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All Journal HAYATI Journal of Biosciences International Journal of Integrated Health Sciences Majalah Kedokteran Bandung JURNAL WIDYA MEDIKA Jurnal Farmasi Klinik Indonesia Majalah Kedokteran Gigi Indonesia Molecular and Cellular Biomedical Sciences (MCBS) Majalah Patologi Indonesia Jurnal Ilmu Bedah Indonesia Makara Journal of Health Research
Ahmad Faried
Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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CRANIAL CHARACTERISTICS, MAXILLOFACIAL, AND SKULL BASE STRUCTURE OF NON-HUMAN PRIMATE (ADULT MACACA FASCICULARIS): A PRELIMINARY STUDY FOR CRANIAL CRANIOTOMY MODEL Bolly, Hendrikus Masang Ban; Indah, Astrina Rosaria; Faried, Ahmad; Noverina, Rachmawati; Arifin, Muhammad Zafrullah; Wirakusumah, Firman Fuad
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n2.1650

Abstract

The use of non-human primate (NHP) animal models, which anatomical and physiological similarities to human, is important for the sake of learning the anatomical properties. This study aimed to characterize the cranial, maxillofacial, and skull base structures of non-human primates as a potential model suitable for a cranial craniotomy model. Adult Macaca fascicularis (MF) skulls classified asspecificpathogen-free for TB, SIV, SV40, Polio, Foamy virus and Herpes B virus from PT Bio Farma (Persero) Animal Lab. Library were used to represent the anatomical model.The open access database from Mammalian Crania Photographic Archive 2nd Edition (MCPA2) was used for cranial characterization analysis. This study was performed at the Department of Neurosurgery, Dr. Hasan Sadikin General Hospital and the Animal Laboratory of PT. Biofarma (Persero) from November 2018 to January 2019. The skull base structures were assessed for its analogies with its human counterpart. Comparison by t-student analysis between male and female skulls shows the mean male cranial length (CL) is greater than in female (116.68 vs 102.50 mm), with p=0.000; the mean male bizygomatic width (BZB) is greater than in female (79.30 vs 69.70 mm) with p=0.001; the mean male posterior cranial breadth (CBN) is greater than in female (63.40 vs 58.79 mm) with p=0.019; and the mean male cranial base length (CBL) is greater than in female (63.32 vs 57.55 mm), with p=0.001. The skull of MF is suitable for Neurosurgery and Neuroscience study since the MF cranial characterization and structure are similar to that of human. Its structure is ideal for performing craniotomy since it has several characteristics such as cranial vault, maxillofacial structure with huge temporal muscle, and skull base structure.  Karakteristik Kranium, Maksilofasial, dan Struktur Dasar Tengkorak Non-human Primate Macaca fascicularis dewasa: Studi Pendahuluan untuk Model Craiotomi Kranium Penggunaan hewan model berasal dari primata bukan-manusia (NHP), dimana secara anatomis dan fisiologis mirip dengan manusia, sangat penting untuk dapat mempelajari struktur anatominya. Penelitian ini bertujuan untuk karakterisasi struktur kranium, maksilofasial dan dasar tengkorak primata (Non-human Primate) yang berpotensi sebagai model penelitian kraniotomi. Tengkorak dewasa Macaca fascicularis (MF) dari  Laboratorium Hewan PT Biofarma (Persero), bebas infeksi TBC, SIV, SV40, Polio, Foamy Virus,  dan Herpes B digunakan sebagai model anatomi. Basis data terbuka Mammalian Cranial Photographic Archive 2nd Edition (MCPA2) digunakan untuk analisis kranium. Penelitian dilakukan di Departmen Bedah Saraf RSHS dan Laboratorium Hewan  PT. Biofarma  pada November 2018?Januari 2019. Struktur tengkorak MF dipelajari analogi seperti pada struktur tengkorak manusia. Analisis uji-t tengkorak jantan dan betina menunjukan rerata panjang kranial (Cranial length, CL) jantan lebih panjang dibanding betina (116.68 vs 102.50 mm, p=0.000); rerata bizygomatic width (BZB) jantan lebih panjang dibanding betina (79.30 vs 69.70 mm, p=0.001); rerata posterior cranial breadth (CBN) jantan lebih panjang dibanding betina (63.40 vs 58.79 mm, p=0.019);dan rerata cranial base length (CBL) jantan lebih panjang dibanding betina (63.32 vs57.55 mm, p=0.001). Tengkorak MF sesuai untuk berbagai penelitian bidang bedah saraf dan neurosains. Karakterisasi kranial dan strukturnya sama dengan yang dimiliki manusia. Struktur kranium MF merupakan model ideal untuk kraniotomi berdasar karakteristik tulang tengkorak, otot temporal besar pada maksilofasial, dan struktur dasar tengkorak.
Incidence of Cavum Septum Pellucidum and Cavum Vergae in Dr. Hasan Sadikin General Hospital, Bandung, Indonesia Faried, Ahmad; Soeprajogo, Adolf Setiabudi; Melia, Regina; Arifin, Muhammad Zafrullah
International Journal of Integrated Health Sciences Vol 5, No 1 (2017)
Publisher : International Journal of Integrated Health Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.788 KB)

