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All Journal Jurnal Kesehatan Folia Medica Indonesiana Biota BIOTROPIC The Journal of Tropical Biology Jurnal Riset Biologi dan Aplikasinya Nusantara Journal of Community Engagement Engagement: Jurnal Pengabdian Kepada Masyarakat

Moch. Irfan Hadi

Biology Departement, Faculty Science And Technologi, UIN Sunan Ampel Surabaya

Author-ID : 2602048

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Optimalisasi Pengetahuan Tentang Menstruasi Pada Siswa Putri Kelas 5 dan 6 SDN Janti 1 Waru Sidoarjo Dengan Pendekatan PAR Mei lina Fitri Kumalasari; Nova Lusiana; Estri Kusumawati; Dedy Suprayogi; Moch. Irfan Hadi
Nusantara Journal of Community Engagement Vol. 1 No. 1 (2020): MARET
Publisher : Lembaga Penelitian dan Pengabdian (LP2M) Sekolah Tinggi Agama Islam Cendekia Insani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2020/njce.v1i1.3664

Anak perempuan yang mengalami menarche (menstruasi pertama) pada usia yang muda merasa belum siap untuk menghadapi menstruasi pertamanya sehingga dapat menimbulkan dampak takut, bingung, minder, merasa tidak normal dan panik. Pengabdian masyarakat ini bertujuan untuk optimalisasi pengetahuan tentang menstruasi pada siswa putri kelas 5 dan 6 di SDN Janti 1. Metode kegiatan yang dilakukan adalah dengan pendekatan PAR. Didapatkan hasil bahwa dari 89 siswa putri, terdapat 40 siswa putri (44,94%) yang sudah mendapatkan menstruasi. Siswa putri tersebut mengalami menarche pada usia 10 sampai dengan 12 tahun. Terdapat 22 siswa putri (55%) yang mengalami dismenorea dan belum mengetahui apa yang harus dilakukan apabila mengalami keluhan tersebut. Materi diberikan dengan media powerpoint dan video. Siswa putri kelas 5 dan 6 SDN Janti 1 aktif dalam kegiatan dengan memberikan pertanyaan-pertanyaan dan saling berbagi pengalaman.

Analisis Kadar Glukosa, Hemoglobin, dan Kolesterol Mencit (Mus Musculus) Setelah Diinduksi Aspartam Esti Tyastirin; Risa Purnamasari; Irul Hidayati; Eva Agustina; Moch. Irfan Hadi; Nova Lusiana; Mei Lina Fitri Kumalasari
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Aspartame has a lower calorie rating than other sweeteners. for example, cyclamate, lactose, saccharin, fructose and maltosa. Although it has low calorie, aspartame have 60 times sweeter than sugar (sucrose). Induction of aspartame can cause elevated levels of blood glucose and damage to the pancreatic β cells of mice. The purpose of this study was to analyze the dosing of aspartame levels; 13 mg/Kg body weight, 39 mg/Kg and 78 mg/Kg and those effect to the levels of glucose, cholesterol and haemoglobin of female mice (Mus musculus). The methode of research laboratory experiments using a complete randomized design (RAL). This study was using 24 mice with 4 treatment groups. Mice were given aspartame during surgical treatment, mice were made to perform measurement of Hb levels, glucose levels and cholesterol levels of neonatal mice. Data analysis using the Kruskal Wallis because Gaussian data but not homogeneous. The results showed an increase in the levels of glucose and cholesterol levels, but statistically there is no difference in glucose levels (p = 0,087), Hb (p = 0,899) and cholesterol (p = 0,168) in mice (Mus musculus) who were given variations of the dose of aspartame.

