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Aktivitas Ekstrak Etanol Daun Alpukat Terhadap Zat Nefrotoksik Ginjal Tikus (ACTIVITIES STUDY OF ETHANOL EXTRACTS OF AVOCADO LEAVES (PERSEA AMERICANA MILL) TO NEPHROTOXIC COMPOUND OF RAT’S KIDNEY) Ietje Wientarsih; Eva Harlina; Rini Madyastuti Purwono; Ikrar Trisnaning Hardi Utami
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Based on WHO’s data, about 80% peoples in the word use plant’s extract as herbal medicine. Avocadoleaves has used as herbal medicine which is works as diuretikum, reduce stone in renal, and cure sprue.The purpose of this research is to study activities of ethanol extract of avocado leaves to ethylene glycolinduction on rat’s kidney by histopatologically. This experiment was continuing Adha’s research (2009)with research design as follows: twenty male rats were divided into 4 groups, negative control group (KN),positive control group (KP), treated group I by ethanol extract of avocado leaves 100 mg/kg BW (E100), andtreated group 2 by 300 mg/kg BW (E300). Sampling kidney were fixed in BNF 10% for histopathologicalslide and stained by Hematoxylin-Eosin. The kidney histopathological changes were edema of glomerulus,and protein deposit in the lumen, hyalin droplet and necrotic tubules. The results showed that ethanolextract of avocado leaves can’t significantly to reduce glomerulus edema with percentage cases 31.9% and33.5% for E100 and E300 and tend not to reduce the occurrence of protein deposits in the lumen of thetubule. Ethanol extract of avocado leaves tend to reduce the incidence of tubular necrosis and it wassignificantly different from the KP group (p <0.05). It is caused by flavonoid in etanol extract of avocadoleaves works as diuretikum and antioxidant.
Aktivitas Diuretik dan Analisa Mineral Urin Perlakuan Ekstrak Tanaman Kumis Kucing (Orthosiphon Stamineus Benth) pada Tikus Jantan Madyastuti, Rini; Ietje wientarsih; Setyo Widodo; Erni H Purwaningsih; Eva Harlina
Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (683.912 KB) | DOI: 10.29244/avi.8.2.16-23

Abstract

Tanaman Kumis Kucing merupakan salah satu tanaman yang memiliki banyak keuntungan dan sudah digunakan sejak dahulu dalam upaya menjaga kesehatan. Tujuan dari penelitian ini adalah mengevaluasi aktivitas diuretik dari penggunaan ekstrak Kumis Kucing dan mengukur kadar kalium dan natrium dalam urin. Sebanyak 24 ekor tikus dibagi menjadi empat grup; kontrol negatif (akuades), kontrol positif (furosemide), dosis ekstrak 1 (250mg/kg BB) dan dosis ekstrak 2 (500mg/kg BB). Ekstrak diberikan secara peroral selama 7 hari. Aktivitas diuretik pada kelompok ekstrak dan kontrol positif pada jam pertama (P>0.05) dan jam keenam (P<0.05). Volume urin kumulatif dosis 1 mendekati furosemide. Pengukuran mineral urin menunjukkan hasil kehilangan mineral natrium dan kalium pada kelompok ekstrak lebih kecil dibandingkan kelompok referensi. Hasil dari penelitian ini menunjukkan bahwa dosis efektif ekstrak Kumis Kucing adalah 250 mg/kg BB pada jam ke-2 dan menunjukkan kehilangan mineral dalam urin yang lebih kecil.
Aktivitas Diuretik dan Analisa Mineral Urin Perlakuan Ekstrak Tanaman Kumis Kucing (Orthosiphon Stamineus Benth) pada Tikus Jantan Ietje wientarsih; Setyo Widodo; Erni H Purwaningsih; Eva Harlina; Rini Madyastuti
Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (683.912 KB) | DOI: 10.29244/avi.8.2.16-23

