Johanes C. Mose
Departemen Obstetri Dan Ginekologi, Fakultas Kedokteran, Universitas Padjadjaran, Bandung.

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KORELASI ANTARA EKSPRESI AROMATASE, FOLLICLE STIMULATING HORMONE (FSH), DAN INSULIN-LIKE GROWTH FACTOR (IGF)-I DENGAN MATURITAS OOSIT PADA PELAYANAN TEKNOLOGI REPRODUKSI BERBANTU (TRB) Assangga Guyansyah; Achmad Biben; Johanes C. Mose; Bethy Suryawathy Hernowo
Indonesian Journal of Applied Sciences Vol 2, No 1 (2012)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.032 KB) | DOI: 10.24198/ijas.v2i1.2729

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Karakteristik biokimia cairan folikel disekitar oosit memainkan peran penting dalam menentukan kualitas oosit yang padaakhirnya dapat meningkatkan keberhasilan program TRB. Secara umum mekanisme maturitas oosit yang dipengaruhi oleh unsur kimia cairan folikel melibatkan IGF-I yang bekerja dengan melipatgandakan efek FSHR, lalu menginduksi peningkatan kadar cAMP intraseluler yang merupakan mediasi utama stimulasi FSH pada ekspresi aromatase. Penelitian ini merupakan studi analitik korelasional dengan pendekatan crossectional pada pasien yang mengikuti program TRB di Klinik Aster Rumah Sakit Dr. Hasan Sadikin Bandung dalam kurun waktu Januari 2011 sampai dengan Februari 2012. Analisis hasil dilakukan menggunakan uji Shapiro Wilk, uji Wilcoxon, uji Mann-Whitney, uji ANAVA, rank Spearman, dan uji chi-kuadrat. Kemaknaan hasil uji akan ditentukan berdasarkan nilai p < 0,05. Pada penelitian ini didapatkan perbandingan jumlah oosit matur dan imatur pada ekspresi FSH kuat menunjukkan ada perbedaan yang bermakna (p=0,03). Analisis hubungan antara ekspresi IGF-I dengan maturitas oosit menunjukkan hasil yang tidak bermakna secara statistik (p=0,192). Didapatkan pula median proporsi prevalensimaturitas sebesar 0,71. Maturitas oosit dengan median rasio prevalensi > 0,71 pada ekspresi FSH kuat besarnya 1,71 kali bila dibandingkan dengan ekspresi FSH negatif. Pada ekspresi aromatase sedang dengan median rasio prevalensi maturitas > 0,71 sebesar 1,55 kali bila dibandingkan dengan ekspresi aromatase yang negatif.
The correlation between Leptin Levels and Onset of Preeclampsia Roza Sriyanti; Johanes C. Mose; Masrul Masrul; Netti Suharti
Andalas Obstetrics And Gynecology Journal Vol 4, No 2 (2020)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.4.2.139-144.2020

Abstract

The purpose of this study is to find the correlation between leptin levels and the onset of preeclampsi. This study used a cross sectional comparative study design that conducted in May 2018 - April 2019 in the SMF / Obstetrics and Gynecology department of RSUP dr. M. Djamil Padang, RSUD Achmad Mochtar, RSUD Solok, RST Reksodiwiryo. We used consecutive sampling method which consists of 69 pregnant women who fulfill the inclusion and exclusion criteria. Leptin level tests were done using ELISA method. The average level of leptin in early-onset preeclampsia is found to be the highest when compared to the late-onset preeclampsia and normal pregnancy, 64.07 ± 78.27 vs. 30.46 ± 31.99 vs. 16.61 ± 24.49. This differentiation is highly significant with the ANOVA statistical test (p <0.05). There is a significant correlation between leptin levels with the onset of preeclampsia.Keywords: preeclampsia early onset, preeclampsia late onset, leptin levels
IL-12 PE, CD 69 PERCP, CD3 FITC, AND CD4 APC OPTIMIZATION WITH ACTIVATION OF ISOLATED AGENT HEAT-KILLED SONICATED MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN RINI SUNDARI; IDA PARWATI; JOHANES C. MOSE; BUDI SETIABUDIAWAN
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1676.676 KB)