Abstract

Objective: To describe the cavum septum pellucidum (CSP) and cavum vergae (CV) findings. Methods: Thirteen samples were recruited in the study. The study was conducted at the Department of Neurosurgery, Dr. Hasan Sadikin Hospital, Bandung, Indonesia in the period of 2015–2016. Thirteen samples were recruited for the study. Cavum septum pellucidum and CV spaces are relatively rare. During neurology and neurosurgery examination of patients with head computed tomography (CT) scan and magnetic resonance imaging (MRI), CSP and CV were discovered accidentally. The incidence of these unique spaces reported from many countries, but have never been reported from Indonesia.Results: This is the first-time reported incidental finding of CSP and CV from Indonesia, 13 cases in one-year period. Since the presence of CSP and CV is usually asymptomatic, clinician seem don’t really care to put it in their report.Conclusions: The presence of CSP and CV as a common incidental finding can be more revealed in our country in years to come and hopefully can be more studied in term of anatomical landmark on neurosurgical field.Keywords: Cavum septum pellucidum, cavum vergae, incidental finding DOI: 10.15850/ijihs.v5n1.964
Akt–the Mammalian Target of Rapamycin (mTOR) Pathway Inhibition Increases Cervical Cancer Cell Chemosensitivity to Active Form of Irinotecan (SN-38) Septiani, Leri; Hidayat, Yudi Mulyana; Effendi, Yusuf Sulaeman; Djuwantono, Tono; Luftimas, Dimas Erlangga; Faried, Ahmad
International Journal of Integrated Health Sciences Vol 1, No 1 (2013)
Publisher : International Journal of Integrated Health Sciences

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Abstract

Objective: To investigate the molecular pathway of the cytotoxic effect of SN-38 in human cervical cancer cell lines.Methods: Two human cervical cancer cell lines were treated with various concentrations of irinotecan for 24–72 hours and the sensitivity was analysed using the MTT assay. Apoptosis was further observed through microscopic examinations. The protein expression was determined using Western blot analysis.  Results: CaSki cells demonstrated the highest sensitivity to SN-38, whereas HeLa cells showed the lowest. In cervical cancer cells, SN-38 induced apoptosis through an intrinsic- and extrinsic-pathways. In addition, we showed that SN-38 downregulated the phosphorylation of Akt-mTOR pathways in CaSki cells, but not in HeLa cells. Interestingly, in HeLa cells, which were more suggestive of a resistant phenotype, pre-treatment with LY294002 and rapamycin inhibited activation of Akt-mTOR signaling and significantly enhanced the sensitivity of HeLa cells to SN-38. Conclusions: Irinotecan exerts its anti-neoplastic effects on cervical cancer cells by inducing apoptosis through caspase-cascade. Inhibition of Akt-mTOR, LY294002 and rapamycin, which is targeted to Akt-mTOR pathways, may sensitize irinotecan-resistant cervical cancer cells.Keywords: Akt-mTOR pathways anti-neoplastic drugs, cervix cancer cells, LY294002, rapamycin DOI: 10.15850/ijihs.v1n1.103
COMPARISON OF HISTOLOGICAL CHARACTERISTICS OF DRIED AND FRESH AMNION MEMBRANES AND DURA MATER IN NON-HUMAN PRIMATE (MACACA FASCICULARIS) Indah, Astrina Rosaria; Bolly, Hendrikus Masang B.; Faried, Ahmad; Arifin, Muhammad Zafrullah; Achadiyani, Achadiyani; Wirakusumah, Firman Fuad
Majalah Kedokteran Bandung Vol 51, No 1 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.785 KB) | DOI: 10.15395/mkb.v51n1.1651