Abstract

Tanaman Kumis Kucing merupakan salah satu tanaman yang memiliki banyak keuntungan dan sudah digunakan sejak dahulu dalam upaya menjaga kesehatan. Tujuan dari penelitian ini adalah mengevaluasi aktivitas diuretik dari penggunaan ekstrak Kumis Kucing dan mengukur kadar kalium dan natrium dalam urin. Sebanyak 24 ekor tikus dibagi menjadi empat grup; kontrol negatif (akuades), kontrol positif (furosemide), dosis ekstrak 1 (250mg/kg BB) dan dosis ekstrak 2 (500mg/kg BB). Ekstrak diberikan secara peroral selama 7 hari. Aktivitas diuretik pada kelompok ekstrak dan kontrol positif pada jam pertama (P>0.05) dan jam keenam (P<0.05). Volume urin kumulatif dosis 1 mendekati furosemide. Pengukuran mineral urin menunjukkan hasil kehilangan mineral natrium dan kalium pada kelompok ekstrak lebih kecil dibandingkan kelompok referensi. Hasil dari penelitian ini menunjukkan bahwa dosis efektif ekstrak Kumis Kucing adalah 250 mg/kg BB pada jam ke-2 dan menunjukkan kehilangan mineral dalam urin yang lebih kecil.
Infusum Daun Alpukat Sebagai Inhibitor Kristalisasi Kalsium Oksalat pada Ginjal (THE AVOCADO LEAVES INFUSUM AS INHIBITOR ON RENAL CALCIUM OXALATE CRYSTALIZATION) Rini Madyastuti; Setyo Widodo; Ietje Wientarsih; Eva Harlina
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Urine crystal is a crystal nucleus which tend to form urine stone. The case of urine stone seems to beincreased every year. Crystallization could induce acute tubular necrosis which impact on renal dysfunction.The signs of this condition are high level of urea, creatinine and decrease glomerulus filtration rate. Theobjective of this research was to evaluate the effects of infusum Persea americana Mill as an inhibitorcrystallization which induced by ethylene glycol on white male rats. 20 male rats were divided into 4groups; K1 as negative group received only distilled water ad libitum, K2 as positive group receiveddistilled water containing ethylene glycol, K3 (dose 5%) and K4 (dose 10%) as treatment groups receivedwater containing ethylene glycol and avocado leaves infusion. Phytochemsitry screening of infusion avocadoleaves consisted of flavonoid, saponin, tanine and quinone. Result of analysis showed that the level ofureum and creatinine on K2 was higher than K3 and K4 group. The increased level could be inhibited byinfusion avocado leaves. The measurement of glomerular filtration rate in treatment groups wassignificantly different (p<0.05). Descriptive histopathology observation showed that renal lesio in grouptreatment (K3 and K4) were declined. Large crystal calcium oxalate on K2 group was observed by usingpolarized microscope, whereas small crystal calcium oxalate were seen in the infusion of avocado leavesgroups. These result showed the ability of infusion of avocado leaves as an inhibitor on the growth ofcrystallization calcium oxalate
PAT-3 In Vitro Growth Inhibition Activities of Natural (nCaIFN) and Recombinant (rCaIFN) Canine Interferons on Three Different Tumor-Derived Cell Lines Bambang Pontjo Priosoeryanto; Rizki Rostantinata; Eva Harlina; Lina Noviyanti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Recently, from many kinds of diseases one could be counted is a tumor disease. Tumor is a degenerative disease that involved many biological pathways within the host. A tumor or neoplasm can be defined as a disturbance of growth characterized by excessive, abnormal and uncontrolled proliferation of transformed or altered cell(s) at  one or more primary points within the host, and frequently at  one or more metastatic sites (Priosoeryanto, 1994).The treatment of tumor is mostly by medical surgery which usually combined with chemotherapeutic agent(s), unfortunately the using of chemotherapeutic agent can develop a seriously side effect to the treated-patients. Biological substances like interferon (IFN) known as anti-virus agent is also one of promising candidate for treating or preventing of tumor disorders.Feline and canine squamous cell carcinoma is one of tumor type that often found in cat and dog, and mostly develop to a very aggressive disorder. The using of in vitro cell culture is a way to avoid the use of live animals on the study of tumors especially the study of antitumor agent due to can mimic the in vivo condition.  The aim of the present study is to examine the growth inhibition activity of natural and recombinant canine interferon in order to find the suitable biological substances for combating tumor disorder especially in the field of veterinary medicine and also as an information for the development of tumor treatment in the human medical side.
PAT-8 Antiproliferation Activity of Keladi Tikus (Typhonium flagelliforme) Leaves Ethanol Extract on MCA- B1 and MCM-B2 Tumor – Derived Cell Lines In Vitro Riski Rostantinata; Bambang Pontjo Priosoeryanto; Eva Harlina; Waras Nurcholis; Lina Noviyanti; Rachmi Ridho
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

INTRODUCTIONTumor  is  a  degenerative disease  as  the second agent cause of death in human. This disease is caused by disturbances of cell’s growth that show alteration and uncontrolled of cells proliferation. The uncontrolled of cells proliferation is also accompanied by penetration of that cells into others tissue and develop on it. Removed of tumor is usually done by operation or chemotherapy. Alternative treatment that can be used is herbal treatment. Typhonium flagelliforme known as “Keladi Tikus” in Indonesia; is known has a chemopreventive effect (2). Other researchers also show flavonoid glucoside of T. flagelliforme (1) can induce the activity of apoptosis in colon cancer cells (5). The aim of this study is to examine the in vitro anti-proliferation activity of Typhonium flagelliforme leaves ethanol extract on MCA- B1and MCM-B2 cell line.
Evaluasi Radiografi terhadap Kepadatan Tulang Hasil Implan Scaffold 3D Bifasik Kalsium Fosfat Alginat pada Domba Lokal (Ovis aries) Dwi Utari Rahmiati; Gunanti Gunanti; Eva Harlina
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2021.22.2.285

Abstract

0 based on chages in size of defect and relative bone density, 2) implant response tissue based on relative muscle density peri implant. The BCP used in this study made from hydroxyapatite (HA) 70, tricalcium phosphate (TCP) 30 and alginate as porogen. The implant material made in 3D form and have diameter porous 200-400 ìm which similar size with the bone trabecula. This study used three local sheep (Ovis aries) male 1.5 years old, weight average 20 kg. The implant material is grown on the right sheep tibia. As a control, the left tibia is made defective with no implantable material. Evaluate osteogenesis and implant tissue responses using radiography and assess the grey values of a particular area. Osteogenesis is also assessed based on the size of the defect. Based on the size of the defect, the right defect shows no significant value. The size of the left defect shows a significant value between day 0 and day 30, and 90. The relative bone density of the right defect shows a significant value between day 7 and 30. In the left defect, the relative bone density shows the significant between 0 and 90. The relative density of the muscle does not show significant values either on the right or left defects.