Abstract

Infection caused by Mycobaterium tuberculosis exists in form of intracellular infection, which leads to lymphocyte activation. CD69 is the first lymphocyte activation marker expressed in Th1 lymphocyte, which follows by IL-12 release. Flow cytometry analysis can identify the subpopulations of lymphocytes and  intracellular cytokines such as IL-12, yet precise preparation needs to be done. This research aims to conduct optimization with four color lyse/wash flow cytometry assay system FastImmune™ FACSCalibur examination, with monoclonal antibody IL-12, CD69, CD3, and CD4 in succession uses fluorochrome PE, PerCP, FITC, and APC.To activate the lymphocytes from heparinized whole blood, we used activation agent which derives from isolated heat-killed sonicated Mycobacterium tuberculosis Beijing strain. Optimal concentration from the according activation agents is 40 mL. To determine the compensation, BDTM CompBead and blank-cell unstainning are used, but the maximum result showed by blank-cell unstainning.Each monoclonal antibody dosage of IL-12PE, CD69 PerCP, and CD3 FITC is 40 mL, while CD4 APC 5 mL. Total event lymphocyte is determined minimally by 10,000 events. With 18,510 total events and Th gated events quantity are 4,692, the result obtained is IL12-PE has 7.4% gated (347 events); CD69+ perCP/CD3+ FITC 18.2% (850 events); and CD69+ perCP/CD4+ APC 3.9%.
Pengaruh Likopen terhadap Kadar Soluble FMS-Like Tyrosine Kinase 1 pada Preeklamsi Vaulinne Basyir; Prima Nanda Fauziah; Akhmad Yogi Pramatirta; Yanwirasti Yanwirasti; Johanes C. Mose; Fadil Oenzil
Jurnal Kesehatan Andalas Vol 9, No 4 (2020): Online December 2020
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v9i4.1513

Abstract

Preeklamsi merupakan penyebab utama morbiditas dan mortalitas baik maternal maupun perinatal. Tujuan: Menganalisis pengaruh pemberian likopen terhadap kadar sFlt-1 secara in vitro pada sel trofoblas model preeklamsi. Metode: Penelitian ini dilakukan secara eksperimental di Laboratorium Genetika Molekuler dan Kultur Sel Fakultas Kedokteran Universitas Padjadjaran selama periode 2017-2018. Sampel serum diperoleh dari RS. Dr. Hasan Sadikin Bandung, sesuai kriteria inklusi dan eksklusi. Kriteria inklusi adalah pasien yang telah didiagnosis preeklamsi berdasarkan pemeriksaan tekanan darah dan protein urin, dan kriteria eksklusi adalag pasien yang tidak datang kontrol atau tidak bisa dihubungi. Penelitian ini terdiri dari dua tahap, pertama ditentukan nilai LD50 likopen terhadap sel trofoblas kemudian dijadikan dasar nilai konsentrasi yang akan digunakan, selanjutnya dilakukan pemeriksaan sFlt-1 menggunakan metode Elisa pada kultur sel trofoblas kondisi normal dan preeklamsi dengan analisa data menggunakan analisis varians (Anova) dan uji Duncan untuk mengetahui perbedaan antar variabel. Hasil: Nilai LD50 adalah 250 ug/ml dan serial konsentrasi yang digunakan mulai dari 1,953 sampai dengan 125 ug/ml. Didapatkan kadar sFlt-1 menurun secara signifikan (p0,05) dari 39,5445 menjadi 31,1342 pg/ml pada sel trofoblas yang diinduksi dengan serum preeklamsi setelah pemberian konsentrasi likopen sebesar 15,625-31,25 ug/ml yang nilainya mendekati keadaan pada sel trofoblas yang diinduksi serum kehamilan normal. Pemberian likopen dapat menurunkan kadar sFlt-1 pada sel trofoblas preeklamsi. Simpulan: Likopen dapat merubah kadar sFlt-1 dan perubahannya dipengaruhi oleh konsentrasi dan jenis serum.Kata kunci: likopen, preeklampsi, sFlt-1 
Correlation between fms-Like Tyrosine Kinase-1 (sFlt-1) Cell-Free Messenger RNA Expression and fms-Like Tyrosine Kinase-1 (sFlt-1) Protein Level in Severe Preeclampsia and Normal Pregnancy Jeffry Iman Gurnadi; Johanes Cornelius Mose; Budi Handono; Prima Nanda Fauziah; Akhmad Yogi Pramatirta
International Journal of Integrated Health Sciences Vol 3, No 2 (2015)
Publisher : Faculty of Medicine Universitas Padjadjaran

