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All Journal Dentika Dental Journal Indonesian Journal of Biotechnology Journal Of Syiah Kuala Dentistry Society Cakradonya Dental Journal

Endang Winiati Bachtiar

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Author-ID : 259742

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Public Health

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EFFECT OF CORAL GONIOPORA IN COMPARISON WITH CORAL APATITE TOWARDS HUMAN DENTAL PULP STEM CELLS MINERALIZATION ACTIVITIES: EFEK CORAL GONIOPORA DIBANDINGKAN DENGAN CORAL APATITE TERHADAP AKTIVITAS MINERALISASI SEL STEM PULPA GIGI Rachmi Fanani Hakim; Endang Winiati Bachtiar; Nurtami Soedarsono
Dentika: Dental Journal Vol. 17 No. 1 (2012): Dentika Dental Journal
Publisher : TALENTA

Challenging approach in tissue engineering for dentin regeneration is focused upon the application of a scaffold on anopen pulp enabling odontoblast-like cells to grow into the scaffold and to convert them into dentin-like substance. Coralwas chosen as a scaffold because of its good biocompatibility and resorbability. The species of marine invertebratesexploited in medical applications are Members of Porites and Goniopora. Coral goniopora is most marine invertebratafound in Indonesia's marine. Coral apattite, an osteoconductive synthetic bone graft substitute material, is manufacturedby the hydrothermal conversion of the calcium carbonate skeleton of coral to hydroxyapattite in the presence ofammonium phosphate preserving the original porous structure which is similar to that of bone. The aim of study was toinvestigate the effect of Coral goniopora and coral apattite as a potential scaffold on dental pulp mineralization activity. Invitro DPSCs mineralization activity was measured by von Kossa staining for calcium deposit identification. The resultthat Coral apattite increased more calcium deposited identification than coral goniopora. Calcium deposited on dentalpulp stem cells are marker for mineralized dental pulp stem cells (DPSCs). Mineralized DPSCs are marker forodontoblast diferentiation and maturation. In conclusion, these observations demonstrated that co-cultured coral apattiteand DPSCs induced a better mineralization activity than those cultured with Coral goniopora.

Construction and Immunogenicity Testing of Salmonella, STM1 Vaccine Vector Expressing HIV-1 Antigen Endang Winiati Bachtiar; Peter Smooker; Peter J Coloe
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

Objective of this study: to determine the ability of Salmonella enterica serovar Typhimurium STM1 as a delivery vehicle for the HIV p24 gene and HIV env gene. The STM1 delivery HIV-p24 vaccination was carried out in the form of a recombinant or a DNA vaccine whereas only a DNA vaccine was used for HIV env. Naked DNA vaccination was also tested and immune responses were evaluated following immunisation in mouse model. Results: vaccination cellular immune responses induced by recombinant p24 STM1 (STM1/pHly-p24) were greater than those elicited by the p24 DNA vaccine in STM1 (STM1/VR-p24), (but statistically not significance) than those induced by oral vaccination. However, IgA responses induced by oral vaccination with either a recombinant or DNA vaccine of p24 in STM1 are higher than those induced by IP vaccination. Conclusions: This result confirms</div><div>other studies that Salmonella was able to deliver HIV antigens to the immune system and induced specific immune responses to the HIV antigen.

Antigen Presentation Ability of Salmonella Carrying DNA Vaccine Model and MCP-3 gene Endang Winiati Bachtiar; Peter Smooker; Peter J Coloe
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

