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All Journal Jurnal Kedokteran Syiah Kuala Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana INDONESIAN JOURNAL OF PHARMACY Jurnal Surya Medika Warta Pengabdian JURNAL ILMU KEFARMASIAN INDONESIA Harmony : Jurnal Pembelajaran IPS dan PKN Jurnal Pengabdian Masyarakat Asia Berkala Ilmiah Kimia Farmasi Berkala Penelitian Hayati Clinical, Analytical, Pharmaceutical, and Pharmacy Community Journal

Sudjarwo Sudjarwo

Faculty Of Pharmacy, Airlangga University

Author-ID : 2598637

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Analisis Antimullerian Hormon (AMH) di Dalam Serum pada Berbagai Kategori Indeks Masa Tubuh Didik Rio Pambudi; Ashon Sa’adi; Sudjarwo Sudjarwo
Jurnal Surya Medika (JSM) Vol 5 No 1 (2019): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Obesity-related to the result of decreased reproduction. Obese women are more prone to abnormal anovulation and uterine bleeding, endometrial hyperplasia/cancer, infertility, miscarriage, and pregnancy complications, compared to women of normal weight. This study aims to determine the levels of anti-Mullerian hormone (AMH) in the serum at various BMI (body mass index), also to determine the relationship and correlation between obesity and AMH levels in serum. The population in the study were women aged 20 years to 40 years with less BMI, Normal BMI and Obesity BMI. The study subjects were women between the ages of 20 to 40 years with a BMI less than 17-19.9, obesity BMI ≥ 25 and normal BMI 20-25 as controls. Height measurement, weight weighing, and BMI calculation carried out according to the standard and subject to approval. Taking blood samples for the examination of AMH levels carried out by the RSKI laboratory (Infection Special Hospital) Airlangga University. The results of the data processed with SPSS 25 with the Shapiro-Wilk normality test and Mann Whitney statistical analysis for different tests and Spearman analysis for the correlation test. The results of the study found homogeneous samples, there were no significant differences between the AMH levels of the less and obese groups with, p = 0.832 (p> 0.05). AMH levels in BMI were less (0.459 ± 0.112 ng / mL) than obesity BMI (0.432 ± 0.058 ng / mL), so it was concluded that AMH levels did not correlate with less BMI with obesity BMI, with a correlation value (r) = -0.105 (p = 0.643; p> 0.05). The results of this study concluded that body mass index not related and does not correlate with the levels of anti-Mullerian hormone in the serum.

Verifikasi Metode ELISA (Enzym Linked Immunosorbent Assay) Untuk Penentuan Kadar AMH (Anti Mullerian Hormone) Irvan Ipandi; Ashon Sa'adi; Sudjarwo Sudjarwo
Jurnal Surya Medika (JSM) Vol 5 No 1 (2019): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Validation is the process of confirming that the method used has capabilities that are consistent with what is needed while being used continuously. The AMH ELISA kit has carried out a method validation process before marketed and used in a laboratory to determine AMH levels. AMH is a specific biomarker used to diagnose PCOS patients. The laboratory can adopt a validated procedure but the laboratory still needs to confirm its ability to apply this method. Verification is the provision of objective evidence that the measured parameters meet the specified requirements. The purpose of this study was to find out how well verification of the ELISA AMH kit and comparing the calculation of 4 PL with linear regression. Based on the statistical test obtained p = 0.871 (p>0.05). It can be said that there was no significant difference between Optical Density days 1 and 7(p = 0.05). Linear regression equation (r) 0.9543 (p = 0.000; p <0.05) while in equation 4 PL (r) 1,000. The ELISA kit AMH method meets the verification requirements. The 4PL model calibration curve is more suitable to be used in the AMH ELISA kit than the linear regression model.

