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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Jurnal Kimia (Journal of Chemistry) METAMORFOSA Journal of Biological Sciences Buletin Veteriner Udayana Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS Biotropika BULETIN FISIKA Cassowary Jurnal Sewaka Bhakti Widyanatya: Jurnal Pendidikan Agama dan Seni
Ida Bagus Gede Darmayasa
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Veterinary Other

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Bioactive Compound from Extract Filtrat Streptomyces sp.Sp1. as Biocontrol of Vibriosis on Larvae of Macrobrachium rosenbergii shrimps Retno Kawuri; IBG Darmayasa
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (913.24 KB) | DOI: 10.4308/hjb.26.1.15

Abstract

  Production of Macrobrachium rosenbergii shrimp was limited by vibriosis disease. Streptomyces can be used as an alternative control of vibriosis which is caused by Vibrio anguillarum. Aplication of Streptomyces sp.1 culture could give significant different impacts (p<0.05) on the percentage of survival (SR) of shrimp larvae that had been infected by V. anguillarum compared to the control. This study was conducted to identify and determine bioactive compound of Streptomyces sp.1. The presence of bioactive compound was confirmed by gas chromatography-mass spectrometry (GC-MS) analysis. Identification of Streptomyces sp.1 by 16S rRNA partial gene sequencing method. Streptomyces sp.1 was identified to be Streptomyces sp.Sp1. There were 35 chemical compounds identified, the chemical compounds which have antimicrobial properties identified in the n butanol extract of Streptomyces sp.Sp1 are 3-Hexanone, 2-methyl (43%), n Butyl ether (19%) Nonane (CAS) n-Nonane (5.6%), Decane (CAS) n-Decane (3.2%), Hexacosane (CAS) n-Hexacosane (1.9%), Tetracontane (1.3%), Heneicosane (1.9%), Hexadeconoic acid, methyl ester (0.3%), Butane,1,1-dibutoxy (0.3%), and Limonene (0.3%). Benzeneacetic acid, 3-methoxy-.alpha.,4-bis[(tri (0.3%). These results suggested that Streptomyces sp.Sp1 had good general antimicroba activity and might have potential biocontrol antagonist Vibrio anguillarum.
KARAKTERISASI FRAKSI AKTIF ANTIBAKTERI EKSTRAK DARI DAUN PEPE (Gymnema reticulatum Br) I M. Dira Swantara; I. B. Darmayasa; Sri lestari
Jurnal Kimia (Journal of Chemistry) Vol. 4, No. 2 Juli 2010
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Isolation and caracterisation of antibacterial compounds in the leaves pepe have been carried out. Extractionwas carried out by macerating using methanol and 70%. Separation of extracts which have activity to Eschericia coliand Stathylococcus aereus was conducted using the solvent partition by petroleum ether, chloroform, and ethylacetate. The most active extract, the ethyl acetate extract was separated by column chromatography using a mixtureeluent of chloroform, and ethyl acetate with a ratio of 1:1. The eluents were tested for their bioactivity asantibacterial agent and the results established that fraction 1 was the most active fraction. This next wing fraction 1was then identified using GC-MS.The gas chromatogram showed 3 peaks at 18.658 minutes (33.69%), 19.617 minutes (34.01%), and 25.492minutes (32.69%). It was suggested that the fraction contains three compounds. The first peak has a molecularion atm/z 296 with a base peak of 68, matches with the spectra of 3,7,11,15-tetramethyl 2-hexadesene-1-ol. The secondpeak, m/z 270 and 74, matches with the spectra of metyl hexadecanoate ester. The last peak has amoleculer ion at390 and base peak at 149, suggesting it is dioctyl-1,2-benzenedicarboxylilate.
UJI AKTIVITAS ANTIBAKTERI FRAKSI KULIT BATANG NANGKA I Made Dira Swantara; Ida Bagus Gede Darmayasa; Ni Komang Ayu Kumala Dewi
Jurnal Kimia (Journal of Chemistry) Vol. 5, No. 1 Januari 2011
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

