research aimed to evaluate the genotype x environment interaction using additive main effects and multiple interaction (AMMI) analysis for photosynthetic rate and rhizome yield of C. aeruginosa genotypes. Twenty clones of C. aeruginosa plus three control varieties of C. zanthorrhiza Roxb. were evaluated in three environments (Bogor, Cianjur, and Sukabumi) using a randomized complete block design with three replicates. The photosynthetic rate and rhizome yield showed significantly (p < 0.01) genotype x environment interaction. The AMMI2 explained 100% variability of the photosynthetic rate and rhizome yield parameters. G2, G10, G12, G13, G14, G16, G17, G19, and G20 were stable genotypes for rhizome yield based on AMMI analyses. Meanwhile, the stable genotype in photosynthetic rate recorded in G4, G5, G6, G8, G11, G13, and G14. Keywords: AMMI, genotypes, multilocation trials, pink and blue ginger, varieties
Identification of Bioactive Compounds and α-Glucosidase Inhibition Activity of Caesalpinia bonduc Seed Extract In vitro Dwitasari, Oczhinvia; Hami Seno, Djarot Sasongko; Safithri, Mega
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

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Abstract

This study is aimed for finding alternative ways to treat diabetes. Many studies have beendone before, but focused on identification of α-glucosidase inhibition mechanism. This study wasconducted by extracting Caesalpinia bonduc seed using two methods maceration by ethanol 96%and the others boil by water. The ethanol extract was then fractioned into three fractions, n-hexane,ethyl acetate, and ethanol. To find the bioactive compounds, the ethanol extract was then tested byseveral phytochemistry tests. The phytochemistry test showed positive results for alkaloid, flavonoid,saponin and triterpenoid. The inhibition mechanism was tested using pNPG. The inhibition testshown that the mechanism was a competitive inhibition, by the Dixon plot. The calculated inhibitionconcentrations for 50% substrate (IC50) for ethyl acetate fraction are 1655.8079 μg/ml for 5 mMsubstrate and 803.9521 μg/ml for 10 mM substrate. From the results of this study, we concluded thatthe extract of Caesalpinia bonduc has inhibition activity toward α-glucosidase enzyme. Keywords: α-glucosidase inhibition, Caesalpinia bonduc seed extract, diabetes treatment
Transfer Gen Badh2 Termutasi Varietas Aromatik Mentik Wangi ke Varietas Nonaromatik Ciherang Djarot Sasongko Hami Seno; Bambang Padmadi; Dewi Praptiwi; . Sugihartati; . Taufiq; Muhammad Taufan Fatahajudin; Helmy Ramadhan Al Anshary; Tri Joko Santoso; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Replacement of non-fragrant-native badh2 gene with mutated badh2 of fragrant rice is an alternative to engineer new fragrant rice varieties with good agronomic traits as those of non-fragrant. Fragrant gene (mutated badh2) of Mentik wangi donor was introgressed into non-fragrant Ciherang host through site-directed crossing. Mentik Wangi was crossed with Ciherang, and the progeny was further backcross until BC3F1. Bradbury marka-assisted PCR was used to select progeny in every cross and backcross generation. Ciherang, Mentik Wangi, and their cross/backcross showed different PCR profiles. The statues of badh2 gene (native/mutated), as well as alleles (homozygote/heterozygote) between samples were identified. Mutated badh2-introgression was also observed within the selected heterozygote cross or backcross progenies (Fl, BCl, BC2, and BC3), indicated successful transfer of mutated badh2 gene from donor to host.
Aplikasi Berbagai Marka Aromatik pada Varietas Padi Indonesia Djarot Sasongko Hami Seno; Satya Nugroho; Tri Joko Santoso; Dimas Adrianto; Dewi Praptiwi; Aniversari Apriana; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 3 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

