Articles
<![CDATA[Relationship between Nucleus Swelling and Development Competence of Bovine Cloned Embryos Reconstructed by Enucleated Oocytes with Serum-starved or Serum-fed Fetal Somatic Cells ]]>
<![CDATA[Fahrudin, Mokhamad]]>;
<![CDATA[Karja, Ni Wayan Kurniani]]>;
<![CDATA[Suzuki, Tatsuyuki]]>
<![CDATA[ Indonesian Journal of Biotechnology Vol 10, No 2 (2005) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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<![CDATA[This study was conducted to examine the occurrence of nuclear remodeling (nucleus swelling) and its effectson the subsequent in vitro development of bovine embryos reconstructed by serum-starved and serum-fed somaticcells. Results from this study demonstrated that all of the reconstructed embryos that received serum-starved andserum-fed somatic cells exhibited condensed-nuclei. More than 90% of the transferred nuclei exhibited nuclearenvelope breakdown and premature chromatin condensation which clearly distinct from an intact nucleus. Therewas no significant difference on the degree of nucleus swelling in SS-NT embryos or SF-NT embryos, indicatingthat either serum-starved or confluent somatic cell lines could be reprogrammed by the recipient cytoplasmenvironments in similar pattern. Although the fusion rate was not significantly different among the groups, theproportion of SS-NT embryos which developed to the 2- to 4-cell stage (89.7%) and to the 8- to 16-cell stage (74.7%)was significantly higher than that of SF-NT embryos. Whereas, the proportion of reconstructed embryos thatdeveloped to the morula and blastocyst stages were not significantly different among the groups. Results of thesestudies demonstrate that reconstructed embryos, which received either serum-starved or serum-fed confluentsomatic cells, showed similar developmental competence to the blastocyst stage.Keywords: nuclear transplantation technique-somatic cells-nucleus swelling]]>
<![CDATA[Developmental Competence of Early Stage Porcine Embryos Cultured in Medium with Different Energy Substrate in vitro ]]>
<![CDATA[Karja, Ni Wayan Kurniani]]>;
<![CDATA[Kikuchi, Kazuhiro]]>;
<![CDATA[Fahrudin, Mokhamad]]>
<![CDATA[ Indonesian Journal of Biotechnology Vol 11, No 1 (2006) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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<![CDATA[To elucidate the effect of energy requirement during the early embryonic development on their developmentalability to develop to blastocyst stage, in vitro fertilized (IVF) porcine one-cell embryos were cultured in modifiedNorth Carolina State University (NCSU)-37 supplemented with different energy substrate. Result indicated that thecleavage rate of embryos in Pyr-Lac and Gluc-Pyr-Lact groups was significantly higher than in those in Gluc groupand Gluc-Rib group (P < 0.05). At Day 6 of culture, the highest proportion of embryos develop to the blastocyst stagewas obtained in the presence of pyruvate-lactate only. In the medium with glucose, the addition of pyruvate-lactateor ribose slightly increased the proportion of embryos develop to the blastocyst stage, however the value were notsignificantly different form those obtained in the presence of glucose only. The mean cell number in blastocystsderived from Pyr-Lac and Gluc-Pyr-Lact groups were significantly higher than those in the Gluc group (P < 0.05).These results indicated that the presence of glucose only, as energy substrate, during the first 2 days of in vitro culture(IVC) caused a decrease in development of in vitro produced (IVP) porcine embryos to the blastocyst stage and meancell number in blastocysts .Keywords: porcine embryos-energy substrate-in vitro culture]]>
<![CDATA[Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium ]]>
<![CDATA[Budiariati, Vista]]>;
<![CDATA[Budiono, Dwi]]>;
<![CDATA[Fahrudin, Mokhamad]]>;
<![CDATA[Juliandi, Berry]]>;
<![CDATA[Rinendyaputri, Ratih]]>;
<![CDATA[Boediono, Arief]]>
<![CDATA[ HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020 ]]>
Publisher : <![CDATA[Bogor Agricultural University, Indonesia ]]>
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DOI: 10.4308/hjb.27.2.89
<![CDATA[Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.]]>
<![CDATA[Analisis Proteomik Cairan Sinovial Sendi Domba: Efektivitas Metode dan Profil Protein Fungsional ]]>
<![CDATA[Mohamad, Kusdiantoro]]>;
<![CDATA[Rahmaniyah, Wiwit Ridhani]]>;
<![CDATA[Adnyane, I Ketut Mudite]]>;
<![CDATA[Fahrudin, Mokhamad]]>;
<![CDATA[Boediono, Arief]]>
<![CDATA[ Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020 ]]>
Publisher : <![CDATA[IPB University ]]>
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DOI: 10.29244/avi.8.2.52-64
