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All Journal HAYATI Journal of Biosciences VALENSI Jurnal Natur Indonesia Jurnal Kimia Riset Jurnal Zarah Science and Technology Indonesia Jurnal Farmasi Sains dan Komunitas (JFSK) ALCHEMY Jurnal Penelitian Kimia Molekul: Jurnal Ilmiah Kimia SAP (Susunan Artikel Pendidikan) JURNAL LENTERA BISNIS Jurnal Natural Darma Sabha Cendekia Molekul: Jurnal Ilmiah Kimia
AMIN FATONI
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Jenderal Soedirman, Purwokerto, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Social Sciences

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MENGIDENTIFIKASI PANDANGAN NATURE OF SCIENCE (VNOS) CALON GURU KIMIA Yulita, Inelda; Adriani, Nina; Fatoni, Amin; Hermawan, Dadan; Mudzakir, Ahmad
Jurnal Zarah Vol 7 No 2 (2019): Jurnal Zarah
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (648.418 KB) | DOI: 10.31629/zarah.v7i2.1550

Abstract

Sangat penting untuk mengetahui pandangan calon guru kimia tentang Nature of Science karena akan mempengaruhi kinerja disaat menjadi guru yang sesungguhnya. Guru yang memiliki pandangan mengenai nature of science (VNOS) yang benar, akan mentransfer kemampuan tersebut kepada para siswanya, begitu juga sebaliknya pemahaman yang salah juga akan tertularkan kepada para siswa. Aspek VNOS yang akan dibahas meliputi (1) Tentativeness, (2) Empirical basis, (3)Subjectivity, (4) Creativity, (5) Social/cultural embeddedness, dan (6) Theories and laws. Metode yang digunakan dalam penelitian ini adalah deskriptif. Instrumen yang digunakan dalam penelitian ini adalah kuisioner VNOS form B yang telah diadopsi dari Lederman dan pedoman wawancara. Pandangan calon guru kimia dijelaskan dan dikategorikan sebagai naïve, eclectic, dan informed untuk setiap aspek NOS. Hasil penelitian menunjukkan bahwa calon guru kimia memiliki pandangan yang cukup memadai tentang Nature of Science.
HUBUNGAN KUALITAS PELAYANAN DENGAN KEPERCAYAAN MAHASISWA Fatoni, Amin
JURNAL LENTERA BISNIS Vol 8, No 2 (2019): JURNAL LENTERA BISNIS
Publisher : POLITEKNIK LP3I JAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34127/jrlab.v8i2.313

Abstract

The Relationship between Service Quality with the Student's Trusty. The  objective of this research to know the lecturer’s and education service with competence, guarante of work fast and education service with student’s trusty in Polytechnic LP3I Jakarta Pasar Minggu campus. The research is carried out in campus Pasar Minggu due to the efficiency of time and place of respondent. The method of this research is by using descriptive statistic and hypothesis test. Base on the relation of service qualiy with students trusttuidance for the managerial, mainly in upgrading the service quality in term of upgrading the student's recommendation. The purpose of this research is to exa, it will result: lecturer’s competences have the significant relation eith student’s trusty. Promotion of guarantee of work fast has the significant relation with the students trusty, education service has the significant relation with the student’s trusty.Keyword : Studnts’s trusty, Lecturer’s Competence, Education Service
Isolasi, Pemurnian dan Karakterisasi Lipase Bakteri Hasil Skrining dari Tanah Tempat Pembuangan Akhir (TPA) Gunung Tugel Banyumas Zusfahair, Zusfahair; Setyaningtyas, Tien; Fatoni, Amin
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.835 KB) | DOI: 10.31258/jnat.12.2.124-129

