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PREVALENCE OF DIGESTIVE ENDOPARASITES (HELMINTH AND PROTOZOA) IN PIGEON DOMESTIC (Columba livia) MALE AND FEMALE IN TUBAN Melanie Aulia Ashfiyah; Setiawan Koesdarto; Hana Eliyani; Mufasirin Mufasirin; Emy Koestanti Sabdoningrum
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v6i1.34102

Abstract

This research was conducted to determine the prevalence of major gastrointestinal helminthiasis and protozoal infections, the kinds of helminth and protozoa that infects and the effect of sex on pigeons in Tuban. Pigeons are taken from five The Tuban area is Widang, Montong, Jenu, Soko, Jatirogo. Each area is taken as many as 20 pigeons consists of 10 male pigeons and 10 female pigeons. Laboratory examinations were carried out in November to December 2021. Samples were examined by the native method, sedimentation, and flotation. Identification using a microscope using a 40x objective scale and 10x ocular scale. Data analysis using chi square test statistical analysis. 81% of the samples were positive for helminthiasis infection. Chi square test statistical analysis showed p value 0.022 (p<0.05) which means significantly different. 71% of samples were positive for gastrointestinal protozoa infection in pigeons in Tuban. Chi square test Results Statistical analysis shows p the value of 0.123 (p>0.05) which means that it is not significantly different. Type of protozoa found that Heterakis gallinarum (45%), Ascaridia columbae (42%), Capillaria sp. (41%), and Eimeria sp. (71%). That prevalence of helminthiasis in male pigeons higher (45%) compared to female pigeons (36%).
Sambiloto (Andrographis paniculata) Extract Improves the Performance of Animal Model Infected with Escherichia coli Sri Hidanah1 , Emy Koestanti Sabdoningrum1 , Mohammad Anam Al Arif1 , Arif Nur Muhammad Ansori2 , Ta
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12167

Abstract

This study aims to understand the effect of natural antibiotics from sambiloto (Andrographis paniculata)extract to laying hens infected with Avian Pathogenic Escherichia coli (APEC). The activation test ofsambiloto was performed by using a dilution method included a minimum inhibitory concentration (MIC)and a minimum bactericidal concentration (MBC). Laying hens were distributed into five groups: normalgroup, APEC group with no treatment, and APEC group given 10%, 20%, 30% sambiloto extract for twoweeks. The data were analyzed using ANOVA and continued with the F test. Based on the results, the 30%extract has improved the hen day production (HDP) of laying hens infected with APEC.
INSEMINASI BUATAN MENGGUNAKAN PERSILANGAN SPERMA LIMOSIN DAN SIMENTAL MELALUI SINKRONISASI BIRAHI SUATU USAHA INTENSIFIKASI REPRODUKSI SAPI UNTUK PENINGKATAN PETERNAKAN SAPI RAKYAT DI KECAMATAN KEDUNGADEM KABUPATEN BOJONEGORO Emy Koestanti Sabdoningrum; Sunaryo Hadi Warsito; Heru Pramono; Siti Eliana
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 1 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.27 KB) | DOI: 10.20473/jlm.v2i1.2018.6-11