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Abstract

Objective: Preeclampsia is still a problem in Indonesia due to the high maternal and neonatal morbidity and mortality. Placental soluble fms-like tyrosine kinase-1 (sFlt-1), an antagonist of vascular endothelial growth factor (VEGF), and placental growth factor (PIGF), is considered as one of the etiology factors that cause endothelial damage in preeclampsia due to increased sFlt-1 level modulating vascular endothelial integrity. This study aims to analyze sFlt-1 and sFlt-1 mRNA levels in severe preeclampsia and normal pregnancy, and the correlation between both in occurrence of severe preeclampsia.Methods: This is a cross-sectional analytic observational study involving 18 subjects with severe preeclampsia and 19 subjects with normal pregnancy as controls who met inclusion and exclusion criteria. Levels of sFlt-1 and sFlt-1 mRNA were measured with ELISA and RT PCR. Statistical analysis was performed with Chi square test, Fisher’s exact test, T-test, Mann-Whitney test, and Spearman’s rank correlation test.Results: This study showed no significant difference (p>0.05) in characteristics of maternal age, gestational age, and parity in both study groups. Mean level of sFlt-1 mRNA in severe preeclampsia was higher (6.3404 pg/mL) compared to its level in normal pregnancy (5.9701 pg/mL). There is a not significant (p>0.05) positive correlation between sFlt-1 mRNA with sFlt-1 level in normal pregnancy, and a not significant (p>0.05) negative correlation between both level in severe preeclampsia.Conclusions: sFlt-1 mRNA levels in severe preeclampsia are higher than its level in normal pregnancy. There is no correlation between sFlt-1 mRNA level and sFLt-1 protein level in severe preeclampsia. There is a not significant positive correlation between sFlt-1 mRNA with sFlt-1 level in normal pregnancy, and a not significant negative correlation between both in severe preeclampsia.Keywords: Normal pregnancy, severe preeclampsia, sFlt-1 mRNA DOI: 10.15850/ijihs.v3n2.586
Sindrom Metabolik: Komplikasi Pertumbuhan Janin Intrauterin yang Terhambat Adhi Pribadi; Johanes C. Mose
Majalah Kedokteran UKI Vol. 30 No. 2 (2014): APRIL - JUNI
Publisher : Fakultas Kedokteran Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/mkvol34iss2pp60