This research applied various badh2.7 and badh2.2 fragrant markers (Bradbury et al., 2005b, Lang and Buu 2008, Shi et al., 2008, Sakthivel et al., 2009) on popular Indonesia non-fragrant (Ciherang, Fatmawati) and fragrant (Pandan wangi, Rojo Lele, Mentik Wangi, Gunung Perak, Pulu mandoti, Pare Kembang, Sintanur) rice varieties. For comparison, IR64, Nippon bare and Taipei 309 were included. Rice DNA samples were isolated from young  leaves, and PCR amplified using each of those fragrant markers. Results using all badh2.7 markers were consistently supported the existence of 2 group badh2.7 mutation pattern, while the use of badh2.2 marker indicated that there was no ex on 2 mutation. Badh2.7 sequence analysis of non-fragrant Ciherang, and aromatik member of group 1 (Pandan Wangi), as well as group 2 (Mentik Wangi) showed different mutation pattern. 
Identifikasi gen aroma pada progeni-progeni backcross antara varietas Ciherang dengan Pandan Wangi Djarot Sasongko Hami Seno; Akhmad Endang Zainal Hasan; Tri Joko Santoso; Bram Kusbiantoro; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 2 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Marker-assisted PCR has been considered as the most potential method for fragrant selection. RM223 is the only suitable marker to identify mutated badh2 gene of Pandan Wangi. This research applies RM223-assisted PCR in the introgression of fragrant gene (mutated badh2) of Pandan Wangi variety, to engineer non-transgenic fragrant variety with good agronomic traits as those of Ciherang.  Gene introduction was carried out through site-directed crossing; Pandan Wangi was crossed and backcrossed to Ciherang until heterozygot BC5F1, followed by selfing to obtain homozygot BC5F2.  RM223-assisted selection was conducted in each cross and backcross generation. RM223 was able to identify native, mutated and heterozygot badh2 of Ciherang, Pandan Wangi, and their cross/backcross progenies, respectively. Therefore, the introgression of mutated badh2 within progenies were observed, as well as the statues of badh2 gene (native/mutated) and alleles (homozygot/heterozygot). Further backcross and selfing to obtain BC5F2 is in progress.
Characteristics of Glucose Oxidase Gene (GGOx) from Aspergillus niger IPBCC 08.610 Popi Asri Kurniatin; Laksmi Ambarsari; Annisa Dhiya Athiyyah Khanza; Inda Setyawati; Djarot Sasongko Hami Seno; Waras Nurcholis
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 6, No. 1, May 2020
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (763.755 KB) | DOI: 10.15408/jkv.v6i1.9440

Abstract

Glucose oxidase is used in various industries for the development of enzymatic fuel cell. Based on prior studies, this compound is sourced from the local isolates of Aspergillus niger IPBCC 08.610, although investigations on the encoding gene have not been conducted. The purpose of this research, therefore, is to identify and characterized the gene responsible for encoding glucose oxidase, in the aspect of sequence, length, and restriction patterns. This experiment involved the amplification of genomic DNA using specific primers for gene recognition, which was followed by the restriction technique with EcoRI and PstI endonucleases. Furthermore, the gene is inserted into vector pGEM®T-Easy and transformed into competent E. coli DH5α cells, in an attempt to perform sequencing. The glucose oxidase gene from A. niger IPBCC 08.610 was confirmed to possess a size of 1848 bp, and a GC content of 57.8%, with a possibility of restriction into two fragments of size 908 bp and 980 bp, using the EcoRI restriction.
Phytochemical Analysis, α-glucosidase Inhibition Activity in-vitro and Enzyme Kinetics of Ethyl Acetate and Hexane Extracts of Graptophylum pictum (L.) Griff Waras Nurcholis; I Made Artika; Djarot Sasongko Hami Seno; Dimas Andrianto; Apipah Aprianti; Fina Febrianti; Inawati Inawati; Antonius Padua Ratu; Arya Arendra
Current Biochemistry Vol. 1 No. 2 (2014)
Publisher : IPB University

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Abstract

The species Graptophylum pictum (L.) Griff, also known as “daun ungu” in Indonesia, is a traditional herbaceous plant believed to have antidiabetic potential. The number of people in the world with diabetes has increased dramatically over the recent years. The treatment of type II diabetes is complicated by several factors inherent to the disease. Elevated postprandial hyperglycemia is one of the risk factors and the intestinal digestive enzyme α-glucosidase plays a vital role in carbohydrate metabolism. One of the antidiabetic therapeutic approaches which reduces the postprandial glucose level in blood is by the inhibition of α-glucosidase. In this study, phytochemical analysis, α- glucosidase inhibitory activity and enzyme kinetics of ethyl acetate- and hexane extracts of G. pictum were evaluated with the aim to analyze its antidiabetic potential. Phytochemical analysis revealed the presence of tannins, steroids, and alkaloids. Steroids were present in ethyl acetate extract but absent in hexane extract, while alkaloids were present in hexane extract but absent in ethyl acetate extract. The ethyl acetate and hexane extracts had 30.68 and 49.82 % inhibitory effect on α-glucosidase activity respectively. The kinetics of glucosidase enzyme of ethyl acetate and hexane extracts were determined by Lineweaver Burk plots. These exhibited uncompetitive and noncompetitive inhibition to alpha-glucosidase activity respectively. From the enzyme assay, we infer that ethyl acetate and hexane extracts of G. pictum contain potential α-glucosidase inhibitors that have the potential to be exploited for use in the treatment of diabetes
Identification of Aroma Gene (Mutated badh2) and Properties of Aroma on Aromatic BC5F2 Ciherang Jap Mai Cing; Djarot Sasongko Hami Seno; Tri Joko Santoso
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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Abstract