Abstract

This study aimed to characterize the histological properties of dry-lyophilized amniotic membrane, fresh amniotic membrane (AM), and duramater membrane in search for a biologically-derived material suitable for meninges surface reconstruction. This descriptive study was conducted at the Unit-3 Laboratory of Animal Test of PT. Bio Farma (Persero), Bandung and Cell Biology Laboratory of the Faculty of Medicine, Universitas Padjadjaran Bandung. This study was conducted from 2018-2019. Fresh Macacafascicularis placenta from healthy donors,classified as specific pathogen-free for TB, SIV, SV40, Polio type 1,2,3, Foamy virus and Herpes B virus, were obtained from selected caesarean sections.The harvested dried and fresh AM and duramater membrane were stained with hematoxylin-eosin and prepared for characterization. Histological examination of dry-lyophilized and fresh AM showed similar results. Histologically, AM is represented by a single layer of metabolically-active cuboidal to columnar epithelium with microvilli firmly attached to a basement membrane and an avascular and relatively sparsely populated stroma. Meninges layers consists of 3 layers: duramater, arachnoid membrane, and piamater. Most of these cells have the same characteristics as fibroblasts, including long organelles and nuclei with various levels of fibril formation. The histological study of amniotic membrane and duramater membrane shows comparable results. The AM is a biologically-derived material suitable for meninges surface reconstruction since its histological structure is somewhat similar to that of the duramater. Its structure is ideal for replacing duramater since it has several characteristics, such as having hygroscopic properties, good biocompatibility, relatively easy to apply, and inexpensive. Key words: Dried and fresh amnion membranes, duramater membranes, non-human primate Karakteristik Histologis Membran Amnion Jenis Kering dan Segar dengan Membran Duramater pada Primata Non-Human Macaca fascicularisPenelitian ini bertujuan mengetahui karakteristik histologis membran amnion kering yang diliofilisasi, membran amnion segar, dan duramater, dalam rangka mencari bahan biologis yang cocok untuk rekonstruksi permukaan meninges. Penelitian deskriptif dilakukan di Laboratorium Hewan Uji PT. Bio Farma dan Laboratorium Biologi Sel FK Universitas Padjadjaran periode 2018-2019. Plasenta Primata non-human Macaca fascicularis segar dari donor sehat, yang bebas dari pathogen spesifik TB, SIV, SV40, Polio tipe 1, 2, 3, virus Foamy dan virus Herpes B, diperoleh dari seksio sesarea. Kemudian, dilakukan pewarnaan dengan hematoxylin-eosin untuk membran amnion kering dan segar, serta membran duramater untuk mengetahui karakterisasi histologisnya. Pemeriksaan histologis membran amnion kering-yang aktif bermetabolisme hingga kolumnar dengan mikrovili; melekat kuat pada membran basal dan stroma yang avaskular dan relatif jarang. Lapisan Meninges terdiri dari 3 lapisan: duramater, arachnoid dan piamater. Sebagian besar sel-sel ini memiliki karakteristik yang sama dengan fibroblas. Studi histologis membran amnion dan membran duramater memiliki struktur yang relatif serupa. Membran amnion adalah material yang secara biologis cocok untuk rekonstruksi permukaan meningen, karena struktur histologinya agak mirip dengan duramater. Oleh karena itu secara struktur, membran amnion ideal untuk menggantikan duramater karena memiliki beberapa karakteristik seperti sifat higroskopis, biokompatibilitas baik, mudah diterapkan, dan murah.Kata kunci: Membran amnion segar dan kering, membran duramater, primate non-human
MODIFIKASI METODE ISOLASI SEL ENDOTEL PEMBULUH DARAH OTAK (EPDO) TIKUS: TEKNIK DASAR KULTUR SEL PRIMER DI BIDANG NEUROSAINS Faried, Ahmad; Zafrullah Arifin, Muhammad; Sutiono, Agung Budi; Halim, Danny; Djuwantono, Tono; Achmad, Tri Hanggono
Majalah Kedokteran Bandung Vol 42, No 4
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1444.737 KB)