Abstract

Abstrak Perkembangan manusia intrauterin berkorelasi dengan penyakit yang timbul pada periode dewasa. Hipotesis perkembangan menyatakan bahwa risiko suatu penyakit dalam jangka panjang secara inisial diinduksi respons adaptasi janin. Respons termasuk perubahan metabolisme dan hormon, yang mungkin berpengaruh terhadap pertumbuhan organ. Penelitian epidemiologi menunjukkan BBLR dan PJT berhubungan dengan penyakit jantung, Stroke, DM tipe 2, obesitas, Sindrom Metabolik, dan osteoporosis. Periode perikonsepsi merupakan masa yang sensitif terhadap perubahan dan defisiensi nutrisi. Memori isyarat lingkungan terekam sejak konsepsi sampai persalinan oleh janin dan diturunkan pada generasi berikutnya. Hal ini meningkatkan dugaan bahwa penyakit metabolik diturunkan berbasis epigenetik dan tidak murni berbasis multigenik.Kata Kunci: intrauterin, perkembangan, PJT, sindrom metabolik Abstract Adult period diseases correlate with intrauterine development. Hypothesis development notes that the risk in latter life is induced by intrauterine fetal adaptive responses. The responses include: mal-development of metabolism and hormone that can influence alter fetal organs development. Result of epidemiology study stated that IUGR has significant correlation with cardiac disease, stroke attack, Diabetes Mellitus type 2, obesity, metabolic syndrome, and osteoporosis. Periconception period is a sensitive period of change and nutrition deficiency. Environment memory has been recorded by the fetus since conception until fetal delivery and has consequences to be transfered to the offspring. This increases the assumption that metabolic diseases signal have epigenetic memory base and not merely multigenic base.Kay Words: intrauterine, development, IUGR, metabolic syndrome
The Correlation of L-citrulline Levels with Blood Pressure in Severe Preeclampsia Hudila Rifa Karmia; Afriwardi Afriwardi; Hirowati Ali; Johanes Cornelius Mose; Yusrawati Yusrawati
The Indonesian Biomedical Journal Vol 12, No 1 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i1.964

Abstract

BACKGROUND: Based on 'vascular disorder of pregnancy' terminology, preeclampsia primarily was not a hypertensive disorder, but a vascular disorder (general vasospasm) in pregnancy due to idiopathic etiology. The overall incidence of preeclampsia was 5-14% of all pregnancies. One of the substances responsible for regulating vascular tone is nitric oxide (NO), which produced in endothelial blood vessels. NO and L-citrulline are produced altogether by the reaction between L-arginine and oxygen. L-citrulline levels reflected NO production. This study was aimed to assess the correlation between the L-citrulline level and blood pressure in severe preeclampsia.METHODS: This cross-sectional study was done in Dr. M. Djamil Central General Hospital, Padang, Indonesia The sample size was 36 samples of pregnant women with severe preeclampsia and had yet been given antihypertensive therapy. Sampling was done by consecutive sampling from Obstetrics and Gynecology Division. After maternal examination and measurements L-citrulline levels of cubital venous blood by using enzyme-linked immunosorbent assay (ELISA) method, Pearson correlation was performed to assess the relationship between variables for normally distributed data and Spearman's correlation for abnormal distribution data with significance level p<0.05.RESULTS: Means of L-citrulline levels, systolic blood pressure, diastolic blood pressure, and arterial pressure (MAP) were 87.21 nmol/mL, 179.4 mmHg, 108.3mmHg, and 132.1 mmHg, respectively. Correlation of the L-citrulline level with systolic blood pressure, diastolic blood pressure, and MAP were -0.08, -0.175, and -0.136 (p>0.05), respectively.CONCLUSION: L-citrulline levels had no correlation with blood pressure in severe preeclampsia.KEYWORDS: L-citrulline levels, blood pressure, severe preeclampsia
MMP-9 Level Comparation between Spontaneous Abortion and Normal Pregnancy Aloysius Suryawan; Johanes C Mose; Budi Handono
The Indonesian Biomedical Journal Vol 4, No 3 (2012)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v4i3.178

