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Sri Hartati H
Sebelas Maret University School of Medicine, Solo

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Perbedaan Kadar Kuersetin pada Propolis di Pasaran Wilayah Surakarta P, Rizky Amalia; Prasetyo, Diding Heri; H, Sri Hartati
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Quercetin is one of the active substances in propolis which has many benefits for human health as antioxidant, anticancer, antiallergy, cardioprotector, gastroprotector, lowering blood pressure, and increase the body immunity. Quercetin content in propolis is influenced by geographical factors, differences in the season, climate, plants, bees, and the type of solvent. This study aims to determine differences of quercetin levels of propolis in the market area of ​​Surakarta. Methods: This study uses observational analytic methods. Samples of propolis were sold in the market area of ​​Surakarta. Sampling was done by purposive sampling as many as six brands of propolis which is divided into 6 groups: P1, P2, P3, P4, P5, and P6. Each group was measured five times. Determination of quercetin in propolis by using UV-Vis spectrophotometer method of Prussian Blue. Data were analyzed for quercetin levels with one-way ANOVA statistical test using the computer program SPSS 17 for windows. Results: Average of quercetin levels of propolis samples obtained results P1 = 9.4440 ± 0.06465 µg/mL, P2 = 4.4760 ± 0.08591 µg/mL, P3 = 10.2700 ± 0.04000 µg/mL, P4 = 7.4120 ± 0.06648 µg/mL, P5 = 8.6780 ± 0.03347 µg/mL, P6 = 8.4960 ± 0.10877 µg/mL with  p < 0.001. Conclusion: There are differences of quercetin levels of propolis in the market area of ​​Surakarta. Keywords: Propolis, quercetin levels, UV-Vis spectrophotometer, Prussian Blue 
Perbedaan Kadar Kuersetin pada Propolis Ekstrak Etanol dan Propolis Ekstrak Air Yulianti, Nurrini Susanti; Prasetyo, Diding Heri; H, Sri Hartati
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.858 KB)

Abstract

Background: Propolis contains flavonoids. One of them is quersetin.  Solvent used is propolis extraction influence quercetin level of the extract. This research aims to determine whether there is a difference of Quercetin Level in Ethanol Extract Propolis and Water Extract Propolis. Methods: This research is an experimental research. Subject of this research is propolis from Gejen RT 3 RW 2, Kerjo, Karanganyar. The samples were carried out by purposive sampling. Concentration of quercetin is determined by using UV-Vis spectrophotometer by Prussian-blue methode. Five samples are made for each extract. The data then analized using unpaired t-test by SPSS 17 for windows. Results: Averages of quercetin level  in this research   are 10,0480±0,53798 µg/mL in Ethanol extract propolis and 1,0440±0,06804 µg/mL in Water Extract propolis with p < 0,05. Conclusions: There are differences in there is a difference of quercetin content in Ethanol Extract Propolis and Water Extract Propolis. Keywords: Propolis, Quercetin level, UV-Vis spectrophotometer, Prussian-blue