Abstract

Metode konvensional isolasi sel endotel pembuluh darah otak (EPDO) masih tergolong sulit, sehingga upaya mendapatkan populasi murni sel ini adalah tantangan. Pada penelitian ini dilakukan isolasi endotel dari tikus Wistar dan mencit C57/Bl6, berdasarkan protokol the care and use of laboratory animals, Universitas Gunma, Jepang. Modifikasi metode isolasi adalah menggunakan gradasi bovine serum albumin (BSA), bukan Dextran-70 yang umumnya dipakai, untuk memisahkan sel EPDO yang bersatu menjadi sel EPDO tunggal. Penelitian ini dilakukan di laboratorium sel kultur, Universitas Padjadjaran bekerjasama dengan Universitas Gunma, Jepang, Januari 2008?Juni 2009. Uji hasil isolasi dan karakteristik sel EPDO dilakukan dengan teknik imunofloresen. Ekspresi tight junction ZO-1, menunjukkan sel EPDO membentuk selapis sel utuh, rapat, tidak bertumpuk dan kompak, sesuai dengan karakteristik dinding EPDO. Fenotip sel EPDO dikonfirmasi dengan acethylated LDL, faktor von Willebrand dan CD31. Penghancuran kapiler dengan collagenase/dispase masih menghasilkan populasi sel yang terkontaminasi perisit. Kontaminasi dimurnikan dengan menggunakan puromycin, tingkat pemurnian sel EPDO mencapai 98,3%. Simpulan, teknik modifikasi berhasil mengisolasi sel EPDO tikus dan mencit, tanpa melakukan intervensi genetik. Puromycin dapat digunakan untuk memurnikan sel EPDO. [MKB. 2010;42(4):161?8].Kata kunci: Metode modifikasi isolasi sel EPDO, pembuluh sawar otak, teknik pemurnian Isolation Modified-Method of Mouse-Brain Microvessel Endothelial Cells: Primary Cell Culture Technique in NeuroscienceIsolation method to obtain pure BMVECs is hard to be done consistently and remains a challenge. In this study, we isolated BMVECs from Wistar rat and C57/Bl6 mouse from Japan SLC. All procedures performed according to guidelines for the care and use of laboratory animals of Gunma University, Japan. The modification of isolation method was using bovine serum albumin (BSA) gradation, not Dextran-70 in which generally used, to separate clusters of BMVECs into single cell. This study was done at Universitas Padjadjaran, in colaboration with Gunma University, Japan, January 2008?June 2009. Further,characteristic and purification results were proven by imunofluorescene staining. The results showed that staining of tight junction, ZO-1, formed a monolayer, tightly packed, non-overlapping and contact-inhibited BMVECs, as expected for a vessel wall endothelial. ECs phenotype confirmed by acethylated LDL, von Willebrand and CD31. The digestion of capillaries generated contaminating pericytes. Contamination was purified using puromycin and the results considered satisfactory (98.3%). In conclusion, our modification procedure allows the isolation of primary rat and mouse BMVECs, which form an endothelial-like monolayer in few days. Puromycin can be used for purification of primary rat and mouse BMVECs. [MKB. 2010;42(4):161?8].Key words: Blood brain barrier, isolation modified-method of mouse-BMVECs, purification methods DOI: http://dx.doi.org/10.15395/mkb.v42n4.30
Analisis Imunoekspresi Programmed Death Ligand 1 (PD-L1) dan Indeks Proliferasi Ki-67 pada Tumor Astrositik Difus Billy Parulian Lubis; Hasrayanti Agustina; Hermin Aminah Usman; Bethy Surjawathy Hernowo; Sri Suryanti; Ahmad Faried; Hendrikus Bolly
Majalah Patologi Indonesia Vol 29 No 2 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.15 KB) | DOI: 10.55816/mpi.v29i2.418

