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Patogenisitas Jamur Entomopatogen terhadap Stadia Telur dan Larva Hama Kubis Crocidolomia pavonana Fabricius Hasyim, Ahsol; Nuraida, -; Trizelia, -
Jurnal Hortikultura Vol 19, No 3 (2009): September 2009
Publisher : Indonesian Center for Horticultural Research and Development

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Abstract

Penelitian dilaksanakan di Laboratorium Entomologi dan Fitopatologi Universitas Andalas Padangdari bulan Mei sampai September 2006. Tujuan penelitian untuk mengetahui patogenisitas isolat-isolat jamurentomopatogen terhadap stadia telur dan larva hama kubis Crocidolomia pavonana. Penelitian ditata dalam rancanganacak lengkap pola faktorial dengan 2 faktor, yaitu 7 isolat ditambah 1 kontrol dan 5 stadia larva hama kubis. Hasilpenelitian menunjukkan bahwa efektivitas masing-masing isolat jamur entomopatogen berbeda nyata terhadapberbagai stadia telur dan larva hama ulat kubis. Hasil uji patogenisitas menunjukkan bahwa jamur Beauveria (isolatBAP5 dan BPP1), Nomuraea (isolat NAP3), Metarhizium (isolat MAP1), Paecilomyces (isolat PPP4), dan Fusarium(isolat FPP3) adalah jamur yang virulen terhadap telur dan larva C. pavonana. Jamur B. bassiana mempunyai potensiuntuk dikembangkan sebagai agens pengendali hama kubis pada masa mendatang.ABSTRACT. Hasyim, A., Nuraida, and Trizelia. 2009. Pathogenicity of Entomopathogenic Fungi Isolates toEggs and Larvae of Cabbage Head Caterpillar, Crocidolomia pavonana F (Lepidoptera: Pyralidae). This studywas carried out at Entomology and Phytopathology Laboratory Faculty of Agriculture, Andalas University fromMay to September 2006. The objectives of this study were to assess the pathogenicities of the entomopathogenicfungi isolates to eggs and larvae of C. pavonana. A completely randomized design with 2 factors i.e. 7 isolates ofentomophatogenic fungi with a control and 5 larvae stages of cabbage caterpillars were used. The results showedthat there were significant difference in infectivity of all isolates tested to eggs and larvae stages of the caterpillar.The isolates of Beauveria (isolate BAP5 and BPP1), Nomuraea (isolate NAP3), Metarhizium (isolate MAP1)w,Paecilomyces (isolate PPP4), and Fusarium (isolate FPP3) were more virulence to eggs and larva stages of C.pavonana. Beauveria bassiana was potential for controlling C. pavonana in near future
Virulence of Entomopathogenic Fungus Beauveria Bassiana Isolates to Crocidolomia Pavonana F (Lepidoptera: Crambidae) ., Trizelia; Nurdin, Firdos
AGRIVITA, Journal of Agricultural Science Vol 32, No 3 (2010)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The purpose of this research was to identify the virulent isolates of Beauveria bassiana (Bals.) Vuill. to larvae of Crocidolomia pavonana (F.) and also to investigate the effects of conidial concentrations on larval mortality of C. pavonana. This experiment was conducted at Laboratory of Biological Control, Plant pest and diseases Department, Faculty of Agriculture, Andalas University from April to October 2008. B. bassiana were collected from insects and soils then cultured using selective medium. Thirteen isolates and four different concentration conidia(106-109 conidia/ml) were tested in the experiment. Larvae directly inoculated with conidial suspensions of entomopathogenic fungus. The results showed at a concentration of 108 conidia/ml, isolate HhTK9 was the most virulent caused 82.50% mortality of 2nd instar larvae, with LT50 3.39 days. The mortality C. pavonana larvae was positively correlated with by fungal isolate and conidial concentration.Keywords:  Entomopathogenic, Beauveria bas-siana, virulence, isolates, Croci-dolomia pavonana
Pathogenicity of entomopathogenic fungus Metarhizium spp. against predators Menochilus sexmaculatus Fabricius (Coleoptera: Coccinellidae) TRIZELIA, TRIZELIA; BUSNIAH, MUNZIR; PERMADI, AGUNG
Asian Journal of Agriculture Vol 1 No 01 (2017)
Publisher : Society for Indonesian Biodiversity & Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g010101