PeranSenyawa Oksigen Reaktif dalam Makanisme KerusakanIntegritas Membran Spermatozoa KerbauLumpur Setelah Sentrifugasi Gradien Densitas Percoll Dasrul Dasrul; Hardjopranjot Hardjopranjot; Mahaputra L; Sudjarwo4 Sudjarwo4
Jurnal Kedokteran Syiah Kuala Vol 8, No 2 (2008): Volume 8 Nomor 2 Agustus 2008
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstrak. Penelitian ini bertujuan untuk menganalisa senyawa oksigen reaktif dan pengaruhnya terhadap integritas membran plasma spermatozoa kerbau lumpur basil sentrifugasi gradien densitas percoll. Penelitian ini menggunakan semen segar kerbau lumpur sehat yang diperoleh dengan cara penampungan menggunakan vagina buatan. Sampel semen dibagi dalam tiga kelompok yaitu tanpa sentrifugasi (kontrol) sentrifugasi gradien densitas percoll selama 5 menit dan sentrifugasi gradien densitas percoll selama 10 menit. Selanjutnya semenbasil sentrifugasi diamati produksi senyawa oksigen reaktif dan integritas membran spermatozoa.Hasil penelitian menunjukan bahwa produksi ROS spermatozoa setelah sentrifugasi gradien densitas percoll meningkat secara bermakna {p0,05), sedangkan persentase membran plasma utuh menurun secara bermakna{p0,05). Tingkat produksi ROS dan penurunan persentase integritas membran plasma utuh spermatozoa kerbau lumpur setelah sentrifugasi gradien densitas percoll selama IO menit lebih tinggi secara bermakna (p0,05) dibandingkan dengan sentrifugasi gradien .d1;nsitas percoll selama 5 menit.. Tingkat produksi ROS berkorelasi negatif'dengan persentase integritas membran palsma utuh spermatozoa. (JKS 2007; 2:69-80) Kata Kunci: Sperma kerbau lumpur; sentrifugasi gradien densitas percoll, ROS, integritas membran plasma utuh Abstract The objective of this research was to find out the effect of preparation of sperm by using percoll gradient density centrifugation on the of reactive oxygen species (ROS) production and the demage integrity membranre of sperm swamp buffalo.The research is a laboratory study using a true experimental design, used swamp buffalo of sperm divided into 3 groups i. E ( no centrifugation/control; percoll density gradient centrifugation for 5 minutes and percoll density gradient centrifugation for IO minutes. Subsequently a determination of ROS production and· demage membrane integrity of sperm. The colleted data in this reseacrh were analysed varians and regression analysis. The result showed that ROS production after preparation with percoll density gradient centrifugation was highest of significant {p0,05) and the procentage intact membrane of sperm a significant decreased (p0,05). The increase of ROS concentration and decrease. of procentage plasma membrane intact were after percoll density gradient centrifugation for IO minutes increase significantly (p0,05) compared with percoll density gradient centrifugation for 5 minutes. The ROS concentration of spermatozoa has correlated with the prosentage of membrane plasma intact of spermatozoa after percoll density gradient centrifugation. (JKS 2007; 2:69-80)Keywords: Sperm of swamp buffalo; perco/1 gradien density centrifugation, ROS, integrity membrne plasma intact

THE OPTIMATION OF SPECTROFLOUROMETRIC METHOD FOR DETERMINATION OF MALONIC DIALDEHYDE IN HUMAN SPERM Sudjarwo .
Indonesian Journal of Pharmacy Vol 12 No 3, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Optimation of a method is an obligatory step in order one to obtain a valid and accountable data, especially when one deal with biological samples. Biological samples usually contain minute substances which sometime elicit great errors when someone try to determine its concentration. Therefore, in order to reduce those errors, optimation of a method is mandatory to be carried out. The aim of this experiment was to determine malonic dialdehyde (MDA), a product of lipid peroxidation. The optimation carried out in this experiment include the selection of wavelength of maximum absorbance, the amount of sodium thiobarbiturate, pH, the incubation period, standard curve linearity, limit of detection and quantification, accuracy and precision. The result showed that the optimum conditions of spectrofluorometric method of MDA were as follow: the excitation and emission wavelengths was 522 and 575 nm, respectively, pH of 1 and incubation period of 135 minutes. These conditions yielded correlation coefficient of standard curve of 0.9993, limit detection and quantification of 3.5146 x 10-4 mg/ml and 1.0544 x 10-3 mg/ml, respectively, an average recovery of 85.01 % and variation coefficient of 1.84 %.Key words: optimation, spectrofluorometric, sperm, malonic dialdehyde, MDA