This research aims to isolate and identify antibacterial compounds in jackfruit bar husk (Artocarpus heterophyllus, Lam.). 735.55 gramc of jackfruit bar husk yielded 500,25 grams of crude methanol.extract. The methanol extract was fractionated using petroleum ether, ethyl acetate and of n-butanol and then evaporated to yield crude extract of ethyl acetate 4.11 grams of petroleum ether (yellowish), 2.11 grams of ethyl acetate extract (brownish), and 1.17 grams of n-butanol (dark green).Bioactivitigs tests to Staphylococcus aureus and Eschericia coli showed that ethyl acetate extract had the largest inhibition zone. The purification of ethyl acetate extract with column chromatography resulted a faction which was antibacterial active (faction C).Identification of faction C using Gas Chromatography Mass Spectroscopy showed 3 compounds namely methyl hexadecanoate, dioctyl hexadioate, and dioctyl-1,2-benzene dicarboxylate.
Eliminasi Escherichia coli O157:H7 Yang Diisolasi Dari Daging Sapi Di Rumah Potong Hewan (RPH) Dan Pasar Tradisional Ni Wayan Purni wirathi; Retno Kawuri; Ida Bagus Darmayasa
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p08

Abstract

This research was conducted to determine the quality of beef in slaughterhouses and Traditional markets in Denpasar, Badung, and Klungkung, in terms of Escherichia coli and isolation E. coli O157:H7 which are suspected of contaminating beef. Experimental research was conducted in a laboratory with a heating test of E. coli O157: H7 to determine the heat resistance of E. coli O157: H7. Beef taken at Nyanggelan, Panjer market shows the highest value of E. coli which is 222.3 colonies/g, and the lowest value of E. coli is obtained in beef taken at Kaliunda abattoir which is 2.3 colonies/g. The identification results on 39 beef samples showed that all beef samples contained E. coli, 24 samples positive of E. coli O157, and 9 other samples showed positive E. coli O157:H7. The heating treatment at 60? still grows E. coli O157:H7 colonies in the Petri dish, but there has been a decrease in the number of initial colonies before the heating process. All locations were 100% contaminated with E. coli, 61.5% positive E. coli O157, and 25.6% positive E. coli O157:H7. Heating 65? for 15 seconds and 70? for 5 seconds can kill E. coli O157: H7 in beef. Keywords: E. coli O157: H7, beef, slaughterhouses, traditional markets, heating process.
Elusidasi Awal Daya Hambat Ekstrak Etanol Daun Ketapang (Terminalia Catappa L.) Terhadap Pertumbuhan Staphylococcus Aureus ATCC25923 Penyebab Gingivitis Putu Pande Purwaningsih; Ida Bagus Darmayasa; Ni Putu Adriani Astiti
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p08

Abstract

The leaf of “Ketapang” (Terminalia catappa L.) can be used as traditional medicine. The use of natural material is generally considered to be safer and has less side effect as compared to the use of modern medicine. This study aims at analysing the early elucidation of resistor property of the ethanol extract of the “ketapang” leaf against the growth of Staphylococcus aureus ATCC25923 as the cause of gingivitis, and to identify the active compound contained in the “ketapang” leaf. This study uses Complete Random Design (RAL) with 7 treatments of the extract concentration of “ketapang” leaf namely concentration of 1%, 2%,3%, 4%, 5%, positive control (chlorexidine) and negative control (ethanol). The discovery of the resistor efficacy of “ketapang” leaf extract against S. aureus bacteria using Kirby Beuer method has been indicated by the development of clear zone as the result of the application of anti-bacteria compound which is diffused in bacteria growth media. The finding of the study shows that the higher the concentration of the extract (5%), the wider the development of the zone of the resistor area. The effectiveness value of the anti-bacteria of “ketapang” leaf extract is not better than the antiseptic being tested. The result of profile test of phytochemical test shows that the extract of “ketapang” leaf contains tannin, saponin, terpenoid and flavonoid. The result of GC-MS test, it has been identified five compounds being contained in fraction IV namely Alpha Terpinolene, Cyclohexanol 5-methyl, Hexadecanoic acid, Octadecanoic acid, 1.2Benzenedicarboxylic acid. five active compounds that we found have the potentials of functioning as anti-bacteria.
Potential of Awar-awar Leaf Methanol Extract (Ficus septica Burm. f.) Against Aspergillus niger on Balinese Lontar Ni Komang Deny Julyeda; Ida Bagus Gde Darmayasa; Junita Hardini
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p10