Abstract

Trizelia, Busniah M, Agung Permadi A. 2017. Pathogenicity of entomopathogenic fungus Metarhizium spp. against predators Menochilus sexmaculatus Fabricius (Coleoptera: Coccinellidae). Asian J Agric 1: 1-5. Metarhizium spp is an entomopathogenic fungus which has wide host range. Metarhizium spp can not only infect insect pests, but can also infect beneficial insects such as predators. The pathogenicity of four isolates of Metarhizium spp. was investigated against Menochilus sexmaculatus predator. Isolates of Metarhizium spp were isolated from rhizosphere of cacao, cabbage, chili, and rubber crops. The experiment was conducted by treating the fourth instar larvae with an appropriate conidial suspension of 108 conidia mL-1. The results showed that all isolates of Metarhizium spp were pathogenic toward beetle predator M. sexmaculatus. Mortality of larvae within seven days after application of conidial suspension varied between 27.50 to 67.50% and there were statistically significant differences among the tested isolates. Metarhizium spp. had also a significant affect in reducing pupation and adult emergence of M. sexmaculatus to below 30% and 3%, respectively. These studies indicate that entomopathogenic fungus Metarhizium spp was pathogenic to beetle predators, M. sexmaculatus.
Keragaman Genetik berbagai Isolat Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) dan Virulensinya terhadap Crocidolomia pavonana Trizelia, Trizelia; Santoso, Teguh; Sosromarsono, Soemartono; Rauf, Aunu; Sudirman, Lisdar I
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.998 KB) | DOI: 10.31258/jnat.14.3.176-183

Abstract

The objectives of the research were to study the genetic variability of various Beauveria bassiana (Bals.) Vuill. isolates andvirulence to Crocidolomia pavonana. Thirteen isolates of B. bassiana were isolated from Nilaparvata lugens (Stål),Leptocorisa oratorius (F.), Thrips sp., Hypothenemus hampei (Ferr.), Crocidolomia pavonana (F.), Spodoptera litura (F.),Chrysodeixis chalcites (Esp.), and Riptortus linearis (L.) pests from different geographical locations in Indonesia. Fourteendifferent random primers were used to amplify DNA. Clustering of isolates were obtained using Unweight Pair GroupMethod Avarage (UPGMA). The results showed that only OPA08 primer generated more polymorphic DNA fragments.Result of clustering analysis showed that B. bassiana isolates forms two groups having genetic similarity equal to 55%.Clustering of isolates does not correlate to host type nor geographic origin. Mortality of second instar C. pavonana larvaewas dependent on the fungal isolates. At a concentration of 108 conidia/ml, isolate Bb-La2 had the highest virulence whichcaused 65% mortality of 2nd instar larvae. Isolates of B. bassiana is virulent against C. pavonana larvae present in the samegenotype group.
Profil GCMS Senyawa Kimia Ekstrak Metanol Isolat Cendawan Entomopatogen Beauveria Bassiana Dan Akar Cabai Sebagai Pemacu Pertumbuhan Cabai Saragih, Magdalena; Trizelia, Trizelia; Nurbailis, Nurbailis; Yusniwati, Yusniwati
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol 4, No 2 (2020): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.232 KB) | DOI: 10.31289/agr.v4i2.3840

Abstract

The aim of this study was to identify the chemical compound methanol extract of entomopathogenic fungus Beauveria bassiana from insect walang sangit and the chemical compound roots of red chili plants that were able to stimulate the growth of chilli plants after being applied with entomopathogenic fungus   B. bassiana through seed immersion inoculation using GCMS method. The chemical compound identified as a growth booster in   B. bassiana fungus isolates from the insect walang sangit is an Acetic acid Ethanoic acid Ethylic acid Glacial acetic acid CH3COOH, Hexadecanoic acid, methyl ester (CAS) Methylpalmitate Uniphat A60, n-Hexadecanoic acid Hexadecanoic acid n-Hexadecoic acid Palmitic acid, Dianhydromannitol and Ergosta-5,7,22-trien-3-ol, (3.beta., 22E) - (CAS) Ergosterol (CAS), while the chili root contains Acetic acid Ethanoic acid Ethylic acid Glacials acetic acid CH3COOH, Hexadecanoic acid, methyl ester (CAS) Methyl palmitate Uniphat A60, n -Hexadecanoic acid Hexadecanoic acid Palmitic acid, 8, 11- octadecadienoic acid, methyl ester (CAS) METHYL 8, 11-OCTADECADIENOATE, (23S)-ethylcholest-5-en-3.beta.-ol.s It can be concluded that some of the chemical compounds in methanol extracts identified in chili roots have similarities with chemical compounds that exist in   B. bassiana entomopathogenic fungus which are potential as stimulers of chili plant growth
Potensi Rizobakteri dalam Mengendalikan Hama Crocidolomia pavonana F. (Lepidoptera: Crambidae) Pada Tanaman Kubis. Indri Yanil Vajri; Trizelia Trizelia; Haliatur Rahma Haliatur Rahma
AGRIUM: Jurnal Ilmu Pertanian Vol 24, No 1 (2021)
Publisher : UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/agrium.v23i2.6911