Toxicity of 32.2 kDa MW Escherichia coli Pili Adhesin Isolated from Infertile Male Semen in Reproductive System Sukarjati Sukarjati; Susie Amilah; Sudjarwo Sudjarwo
Folia Medica Indonesiana Vol. 54 No. 2 (2018): June
Publisher : Faculty of Medicine, Universitas Airlangga

Escherichia coli (E. coli) is the leading cause of male genital tract infection with no symptoms of infertility. Protein E. coli pili hemagglutinin isolated from infertile male sperm with 32.2 kDa MW acts as adhesion in spermatozoa. This study aimed to prove whether E. coli pili adhesin 32.2 kDa MW is toxic to male reproductive system. Samples consisted of spermatozoa of 30 guinea pigs divided into three groups: control, immunized with E. coli pili adhesin 32.2 kDa MW protein, and transurethral infected E. coli. Observations of sperm motility, vitality and morphology were performed under a microscope. MDA levels and sperm DNA damage were measured by a spectrophotometer and comet assay method and observed using a fluorescent microscope. There was no difference between control and immunization group of E. coli pili adhesin in motility (p=0.499), vitality (p=0.817) and morphology (p=0.176); between control and transuretral infection groups in motility (p=0.000), vitality (p=0.000) and morphology (p=0.000); and between control and both treatment groups in motility (p=0.001), vitality (p=0,000) and morphology (p=0.000). Histologic analysis showed E. coli pili adhesin of 32.2 kDa MW immunization group did not suffer from testicular tissue damage, while the positive group showed a deterioration of seminiferous tubular cells. MDA levels differed between immunization group E. coli pili, transurethral infection group, and control (p=0.024) and between transurethral and control (p=0.007) groups. However, between control and immunized group with E. coli pili protein showed no difference (p=0.251). DNA damage differed (p=0.000) between immunized group with E. coli pili, transurethral infection and control group; between control and transurethral infected group (p=0.000); and between transurethral infection group and E. coli pili protein immunization group (p=0.000). However, between control and E. coli pili immunization group showed no difference (p=0.600). In conclusion, E. coli pili adhesin 32.2 kDa MW protein is not toxic for sperm quality and the quality of sperm molecules.

Training and Mentoring of Probiotic Milk Fermentation Technology Development in Purwodadi Village, Purwodadi Sub-District, Pasuruan Achmad Toto Poernomo; Asri Darmawati; Isnaeni Isnaeni; Sudjarwo Sudjarwo
Warta Pengabdian Vol 15 No 2 (2021): Warta Pengabdian
Publisher : LP2M Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/wrtp.v15i2.20720

Purwodadi sub-district has a surplus of dairy milk with a production of 25 tons of milk per day per KUD. There are 4 milk industry in Purwodadi sub-district as suppliers of PT Nestle. The problem is, dairy products are not entirely absorbed by the market, so it is necessary to look for breakthroughs in the distribution of dairy products that have an average price of IDR 8,000-13,000 per liter. Diversification of milk into fermented milk products will increase prices by five times, so that it is expected to increase people's income, because not only is the product variant increasing, but the market is also increasing. The result of the training shows that all participants experience a significant increase in knowledge, indicated by higher post-test result compared to the pre-test result. Another result known is that this probiotic-fermented milk can be used as a medium for a source of economic improvement for the family. This is proven when yogurt made by the community of Purwodadi has been sold among them as an additional drink during community meetings. Apart from that, this training will create a climate that enables the potential of the Youth Organization (Karang Taruna) and Family Welfare Program (PKK) to develop, namely to build group creative power by encouraging, motivating, and raising awareness of the potential of the village and efforts to develop it.