Abstract

BackgroundDiffuse astrocytic tumours (DAT) is a diffuse infiltrative astrocytoma tumor accompanied by molecular parameters in the form or absence ofIsocitrate Dehydrogenase (IDH) gene mutations. The DAT category without the examination of the IDH gene mutation is classified as NotOtherwise Specified (NOS). Ki-67 is a marker for DAT proliferation so that it can assess the degree of DAT. Diffuse astrocytic tumoursbehavior is influenced by proliferative ability and the presence of a tumor microenvironment. The tumor microenvironment PD-L1 factorinfluences DAT behavior and allows the development of target therapy in DAT cases. This study aimed to analyze the relationship betweenPD-L1 and Ki-67 imunoexpression in the histopathological degree of DAT.MethodsCross-sectional study of 30 paraffin blocks of DAT NOS cases from 2014-2018 in the Department of Anatomical Pathology RSUP dr. HasanSadikin, Bandung. The sample consisted of 15 cases of grade II, 7 cases of grade III and 8 cases of grade IV, carried outimmunohistochemical staining with GFAP, PD-L1 and Ki-67. Assessment of PD-L1 immunoexpression was carried out by the histoscoremethod. The Ki-67 assessment was assessed based on its distribution, which was <10% and ≥10%. PD-L1 and Ki-67 immunoexpressionwith histopathological degrees in DAT were statistically analyzed by Kolmogorof Smirnov test, however association betweenimmunoexpression of PD-L1 and Ki-67 were analyzed by chi-square test.ResultsThere was a significant relationship between PD-L1 immunoexpression and histopathological degree in DAT (p=0.005). There was asignificant relationship between Ki-67 immunoexpression and DAT histopathology degree and with PD-L1 (p=0.001 and p=0.010).ConclusionPD-L1 immunoexpression and proliferation rates affect the degree of DAT. Proliferation rate of DAT is influenced by PD-L1 so that in thisstudy, PD-L1 and Ki-67 can be used as a marker of prognosis predictors.
Evaluation Of Traumatic Brain Injuries Due To Alcohol Intoxication: Study In Central Hospital In West Java Hendrikus Masang Ban Bolly; Agung Budi Sutiono; Ahmad Faried; Trajanus Laurens Yembise; Muhammad Zafrullah Arifin; Benny Atmadja Wirjomartani
JURNAL WIDYA MEDIKA Vol 6, No 1 (2020): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (874.14 KB) | DOI: 10.33508/jwm.v6i1.2501

Abstract

Background: Alcohol intoxication is one of the predisposing factors for the incidence of traumatic brain injury (TBI). At present, there are no hospital-based specific data that provide TBI prevalence rates due to alcohol intoxication, especially in West Java. Objective: This study aims to make descriptive observations about the incidence of TBI due to alcohol intoxication in one of the central referral hospitals in West Java. Method: A retrospective cross-sectional study was carried out involving 10,662 TBI patients recorded in the emergency department database and the Department of Neurosurgery daily case database in the period 2012 to 2018. All data relating to the two items were analyzed. Alcohol intoxication is known from a history of autoanamnesis, alloanamnesis, and the subjective identification of the examiner of alcohol odor on the breath in TBI patients who enter the emergency room. Statistical cross-tabulations were performed to present clinical and demographic phenomena in TBI cases due to alcohol intoxication. Results: The analysis showed that the prevalence of alcohol intoxication in TBI cases was 4.77% (N = 507), all of which were due to motor vehicle accidents. Mean age of 26.44 ± 9.32 years (median 24 and range 12-64). The predominant age in TBI cases due to alcohol intoxication was in the age range of 20-30 years (44.2%), and male sex 92.7%. Most cases occurred in May (12%) and on Saturdays. Based on the consciousness assessment, initial Glasgow Coma Scale in emergency department were 14 (35.9%) and classified as mild TBI (69.2%). Subarachnoid hemorrhage and cerebral contusion are predominantly lesions found on CT scans of patients (2.2%), anterior skull base fractures of 8.1%, and linear fractures (5.1%) are the dominant extracranial lesions. Soft tissue lacerations are found in 16% of all cases. Craniotomy surgery was performed in 3.6% of patients for epidural, intracerebral, and subdural hemorrhage lesion evacuation as well as correction of depressed skull fractures. About 45% were treated for a minimum of three days to observe changes in their consciousness status before discharge. Conclusion: Alcohol consumption before the head injury in motor vehicle accidents has a relationship with various complications. Alcohol intoxication is a complex issue, and more research needs to be done to create comprehensive prevention programs and regulations.
Soybean is a good source of protein. It has two major fractions, b-conglycinin (7S) and glycinin (11S). b-conglycinin’s function was known to suppress food intake, and this effect may be due to stimulating endogenous cholecystokinin (CCK) release. The aims of this study were to determine the highest content of total b-conglycinin and b-conglycinin sub unit-b level obtained from two varieties of soybean i.e. Wilis and Detam 1 varieties using different preparation and extraction methods. These two MEILINAH HIDAYAT; MUCHTAN SUJATNO; NUGRAHA SUTADIPURA; . SETIAWAN; AHMAD FARIED
HAYATI Journal of Biosciences Vol. 18 No. 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.1.37