Abstract

BACKGROUND: Abortion remains a problem in the obstetric care as it is one of the causes of maternal and fetal death. Bleeding or miscarriage threat in the first trimester comprises 20% to 25% of all pregnant women, and 50% of them will end as spontaneous abortion. The major cause of maternal death in abortion is hemorrhage. Some recent studies have indicated that MMP-9 is important in the process of embryo implantation into the endometrium and spontaneous abortion occurs when there is an overwhelming increase of MMP-9. This data indicate the importance of a further research to elucidate the role of MMP-9 in spontaneous abortion.METHODS: This was a cross sectional study, included 70 pregnant women with gestational age <20 weeks who came for examination and treatment at the Department of Obstetrics and Gynecology, Medical Faculty of Padjadjaran University/Hasan Sadikin Hospital, Bandung. RESULTS: There were differences in the MMP-9 levels in spontaneous abortion compared to normal pregnancy and MMP-9 had a cut-off point >1221.7 with the sensitivity of 48.6% specificity of 80%, and accuracy of 60.9%.SUMMARY: The MMP-9 level in spontaneous abortion was higher than in normal pregnancy.KEYWORDS: MMP-9, spontaneous abortion, normal pregnancy
Kadar Calprotectin pada Bayi Kurang Bulan dan Respiratory Distress Syndrome Ali Usman; Abdurachman Sukadi; Johanes Cornelius Mose
Global Medical & Health Communication (GMHC) Vol 1, No 2 (2013)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1819.23 KB) | DOI: 10.29313/gmhc.v1i2.1522

Abstract

Calprotectin merupakan peptida antimikrob yang disimpan dalam leukosit sebagai molekul efektor dari respons imun innate. Terdapat infeksi intrauterin/intraamnion berhubungan dengan peningkatan calprotectin, defensin, dan bacterial permeability increasing protein (BPI) secara bermakna di dalam cairan amnion pada persalinan kurang bulan. Penelitian ini bertujuan untuk mengetahui kadar calprotectin pada bayi kurang bulan yang menderita dan tidak menderita respiratory distress syndrome (RDS). Penelitian observasional analitik dilakukan selama periode Maret 2010–Maret 2012 di RS Dr. Hasan Sadikin, RS Advent, RS Al Islam, RS Khusus Ibu dan Anak Kota Bandung, dan RS Ibu Anak Hermina Pasteur, diperoleh sampel sebanyak 60 bayi kurang bulan yang menderita dan tidak menderita RDS masing-masing sejumlah 30 orang. Cairan amnion diambil untuk pemeriksaan kadar calprotectin. Diagnosis RDS dibuat berdasarkan pemeriksaan fisis, skor Downe, dan foto toraks. Didapatkan nilai median kadar calprotectin pada kelompok bayi kurang bulan yang menderita RDS 3.302,6 sedangkan pada kelompok bayi yang tidak menderita RDS 7.908,85. Pada uji Mann-Whitney diperoleh Zm-w=3,063 (p=0,002). Cut-off point (COP) calprotectin >3.395,6 didapatkan jumlah RDS (+) 17 dan RDS (-) 4, sedangkan pada COP calprotectin >3.395,6 jumlah RDS (+) 13 dan RDS (-) 26. Dapat disimpulkan, kadar calprotectin pada bayi yang menderita RDS berbeda dibandingkan dengan kadar calprotectin pada bayi yang tidak menderita RDS. Kadar calprotectin dapat memprediksi kejadian RDS dengan sensitivitas 56,7%; spesifisitas 86,7%; dan akurasi 71,7%. CALPROTECTIN CONCENTRATIONS IN PRETERM NEONATES  AND RESPIRATORY DISTRESS SYNDROMECalprotectin is antimicrobial peptides stored in leucocytes, that acts as effector molecules of the innate immune response. Intrauterine or intraamniotic infection was associated with a significant increase in amniotic fluid concentration of calprotectin, defencin and bacterial permeability increasing protein (BPI). The study aims to explore calprotectin concentrations at preterm neonates with and without respiratory distress syndrome. An observational analytic study was performed during March 2010-March 2012 in Dr. Hasan Sadikin, Advent, Al Islam, Bandung Mother and Child and Hermina Pasteur Hospitals. Subjects were 60 preterm neonates who divided in two groups, 30 neonates with and 30 neonates without RDS. Sixty samples of amniotic fluid were collected to examine calprotectin concentration. The diagnoses of RDS were made based on physical examination, Downe score and chest X-rays. Median value of calprotectin concentrations from preterm neonates with RDS were 3,302.6 and neonates without RDS were 7,908.85, with Mann-Whitney test Zm-w=3.063 (p=0.002). Cut off point (COP) of calprotectin 3,395.6 in RDS (+) were 17 and in RDS (-) were 4. Calprotectin in RDS COP >3,395.6 (+) were 13 and RDS (-) were 26. In conclusion, the concentration of calprotectin at RDS (+) is very low and significantly different with the one  at RDS (-). The concentration of calprotectin can be used to predict the incidence of RDS with sensitivity 56.7%, specificity 86.7% and accuracy 71.7%.
The Effect of the BC-MK15 Birth Chair on the Labor Pain Intensity in Multipara Yetti Purnama; Johanes Cornelius Mose; Herry Herman
Global Medical & Health Communication (GMHC) Vol 6, No 3 (2018)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.576 KB) | DOI: 10.29313/gmhc.v6i3.2841