Identifikasi Senyawa Aktif dari Ekstrak Daun Jambu Air (Syzygium aqueum) dengan Perbandingan Beberapa Pelarut pada Metode Maserasi Eva Agustina; Funsu Andiarna; Nova Lusiana; Risa Purnamasari; Moch. Irfan Hadi
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Daun jambu air (Syzygium aqueum) dikenal sebagai salah satu obat tradisional, untuk menyembuhkan beberapa infeksi akibat antigen berupa bakteri. Penelitian ini bertujuan untuk mengetahui kandungan senyawa aktif yang terdapat dalam daun jambu air dengan beberapa pelarut pada proses maserasi. Metode ekstraksi yang digunakan dalam penelitian ini adalah ekstraksi dingin berupa maserasi dengan pemilihan pelarut yakni metanol (CH3OH), etil asetat dan n-heksana. Uji fitokimia yang dilakukan adalah uji saponin, flavonoid, alkaloid dan triterpenoid/steroid. Dari uji yang dilakukan diketahui sampel ekstrak daun jambu air dengan pelarut metanol positif mengandung beberapa senyawa aktif berupa flavonoid, saponin, alkaloid dan triterpenoid. Ekstrak daun jambu air dengan pelarut etil asetat dan n-heksana positif mengandung beberapa senyawa aktif berupa flavonoid, alkaloid dan triterpenoid. Pelarut yang paling bagus untuk mendapatkan banyak senyawa aktif adalah metanol. Kata kunci : Jambu air (Syzygium aqueum), maserasi, senyawa aktif

Analisis Kariometric Akibat Pemberian Variasi Dosis Aspartam pada Perkembangan Fetus Mencit (Mus Musculus L.) Esti Novi Andyarini; Nova Lusiana; Ika Mustika; Estri Kusumawati; Sri Hidayati; Moch Irfan Hadi; Risa Purnamasari; Dedy Suprayogi
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Aspartame is safe for consumption according to the prescribed ADI, but this artificial sweetener is not nutritious or does not contain calories so it is not recommended to be consumed during pregnancy. This study was to analyze cariometric effect of aspartame doses in the development of fetal mice (Mus muculus L.). The type of research used was experimental laboratory research on 24 adult mice with 4 treatment groups, control, aspartame dose 13 mg / Kg BB, 39 mg / Kg BB and 78 mg / Kg BB. Data was analyzed using SPSS 16 with one way ANOVA. Based on the results of the study there were changes in the number and weight of the fetus due to aspartame doses, there was no change in fetal length due of aspartame dose variations. Consumption of aspartame during pregnancy should be avoided because it can cause teratogenic effects. For further research, it is necessary to observe the morphology and histology of the fetus which is expressed by aspartame to determine the types of congenital abnormalities.

Kontaminasi Residu Pestisida Organofosfat di Dalam Tanaman Holtikultura Widia Purnamasari; Moch Irfan Hadi; Eva Agustina
Biotropic : The Journal of Tropical Biology Vol. 4 No. 2 (2020): Biotropic, Volume 4 Nomor 2, 2020
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2020.4.2.98-110

Excessive use of organophosphate pesticides is becoming more common these days. This can affect the level of pesticide residues in farmers' crops. Excessive spraying of pesticides can result in environmental pollution both small and large scale. If excessive exposure to these pesticides can cause damage to the ecosystem. The purpose of this study was to determine the level of organophosphate pesticide residues found in horticulture plants. This research method is a systematic review, by analyzing various studies regarding the contamination of organophosphate pesticide residues in horticultural crops. There are 20 studies from within the country. The results obtained in the form of levels of pesticide residues that are still below the threshold of the BMR (Maximum Residue Limit) of pesticides determined by SNI. Two other studies show that no pesticide residue content was detected. Conclusion: This shows that the sample tested is still safe for consumption. Socialization and education about the doses of pesticide use and how to reduce levels of pesticides in horticultural crops are needed to reduce the risk of exposure to pesticides

Nematoda Parasit Pada Bunomys spp. di Pegunungan Mekongga, Sulawesi Tenggara Muhammad Falikhul Musyaffa'; Kartika Dewi; Mochammad Irfan Hadi
Biotropic : The Journal of Tropical Biology Vol. 5 No. 2 (2021): Biotropic, Volume 5 Nomor 2, 2021
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2021.5.2.58-67

Study on the nematode parasite species of Bunomys spp. from Mekongga Mountain, Southeast Sulawesi was conducted on deposited specimens in Museum Zoologicum Bogoriense, Zoology Division, Research Center of Biology-LIPI. Bunomys is endemic genus murine rodent in Sulawesi. This study aims to determine the types of nematodes in endemic Muridae rodent, the results of which can help the government in developing disease management plans. The nematodes identified in this study were Subulura andersoni (host: B. andrewsi), Syphacia rifaii (host: B. chrysocomus), Cyclodostomum purvisi (host: B. penitus), Heterakis spumosa (host: B. chrysocomus). Except for Sy. muris, all of the recorded nematodes have worldwide distribution. Morphology and taxonomy of nematodes will be discussed in this paper