Abstract

Ficus septica Burm. f. is a wild plant that has antifungal compounds. Exploration of antifungal compounds from natural material important in inhibiting the growth of Aspergillus niger, one of Balinese lontar destructive fungus. Natural material use to reduse lontar damage due to synthetic materials. This study aims to inhibition of awar-awar leaf methanol extract (in vitro). The method used was a complete randomized design with 7 treatments for the concentration of the awar-awar leaf methanolic extract of 10.000, 20.000, 30.000, 40.000, 50.000 ppm (b/v), positive control (nystatin), and negative control (methanol). The minimum inhibitory concentration (MIC) test uses 6 treatments for the concentration of the awar-awar leaf methanolic extract of 100, 500, 1000, 2000 ppm (w/v), positive control (nystatin), and negative control (methanol). Giving the extract to the diffusion well with potato dextrose agar (PDA) media. The research data were analyzed statistically ANOVA (DMRT with 5% significance level) with the SPSS 23 version for windows. The results showed that the awar-awar leaf methanol extract had MIC at a concentration of 500 ppm (w/v). Concentrations of 10.000-50.000 ppm (w/v) indicate the inhibition zone diameter of 20.50 mm, respectively; 23.00 mm; 23.50 mm; 23.75 mm; 24.75 mm. The concentration of 10.000 ppm (w/v) has higher inhibition than the positive control. The concentration of 20.000 ppm (w/v) was the optimum concentration of inhibition of awar-awar leaf methanol extract. Conclusion of this research was awar-awar leaf methanol extract had anti-fungal compounds which inhibited the growth of A. niger. This extract can be used as an alternative to inhibit the growth of fungi that cause lontar damage. Keyword: Aspergillus niger, destructive fungi, Ficus septica Burm. f.
Elusidasi Dan Daya Hambat Ekstrak Etanol Daun Sembung Delan (Sphaeranthus indicus L.) Terhadap Candida albicans ATCC 1023 Yulita Salma Lani; Ida Bagus Gde Darmayasa; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p18

Abstract

The sembung delan (Sphaeranthus indicus L.) plant is one of the weeds in Indonesia which has great potential as an alternative antifungal. The purpose of this study was to determine the ability of sembung delan leaf extract to inhibit the fungus Candida albicans ATCC 1023, as a pathogenic species and cause of candidiasis, as well as chemical compounds contained in the extract. The research design used in this study was a completely randomized design (CRD) divided into 7 treatments and 4 repetitions with one treatment factor, namely the concentration of 1% (w/v), 2% (w/v), 3% (w/v), 4% (w/v), 5% (w/v). Positive control (Nystatin) and negative control (Solvent). Phytochemical tests were carried out and analysis of compound content using GC-MS. The results showed that sembung delan leaf extract was able to inhibit C. albicans ATCC 1023 with the greatest inhibition, namely at a concentration of 5% (w/v) with a diameter of 13.00 mm, a minimum concentration of 0.3% (w/v) with a diameter of 5.925 mm. Statistically significantly different (P?0.05) with a concentration of 0.1% (w / v), and 0.2% (w / v) with a diameter of 0.000 mm and an optimum concentration of 4% (w/v) with a diameter of 11.25 mm. Phytochemical test results of the crude extract of sembung delan contain saponins, phenols, steroids, terpenoids, alkaloids, and tannins. For GC-MS analysis, 7 active compounds were obtained with 6 of them known as anti-fungal compounds, namely Thymol; 1-Tetradecanamine, N, N-dimethyl-; Benzene, 2-methoxy-1,2,3-trimethyl-; 6-Octenal, 3,7-dimethyl-; Thymol and Benzyl chloride. The active compounds contained in sembung delan leaf extract have the opportunity to be used as herbal ingredients to control C. albicans ATCC 1023. Keywords: Minimum Inhibitory Concentration, phytochemicals, thin layer chromatography, gas chromatography mass spectrometry.
Potensi Ekstrak Daun Dewandaru (Eugenia uniflora L.) Sebagai Pengendali Bakteri Bacillus cereus ATCC 11778 Penyebab Diare Putu Cindy Arista; Retno Kawuri; I.B. Gede Darmayasa
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p16