Abstract

A bacterial lipase producer was isolated from garbage dump soil and was identified its genus. Lipase was extractedaccording to production time optimized, purified using ammonium sulfate fractionation and gel chromatograph.Determination of enzyme characteristic studied were influence of pH, temperature, various metals to lipaseactivity. The result of this research shows that the genus of isolated bacteria which produced lipase wasAcinetobacter sp., the lipase optimum production time is about 18 hours with the activity is about 115 unit/mL. Thehighest activity of lipase fractionation using ammonium sulfate is about 45% and the highest activity of purifyingwith filtration gel chromatograph column using Sephadex G-150 at 24 th fraction. Lipase from crude extract andpurifying product at this fraction has optimum pH 6 and optimum temperature is about 40 oC. Lipase to be classifiedas metalloenzyme that shows with decreasing the activity after added the EDTA. Metals ion, such as Cu 2+ and Zn2+were inhibited the lipase activity. Ca 2+ ion could increase lipase crude extract activity but inhibited the activity oflipase purifying product. Hg2+ ion could increase the activity of lipase purifying product.
Isolasi dan Karakterisasi Protease Ekstraseluler dari Bakteri dalam Limbah Cair Tahu Fatoni, Amin; Zusfahair, Zusfahair; Lestari, Puji
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.484 KB) | DOI: 10.31258/jnat.10.2.83-88

Abstract

Protease has been used in large application industrial process such as detergent, leather, textil, softdrink, andmedicine. In order to find unique protease, many substances were explored as proteases of bacteria sources. Inthis study, tofu liquid waste was used as a source of bacteria producing proteases. Waste sample was growth inskim milk agar medium showing proteases activity, it was used to produce extracellular protease. The microbialcolonies were identified as Staphyllococcus sp. Protease was extracted with 5000 g centrifugation at 4 0C, andpurificated with ammonium sulphate precipitation continued with dialisis. Optimum production time, pH, metal ion,EDTA, specific activity, KM, and Vmaks were studied for enzyme characterization. Volume of crude enzyme was 300ml, with spesific activity of 3.55 U/mg. Protease obtained from 60% ammonium sulphate fraction had the highestspecific activity of 68.22 U/mg. Study on the protease characterization revealed that optimum temperature of thisenzyme was 400C. The optimum pH of the enzyme was found to be 8.0. The kinetic parameters K M dan Vmaks withcasein as substrate were 0.31% and 51.55 U/ml. Some inhibitory effect was observed in the presence of EDTA, Cu +2,Co+2, Zn+2, and enzyme activity was stimulated by Mg+2, indicating that this ion had a functional role in the molecularstructure of the enzyme.
Hidrolisis Pati Ganyong (Canna edulis) dengan Amilase Bakteri Flavobacterium sp. PTBT I untuk Produksi Bioetanol Ningsih, Dian Riana; Zusfahair, Zusfahair; Fatoni, Amin
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.385 KB) | DOI: 10.31258/jnat.15.2.92-98

Abstract

Bioethanol is an alternative energy of fuels produced from vegetable materials. Vegetable materials that can be used as rawmaterial for bioethanol is ganyong because it contains 22.60 g starch in 100 g ganyong. The production of bioethanol fromstarch material consisted of two steps, hydrolysis and fermentation. One of the steps to increase the value of bioethanolfrom starch of ganyong was hydrolysis process using thermostable amylase enzyme isolated from Flavoacterium sp.PTBT I bacteria was isolated from hot spring of Pancuran Tujuh Baturraden. The aim of this research was to use thermostableamylase to hydrolyze starch of ganyong and glucose produced to result bioethanol. The result of this research showed thatthe optimum condition hydrolysis starch of ganyong was using thermostable amylase acquired at substrate concentrationof 3% (b/v), and incubation time of about 75 minutes. The value of bioethanol increased with time of fermentation, from thefirst to fourth day, which was 0.8361; 2.2379; 5.7590 and 10.5787% (v/v), respectively.
Backmatter (Instruction for author and back cover) Fatoni, Amin
Molekul Vol 15, No 3 (2020)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.586 KB) | DOI: 10.20884/1.jm.2020.15.3.743

Abstract

Backmatter (Instruction for author and back cover) of the print version
Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents) Fatoni, Amin
Molekul Vol 16, No 1 (2021)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.797 KB) | DOI: 10.20884/1.jm.2021.16.1.769

