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All Journal Biogenesis: Jurnal Ilmiah Biologi
Ikhsan Nur Huda
Laboratorium Genetika, Fakultas Biologi, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Analisis Variasi Genetik Melon (Cucumis melo L.) Kultivar Gama Melon Basket Dengan Metode Random Amplified Polymorphic DNA Ikhsan Nur Huda; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 1 No 1 (2013)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v1i1.446

Abstract

Melon (Cucumis melo L.) cultivars Gama Melon Basket (GMB) is the result of plant breeding that has the potential to be a product of local melon seed in Indonesia. This cultivar phenotype characters was known, but its genetic variation is not further studied. This study aimed to analyze the genetic variation of melon cultivars GMB and compared with 13 commercial melons and four members of the family Cucurbitaceae such as; cucumber, cucumber queen, barteh, and cantaloupe (blewah). RAPD-PCR method with 11 random primer was used to evaluate genetic variation in melon. Level of genetic similarity was determined by the formula simple matching coefficient (SSM) and the dendogram constructed by clustering analysis unweighted pair-group using arithmeticaverages (UPGMA). Results of RAPD-PCR with 11 primer produced 165 loci and it contains 84.85 % polymorphic DNA and 15.15 % monomorphic DNA. Results of genetic variation analysis suggests that 15 commercial melons differ from groups of cucumber, cucumber queen, barteh and cantaloupe(blewah). Cultivars GMB has genetic marker that can be distinguished from other commercial melons. GMB cultivar have specific polymorphic DNA on the size of 285 bp generated by PMAR primer.Keywords: Cucumis melo L., Gama Melon Basket, genetic variation, RAPD
Co-Authors BUDI SETIADI DARYONO