Abstract

Eugenia uniflora L. is a medical plant that can be used as a medicine for diarrhea. Exploration of antibacterial compounds from natural material can resolve various diseases that do not give effects of resistance such as the use of antibiotics. Research purposes are to know the potential of dewandaru leaf extract as an agent bacteria control of Bacillus cereus ATCC 11778 that causes diarrhea and to determine the active compounds contained in dewandaru leaf extract. The method used in this study was a Complete Randomized Design (CRD) with 7 treatments for the concentration of the dewandaru leaf extract of 1%, 2%, 3%, 4%, 5% (b/v), positive control (amoxicillin), and negative control (ethanol). Determination of inhibition of dewandaru leaf extract againts B. cereus ATCC 11778 is characterized by the formation of the clear zone around paper discs using the Kirby-Bauer method. The results showed that dewandaru leaf extract at the highest concentration (5%) was able to produce inhibition zone diameter of 7.15 mm against B. cereus ATCC 11778. Phytochemical test results showed ethanol extract dewandaru leaf contain tannin, saponin, terpenoid, and flavonoid as active compounds. GCMS analysis of the active fraction of dewandaru leaf extract (Fraction II) produced ten antibacterial compounds. The conclusion of this study, ethanol extract of dewandaru leaf was able to inhibit the growth of B. cereus ATCC 11778 in vitro. Concentration dewandaru leaf extract 1% is a minimal concentration as an antibacterial againts B. cereus ATCC 11778. GCMS analysis of dewandaru leaf extract consists of 10 antibacterial compounds such as Dodecanoic acid, Tridecanoic acid, Hexadecanoic acid, Octadecanoid acid, 8-Nitro-11-dodecanolide, Oxiraneoctanoic acid, Oxiraneoctanoic acid, 9-Octadecenoic acid, 9-Octadecenoic acid, and 1.2-Benzenedicarboxylic acid. Keyword: Antibacterial, Inhibition zone, Active Compounds.
Optimasi Periode Kultur Vibrio cholerae Ogawa pada Medium BHIB untuk Meningkatkan Daya Simpan Kultur Suta Arta; Retno Kawuri; Ida Bagus Gde Darmayasa
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p04

Abstract

Balai Besar Pengawas Obat dan Makanan (BBPOM) berkewajiban untuk menguji setiap produk pangan dari cemaran bakteri patogen sebelum diedarkan untuk mencegah terjadinya wabah penyakit yang ditularkan lewat makanan termasuk salah satunya Vibrio cholerae.Dalam proses pengujian produk pangan terhadap cemaran V. cholerae, terdapat masalah dalam hal ketahanan hidup kultur kerja V. cholerae.Penelitian ini merupakan penelitian eksperimental laboratorik dengan menggunakan Rancangan Acak Lengkap(RAL) Pola Faktorial.Tujuan dari penelitian ini untuk mendapatkan waktu pertumbuhan maksimal kultur V. cholerae Ogawa pada media (Brain Heart Infusion Broth)BHIB pada suhu 37oC serta untuk mendapatkan kombinasi derajat keasaman (pH) dan konsentrasiNaCl yang tepat pada medium BHIB untuk meningkatkan umur kultur kerja V. cholerae Ogawa yang disimpan pada suhu kulkas (4-8oC). Penelitian diawali dengan re-identifikasi isolat kemudian dilakukan uji kurva tumbuh dengan inkubasi 37oC selama 15 jam pada medium BHIB. Setelah diperoleh pola pertumbuhannya pada medium BHIB, penelitian dilanjutkan dengan menguji ketahanan V. cholerae Ogawa pada medium BHIB yang dimodifikasi dengan kombinasi pH (5,6,7,8, dan 9) dan konsentrasi NaCl (0,1,2, dan 3%) pada penyimpanan suhu kulkas (4-8oC). Hasil penelitian uji kurva tumbuh pada medium BHIB menunjukkan bahwa fase log (pertumbuhan maksimal) terjadi pada jam ke-5. Hasil uji ketahanan pada medium BHIB yang dimodifikasi dengan kombinasi pH dan konsentrasi NaCl menunjukkan bahwa kombinasi pH 9 dan NaCl 3% merupakan kombinasi terbaik untuk meningkatkan umur daya simpan V. cholerae Ogawa pada medium BHIB. Pada kombinasi tersebut V. cholerae Ogawa mampu bertahan sampai hari ke-51 pada penyimpanan suhu kulkas (4-8oC).
Isolasi, Identifikasi dan Persentase Keberadaan Hifa Jamur Endofit pada Tanaman Gemitir (Tagetes erecta L.) di Beberapa Daerah di Bali Bekti Revi Rahayu; Meitini Wahyuni Proborini; Ida Bagus Gede Darmayasa
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p12