Abstract

Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents)
Determination of Cu and Pb concentrations based on urease activity inhibition of Durio zibethinus L. seeds Zusfahair, Zusfahair; Fatoni, Amin; Ningsih, Dian Riana; Riapanitra, Anung
Molekul Vol 16, No 2 (2021)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.71 KB) | DOI: 10.20884/1.jm.2021.16.2.736

Abstract

The determination of heavy metal concentrations has been carried out using sophisticated instruments, and therefore a simple and reliable alternative method is needed as a comparison. The study aimed to determine Cu and Pb concentration of standard solution using the urease activity inhibition method of Durio zibethinus L.  seeds.  The research started with urease extraction from Durio D. zibethinus L. seeds. The activity of the obtained extract was determined using the Nessler method. The optimum substrate concentration was also determined. Urease activity inhibition was carried out using various metal solution concentrations, which continued by plotting a log graph of urea concentration vs. %inhibition. The obtained graph would then determine the metal concentration in a synthetic water sample. The data was then compared to the measurement, determined by the Atomic Absorption Spectrophotometry (AAS) method. Results of the study showed that the urease activity of D. zibethinus L.seeds was 296.774 U/mL. Urease activity was optimum at a urea concentration of 0.3 M. The comparison Cu, and Pb concentration determination using the urease inhibitory activity and AAS methods showed no significant difference at 95% confidence level. This research showed that urease of D. zibethinus L. seed could be used to determine Cu and Pb's concentration based on its inhibiting activity.
Antibacterial Activity of Propolis Produced by Trigona spp. Against Campylobacter spp. AMIN FATONI; I MADE ARTIKA; AHMAD ENDANG ZAINAL HASAN; KUSWANDI KUSWANDI
HAYATI Journal of Biosciences Vol. 15 No. 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.266 KB) | DOI: 10.4308/hjb.15.4.161

Abstract

Propolis is believed to have antimicrobial, anti-inflammatory and immunostimulating activities. The objective of this study was to investigate the antibacterial activity of ethanol extract propolis (EEP) of Trigona spp. from Bukittinggi West Sumatera against Campylobacter spp. Antibacterial activity of the EEP was measured by disc diffusion method. The compound groups of the propolis were also analyzed on the existence of alkaloids, flavonoids, saphonins, tannins, steroids, and terpenoids. This study revealed that the EEP of Trigona spp. shows an antibacterial activity on Campylobacter spp. The compound groups detected in the EEP were flavonoids and tannins, suggesting that the antibacterial activity of propolis of Trigona spp. may be due to these compounds. Key words: Trigona spp., antibacterial activity, Campylobacter spp
Aplikasi Urease dari Biji Kacang Tolo (Vigna unguiculata ssp unguiculata L.) untuk Biosensor Urea zusfahair zusfahair; Dian Riana Ningsih; Amin Fatoni; Vika Aprilia Puspitarini
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 5, No. 1, May 2019
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (669.044 KB) | DOI: 10.15408/jkv.v5i1.8776