HUBUNGAN TINGKAT STRES PSIKOLOGIS DENGAN SIKLUS MENSTRUASI PADA MAHASISWA Mei Lina Fitri Kumalasari; Moch Irfan Hadi; Misbakhul Munir
Jurnal Kesehatan Vol 12 No 2 (2019): JURNAL KESEHATAN
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/kesehatan.v12i2.10842

The menstrual cycle is the distance between the first day of menstruation and the first period of the next menstruation. One of the faktors that influence the menstrual cycle is stres. Stres can stimulate the release of the hormone cortisol, which is a product of glucocorticoid adrenal cortex. This hormone affects the amount of progesterone in the body. This hormonal imbalance will cause changes in the menstrual cycle. The purpose of this study was to analyze the relationship between stres levels and the menstrual cycle of UIN Sunan Ampel Surabaya students. This research was an observational analytic study with a sampling design using simple random sampling with a total of 30 respondents. Data collection techniques use the DASS 42 questionnaire to measure stres levels and the menstrual cycle pattern questionnaire to measure the menstrual cycle. Data analysis using Fisher's exacs test and the results obtained p = 0.031, so it means that there is a relationship between the level of stres with the menstrual cycl.ABSTRAKSiklus menstruasi adalah jarak antara hari pertama menstruasi dengan hari pertama pada menstruasi berikutnya. Hal ini dapat dipengaruhi oleh stress yang merangsang pengeluaran hormone kortisol yang merupakan produk dari glukokortiroid korteks adrenal yang disintesis di zona fasikulata. Hormon ini mempengaruhi jumlah hormone progesterone di dalam tubuh. Ketidakseimbangan hormon ini akan menyebabkan perubahan siklus menstruasi. Tujuan dari penelitian ini adalah untuk mengetahui hubungan antara tingkat stres dengan siklus menstruasi mahasiswai UIN Sunan Ampel Surabaya. Penelitian ini merupakan penelitian observasional analitik dengan desain pengambilan sampel menggunakan simple random sampling dengan jumlah 30 responden. Tehnik pengumpulan data menggunakan kuesioner DASS 42 untuk mengukur tingkat stres dan kuesioner pola siklus menstruasi untuk mengukur siklus menstruasi. Analisis data menggunakan Fisher’s exacs test dan didapatkan hasil nilai p=0.031. Hal ini berarti menunjukkan bahwa ada hubungan hubungan antara tingkat stres dengan siklus menstruasi. Penelitian selanjutnya sebaiknya menambahkan variabel lain yang mempengarusi siklus menstruasi dan menamba jumlah sampel agar diperoleh data yang lebih baik.

Optimization of PCR Protocol for the Identification of Yeasts Isolated from Apis mellifera Honeycomb based on the ITS rDNA Ayudya Fitri Arifa; Nirmala Fitria Firdhausi; Irul Hidayati; Yuanita Rachmawati; Moch. Irfan Hadi
Biota Vol 8 No 2 (2022): Jurnal Biota 2022
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v8i2.10181

Yeast is a microorganism that can be found in honeycomb. Yeast identification is a process to find and identify new species. One of which is molecular identification of yeast with rDNA sequences in the ITS region. Before carrying out molecular identification, it is necessary to optimize yeast DNA amplification methods to obtain good DNA sequences that ease the yeast identification process. The purpose of this study was to discover the optimum PCR (Polymerase Chain Reaction)protocols for the identification of yeasts isolated from Apis mellifera honeycomb based on the ITS rDNA. This study used 3 PCR (Polymerase Chain Reaction) protocols, i.e., from Kanti et al. (2018), Ediningsari (2008), and Maulana (2011). This results study shows that the optimum PCR protocol was from Maulana (2011), which produced clear and whole DNA fragment luminescences.

