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All Journal Jurnal Fitopatologi Indonesia Jurnal Hortikultura
Erniawati Diningsih
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Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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Metode Pengakaran Batang Bawah Mawar Bebas Prunus Necrotic Ringspot Virus Secara In Vitro Erniawati Diningsih; Fitri Rahmawati; Yoyo Sulyo; - Darliah
Jurnal Hortikultura Vol 19, No 4 (2009): Desember 2009
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v19n4.2009.p%p

Abstract

Metode untuk memproduksi batang bawah mawar bebas virus sudah diperoleh pada penelitiansebelumnya. Untuk mendapatkan bibit bebas virus diperlukan metode pengakaran yang tepat secara in vitro. Pengakaranmerupakan salah satu tahap penting dalam teknik kultur jaringan untuk perbanyakan bibit tanaman secara cepat.Penelitian bertujuan mendapatkan jenis media terbaik untuk pengakaran batang bawah mawar bebas virus. Penelitiandilaksanakan di Laboratorium Virologi dan Kebun Percobaan Balai Penelitian Tanaman Hias, Segunung, Cianjur, JawaBarat (1.100 m dpl), dari bulan Januari sampai Desember 2003. Percobaan menggunakan rancangan acak kelompokpola faktorial dengan 3 ulangan. Faktor pertama adalah 3 kultivar batang bawah mawar bebas virus (Rosa multiflora,Rosa sp. cv. Multic, dan cv. Natal Brior). Faktor kedua adalah 7 komposisi media pengakaran (MS+IBA 0,5 mg/l,MS+IBA 1,0 mg/l, MS+NAA 0,5 mg/l, MS+NAA 1,0 mg/l, MS+IAA 0,5 mg/l, MS+IAA 1,0 mg/l, dan MSO (tanpaZPT)). Hasil penelitian menunjukkan bahwa media pengakaran yang paling baik untuk memproduksi batang bawahmawar bebas virus yaitu dengan komposisi MS+IBA 0,5 mg/ml. Implikasi hasil penelitian ini adalah tersedianyateknologi pengakaran in vitro untuk batang bawah mawarABSTRACT. Diningsih, E., F. Rahmawati, Y. Sulyo, and Darliah. 2009. In Vitro Rooting Method for ProducingFree-Virus Rose Rootstock. The method for producing virus free plantlet of rose rootstock had been obtained inprevious study. In obtaining virus-free seed, availability of appropriate in vitro rooting method is needed. Rootingis one of the important step in tissue culture technique for plant rapid multiplication. The purpose of this experimentwas to obtain the best rooting medium for producing virus-free rose rootstock. The experiment was conducted in theVirology Laboratory of the Indonesian Ornamental Crop Research Institute, Segunung, Cianjur, West Java (1,100 masl.) from January to December 2003. The research was laid in a factorial randomized block design with 3 replications.The first factor was 3 virus-free rose rootstock cultivars (Rosa multiflora, Rosa sp. cv. Multic, and cv. Natal Brior)resulted from the previous experiment. The second factor was 7 compositions of medium (MS+IBA 0.5 mg/l, MS+IBA1.0 mg/l, MS+NAA 0.5 mg/l, MS+NAA 1.0 mg/l, MS+IAA 0.5 mg/l, MS+IAA 1.0 mg/l, and MSO (without PGR)).The results showed that the best rooting medium for producing virus-free rose rootstock was MS+IBA 0.5 mg/l.
Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum Erniawati Diningsih; Safani Aryantika; Indijarto Budi Rahardjo; Wakiah Nuryani; Hanudin; Ifa Manzila
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023 (IN PROGRESS)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.196-205

Abstract

Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum Chrysanthemum stunt viroid has been widely reported to be a limiting factor in the production of chrysanthemum cut flowers. The aim of this study was to determine the effect of low temperature treatment (5 ℃) and antiviral ribavirin on the growth and elimination of Chrysanthemum stunt viroid (CSVd) in chrysanthemum plantlets cultured from shoot tips. CSVd infected plants were obtained from a government experimental garden in Cianjur, West Java. The research was carried out in several stages, namely 1) plant sampling, 2) viroid detection using RT-PCR method, 3) initiation of explants and propagation of plant material in vitro, 4) low temperature treatment 5 ℃ with three levels of incubation time at 1, 3, and 5 months, 5) ribavirin antiviral treatment with three levels of concentration (25, 100, and 125 ppm), and 6) confirmation of viroid-free plant materials by RT-PCR. The results showed that low temperature storage and ribavirin treatment reduced the rate of growth of shoot height and number of leaves. Ribavirin did not cause phytotoxity up to concentration level of 125 ppm, although it caused inhibition on shoot growth and leaf number. However, the combination of low temperature treatment (1, 3, and 5 months) with ribavirin (25, 100, and 125 ppm) was not able to eliminate CSVd from tissues at all treatment levels.
Co-Authors - Darliah FITRI RAHMAWATI Hanudin Ifa Manzila Indijarto Budi Rahardjo Safani Aryantika Wakiah Nuryani Yoyo Sulyo