Screening and Identification of Fibrinolitic Bacteria from Tempeh Muhammad Hakim Rafiga; Achmad Toto Poernomo; Sudjarwo Sudjarwo
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Tempe is a food that is well known by indonesian people. The purpose of this study was to obtain bacteria with the largest fibrinolytic activity from Tempe. This research sample was obtained from four different markets in Surabaya area. Proteolytic activity of bacteria is examined using Skim Milk Agar media. Followed by fibrinolytic examination on the fibrin plate media. Identification of bacteria is carried out by gram staining method and 16S rRNA method. The sample is prepared by mixing the sample with a normal saline solution until 10-7 dilution is obtained. Then the bacterial suspension is spread on SMA media then incubated at 37°C for 24 hours. The bacterial suspension is spread onSMA media then incubated at 37°C for 24 hours. The selection of fibrinolytic protease producing bacteria was carried out using Skim Milk Agar (SMA) and Fibrin Agar (FA) are characterized by the presence of clear zones around them. Bacteria that produce fibrinolytic activity are then cultured in the Nutrrient Agar medium. The bacteria was then tested for fibrinolytic activity on the Fibrin Plate media in incubation at 37°C for 24 hours. Positive bacteria are calculated by measuring the diameter of clear zones. Bacteria with sample code T2.2 produce the largest fibrinolytic index. T2.2 bacteria are then microscopically characterized, macroscopically, and bacterial isolates showing fibrinolytic activities were identified using Polymerase Chain Reaction (PCR) targeting their 16S rRNA gene. T2.2 bacteria is thought to be Stenotrophomonas maltophilia with a percentage similarity 96%.

Validasi Metode Spektrofotometri UV Untuk Penetapan Kadar Kolkisin Dalam Infus Kembang Sungsang Muhammad Muslich; Isnaeni Isnaeni; Sudjarwo Sudjarwo
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Validation of colchicine analysis methods in herbal infusions has been carried out to facilitate quality control of raw materials or simplicia. The validation parameters of the method set include selectivity, linearity, precision, and accuracy, which are included in category I. In this study, the raw material was prepared from the leaves of Gloriosa superba Linn. infusion made. The results of the selectivity test with colchicine as a standard provide uptake at a chosen wavelength of 340.2 nm. Linearity test results show the equation y = 0.0303x + 0.0131 with r = 0.9998> r Table for n = 6, ie 0.917 for p <0.01 and 0.811 for p = <0.05, the value of Vxo is 1 15%. In the precision test, the coefficient of variation is 0.54% and the accuracy test gives an average recovery of 92.81% ± 4.16%. Determination of colchicine in the infusion preparation was carried out as many as three replications. The results showed that the content of colchicine in raw materials was 0.3152% ± 1.99%, while the colchicine content in herbal infusion preparations was 0.483 ± 4.47%.

Validation Of Spectrophotometry-Visble Method On The Determination Of Borax Levels In Meatballs Sudjarwo Sudjarwo; Poedjiarti S; Angerina N
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 2 (2021): December
Publisher : Universitas Airlangga