<![CDATA[Penelitian ini bertujuan untuk mengevaluasi metode deplesi dan digesi protein, serta menganalisis proteom cairan sinovial (SF) sendi pada domba sehat menggunakan kromatografi cair–tandem spektrometri massa. Cairan SF dikoleksi dari enam ekor domba garut betina, umur ±4 tahun, berat 35-40 kg, dan sehat secara klinis. Deplesi protein berkelimpahan tinggi dilakukan dengan metode spin column menggunakan TOP 12 dan metode proteospin, sementara digesi protein dengan tiga metode, yaitu digesi in solution, in gel, dan in-solution + filter-aided sample preparation (FASP). Metode yang terbaik kemudian dilanjutkan dengan fraksinasi peptida menggunakan ultra performance liquid chromatography. Metode deplesi proteospin dengan digesi protein in-solution + FASP merupakan metode terbaik berdasarkan nilai coverage dan sequest HT. Hasil analisis proteomik terkarakterisasi 52 protein pada database spesies domba. Anotasi gen ontologi menggunakan DAVID analysis menunjukkan bahwa protein-protein SF tersebut merupakan komponen sel terutama sebagai eksosom ekstraseluler, fungsi molekuler sebagai aktivitas inhibitor endopeptidase tipe-serin dan pengikat ion kalsium; serta proses biologis sebagai angiogenesis dan koagulasi darah atau pembentukan fibrin. Analisis KEGG pathway menunjukkan protein SF berperan utama pada jalur kaskade koagulasi dan komplemen.]]>
<![CDATA[Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In Vitro ]]>
<![CDATA[NI WAYAN KURNIANI KARJA]]>;
<![CDATA[MOKHAMAD FAHRUDIN]]>;
<![CDATA[KAZUHIRO KIKUCHI]]>
<![CDATA[ HAYATI Journal of Biosciences Vol. 16 No. 1 (2009): March 2009 ]]>
Publisher : <![CDATA[Bogor Agricultural University, Indonesia ]]>
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DOI: 10.4308/hjb.16.1.25
<![CDATA[In porcine preimplantation embryos, glucose-utilizing pathways are reported active throughout their development. The aim of this study was to test the involvement of glycolysis activity in early stage of porcine embryo development by the addition of iodoacetate, a glycolytic inhibitor, to culture medium. In vitro matured and fertilized oocytes were cultured for the first 2 days in iodoacetate at concentrations of 0, 1, or 2.5 µM. The proportion of viable embryos on day 4 decreased when Ia was added at concentration of 2.5 µM (P < 0.05). The development of cleaved embryos to the blastocyst stage on day 6 was significantly reduced by 1 µM iodoacetate and none of cleaved embryos in Ia-2.5 group develop to the blasyocyst stage (P < 0.05). More than 60% of embryos in Ia-2.5 group were arrested at the two-four-cell stage, and then arrested at the morula stage. These results indicate that glucose plays important roles in supporting the development of early stage of porcine embryos and that the inhibition of glucose metabolism may disrupt the management of energy production, leaving the embryos incompetence to develop. Key words: iodoacetate, glucose, early stage embryos, in vitro culture]]>
<![CDATA[Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium ]]>
<![CDATA[Vista Budiariati]]>;
<![CDATA[Dwi Budiono]]>;
<![CDATA[Mokhamad Fahrudin]]>;
<![CDATA[Berry Juliandi]]>;
<![CDATA[Ratih Rinendyaputri]]>;
<![CDATA[Arief Boediono]]>
<![CDATA[ HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020 ]]>
Publisher : <![CDATA[Bogor Agricultural University, Indonesia ]]>
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DOI: 10.4308/hjb.27.2.89
<![CDATA[Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.]]>
<![CDATA[Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) ]]>
<![CDATA[Noer Muhammad Dliyaul Haq]]>;
<![CDATA[Diah Pristihadi]]>;
<![CDATA[Vista Budiariati]]>;
<![CDATA[Dwi Budiono]]>;
<![CDATA[Al Mukhlas Fikri]]>;
<![CDATA[Mokhamad Fahrudin]]>;
<![CDATA[Cece Sumantri]]>;
<![CDATA[Arief Boediono]]>
<![CDATA[ HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021 ]]>
Publisher : <![CDATA[Bogor Agricultural University, Indonesia ]]>
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DOI: 10.4308/hjb.28.3.185