Abstract

Soybean is a good source of protein. It has two major fractions, b-conglycinin (7S) and glycinin (11S). b-conglycinin’s function was known to suppress food intake, and this effect may be due to stimulating endogenous cholecystokinin (CCK) release. The aims of this study were to determine the highest content of total b-conglycinin and b-conglycinin sub unit-b level obtained from two varieties of soybean i.e. Wilis and Detam 1 varieties using different preparation and extraction methods. These two soybean varieties were prepared into tempeh. Then the seed and tempeh were extracted using Deak and Panthee methods. There were six extracts analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) and coomassie brilliant blue (CBB) staining. The result was shown that Detam variety and raw seed contained the highest total b-conglycinin level. And Panthee method was the best method for extraction of total b-conglycinin, while Deak method was the best method for extraction of b-conglycinin subunit-b.
Apoptotic Potential of Secretome from Interleukin-Induced Natural Killer Cells toward Breast Cancer Cell Line by Transwell Assay Wahyu Widowati; Diana Krisanti Jasaputra; Teresa Liliana Wargasetia; The Fransiska Eltania; Alya Mardhotillah Azizah; Mawar Subangkit; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting; Ermi Girsang; Ahmad Faried
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.186

Abstract

Breast cancer (BC) is the number one cause of deaths from cancer in women. Metastasis in BC is caused by immunosurveillance deficiency, including impairment of Natural Killer (NK) cell maturation, low NK activity, and decreasing cytotoxicity. This study was performed to improve activating receptors and cytotoxicity of NK cells using interleukin 15 (IL15) against BC cells. Human recombinant IL15 was used to induce NK cells. To evaluate the potential of IL15 in inducing NK cells, we measured the activating and inhibiting receptors (NKG2D, NKG2A), apoptotic potency of NK cells on BC cells (MCF7) using transwell assay. The IL15 inducer on the NK cell were measured NKG2D, NKG2A gene expression with quantitative polymerase chain reaction (qPCR), (GzmB) secretion using ELISA, apoptotic gene expression of MCF7 using qPCR. IL15 increased NKG2D expression 4.01-9.13%, but IL15 could not affect toward NKG2A expression on NK cells. IL15-activated NK cells, inhibited BC cells proliferation, induced apoptotic BC cells 25.89-32.19%, induced apoptotic genes of BC cells bax, p53. IL15 increase NK activating receptor (NKG2D), inhibit BC cells proliferation, induce apoptotic percentage and induce apoptotic gene expression.
Potential of Conditioned Medium of hATMSCs in Aging Cells Model Wahyu Widowati; Rachmawati Noverina; Wireni Ayuningtyas; Dedy Kurniawan; Seila Arumwardana; Hanna Sari W Kusuma; Rizal Rizal; Dian Ratih Laksmitawati; Ratih Rinendyaputri; Rilianawati Rilianawati; Ahmad Faried
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.378-388