Borax, in illictic additive substance, is added on certain food product as a e preservative and rubbery. Therefore, the determination of borax in the food product such as meatball is very impotant in view of meatball is a food product often consumed by community. Vis- Spectrophotometric method with curcumine 0.125% as a reagent and glacial acetic acid-sulphuric acid has been used for determination of borax in this research. Curcumin reagent was selected because sensitivity of the method and the reproducibility of the results are affected by quality of the reagent other than rigorous observance of the reaction conditions (temperature, time, reagent quantities). Glacial acetic acid- sulphuric acid was used to create acid condition, so that curcumin and boron form a violetred 2:1 complex called rosocyanin. The optimum result was obtained when 1/. ml solution of 0.125% curcuumin and 1.0 ml concentrated sulphuric acid were added and the absorbance was measured after 70 minutes at 547 nm. The results showed linear regression y = 1.3127x – 0.0994, r = 0.9690 > r table (n = 5) is 0.878 and p = 0.007 (p< 0.01) and Vxo is was 15,53%. The detection limit and quantitation limit were 9.7.10-4 ppm and 2.94. 10-3 ppm respectively. The recovery and coefficient variation were 47.56%±3,92%. Determination of borax in three meatball samples which were taken from a location in Surabaya showed that the sample contained borax with concentration of 0.0205; 0.0151; 0.0210 (% w/w) respectively.

PENGARUH ESCHERICHIA COLI DAN GRANULOSIT TERHADAP KADAR REACTIVE OXYGEN SPECIES SECARA IN VITRO Sukarjati; Doddy M. Soebady; Aucky Hinting; Sudjarwo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/268

In humans, male genital tract infection has been recognized as one of the causes of infertility. Indicators of the occurrence of genital tract infection are the presence of bacteria during semen culture (bacteriospermia) and the finding of leucocyte of more than 1 million/ml semen (leucotytospermia). Escherichia coli (E. coli) is the most common cause of prostatitis and epididymitis. The objective of this study was to determine the effects of E. coli and granulocytes on Reactive Oxygen Species (ROS) level in vitro. This study comprised of two experiments. In the experiment 1, sperm was incubated with E. coli, and the experiment 2, the sperm was incubated with granulocyte. In those experiments, ROS levels were observed. Spermatozoa were obtained from donor with normal spermatozoa according to WHO (1999). Escherichia coli was obtained by culturing the semen of infertile males. Granulocytes were obtained from donors’ blood. Sperm preparation was made by using Percoll gradient column method. Granulocyte isolation used Histopaque 1077 and 1119. ROS level was detected by means of chemiluminescence method with beta counter device. The result of this study showed that in vitro E. coli had the effect on ROS level, both stimulated by peroxidase (p = 0.000) and PMA (p = 0.006). Granulocyte had effect on ROS level. In peroxidase-stimulated ROS level, there was the effect between spermatozoa and granulocyte-incubated sperm (p = 0.000), granulocyte-incubated sperm and granulocyte (p = 0.002), and sperm and granulocyte (p = 0.000). In PMA-stimulated ROS level, there was effect between sperm and granulocyte-incubated sperm (p = 0.000), sperm and granulocyte (p = 0.000), granulocyte-incubated sperm and granulocyte (p = 0.000). In conclusion, under in vitro experiment, sperm incubated with E. coli and sperm incubated with granulocyte had the effect on the level of the ROS.

Co-Authors Achmad Toto Poernomo Afif Nurul Hidayati Angerina N Ashon Sa’adi Asri Darmawati Aucky Hinting Aucky Hinting Budi Utomo Dasrul Dasrul Diah Mira Indaramaya Didik Rio Pambudi Doddy M. Soebady Dwi Murtiastutik Emma Hidayati Sasmito Eni Setyawati Febri Annuryanti Hanifa Rahma Putri Hardjopranjot Hardjopranjot Irvan Ipandi Isnaeni Kurniawan, Hanif Lidya Tumewu Mahaputra L Marcellino Rudyanto Muhammad Hakim Rafiga Muhammad Muslich Noor Cholies Zaini Pepy Dwi Endraswari Poedjiarti S Purwitasari, Neny Rahmadewi Rice Disi Oktarina Riska Rovitasari Risma Margaretha Sinaga Sawitri Setyo Prihatiningtyas Siti Husnul Khatimah Suciati Suciati Sukarjati Sukarjati Sukarjati Susie Amilah Toto Poernomo Tutiek Purwanti Wiwied Ekasari Yanu Andhiarto ZAINAL ABIDIN

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