Aromatic rice varieties have some weaknesses such as low productivity, and less resistant to pests and diseases. This study aimed to obtain homozygous strain of BC5F2 Ciherang aromatic through the identification of aroma gene (mutated badh2) and properties of the aroma. Ciherang paddy (nonaromatic paddy) was used as the female parent, whereas Mentik Wangi paddy (aromatic paddy) was used as the male parent. The experiment was conducted in BC5F2 because it is expected to generate plants with properties 98.4% close to female parent. The DNA from five strains of paddy plants BC5F2Ciherang X Mentik Wangi was isolated by a modified CTAB method. The concentration of DNA was determined by measuring absorbance at 260 nm wavelength, while its purity was determined from the ratio of the absorbance at a wavelength of 260/280 nm. PCR-based molecular selection was done by using the Bradbury primers. PCR results showed that of the 250 samples, there were 66 samples had DNA fragment of the same size as that of Mentik Wangi, i.e. 257 bp, 67 samples had the same size as the DNA fragment of Ciherang, i.e. 355 bp, and 117 samples had the same size with the both of DNA fragments, i.e. 257 bp and 355 bp. Plants with amplified 257 bp DNA fragment was subjected to leaf aroma test using 1.7% KOH. The results showed that 42 positive samples, out of 66 samples. Samples positive on leaf aroma test were tested again on rice aroma test. Rice aroma test results showed the majority (85.4%) samples that are positive on leaf aroma test is also positive on the rice aroma test. 
In Silico Analysis of Curcuma longa Against PCAF Histon Asetiltransferase Artha Vinsentricia; Djarot Sasongko Hami Seno; Maria Bintang
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

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Abstract

Curcumin turmeric (C. longa) has been found capable of inhibiting p300/CBP associated factor (PCAF) histone asetyltransferase (HAT) as a cancer biomarker. However, the molecular mechanism of inhibition is unkown. This study is aimed to analyse the molecular inhibition mechanisms by in silico method. The analysis showed that curcumin’s Gibbs free energy (ΔG) was stable and capable of binding amino acid residues believed to be active site of PCAF HAT. Curcumin is predicted to be a PCAF HAT inhibitor, and further in vitro analysis is required.
The Activity of Wungu Leaf (Graptophyllum pictum (L) Griff) Extract in Reducing Blood Glucose Level of Hyperglycemic Mice Hayatul Rahmi; I Made Artika; Norman Razief Azwar; Djarot Sasongko Hami Seno; Waras Nurcholis
Current Biochemistry Vol. 1 No. 2 (2014)
Publisher : IPB University

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Abstract

Wungu leaf (Graptophyllum pictum (L.) Griff) is a plant thought to have potential use in alleviating symptoms of diabetes mellitus. The purpose of the present study was to evaluate the activity of wungu leaf extracts in decreasing blood glucose level of alloxan (200 mg/kg BW)-induced hyperglycemic mice. Extracts of wungu leaf were obtained by macerating with ethanol and then partitioning the extract with diethyl ether, ethyl acetate, and butanol. Each extract obtained was used to treat hyperglycemic mice for 28 days. The results showed that wungu leaf extracts have the ability to decrease the blood glucose level of hyperglycemic mice (dose 50 mg/kg BW). The ethyl acetate extract showed the highest activity, bringing about a decrease of blood glucose of 37.6 %. The wungu leaf extract has the potential to be developed as a source of anti-diabetic agents.
Co-Authors . Sugihartati . Taufiq A.A. Ketut Agung Cahyawan W Akhmad Endang Zainal Hasan Aniversari Apriana Aniversari Apriana Annisa Dhiya Athiyyah Khanza Antonius Padua Ratu Apipah Aprianti Artha Vinsentricia Arya Arendra Bambang Padmadi Bambang Pontjo Priosoeryanto Bram Kusbiantoro Cika Hilda Fransiski Dewi Praptiwi Dimas Adrianto Dimas Adrianto DIMAS ANDRIANTO Dwitasari, Oczhinvia Dwitasari, Oczhinvia Euis Marlina Fina Febrianti Hayatul Rahmi Helmy Ramadhan Al Anshary I MADE ARTIKA Inawati Inawati Inda Setyawati, Inda Jap Mai Cing Joel Rivandi Sinaga John Douglas Brooker Kurniatin, PA. LAKSMI AMBARSARI MARIA BINTANG Maria Bintang MEGA SAFITHRI Mega Safithri Mega Safithri Muhammad Taufan Fatahajudin Mustika Weni Najmah Najmah Norman Razief Azwar Oczhinvia Dwitasari Rini Kurniasih Riyan Alifbi Putera Irsal Rizki Fitria Rudi Munzirwan Satya Nugroho Satya Nugroho Tri Joko Santoso Tri Joko Santoso Waras Nurcholis Zainal Alim Mas'ud Zainal Alim Mas'ud