Abstract

Endophytic fungi are found in roots, stems, leaves, flowers, fruit, until rhizosphere in soil area, where the fungus is widely used as an antifungal, biostimulan, antibacterial, and many more. Gemitir is one plant which widely cultivated in some areas in Bali. In Bali this plants use as a material for upakara, ornamental plants and herbs. This study was conducted to determine the type and different kind of endophytic fungi and to see the percentage of endophytic fungal hyphae in the roots, stems, and leaves on gemitir which taken at different locations. Sampling was taken at the Pangsan-Badung, Angseri-Tabanan Regency and Temesi-Gianyar. The sampling method which used is the diagonal method with five sample points. Isolation and identification process was carried out in the Laboratory of Plant Taxonomy (Mycology), State University of Udayana in January-March 2017. In this study of 15 species successfully identified to the genus level that belongs to the six genera namely Alternaria, Aspergillus, Botrytis, Trichoderma, Penicillium and Syncepalastrum. Total of fungal colonies in most samples were found at research location A (Pangsan-Badung Village) and percentage of highest endophytic fungal hypha existed at location C (Temesi-Gianyar Village).
Co-Authors A.A.S. A. Sukmaningsih Alayna Himawan Alfian Siswanto Anak Agung Gede Indraningrat Ariyanti Ni Kadek Ayu Amelia Bekti Revi Rahayu Cokorda Putra Deny Christine Sidabutar Dwi Dirgantini, Anak Agung Faldy Efriant Pangemanan Fania Agustini Flavia Fionessa Falenda Shanzet I Gede Sugita Artana I Gusti Agung Ayu Ratnawati I Gusti Ayu Laras Pratama Putri I Ketut Gede Rudita I KETUT MUKSIN I Ketut Sundra I Komang Adi Widyastama I Luh Putu Wiwin Astari I Made Dira Swantara I Made Sara Wijana I Made Sugiarta I Nyoman Winyana I W BUDIARSA SUYASA I Wayan Kasa I Wayan Sudiarsa I Wayan Sukadana I.P.G. Ardhana Ida Ayu Gde Yadnyawati Ida Bagus Made Suaskara Ilham . Joko Wiryatno Junita Hardini Kadek Feby Widyantari Kadek Sudarmi Komang Krisna Lalu Muhammad Sakti Surya Jagat Made Ria Defiani MADE SUSUN PARWANAYONI Martin Joni Meitini Proborini Wahyuni Nadia Khairunnisa Nasha Hadi Rahmadanty NI KADEK WIWIK SINTA DEWI Ni Komang Ayu Kumala Dewi Ni Komang Deny Julyeda Ni LUH ARPIWI Ni Luh Putu Eka Suartini Ni Made Indah Suandewi Ni Putu Adriani Astiti Ni Putu Indiani Sahaning Karna Ni Putu Sinta Puspa Dewi Ni Putu Yuliatiningsih Ni Wayan Purni wirathi Ni Wayan Sudatri Nyoman Mega Antari Pande Gde Eka Mardiana Pande Gde Sasmita Julyantoro Pande Putu Christine Putri Purnami Pande Putu Purwaningsih Prayitna Dewi, Ida Ayu Putu Cindy Arista Putu Eka Sudaryatma Retno Kawuri Rochmalia Juniarti Putri Sang Ayu Sri Satya Laksmi Utari SANG KETUT SUDIRGA Siti Didah Alawiah Siti Nurlatifah SRI LESTARI Suasthi, I Gusti Ayu Suta Arta Yan Ramona Yulita Salma Lani