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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
Anto Rimbawanto
Centre For Forest Biotechnology and Tree Improvement Research and Development Yogyakarta
Agriculture, Biological Sciences & Forestry

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THE USE OF FTA CARD ON DNA SAMPLE PREPARATION FOR MOLECULAR OF PLANT DISEASE IDENTIFICATION Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 1, No 3 (2007): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2007.1.3.123-130

Abstract

Accurate and guick identification of pathogen is key to control the spread of plant disesases. Morphological identification is often ineffective because  it requires fruit body which often are not presence, rely on characters which may be highly variable within and among species and can be slow and time consuming. Molecular identification of plant disease can overcome most of the shortcomings of morphological identification. Application of FTA Cardn for sample collection is crucial for the success of molecular identification of phatogen. The study present an earlier results on the suitability of FTA Card to collect sample in situ. The study was done on 4 different sample namely pure culture of fusarium sp., infected leaf, soil and seeds which assumed to have mycelium. The result indicate that FTA Card is effective as a collecting media of pathogen for most samples, paticularly for mycelium culture. Furthur experiments are still required to ensure the suitability of the card for leaf, seeds and soil samples.
ESTIMATION OF GENETIC DIVERSITY WITHIN AND AMONG PULAI (Alstonia scholaris (L.) R. Rr.) PROVENANCE REVEALED BY RAPD MARKER Dwi Hartati; Anto Rimbawanto; Taryono Taryono; Endang Sulistyaningsih; AYPBC Widyatmoko
Jurnal Pemuliaan Tanaman Hutan Vol 1, No 2 (2007): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2007.1.2.89-98

Abstract

Pulai (Alstonia seholaris (L.) R. Br.) is species of high economic value and has been intensive utilization. Conservation effort and breeding strategies should be carried out prevent extinetion. The study ofgenetic diversity using RAPD marker can asses polymorphism through bandingpatterns from amplified DNA. The aims of this research are to estimate genetic diversity within and among pulai populations, investigate distribution ofgenetic diversity, and genetic relationship between pulai provenance. Leaf samples were taken from eighteen pulai provenances in Indonesia, namely Lubuk Linggau, Pendopo, Benakat, Banten, Bantul, Gunungkidul, Bali, Purworejo, Perawang, Mataram, Sumbawa, Kupang, Timor Tengah Selatan, Agam, Gowa, Makassar, and Kendari. Genetic diversity was analyzed using 23 primers and produced 114 polymorphic loci. Results showed that the distribution ofgenetic diversity within provenance was higher than that of among provenance. Cluster analysis revealed that the eighteen provenances was into two major groups. The first group consisted of provertance Lubuk Linggau, Banten and Pendopo. The second group of provenance of Benakat, Perawang, Agam, Solok, Bali, Kendari, Bantul, Purworejo, Gunungkidul, Mataram, Sumbawa, Gowa, Makassar, Kupang, and Timor Tengah Selatan. In general, the genefic relationships among eighteen provenances donot show the relation between genetic diversity and geographic distribution of pulai provenance.
FLOWERING AND FRUITING STUDY OF Melaleuca cajuputi subsp. cajuputi Powell AT PALIYAN SEEDLING SEED ORCHARD, GUNUNGKIDUL, YOGYAKARTA Liliana Baskorowati; R Umiyati; Noor Khomsah Kartikawati; Anto Rimbawanto; Mudji Susanto
Jurnal Pemuliaan Tanaman Hutan Vol 2, No 2 (2008): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2008.2.2.189-202

Abstract

Observation on of flowering and fruiting phenology  were undertaken at M. cajuputi subsp. Cajuputi seedling seed orchard Paliyan, Gunungkidul from February to October 2002. Seed viability was tested out at Forest Biotechnology and Tree Improvement Research Institute, Yogyakarta. The objectives of this study are: (1) to identify the flowering time, (2) to determine the time of buds development and (3) to observe the reproductive success and the number of germination seeds per capsule of open pollination seeds. The study showed that peak flowering of M. cajuputi subsp cajuputi at Paliyan seedling seed orchard occurred in March; several trees at this population also flowered during February to June. The buds initiation to capsules maturity took on average of 277 days. Three months after fertilisation, the capsules were ready to harvest. Mean number of reproductive success of open pollination seeds is 3.6%. The mean number of seed viability varied between provenance range from 6 to 32 seeds/capsule.
Co-Authors AYPBC Widyatmoko Dwi Hartati Endang Sulistyaningsih Liliana Baskorowati Mudji Susanto Noor Khomsah Kartikawati Purnamila Sulistyawati R Umiyati Taryono, Taryono