Abstract

Labor pain causes worry, anxiety, increases the secretion of the adrenaline, adrenocorticotropic hormone (ACTH), cortisol serum levels, catecholamines and the perception of pain. One method to decrease labor pain is through the mobilization and upright position during labor which is facilitated by the BC-MK15 birth chair to relax, decrease catecholamines, increase the release of β-endorphins and block the transmission of pain stimulus. This study aims was analyze the difference of the effect of using the BC-MK15 birth chair and the conventional bed on the intensity of labor pain in multipara. The design of this study was the posttest-only control group design in 60 samples of the first active phase of multipara at Public Health Centers Garuda, Ibrahim Adjie and Puter of Bandung city in April–May 2017. Each group consisted of 30 for treatment (using BC-MK15 birth chair) and control (using the conventional bed). Assessment of pain scores using the visual analogue scale (VAS) in cervical dilations of 4 cm (post 1), 7–9 cm (post 2) and 10 cm (post 3). The statistical test analysis uses the Mann-Whitney test and the chi-square test. There were significant differences among groups which are obtained by the treatment and control of post 2 and post 3 (p<0.05, RR=0.69 [0.531–0.896]). In conclusion, there is an effect of using the BC-MK15 birth chair and the conventional bed on the intensity of labor pain in multiparous mothers. PENGARUH KURSI PERSALINAN BC-MK15 TERHADAP INTENSITAS NYERI PERSALINAN PADA MULTIPARANyeri persalinan menimbulkan rasa khawatir, kecemasan, meningkatkan sekresi adrenalin, adrenocorticotropic hormone (ACTH), kadar kortisol serum, katekolamin, dan persepsi nyeri. Salah satu metode menurunkan nyeri persalinan adalah mobilisasi dan posisi tegak saat persalinan difasilitasi dengan kursi persalinan BC-MK15 sehingga menimbulkan relaksasi, menurunkan katekolamin, meningkatkan pelepasan β-endorfin, dan mengeblok transmisi stimulus nyeri. Penelitian ini bertujuan menganalisis perbedaan pengaruh penggunaan kursi persalinan BC-MK15 dan tempat tidur konvensional terhadap intensitas nyeri persalinan pada multipara. Desain penelitian eksperimen posttest-only control group design  dengan jumlah sampel 60 multipara kala I fase aktif di Puskesmas Garuda, Ibrahim Adjie, dan Puter Kota Bandung pada bulan April–Mei 2017. Tiap-tiap kelompok berjumlah 30 untuk perlakuan (menggunakan kursi persalinan BC-MK15) dan kontrol (menggunakan tempat tidur konvensional). Penilaian skor nyeri menggunakan visual analogue scale (VAS) pada dilatasi serviks 4 cm (post 1), 7–9 cm (post 2), dan 10 cm (post 3). Pengujian statistik menggunakan analisis Uji Mann-Whitney dan uji chi-square. Didapatkan perbedaan bermakna antara kelompok perlakuan dan kontrol pada post 2 dan post 3 (p<0,05; RR=0,69 [0,531–0,896]). Simpulan, terdapat pengaruh penggunaan kursi persalinan BC-MK15 dan tempat tidur konvensional terhadap intensitas nyeri persalinan pada ibu multipara.