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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
ILG Nurtjahjaningsih
Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta
Agriculture, Biological Sciences & Forestry

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SCREENING PENANDA MIKROSATELIT Shorea curtisii TERHADAP JENIS-JENIS SHOREA PENGHASIL TENGKAWANG ILG Nurtjahjaningsih; AYPBC Widyatmoko; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.1.49-56

Abstract

Screening primer is an effective method to develop microsatellite markers from related taxa. Aim of this study was to develop microsatellite markers of four Shorea producing tengkawang oil, i.e. Shorea gysbertiana, Shorea macrophylla, Shorea pinanga and Shorea stenoptera by screening microsatellite primers of Shorea curtisii. Leaf samples of the four Shorea were collected from nursery at Center for Forest Biotechnology and Tree Improvement Research in Yogyakarta. Four microsatellite primers of S. curtisii i.e. Shc-1, Shc-2, Shc-7 and Shc-9 had been used to screen. Results showed that numerous alleles were shared among the Shorea. The expected heterozygosity (HE) for locus Shc-1 ranged between 0.594 and 0.722; locus Shc-2 ranged between 0.219 and 0.611; locus Shc-7 ranged between 0.594 and 0.778; and locus Shc-9 ranged between 0.594 and 0.844. Coefficient of inbreeding (FIS) value was low and it was insignificant deviated from Hardy-Weinberg Equilibrium (HWE) at  almost all loci except Shc-1 of S.  pinanga. A dendrogram showed two clusters; S. gysbertsiana and S. macrophylla represented in one cluster, while S. pinanga and S. stenoptera represented in another cluster. Therefore the developed microsatellite markers are possible to be applied for studying population genetics and mating system of these species.
PENGEMBANGAN PENANDA MIKROSATELIT PADA Pinus merkusii MENGGUNAKAN METODE DUAL SUPRESSION DAN SCREENING PENANDA ILG Nurtjahjaningsih
Jurnal Pemuliaan Tanaman Hutan Vol 4, No 1 (2010): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2010.4.1.25-35

Abstract

Aims of this study were to develop and characterize microsatellite markers from three species of Pinus i.e P. densiflora, P. Pinaster and P. Taeda were screened to amplify microsatellite in P. merkusii. The results showed that 5 out of the 12 developed microsatellite markers by dual suppression amplified at a single polymorphic locus; otherwise there was no polymorphic locus of the 12 screened microsatellite markers. However, it is difficult to compare the efficiency of these two method because of the limited number of the screened primers used in this study.
KARAKTERISTIK PEMBUNGAAN DAN SISTEM PERKAWINAN NYAMPLUNG (Calophyllum inophyllum) PADA HUTAN TANAMAN DI WATUSIPAT, GUNUNG KIDUL ILG Nurtjahjaningsih; Purnamila Sulistyawati; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 2 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.2.65-78

Abstract

Flowering are influenced by internal factors, such as genetic and phytohormone, and environment factors, such as sunlight and nutrition intake. The flowering characteristics influence fruiting and genetic diversity seedlings through mating systems. This study aims to assess flowering and fruiting characteristics and to determine pattern of mating system of a Calophyllum inophyllum plantation at Watusipat, Gunung Kidul. Flowering and fruiting were observed at 4 locations, 3 parts of crown, and 4 main directions to know the effects of sunlight, nutrition intake and phytohormone in the flowering process. Mating system was assessed by comparing genetic diversity values between parent trees and offsprings. The values of genetic diversity were analyzed using 5 RAPD primers with 17 polymorphic loci. Analysis of variant showed that the locations, crown parts, directions and interaction between a location and direction significantly affected to differences number of flowers and fruits. Values of genetic diversity (h) of parent trees ranged between 0.1471 and 0.3056. The values increased at almost overall offsprings; it ranged between 0.2864 and 0.3750. Values of genetic distance (Da) between parent trees were high and very high (0.197 – 0.364), but the values was decreased between parent trees and their offspring, even between offspring populations. A dendrogram showed two main clusters; first cluster consisted parent trees at up edge with rare trees and second cluster consisted sub cluster parent trees at up edge; sub cluster parent trees at down middle; and sub cluster parent trees at down edge and overall offsprings. Flowering/ fruiting characteristics and pattern of mating systems of C. inophyllum were briefly discussed. Key words : Flowering characteristic, genetic diversity, Calophyllum inophyllum, RAPD analysis
KERAGAMAN GENETIK ANAKAN Shorea leprosula BERDASARKAN PENANDA MIKROSATELIT Purnamila Sulistyawati; AYPBC Widyatmoko; ILG Nurtjahjaningsih
Jurnal Pemuliaan Tanaman Hutan Vol 8, No 3 (2014): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2014.8.3.171-183

Abstract

Genetic diversity value of offspring might indicate a reproductive success in a forest. Aim in this study was to access genetic diversity values of offspring of Shorea leprosula from different forest types. Leaf samples were collected from six population i.e. a plantation from Carita, and five natural forests from Gunung Bunga A and B, SBK, ITCI and Gunung Lumut. Using four microsatellite markers, the results showed that SBK population maintained high value of genetic diversity. Values of expected heterozygosity (HE) ranged between 0.717 (Carita) and 0.836 (SBK). Values of coefficient inbreeding (F) were insignificant deviated from Hardy-Weinberg Equilibrium, but the value was significant in SBK population. PCA analysis demonstrated a cluster among Gunung Bunga A, SBK, ITCI, and Gunung Lumut. Amova showed that different province significantly contributed 1% to the value of genetic diversity of S. leprosula.
Co-Authors Anto Rimbawanto Anto Rimbawanto AYPBC Widyatmoko Purnamila Sulistyawati