Abstract

The beef cattle breeding business in Kedungrejo village, Kedungadem sub-district, Bojonegoro Regency in general has not implemented an efficient business concept considering that there are many potentials and opportunities that have not been utilized and managed optimally, especially those related to livestock reproduction and beef cattle production. Constraints that are often encountered are the low success rate of Artificial Insemination (IB) of beef cattle because poor quality cement and inseminator skills are not sufficient. Such conditions are also an illustration of the Beef Cattle Breeders in Kedungrejo Village, Kedungadem District, Bojonegoro Regency. Kedungrejo village was chosen to be a place for cattle development on dry land because it has the potential to breed cattle crosses of Lemosin and Simental cattle which produce good meat. The development of cattle in Kedungrejo village, Kedungadem sub-district, aims to increase economic activities in support of cattle reproduction intensification efforts to increase people’s cattle farming. This activity for the community aims to fulfill meat self-sufficiency through the introduction, dissemination, transfer of reproductive technology by artificial insemination using Limousine and Simental sperm crosses. Beef cattle farmers in Kedungrejo Village Kedungadem Bojonegoro Subdistrict have the prospect of beef cattle breeding which can be developed to fulfill meat self-sufficiency that is being promoted. Specific targets of outcomes to be achieved include the success of insemination, increased birth rate, improvement of genetic quality of beef cattle through Artificial Insemination using Limousine and Simental sperm crosses in the Kedungrejo Village, Kedungadem Bojonegoro District. The method used in achieving this goal by socializing Artificial Insemination using Limousine and Simental Sperm Crosses. The solution offered is through the coaching, training and application stages. The results of community service activities indicate that Artificial Insemination using Limousine and Simental Sperm Crosses has the prospect of being developed as an increase in the success of insemination, increased birth rate, genetic quality improvement has a positive influence on beef reproductive and meat self-sufficiency.AbstrakUsaha peternakan sapi potong di desa Kedungrejo Kecamatan Kedungadem Kabupaten Bojonegoro pada umumnya belum menerapkan konsep usaha yang efi sien mengingat banyak potensi dan peluang yang belum dimanfaatkan dan dikelola secara optimal terutama yang terkait reproduksi ternak dan produksi ternak sapi potong. Kendala yang sering dijumpai adalah rendahnya tingkat keberhasilan Inseminasi Buatan (IB) ternak sapi potong karena kualitas semen yang kurang baik dan skill inseminator belum memadai. Kondisi demikian juga merupakan gambaran dari Peternak Sapi Potong Desa Kedungrejo Kecamatan Kedungadem Kabupaten Bojonegoro. Desa Kedungrejo dipilih menjadi tempat pengembangan ternak sapi di lahan kering karena mempunyai potensi untuk pemuliabiakan persilangan sapi Lemosin dan sapi Simental yang menghasilkan daging yang bagus. Pengembangan ternak sapi di desa Kedungrejo Kecamatan Kedungadem bertujuan meningkatkan kegiatan perekonomian dalam mendukung usaha intensifi kasi reproduksi sapi untuk peningkatanpeternakan sapi rakyat. Kegiatan kepada masyarakat ini bertujuan pemenuhan swasembada daging melalui pengenalan, penyebarluasan, alih teknologi reproduksi dengan cara Inseminasi buatan menggunakan persilangan sperma Limousin dan Simental. Peternak sapi potong Desa Kedungrejo Kecamatan Kedungadem Bojonegoro mempunyai prospek peternakan sapi potong yang dapat dikembangkan untuk memenuhi swasembada dagingyang sedang digalakkan. Target khusus luaran yang ingin dicapai berupa keberhasilan inseminasi, peningkatan angka kelahiran, perbaikan mutu genetik sapi potong melalui Inseminasi Buatan menggunakan persilangan sperma Limousin dan Simental di wilayah Desa Kedungrejo Kecamatan Kedungadem Bojonegoro. Metode yang dipakai dalam pencapaian tujuan tersebut dengan sosialisasi Inseminasi Buatan menggunakan Persilangan Sperma Limousin dan Simental. Solusi yang ditawarkan adalah melalui tahap pembinaan, pelatihandan aplikasi. Hasil kegiatan pengabdian kepada masyarakat ini menunjukkan bahwa Inseminasi Buatan menggunakan Persilangan Sperma Limousin dan Simental mempunyai prospek untuk dikembangkan sebagai peingkatan keberhasilan inseminasi, peningkatan angka kelahiran, perbaikan mutu genetik memberi pengaruh positif terhadap reproduktivitas sapi potong dan pemenuhan swasembada daging.
Novel Combination of Andrographis paniculata and Phyllanthus niruri to Improve Performance of Laying Hens Infected with Escherichia coli Sri Hidanah1 , Emy Koestanti Sabdoningrum1,2, Sri Chusniati3 , M. Bagus Kurniawan Saputra4
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13637

Abstract

The study aims to learn the efficacy combination of Phyllanthus niruri and Androgaphis paniculata toimprove the performance of laying hens infected with Escherichia coli pathogen. Fifty laying hens of IsaBrown strain were randomly divided into 5 treatments, each treatment was divided into 10 replications(n=10). Treatment P0- (control group without infected), P0+ (hens group infected with Avian Pathogenic’sEscherichia coli without given extract), P1 (hens group infected with Avian Pathogenic’s Escherichia coliwith 10% Phyllanthus niruri extract and 30% Androgaphis paniculata), P2 (hens group infected with AvianPathogenic’s Escherichia coli with 20% Phyllanthus niruri extract and 20% Androgaphis paniculata) andP3 (hens group infected with Avian Pathogenic’s Escherichia coli with 30% Phyllanthus niruri extract and10% Androgaphis paniculata). Performances observed were feed consumption, Hen Day Production, eggsweight and feed conversion. Data analyzed by ANOVA and tested with the F test. The feed consumptionshowed in P1 was different from P3, P2, P0+ and P0-, P1 was different from P2 but not with P3, P0+, andP0- showed significantly different in each treatment. In P3 showed no differences with all treatments. Theeggs weight showed different in P0+ for all treatments, while the other treatments in P1, P2, P3 and P0-showed no differences. The feed conversion showed in P0+ was different for all treatments, while othertreatments showed no difference. P0+ treatment compared to (P0-, P1, P2) was significantly different, P0+treatment compared to P3 treatment was not significantly different, between P3 and P1 treatment was notsignificantly different, and between P1 and P2 with P0- was not significantly different, also between P3 withP2 and P0- treatments was significantly different. Supply of P. niruri extract. and A. paniculata in layinghens can improve the performance of laying hens infected with Escherichia coli pathogen.
In Silico Study : Phyllanthus Niruri L as Immunomodulator Against Covid-19 Mohammad Sukmanadi1 , Emy Koestanti Sabdoningrum2 aditya Tri Ananda3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12095