Abstract

Crocidolomia pavonana merupakan salah satu hama penting yang membatasi produksi tanaman kubis. Alternatif pengendalian dengan penggunaan mikrorganisme yang berpotensi menekan perkembangan hama ini diantaranya dengan memanfaatkan rizobakteri. Tujuan penelitian adalah untuk mendapatkan isolat rizobakteri yang virulen terhadap larva C. pavonana. Penelitian dilaksanakan di Laboratorium Pengendalian Hayati, Fakultas Pertanian, Universitas Andalas, Padang menggunakan Rancangan Acak Lengkap (RAL) dengan 10 perlakuan dan 5 ulangan. Perlakuan terdiri dari beberapa isolat rizobakteri diantaranya 3 isolat Bacillus thuringiensis (KJKB2, KJKB3 dan BAKB), Bacillus subtilis (KJTSB), 3 isolat Serratia marcescens (AR1, AR2 dan RK10), Stenotrophomonas maltophilia (LMTSA) serta kontrol negatif (Aquadest steril) dan kontrol positif (insektisida Sipermetrin). Uji Virulensi dilakukan dengan metode perendaman pakan dalam suspensi yang mengandung rizobakteri dengan kerapatan populasi 10? sel/ml. Variabel yang diamati adalah mortalitas larva, persentase pembentukan pupa dan imago serta preferensi oviposisi. Data dianalisis dengan sidik ragam dan dilanjutkan dengan uji lanjut BNT pada taraf 5%. Hasil penelitian menunjukkan bahwa semua isolat rizobakteri dapat mematikan larva C. pavonana dan mampu menghambat perkembangan biologi serangga tersebut. Bacillus thuringiensis KJKB3 menunjukkan hasil yang lebih baik dengan nilai mortalitas paling tinggi (64%) dan nilai LT?? yang paling pendek (6.31 hari). Semua perlakuan tidak berpengaruh terhadap preferensi oviposisi, namun perlakuan isolat S. marcescens mampu menurunkan jumlah telur yang diletakkan pada tanaman perlakuan.
VIRULENSI BERBAGAI ISOLAT JAMUR ENTOMOPATOGEN METARHIZIUM SPP. TERHADAP HAMA PENGGEREK BUAH KAKAO CONOPOMORPHA CRAMERELLA SNELL. (LEPIDOPTERA: GRACILLARIIDAE) . Trizelia; . Nurbailis; Dina Ernawati
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.742 KB) | DOI: 10.23960/j.hptt.213151-158

Abstract

Virulence of various Metarhizium spp. (Deuteromycotina: Hyphomycetes) isolates on cocoa pod borrer pest, Conopomorpha cramerella Snell. (Lepidoptera: Gracillariidae). In Indonesia, Conopomorpha cramerella Snell. (Lepidoptera : Gracillariidae) is a serious pest of cocoa. Entomopathogens such as Metarhizium spp. (Deuteromycotina: Hyphomycetes) can be used to control this pest as an alternative to chemical insecticides. The purpose of this research was to study the virulence of isolates of Metarhizium spp. to pupae of C. cramerella. Nine isolates of Metarhizium spp each with 107 conidia/ml rate were used in the experiment. Pupal mortality was recorded daily. The results showed that isolate MetApCi from chili rhizosphere had the highest virulence causing 96.67% mortality of C. cramerella pupae and with an LT50 of 3.04 days.
KOMPATIBILITAS CENDAWAN ENTOMOPATOGEN BEAUVERIA BASSIANA (BALS) VUILL (DEUTEROMYCOTINA: HYPHOMYCETES) DENGAN MINYAK SERAI WANGI . Trizelia; Rusdi Rusli
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 1 (2012): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.962 KB) | DOI: 10.23960/j.hptt.11278-84