Abstract

Penggunaan urease dalam analisis urea yang digabungkan dengan suatu transduser disebut biosensor urea.Tujuan penelitian adalah menentukan kadar urea dengan metode biosensor urea berbasis urease biji kacang tolo yang diamobilisasi pada matrik alginat dan dideteksi secara kolorimetri menggunakan indikator bromtimol biru. Penelitian dimulai dengan isolasi urease dari biji kacang tolo (Vigna unguiculata ssp unguiculata L.), kemudian diamobilisasi menggunakan metode penjebakan dengan natrium alginat, setelah mencampur larutan urease dengan natrium alginat, diteteskan dalam larutan CaCl2 sampai terbentuk urease alginat. Beads urease alginat direaksikan dengan urea menghasilkan ion amonium, selanjutnya ditambahkan indikator bromtimol biru dan perubahan warnanya diukur menggunakan spektrofotometer. Kinerja analitis biosensor urea ditentukan melalui penentuan waktu reaksi enzimatis, keberulangan analisis, keberulangan pembuatan dan pengujian senyawa penganggu dengan konsentrasi urea 4mM, serta penentuan linearitas, batas deteksi, dan batas kuantifikasi dengan konsentrasi urea 0.05; 1; 3; 7; dan 15 mM. Hasil penelitian menunjukkan beads urease alginat bisa digunakan berulang sampai 8 kali. Kinerja analitis beads urease alginat menghasilkan respon yang linier pada rentang 0.05-15 mM dengan koefisien korelasi sebesar 0.9981, batas deteksi sebesar 0.8 mM dan batas kuantifikasi sebesar 2.67 mM. Keberulangan pembuatan beads urease alginat menghasilkan nilai koefisien variasi sebesar 6%. Analisis tidak terganggu dengan keberadaan asam askobat 0.05 mM dan asam urat 0.4 mM. Kata kunci: Amobilisasi urease, beads alginat, biosensor, biji kacang tolo, spektrofotometri. The use of urease in the urea analysis which combined with a transducer is called urea biosensor. Research aimed to determine urea level using urea biosensor method based on urease from black-eyed pea that immobilized on alginate matrix and detected by colorimetric using bromothymol blue indicator. The research began with urease isolation from black-eyed pea (Vigna unguiculata ssp unguiculata L.), and then it immobilized utilizing the trapping method with sodium alginate, after mixing urease solution with sodium alginate, it is dripped in CaCl2 solution until alginate urease beads formed. Alginate urease beads reacted with urea to produce ammonium ion, then it’s added with indicator bromothymol blue, and the color changes were measured using a spectrophotometer. ­The analytical performance of urea biosensor is determined by enzymatic reaction time, repeated analysis, repeatability of fabrication and calibration of disturbing compound with concentration of urea 4 mM, also linearity, limit of detection and limit of quantification with concentration of urea 0.05, 1, 3, 7 and 15 mM. The results showed that alginate urease beads could repeatedly be used until eight times. The analytical performance of alginate urease beads including a linear response in the range of 0.05 mM-15 mM with the correlation coefficient of 0.9981, the detection limit of 0.8 mM and the quantification limit of 2.67 mM. The repeatability of fabrication alginate urease beads produced the coefficient of variation value of 6%. The presence of 0.05 mM ascorbic acid and 0.4 mM uric acid.was not disrupted the analysis. Keywords: urease immobilization, alginate beads, biosensor, black-eyed pea, spectrophotometry.
Co-Authors A.A. Ketut Agung Cahyawan W AHMAD ENDANG ZAINAL HASAN Ahmad Mudzakir Ahmad Mudzakir Anung Riapanitra Anung Riapanitra Ari Asnani Cacu Cacu Dadan Hermawan Dadan Hermawan Dadan Hermawan Dadan Hermawan, Dadan Darul Santri Pertiwi Dian Riana Ningsih DIAN RIANA NINGSIH Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dwi Agustina V Dwi Kartika Elok Dwi Putri Lestari ELY SETIAWAN Hartiwi Diastuti Hassan Y. Aboul-Enein HENDRI WASITO I MADE ARTIKA Indah Permatawati Khansa Salsabila Mala Nurilmala Mando Hastuti Mardiyah Kurniasih Mekar Dwi Anggraeni Mekar Dwi Anggraeni Mekar Dwi Anggraeni Mudasir Mudasir Nina Adriani Nina Adriani Ponco Iswanto Suwandri Suwandri Suwandri Suwandri Suyata Suyata Tien Setyaningtyas Uyi Sulaeman Vika Aprilia Puspitarini Vonia Febriana Hidayah Warsinah Warsinah Yulia Yulia Yulita, Inelda Zusfahair Zusfahair Zusfahair Zusfahair Zusfahair Zusfahair ZUSFAHAIR ZUSFAHAIR Zusfahair Zusfahair Zusfahair Zusfahair Zusfahair Zusfahair zusfahair zusfahair Zusfahair Zusfahair Zusfahair Zusfahair, Zusfahair