Viral Load And Cd4+ among Hiv/Aids Patients Receiving Antiretroviral Therapy In Jayawijaya District, Papua Province, Indonesia Mirna Widiyanti; Moch. Irfan Hadi; Setyo Adiningsih; Evi Iriani Natalia; Dedi Ananta Purba
Folia Medica Indonesiana Vol. 58 No. 1 (2022): March
Publisher : Universitas Airlangga

Hightlight:Clinical conditions, CD4+ cell counts, and the viral copies number in the blood for AIDS/HIV were given antiretroviral therapy.The profile of CD4+ levels and plasma viral load in HIV patients receiving antiretroviral therapyThe lower CD4+ cell counts and higher viral loads happen in HIV-infected’s men. Abstract:Highly active antiretroviral therapy (HAART) is expected to reduce human immunodeficiency virus (HIV) morbidity and mortality. Antiretroviral therapy in HIV patients is given based on clinical conditions, CD4+ cell counts, and the number of viral copies in the blood. This study aimed to determine the profile of CD4+ levels and plasma viral load in HIV patients receiving antiretroviral therapy. This was a cross-sectional study conducted within six months at Voluntary Counseling and Testing (VCT) in Jayawijaya Hospital, Papua, Indonesia. The CD4+ levels were measured using CD4+ counter and viral plasma was checked using Polymerase Chain Reaction (PCR) for 90 patients. The results showed more female patients had a CD4+ level <200 cells/mm3, a higher number of copies of the virus in the blood plasma, and stages of disease 3 and 4. Statistically, there was a significant relationship between CD4+ levels and gender with a p-value = 0.00. HIV-infected males were more likely to have lower CD4+ cell counts and higher viral loads than females.

Optimization of Various ITS rDNA Amplification Protocol of Yeast Isolated from Giant Honey Beehives (Apis dorsata) Chumaidatul Choiriyah, S.Si; Nirmala Fitria Firdhausi; Esti Tyastirin; Yuanita Rachmawati; Moch. Irfan Hadi
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p80-87

Indonesia is a country with high variability of microorganisms, including bacteria, yeast, and fungi. Yeast isolates could be isolated from the honeycomb of Apis dorsata. Molecular approaches were used to identify yeast using ribosomal DNA gene sequences, called the ITS gene. The optimum condition for DNA extractions and amplifications are needed for the successfully of molecular identification. Therefore, it is necessary to optimize the DNA extraction and amplification of several protocols to obtain good identification results. This study aimed to compare the effects of DNA extraction with various temperatures and different amplification protocols. LIPI reference DNA extraction protocol with the boiling method and variations in incubation time of 10, 15, and 20 minutes at a temperature of 98° C. Meanwhile, for the amplification of yeast DNA using a variety of different amplification protocols. The results showed the optimal time of incubation was 10 minutes in K1 isolates with DNA purity of 1.896. meanwhile, for isolates K2, K3, and K4 each with a purity of 2.246, 2.335, and 1.748. optimal DNA amplification results were indicated by the presense of DNA bands for each sample K1, K2, K3, and K4, namely 503, 542, 492, and 526 bp. In this study, it can be concluded that the optimal incubation time for the extraction process is 10 minutes. In addition, the optimal amplification protocol was shown in the DNA bands in all sample.

Co-Authors Abdul Azis Abdul Muhid Asri Sawiji Ayudya Fitri Arifa Chumaidatul Choiriyah, S.Si Dedi Ananta Purba Dedy Suprayogi Dedy Suprayogi Dian Tugu Warsito Taufiq Esti Novi Andyarini Esti Novi Andyarini Esti Tyastirin Esti Tyastirin Estri Kusumawati Estri Kusumawati Eva Agustina Eva Agustina Evi Iriani Natalia Funsu Andiarna Ika Mustika Ika Mustika Ika Mustika Irul Hidayati Irul Hidayati Kartika Dewi M. Chusnan Ma'arif Mei lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mirna Widiyanti Misbakhul Munir Muhammad Falikhul Musyaffa' Nirmala Fitria Firdhausi Nirmala Fitria Firdhausi Nova Lusiana Nova Lusiana Nova Lusiana Nur Maulida Safitri Puji Maryono Risa Purnamasari Risa Purnamasari Robayanto Robayanto Setyo Adiningsih Sri Hidayati Widia Purnamasari Wiga Alif Violando Yuanita Rachmawati, Yuanita

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