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All Journal Berkala Kedokteran Jurnal Surya Medika Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat JURNAL PENDIDIKAN MIPA Pharmacoscript Jurnal Farmamedika (Pharmamedica Journal) Jurnal Riset Kefarmasian Indonesia Science Midwifery International Journal of Clinical Inventions and Medical Sciences (IJCIMS) Reswara: Jurnal Pengabdian Kepada Masyarakat Lumbung Farmasi : Jurnal Ilmu Kefarmasian Indonesia Berdaya Hydrogen: Jurnal Kependidikan Kimia Jurnal Sains dan Kesehatan Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Dinamika Kesehatan: Jurnal Kebidanan dan Keperawatan Journal of Pharmaceutical Care and Sciences Jurnal Matematika Dan Ilmu Pengetahuan Alam LLDikti Wilayah 1 (JUMPA) Jurnal Rumpun Ilmu Kesehatan Innovative: Journal Of Social Science Research Sains Medisina Journal of Advances in Medicine and Pharmaceutical Sciences Juragan (Jurnal Agroteknologi) JFARM (Jurnal Farmasi)
Kunti Nastiti
Universitas Sari Mulia
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Journal : Journal of Advances in Medicine and Pharmaceutical Sciences

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Thin Layer Chromatography of Secondary Metabolite Profiles and Determination of Total Flavonoid Levels of the Sawi Langit Extract that Growing on Peatlands Feliya Carolina; Kunti Nastiti; Muhammad Rizali
Journal of Advances in Medicine and Pharmaceutical Sciences Vol 1 No 1: May 2022
Publisher : Lamintang Education and Training Centre, in collaboration with the International Association of Educators, Scientists, Technologists, and Engineers (IA-ESTE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36079/lamintang.jamaps-0101.427

Abstract

Peatland is a type of soil with a high organic content, typically more than 65 percent organic matter, high acidity, and low nutrient content. Vernonia cinerea, also known as Sawi Langit (Vernonia cinerea L.) in Indonesia, is one of the nutritious plants. The community uses this herb to treat fevers, wounds, discomfort, and ulcers. The secondary metabolite concentration of plants in peatlands is affected by nutrients such as carbon, nitrogen, and pH. Find out the secondary metabolite content of Sawi Langit leaf extract and the total flavonoid levels that grown on peatlands. The Rf value was estimated after the descriptive observational approach was obtained from the Thin Layer Chromatography (TLC) profile of secondary metabolites with spray reagents, and the quantitative data was determined by UV-Vis spectrophotometry. Sawi Langit plants were found growing on peatlands with a pH of 3.77, N-total of 1.51%, and C-Organic of 4.16%. The best eluent for separating compounds is chloroform: methanol: water (2:5:3) for flavonoid compounds, ethyl acetate: methanol: water (16:1:2) for alkaloid compounds, chloroform: methanol (9:1) for steroid compounds, n-hexane: ethyl acetate (12:8) for terpenoid compounds, ethyl acetic: chloroform: acetic acid (15:5:2) for tannin compounds, and chloroform: ethanol: water (10:6:1) for saponin compounds. Sawi langit leaf extract has a total flavonoid concentration of 5.527 mgQE (Quersetin Equivalent)/g. Flavonoid, alkaloids, steroids, terpenoids, tannins, and saponins compounds were found in the TLC profile of Sawi Langit leaf extract, and the total flavonoid content of the leaf extract of Sawi Langit was 5.527 mgQE (Quersetin Equivalent)/g.
Co-Authors Adi Rahmadi Ana Maulana Ana Muliana Arif Subekti Citra Agustiani Kusuma Dahlia Syahrina Darini Kurniawati Darini Kurniawati Darini Kurniawati Dede Mahdiyah Deni Setiawan Desiya Ramayanti Azhara Diah Aulia Rosanti Dian Ekowati Dyan Fitri Nugraha Dyan Fitri Nugraha Dyan Fitri Nugraha Dyan Fitri Nugraha Faisal Rahman Feliya Carolina Firda Nirmalasari Fitri Yuliana Ghina Zhahera Ihda Syifa El Rahma Juliance Yolanda Putri Mambang Maria Dwi Ayu Listiawati Melviani Melviani Melviani Melviani Melviani Melviani Melviani Melviani Melviani Mia Audina Mia Audina Muhammad Noor N Noval N Novia Ni Ketut Linda Puspa Yani Noval Noval Nur Syifa Nurul Hidayah Putri Vidiasari Darsono Rahmadani Rahmadani Rahmayani Rahmayani Rizali, Muhammad Samsul Hadi Setia Budi Setia Budi Siti Malahayati Siti Malahayati Sukmana, M. Laily Qadry Tuti Alawiyah Umi Kalsum Vita Mayasari Witri Alya Yanti Saubari Yanti Saubari