Abstract

In December 2019, a mysterious case of pneumonia was first reported in Wuhan, Hubei Province. Thesource of transmission of this case is still unknown, but the first case was linked to the fish market in Wuhan.From 18 December to 29 December 2019, there were five patients treated with Acute Respiratory DistressSyndrome (ARDS). 2 From 31 December 2019 to 3 January 2020 this case increased rapidly, marked by 44cases reported. In less than a month, the disease has spread to other provinces in China, Thailand, Japan andSouth Korea. This virus can be transmitted from human to human and has spread widely in China and morethan 190 other countries and territories. On 12 March 2020, WHO announced COVID-19 as a pandemic.As of March 29, 2020, there were 634,835 cases and 33,106 deaths worldwide. While in Indonesia, 1,528cases were confirmed with COVID-19 and 136 deaths. Phyllanthus niruri L (meniran) is one type ofimmunostimulator that can improve the immune system in animal experiments and humans. This studyaims to determine Phyllanthus niruri L as an immunomodulator for Covid-19. From the results of researchconducted that Phyllanthus niruri L can improve the activities and functions of several non-specific immunesystem components and specific immune systems, both soluble and cell-related forms. The conclusion fromthe results of this study is Phyllanthus niruri L as an immunomodulator for Covid-19.
PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM KUNING TELUR TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Wahyu Retno Swari; Emy Koestanti Sabdoningrum; Wurlina Wurlina; Suherni Susilowati; Rochmah Kurnijasanti; Erma Safitri
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.407 KB) | DOI: 10.20473/ovz.v8i2.2019.122-126

Abstract

The purpose of this study was to determinated the effect and the best concentration of greentea extract in skim milk and egg yolk diluent for quality sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups; skim milk and egg yolk diluent, 0,5% green tea extract in skim milk and egg yolk diluent, 0,1% green tea extract in skim milk and egg yolk diluent, and 0,15% green tea extract in skim milk and egg yolk diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after being diluted. The data obtained was analyzed with the Analysis of Variant (ANOVA), followed by multiple range test duncan. The results showed that the highest percentage of motility, viability and intact plasma membrane derived from green tea extract 0,15% on skim milk and egg yolk diluent  for 1 day storage is 86.66b ± 2.58, 92.00b ± 1.78, and 74.16b ± 3.25. The lowest percentage of motility, viability and intact plasma membranes was obtained from the skim milk and egg yolk diluent for 5 days storage is 43.33a ± 6.05, 56.50a ± 3.08 and 28.33a ± 2.80. The conclusion of this study is the addition of 0.15% green tea extract in a skim milk and egg yolk diluent can maintain the quality of sperm for up to 5 days of storage at cold temperatures.
PEMBERIAN EKSTRAK MENIRAN (Phyllanthus niruri Linn) TERHADAP GAMBARAN HISTOPATOLOGI INFUNDIBULUM AYAM PETELUR YANG DIINFEKSI Escherichia coli Adinda Riezki Irawan; Emy Koestanti Sabdoningrum; Sri Hidanah; Sri Chusniati; Sri Pantja Madyawati; Benjamin Christoffel Tehupuring
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19992

Abstract

The purpose of this research aimed to prove the effect of meniran extract (Phyllanthus niruri Linn) on layer hen infected with Escherichia coli to reduce inflammation and congestion on infundibulum histopathological view.  This research used 20 samples of layer hen. The treatment in this research was P0- (not infected with Esherichia coli and did not treated with meniran(Phyllanthus niruri Linn)), P0+ (infected with Escherichia coli and did not treated with meniran(Phyllanthus niruri Linn)), P1 (infected with Escherichia coli and treated with 10% meniran extract (Phyllanthus niruri Linn)), P2 (infected with Escherichia coli and treated with 20% meniran extract (Phyllanthus niruri Linn)), P3 (infected with Escherichia coli and treated with 30% meniran extract (Phyllanthus niruri Linn)). The data of this study analyzed by Kruskal-wallis and followed with Mann-withney test. The result of this research can be concluded that 10% concentration of meniran extract (Phyllanthus niruri Linn) was effective to eliminate Escherichia coli, 20% concentration of meniran extract (Phyllanthus niruri Linn) able to promote antibacterial activity, and 30% concentration of meniran extract shown antibacterial rate on high potential so it can be used to reduce inflammation and congestion as the result of Escherichia coli infection.
New Biotype of Vibrio cholerae O1 from Clinical Isolates in Surabaya Garry Cores de Vries; Emy Koestanti Sabdoningrum; Dadik Rahardjo
Indonesian Journal of Tropical and Infectious Disease Vol. 1 No. 1 (2010)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (646.647 KB) | DOI: 10.20473/ijtid.v1i1.3724