The objective of this study is to determine the antigen presentation ability of a DNA vaccine model that is co-delivered with that of recombinant Salmonella enterica serovar Typhimurium (STM1) expressing chemokine macrophage chemotactic protein-3 (MCP-3). The DNA vaccine, pVROVA, was constructed by amplification of the ovalbumin coding region from sOVA-C1. Dendritic cells (DCs) were obtained from IL-4 and GMCSF stimulated mouse bone marrow stem cell. Cultured DCs were incubated with STM1 carrying a model ovalbumin gene (pVROVA). Furthermore, MHC class I antigen presentation of a dominant OVA peptide was assayed in vitro. The experiments were designed to determine the effect of co-delivering MCP-3 with that of ovalbumin in STM1. Our results show that a plasmid pROVA-carrying ovalbumin gene was succesfully constructed and sequence analysis of the ovalbumin-coding revealed an identity match of 100% with that of the chicken ovalbumin DNA sequences from the GenBank database. We also found that the presence of the MCP-3 encoding plasmid in STM1 or E. coli DH1 could increase the recovery of both STM1 and E. coli DH1 over those that carry the empty plasmids. Antigen presentation assay also indicates that MCP-3 can positively influence the presentation of ovalbumin. Conclusion: the infection of DCs by STM1-carrying DNA vaccine and MCP-3 results in an increase of processing and presentation of ovalbumin in vitro.Keywords : DNA vaccine, MCP-3, APC, Salmonella, Dendritic cells

POTENSI CIGARETTE SMOKE CONDENSATE TERHADAP PENINGKATAN PEMBENTUKAN BIOFILM CANDIDA albicans ISOLAT ATCC 10261 Basri A.Gani; Annisa Qashdina Alghassani; Zaki Mubarak; Endang Winiati Bachtiar; Boy M.Bachtiar
Journal of Syiah Kuala Dentistry Society Vol 2, No 1 (2017): JANUARY
Publisher : Dentistry Faculty

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Candida albicans (C. albicans) is the main agent in pathogenesis of oral candidiasis, and also the cigarette smoke is one of predisposing factors of C. Albicans virulence, mainly in biofilm formation that it the role as the initiator of adhesiononoral mucosal. This research aimed to identify the effect of Cigarette smoke condensate (CSC) on biofilm formation of C. albicans. Candida albicans was sensitized with CSC kretek and non-kretek that shown the increase of biofilm formation (p0,05) compared control, specifically in 24 hour. It’s related with massa of biofilm formation that seem by microscope (400x) and also CSC non kretek better than CSC kretek in biofilm formation of C. albicans, mainly in 24 and 48 hours, that provisional, in 12 hours were the most dominant biofilm figuration of C. albicans sensitized by CSC kretek, although it intensity is lower than 24 hours. The research was concluded that CSC can be trigger to enhancement of biofilm formation of C. albicans in 24 hours and also CSC non kretek better than CSC kretek.Keyword: Candida albicans, Cigarrete smoke condensate, Biofilm

PORPHYROMONAS GINGIVALIS DAN PATOGENESIS DISFUNGSI KOGNITIF: ANALISIS PERAN SITOKIN NEUROINFLAMASI Citra Feriana Putri; Endang Winiati Bachtiar
Cakradonya Dental Journal Vol 12, No 1 (2020): Februari 2020
Publisher : FKG Unsyiah

Porphyromonas gingivalis merupakan bakteri utama penyebab penyakit periodontal. Bakteri ini memiliki faktor virulensi seperti lipopolisakarida dan gingipain. Faktor virulensi ini dapat membuatnya lolos dari sistem imun periodontal lalu masuk ke dalam sirkulasi darah atau bakterimia. Bakterimia yang terjadi kemudian menyebabkan bakteri berpindah ke sistem saraf pusat sehingga mengaktivasi mikroglia dan respon neuroinflamasi. Neuroinflamasi merupakan proses inflamasi kompleks yang terjadi di sistem saraf pusat sebagai mekanisme pertahanan dalam melawan patogen, toksin, atau faktor yang menyebabkan neurodegenerasi. Proses neuroinflamasi ini diregulasi oleh aktivitas sel neuron, glia, dan sel endotel di dalam sistem neurovaskular. Produksi mediator inflamasi yang berlebihan oleh sel-sel tersebut dapat menyebabkan kematian sel neuron dan berhubungan dengan terjadinya disfungsi kognitif, seperti penyakit Alzheimer. Dalam tinjauan pustaka ini akan dipaparkan tentang imunopatogenesis Porphyromonas gingivalis pada disfungsi kognitif melalui aktivasi neuroinflamasi di otak.

Co-Authors Annisa Qashdina Alghassani Basri A.Gani Boy M.Bachtiar Citra Feriana PUTRI Nurtami Soedarsono Peter J Coloe Peter Smooker Rachmi Fanani Hakim Zaki Mubarak

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