Abstract

Skin aging is caused by the exposure cumulative of ultraviolet radiation, it leads reactive oxygen species (ROS) production in the skin. The conditioned medium of human Adipose Tissue-derived Mesenchymal Stem Cells (hATMSCs) can scavenge free radicals and increase the survival rate of skin cells under oxidative stress. This study examined the protective effects of Conditioned Medium (CM) of hATMSCs in H2O2-induced human skin fibroblast cell line (BJ). The aging cells model using H2O2-induced BJ cells were added CM-hATMSCs in concentrations (0, 10, 30%) and incubated in various time, furthermore BJ cells induced by various H2O2 concentrations (0, 50, 100, 200 µM) incubated for 1 h. The anti-aging potential were measured including viability, ROS and collagen levels in BJ cells which treated CM-hATMSCs. The median inhibitory concentration (IC50) of H2O2 on BJ cells for 1 h incubation was 107.87 μM and 91.25 μM for 10 min incubation. CM-hATMSCs increased the viability on aging model cells. CM-hATMSCs concentration 30% increased the viability of H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs concentration 25% decreased ROS, increased collagen level in H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs increase the viability cells, collagen level and decrease ROS level in aging model cells.
Co-Authors . SETIAWAN Achadiyani Adriani Lawrencia Novalia Afiat Berbudi Agung B Sutiono Agung Budi Sutiono Agung Budi Sutiono Agung Budi Sutiono Agus Dwi Sastrawan Ajeng Diantini Alief Dhuha Alya Mardhotillah Azizah Amelia Ganefianty Amira Puti Karima Angliana Chouw Astrid Tamara Maajid Budiman Bayu W. Putera Benny Atmadja Wirjomartani Benny Isakh Beny Atmadja Wirjomartani Bethy Surjawathy Hernowo Billy Parulian Lubis Bilzardy Ferry Chrismis Novalinda Ginting Cynthia R. Sartika Danny Halim Dedy Kurniawan Derrett, Sarah Dian Ratih Laksmitawati Diana Krisanti Jasaputra Dimas Erlangga Luftimas Dimyati Achmad Dwi A. P. Dewi Dwi Davidson Rihibiha Endang Sjamsudin Ermi Girsang Ervi Afifah Fahmi, Ismail Farid Yudoyono Firman Fuad Wirakusumah Firman Priguna Tjahjono Ganefianty, Amelia Hanna Sari W Kusuma Hanna Sari Widya Kusuma Harry Murti Hasrayanti Agustina Heda Melinda Nataprawira Hendrikus Bolly Hendrikus Masang Ban Bolly Hermin Aminah Usman Hiroshi Koyama I M Kariasa I Nyoman Ehrich Lister Ida Parwati Indah, Astrina Rosaria Indra Bachtiar Ismail Fahmi Jefri Henky Julia Riswandani Julius July Kahdar Wiriadisastra Kalih Sarjono Kariasa, I Made Leri Septiani Leri Septiani Maman Abdurrahman McAllister, Susan MEILINAH HIDAYAT Melia, Regina Mirna Sobana Mochammad Aris Widodo MUCHTAN SUJATNO Muhammad Kusdiansah Muhammad Z. Arifin Muhammad Zafrullah Arifin Mutakin Mutakin Nida Suryana Noverina, Rachmawati NUGRAHA SUTADIPURA Priandana Adya Eka Saputra Priandana Adya Eka Saputra Rachmawati Noverina Ratih Rinendyaputri Renaldi Prasetia Reni Ghrahani Rilianawati Rilianawati Rizal Rizal Rizal Rizal Rizkiditia Nugraha Hadian Rizky Abdulah Robert Sinurat Rully Hanafi Dahlan Sarah Derrett Sarjono, Kalih Seila Arumwardana Sevline Esthetia Ompusunggu Soeprajogo, Adolf Setiabudi Sri Suryanti Subangkit, Mawar Susan McAllister Sutiman Bambang Sumitro Sutiono, Agung B Takayuki Ohira Takeshi Kawase Teresa Liliana Wargasetia The Fransiska Eltania Tono Djuwantono Tono Djuwantono Trajanus Laurens Yembise Tri Hanggono Achmad Wahyu Widowati Wahyu Widowati Wahyu Widowati Wireni Ayuningtyas Yofita Sandra, S.Pd., M.Pd., Zico Farlin, Dr. Budiwirman, M.Pd., Yudi Mulyana Hidayat Yusuf Hariady Hariady Yusuf Sulaeman Effendi