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Aktivitas Antibakteri Belimbing Manis (Averrhoa carambola L.) terhadap Methicillin Resistant Staphylococcus aureus (MRSA) Yunita Yunita; Risa Nursanty
Jurnal Bioleuser Vol 3, No 2 (2019): Agustus 2019
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, University of Syiah Ku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/j. bioleuser.v3i2.18821

Abstract

Indonesia memiliki keanekaragaman tumbuhan yang sangat tinggi. Banyak di antara tumbuhan tersebut memiliki khasiat sebagai obat, misalnya belimbing manis (Averrhoa carambola L.). Secara tradisional, belimbing manis telah digunakan untuk mengobati berbagai macam penyakit. Kemampuan ini diduga karena belimbing mengandung senyawa yang berpotensi untuk menghambat pertumbuhan bakteri. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak metanol daun dan kulit batang belimbing terhadap pertumbuhan Methicillin Resistant Staphylococcus aureus (MRSA). Ekstraksi tumbuhan dilakukan dengan metode maserasi sementara aktivitas anti bakteri dilakukan dengan metode Kirby-Bauer. Pada uji aktivitas antibakteri menggunakan tiga (3) konsentrasi ekstrak yang berbeda, yaitu: 25, 50, dan 70%. Sebagai negatif control digunakan metanol dan sebagai positif control digunakan linezoid. Hasil pengamatan menunjukkan bahwa ekstraksi batang pada konsentrasi 75% memiliki kemampuan daya hambat yang terbesar terhadap pertumbuhan MRSA
Isolasi dan Karakterisasi Bakteri pada Ikan Kembung (Rastrelliger sp.) Asin Berkitosan Diana Agustina; Cut Yulvizar; Risa Nursanty
Biospecies Vol. 6 No. 1 (2013): Januari 2013
Publisher : Universitas Jambi

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Abstract

Kitosan memiliki sifat antibakteri sehingga dapat dimanfaatkan sebagai bahan pengawet ikan. Penelitian ini dilakukan di Laboratorium Mikrobiologi Universitas Syiah Kuala, Banda Aceh dari bulan Mei sampai Agustus 2012, untuk mengisolasi dan mengkarakterisasi  bakteri pada ikan kembung (Rastrelliger sp.) asin berkitosan. Metode yang digunakan dalam penelitian ini deskriptif. Dari hasil isolasi didapat enam isolat yang memiliki morfologi koloni dan sel yang berbeda. Isolat yang memiliki bentuk bundar (50%), elevasi cembung (33,3%), tepian licin (50%) warna kuning (66,7%), sel bakteri gram positif (66,7%), bentuk sel bulat (100%), tidak berspora (66,7%) dan bersifat motil (66,7%).
INHIBITION TEST OF METHANOL EXTRACT FROM SOURSOP LEAF (Annona muricata Linn.) AGAINST Streptococcus mutans BACTERIA* Raudhatul Jannah; Muhammad Ali Husni; Risa Nursanty
Jurnal Natural Volume 17, Number 1, March 2017
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.595 KB) | DOI: 10.24815/jn.v17i1.6823

Abstract

Abstract. Dental caries is a disease with a high prevalence of caries in Indonesia is caused by the bacterium Streptococcus mutans. The leaves of the soursop (Annona muricata Linn.) is one of the herbs that can remove dental plaque. This study aims to determine the chemical content and measure the inhibitory antibacterial soursop leaft methanol extractin inhibiting the growth of Streptococcus mutans bacteria. Phytochemical screening stated that the leaf of the soursop contains flavonoids, alkaloids, tannins, steroids, glycosides and saponins. Test of inhibition were measured using paper disc diffusion method with variation concentrations of 5, 10, 15 20, and 25%, positive control is tetrasilkin 30 µg, and a negative control is methanol. Antibacterial test results showed that the methanol extract of soursop leaves have antibacterial activity against Streptococcus mutans at concentrations of 5, 10, 15, 20 and 25% with inhibitory diameter of 9,1; 10,57; 11,53; 12,01 and 13,75 mm respectively. Keywords: Soursop leaves, Dental caries, Streptococcus mutans, Methanol.*Judul ini telah dipresentasikan pada Seminar Nasional: Indonesian Students Conference on Science and Mathematics (ISCSM) 11-12 November 2015, Banda Aceh Indonesia
Potency Study of N-Hexane Extracts of Black Plum (Syzygium cumini (L.) Skeels) Intheinhibitation of Growth Salmonella typhi and Candida sp. Saida Rasnovi; Risa Nursanty
Jurnal Natural Volume 15, Number 1, March 2015
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.345 KB)

Abstract

N-hexane extracts of the bark from black plum(Syzygium cumini (L.) Skeels) showed activity againtSalmonella typhi. Meanwhile,  the result showed that N-hexane was not able to inhibit the  growth of Candida sp. The research was done at Microbiology laboratory, Biology Department Faculty of Mathemathics andScience,  Syiah Kuala University. The bark of black plum were collected from Aceh Besar District. The extraction method  for black plum used maceration with N-hexane and than concentrated in rotary evaporator. The disc diffusion method used to screen the antimicrobial activity with 20 %, 30 %, 40 % dan 50 % concentration.
Potency Study of N-Hexane Extracts of Black Plum (Syzygium cumini (L.) Skeels) in the Inhibitation Growth of Salmonella typhi and Candida sp. Saida Rasnovi; Risa Nursanty
Jurnal Natural Volume 14, Number 1, March 2014
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.192 KB)

Abstract

N-hexane extracts of the bark from black plum (Syzygium cumini (L.) Skeels) showed activity against Salmonella typhi. Meanwhile, the result showed that N-hexane was not able to inhibit the growth of  Candida sp. The research was done at Microbiology laboratory, Biology Department Faculty of Mathematics and Science,  Syiah Kuala University. The barks of black plum were collected from Aceh Besar District. The extraction method for black plum used maceration with N-hexane and than concentrated in rotary evaporator. The disc diffusion method used to screen the antimicrobial activity with 20 %, 30 %, 40 % dan 50 % concentration.