Abstract

Entomopathogenic fungi such as Beauveria bassiana are important natural control agents of many insects and can be potentially used as a bioinsecticide against several pests. Other potential source of bioinsecticide is certain plants such as fragrant lemongrass oil. The in vitro compatibility of the entomopathogenic fungus B. bassiana and fragrant lemongrass oil was evaluated. Fragrant lemongrass oil was tested in three different concentrations (0.1, 0.3 and 0.5%). Fragrant lemongrass oil was diluted in the steril SDAY medium at the different concentrations. Effects of these concentrations on conidia germination, colony growth and sporulation were compared. Fragrant lemongrass oil affected conidial germination, colony growth and sporulation of B. bassiana. Fragrant lemongrass oil was not compatible with the entomopathogenic fungus B. bassiana.
Virulence of Entomopathogenic Fungus Beauveria Bassiana Isolates to Crocidolomia Pavonana F (Lepidoptera: Crambidae) Trizelia .; Firdos Nurdin
AGRIVITA, Journal of Agricultural Science Vol 32, No 3 (2010)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v32i3.22

Abstract

The purpose of this research was to identify the virulent isolates of Beauveria bassiana (Bals.) Vuill. to larvae of Crocidolomia pavonana (F.) and also to investigate the effects of conidial concentrations on larval mortality of C. pavonana. This experiment was conducted at Laboratory of Biological Control, Plant pest and diseases Department, Faculty of Agriculture, Andalas University from April to October 2008. B. bassiana were collected from insects and soils then cultured using selective medium. Thirteen isolates and four different concentration conidia(106-109 conidia/ml) were tested in the experiment. Larvae directly inoculated with conidial suspensions of entomopathogenic fungus. The results showed at a concentration of 108 conidia/ml, isolate HhTK9 was the most virulent caused 82.50% mortality of 2nd instar larvae, with LT50 3.39 days. The mortality C. pavonana larvae was positively correlated with by fungal isolate and conidial concentration.Keywords: Entomopathogenic, Beauveria bas-siana, virulence, isolates, Croci-dolomia pavonana
PEMANFAATAN AGEN HAYATI UNTUK PENGELOLAAN OPT CABAI PADA KELOMPOK TANI SIMABUR SUKSES MAKMUR DI KECAMATAN PARIANGAN KABUPATEN TANAH DATAR Trizelia Trizelia; Nurbailis Nurbailis; Yulmira Yanti; Winarto Winarto; Haliatur Rahma; Martinius Martinius; Yenny Liswarni; Rusdi Rusli; Yunisman Yunisman; Darnetty Darnetty; Eri Sulyanti
Jurnal Hilirisasi IPTEKS Vol 2 No 3.b (2019)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (940.813 KB) | DOI: 10.25077/jhi.v2i3.b.331

Abstract

Cabai merah merupakan sumber pendapatan utama bagi petani di daerah Tanah Datar. Masalah utama yang selalu dihadapi oleh petani cabai di daerah ini adalah serangan hama dan penyakit yang dapat menyebabkan kehilangan hasil yang cukup tinggi. Hingga saat ini, pengendalian hama dan penyakit sayuran masih mengandalkan pestisida sintetik yang telah menimbulkan dampak negatif. Untuk itu, perlu dicari alternatif pengendalian yang dapat mengurangi dampak negatif pestisida tersebut, yaitu menggunakan agens hayati seperti cendawan Beauveria bassiana dan Trichoderma sp.Tujuan dari kegiatan pengabdian kepada masyarakat ini adalah untuk memperkenalkan dan memasyarakatkan pengendalian hama dan penyakit menggunakan agens hayati Beauveria bassiana dan Trichoderma sp. Metode kegiatan pengabdian adalah penyuluhan, pelatihan dan praktek lapangan. Minat petani untuk menggunakan agens hayati terutama Beauveria bassiana dan Trichoderma cukup tinggi dan telah memotivasi mereka untuk mengurangi penggunaan pestisida sintetis. Petani juga telah berhasil memperbanyak agens hayati dan membuat kompos limbah pertanian menggunakan Trichoderma.