<![CDATA[Interspecies embryo transfer is a seldom-used method to increase the successful conservation of endangered species. The study aimed to determine the potential development of interspecies reconstructed embryos. The present study used two animal models, Campbell hamsters (Phodopus campbelli) and mice (Mus musculus). The isolated inner cell mass (ICM) of hamster embryos were injected into the mice embryos. The embryos were transferred to the pseudopregnant mice using non-surgery embryos transfer methods. The fetuses were collected at day 13.5 of gestation for morphometric measurement and cytochrome b (Cyt b) analysis which used to determine the species of obtained fetuses. The results showed that the viability, pregnancy rate, and embryonic implantation ability of the interpecies reconstructed embryos did not differ significantly (p>0.05) compared to non-reconstructed embryos. Morphometric measurement showed that the crown-rump (CR) and the weight of fetuses in the reconstructed group were significantly higher than non-reconstructed group (p<0.05). According to Cytb analysis, the species of obtained fetuses were mice, while the population of hamster cells were found only in the blighted ovum (resorption). Therefore, it can be concluded that interspecies reconstructed embryos are able to implant. However, the population of mice cells are only found to develop.]]>
<![CDATA[The safety evaluation of ethanol extract of torbangun leaves (Coleus amboinicus Lour.) on mouse fetal development ]]>
<![CDATA[Elma Alfiah]]>;
<![CDATA[Muhammad Rizal Martua Damanik]]>;
<![CDATA[Katrin Roosita]]>;
<![CDATA[Mokhamad Fahrudin]]>
<![CDATA[ Jurnal Gizi dan Pangan Vol. 13 No. 1 (2018) ]]>
Publisher : <![CDATA[Food and Nutrition Society of Indonesia ]]>
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DOI: 10.25182/jgp.2018.13.1.27-32
<![CDATA[The purpose of this study was to evaluate the safety of ethanol extract from torbangun leaves on mouse fetal development. This research used 24 female pregnant mouse. The leaves extract was administered orally at dose level of 0; 0.56; 1.68 and 3.36 g/kg body weight. The extract was given from the first day until the eighteenth day of pregnancy period. The ethanol extract of Torbangun leaves contains flavonoids, saponins, tannins, and steroids. The result showed that consumption of 3.36 g/kg torbangun leaves extract had led to significant differences in the decrease of maternal weight change, number of live fetuses, fetal weight, and fetal length. Consumption of Torbangun leaves extract during pregnancy should be avoided due to its potency to interfere the fetal development.  ]]>
<![CDATA[Calcium Bioavailability and Serum Calcium Level in Pregnant Rats After Administration of Milk-Based Drinks Containing Lactic Acid Bacteria ]]>
<![CDATA[Yuni Nurwati]]>;
<![CDATA[Hardinsyah Hardinsyah]]>;
<![CDATA[Sri Anna Marliyati]]>;
<![CDATA[Mokhamad Fahrudin]]>
<![CDATA[ Jurnal Gizi dan Pangan Vol. 16 No. 3 (2021) ]]>
Publisher : <![CDATA[Food and Nutrition Society of Indonesia ]]>
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DOI: 10.25182/jgp.2021.16.3.149-158
<![CDATA[This study aims to evaluate calcium bioavailability through serum calcium level in pregnant rats treated with two Milk-Based Drinks (MBD) containing Lactic Acid Bacteria (LAB), i.e Lactobacillus Casei Shirota Strain (LcS) drink, and Four Strains Bacteria (FS) (Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus delbrueckii subp. bulgaricus, Streptococcus thermophiles). It was a completely randomized experimental study using 24 Sprague Dawley rats. Rats were divided into one negative control group with a normal nutritional status (A0) and three undernourished groups. The undernourished groups were divided into one positive control group (A1), one group receiving MBD containing LcS group (A2), and another group receiving MBD containing FS (A3). All rats received high protein diets during pregnancy. The intervention was started in early pregnancy (D0) until 19 days of pregnancy (D19). Blood samples were collected at the D0 and D19. No significant differences in food intake were found among the rats in all groups. Administration of MBD containing LAB in A2 and A3 showed significant (p<0.05) increment of calcium bioavailability (30.79±6.88%; 20.44±9.04%). Both MBDs treatment containing LAB showed no significant difference in serum calcium bioavailability (p>0.05). The results suggest that MBDs containing LAB are useful in enhancing calcium bioavailability.]]>
<![CDATA[Allotransplantasi Testis Mencit Muda sebagai Upaya Preservasi Gonad In Vivo ]]>
<![CDATA[Wahono Esthi Prasetyaningtyas]]>;
<![CDATA[Kusdiantoro Mohamad]]>;
<![CDATA[Mokhamad Fahrudin]]>;
<![CDATA[Ita Djuwita]]>;
<![CDATA[Srihadi Agungpriyono]]>
<![CDATA[ Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 1 (2007): Jurnal Ilmu Pertanian Indonesia ]]>
Publisher : <![CDATA[Institut Pertanian Bogor ]]>
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<![CDATA[Cancer disease are not detected only at adult but also at young age. One therapy for cancer diseases is chemotherapy and radiation, that give a side effect of infertility in the gonad, therefore, it is necessary to preserve the gonad. Sperm collection from adult is easy but not from the young patients.Keyword: transpalantation, testis, mencit muda, spermatogenesis]]>