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A surveillance of new pathogenic variants of Vibrio cholerae O1 strains was initiated to identify the emerge and spread throughout Surabaya. Findings from seven years (1994–2000) and from years 2008 until now by using a two-fold surveillance strategy was pursued involving 1) hospital-based case recognition, and 2) environment samples. Rectal swabs and environment samples were transported to ITD-UNAIR, Surabaya for culture and isolates were characterized by serotypic identification and arbitrarily primed PCR fingerprints revealed a group of strains with similar fingerprint patterns that are distinct from those of the current El Tor epidemic strain. These strains have been analyzed by in vitro technique and the group has been denominated the Surabaya-Indonesian variant of V. cholerae O1.
Peningkatan Respons Imun Non Spesifik Ikan Gurame Pascapemberian Ekstrak Air Panas Mikroalga Spirulina platensis (ENHANCEMENT OF NON-SPECIFIC IMMUNE RESPONSE OF OSPHRONEMUS GOURAMY AFTER GIVING OF HOT WATER EXTRACT SPIRULINA PLATENSIS) Woro Hastuti Satyantini; Agustono Agustono; Arimbi Arimbi; Emy Koestanti Sabdoningrum; Myrna Budi; Lina Wafia Asmi
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.344 KB)

Abstract

Hot water extract of Spirulina platensis which is contain of lipopolysaccharide can be used as animmunostimulant. This study aimed to determine the effect of hot water extract of S. platensis throughinjection on the non-specific immune response of Osphronemusgouramy. The treatment was given throughintraperitoneal injection of hot water extract of S. platensis with doses of 1%, 10%, 20% and PBS, PhosphateBuffer Saline (control) 0.1 ml/fish. Fish reared in the aquarium (67 l volune) with density 12 fishes/aquarium with average weight of 7.5 – 8.5 g/fish. Blood samples of fish taken from the caudal vein on day0 (before administration of the extract), 3 and 5 days after administration of the extract for the measurementof total leucocytes, differential leucocytes and phagocytic activity. Results showed an increased in totalleucocytes, phagocytic activity, monocytes and neutrophils of fish that given hot water extract of S. platensis.The addition of hot water extract of S. platensis at 1% through injection gave total leucocytes, phagocyticactivity, monocytes and neutrophils on days 3 were 543.73 x 103sel/mm3,69.30%, 34.33% and 14%respectively higher and significantly different to control.
EFFECTIVENESS OF MENIRAN (PHYLLANTHUS NIRURI LINN) AS ANTIBACTERIAL FOR RESISTANCE ANTIBIOTICS PREVENTION OF ENTEROTOXIN ESCHERICHIA COLI Sri Hidanah; Emy Koestanti Sabdoningrum; Retno Sri Wahyuni; Arini Rahmi Dewi; Erma - Safitri
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 2 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.153 KB) | DOI: 10.20473/ijtid.v7i2.7328

Abstract

Escherichia coli (E. coli) can be isolated from the environment both inside and outside the hospes body. There were 89 serotypes in which showed 21% resistance to various antibiotics, such as enterotoxigenic E.coli. Alternative efforts are needed to overcome this, one of them through the use of medicinal plants, such as meniran (Phyllanthus niruri Linn). Meniran plant is an immunomodulator that serves to repair the immune system of the body. The aim of research is the research was done through several stages: isolation and identification of enterotoxigenic E. coli from several broiler farms in East Java using the polymerase chain reaction (PCR) method, E. coli resistance test against some antibiotics, making meniran extract and activation test against enterotoxigenic E. coli n The study was divided into five treatments: T0+ (group of chickens were infected by enterotoxigenic E. coli T0- (control group, not infected), T1 (infected by enteroxigenic E. coli + 20% meniran extract), T2 (infected by enterotoxigenic E. coli + 25% extract meniran), T3 (infected by enterotoxigenic E. coli + 30% extract meniran). Data were analyzed by ANOVA (Analysis of Variance). The results show that all of E. coli DNA isolates which tested by the PCR method show positive reactions at 600 bp. The next stage, the enterotoxigenic E. coli are resistant to some antibiotics, such as amoxicillin, ampicillin, erythromycin, cephalosporins, tetracycline, cloxacillin and gentamicin. Furthermore, the 30% Phyllanthus niruri Linn extract is effective as an antibacterial for antibiotic resistance enterotoxigenic E. coli. next necessary to write that: The 30% meniran extract is potential for kill of enterotoxigenic E. coli