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SELEKSI PRIMER UNTUK ANALISIS KERAGAMAN GENETIK JENIS BITTI (Vitex coffassus) . Gusmiaty; Muh. Restu; Ira Pongtuluran
PERENNIAL Vol. 8 No. 1 (2012)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v8i1.211

Abstract

The successful of genomic DNA amplification using RAPD technique was determined by the sequence of primer’s base and primer compound in each reaction. The aim of research was finding the best primer for genetic diversity analyses of Bitti (Vitex coffassus). The results of amplification showed that number of band between one to five bands (110-600 bp). The primer of OPK-10, OPA-17, OPQ-07 and OPP-08 can be used for genetic diversity analyses of Bitti. The best primer used for genetic diversity analyses of Bitti was OPP-08 because it had the highest number of bands (5 bands). Key words: OPP-08, Primer Selection, Vitex coffassus
PENGARUH DOSIS INOKULAN ALAMI (EKTOMIKORIZA) TERHADAP PERTUMBUHAN SEMAI TENGKAWANG (Shorea pinanga) . Gusmiaty; Muh. Restu; Ayu Lestari
PERENNIAL Vol. 8 No. 2 (2012)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v8i2.217

Abstract

This research aimed to know the effect of some doses of natural inoculants on the growth of Shorea pinanga seedling. The research was carried out with several steps including seed provision, inoculation of ectomycorrhyza, planting and maintenance in the nursery. The result of the research showed that height and number of branches were different at the significant level of 5 %, while diameter and number of leaves were not significantly different. The natural inoculants dose of 15 g showed the best growth in response to the increment of height, diameter, and number of leaves, while that of 20 g showed the best in the number of branches increment. Key words: Shorea pinanga, ectomycorrhyza, seedling, natural inoculantstor, Local Government Revenue, regional autonomy