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Pengaruh Suhu terhadap Perkembangan Pradewasa Parasitoid Eriborus argenteopilosus Cameron (Hymenoptera: Ichneumonidae) Nelly, Novri; Habazar, Trimurti; Syahni, Rahmat; Buchori, Damayanti
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.996 KB) | DOI: 10.31258/jnat.13.3.250-255

Abstract

Temperature effect on development time of the preadult parasitoid Eriborus argenteopilosus Cameron(Hymenoptera: Ichneumonidae) were studied to know development time, degree days and survival rate.Crocidolomia pavonana (Fabricius)(Lepidoptera: Pyralidae) larvae was exposed to E. argenteopilosus female andreared at four different temperatures i.e 160C, 200C, 250C and 300C. Data were analyzed using anova and linearregression to calculate degree day. At 200C E. argenteopilosus showed the highest degree day and survival rate(18.67 %), while at 300C nothing adult parasitoid emergenced. Degree day to development time of parasitoid attemperature 200C i.e fase egg-adult: 300.05; egg-pupae 173.35; pupae-adult 171.
Inokulasi Fungi Mikoriza Arbuskula (FMA) Indigenus pada Bibit Jahe untuk Pengendalian Penyakit Layu Ralstonia solanacearum ras 4) Suharti, Netty; Habazar, Trimurti; Nasir, Nasril; Dachryanus, Dachryanus; Jamsari, Jamsari
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.568 KB) | DOI: 10.31258/jnat.14.1.61-67

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum race 4 is one of factors inhibiting ginger production. The study on ArbuscularMychorrizae Fungus (AMF) in greenhouse was found could reduce of bacteria wilt disease. Experiment design of research was randomizedblock design using 8 isolates. The results showed that inoculation of Arbuscular Mychorrhizae Fungus isolated from healthy gingerrhizosphere as biological diseases control agents could reduces disease severity. Four isolate could reduced disease severity up to 100% andsupport plant growth and production. As the result the seedling formation increased by 50–150%, plant height 36.92–87.56%, the numberof leaf 61.94–162.22% and ginger yield 190.62–400% respectively, compared to the control.
Perubahan Bentuk Planlet Pisang Raja Sereh Hasil Mutasi dengan Ethyl Methane Sulphonate (EMS) Secara In Vitro Yanti, Yulmira; Habazar, Trimurti; ', Mardinus; ', Mansyurdin
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.546 KB) | DOI: 10.31258/jnat.11.2.104-108

Abstract

The shoot of “rajasereh” banana was treated by 0,2% and 0,5% of ethyl methane sulfonate (EMS), for 2 and 4 hoursthourgh in vitro. The results showed that treatment of EMS mutagen would be changed morphologycal characterseither in planlet. In planlet stage was obtained four morphololgycal variations. One of the variant have characterizedthe colour yellowish; the leaves and stem are yellowish: the leaves were small and spiral. The value of coefficientof variant for morfphologycal characters of planlet “raja sereh” banana increased compare to the control. Theextreme value of coefficient of variant in planlet stage was found the times shoots is 84,31%, while control that is8,24%. EMS mutagen could caused several planlets shorter, total of leaves could of shoot are mostly
Senyawa Phenolik Akar Pisang CV. Kepok (Musa acuminata) yang Diinduksi dengan Fungi Mikoriza Arbuskular Indigenus PU10-Glomus sp 1 terhadap Penyakit Darah Bakteri Suswati, Suswati; Habazar, Trimurti; Husin, Eti Farda; Nasir, Nasril; Putra, Dedi Prima; Taylor, Peter
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.413 KB) | DOI: 10.31258/jnat.13.3.207-213

Abstract

Cooking banana (Musa acuminata) cv. Kepok is the most susceptible to Blood disease bacterium (BDB) infection.From previous study revealed the best isolate indigenous Arbuscular Mycorrhiza Fungi-Pasar Usang 10 (PU10-Glomus sp 1) could induce cv.Kepok resistance to BDB in green house and field experiment. The AMF could changethe phenolic compound in root plant. This objectives were to measure the root phenolic compound and bioassayto BDB. The 50 grams fresh inoculant PU10-Glomus sp 1 were applicated to banana root plants 60 days old with 6levels time course: 12; 24; 36; 48; 72; 92 hours and control (without PU10-Glomus sp 1). The root methanolicextraction followed to Echeverri et al., (2002) methode with vacuum concentration of the filtrate and partitioninginto ethyl acetate revealed the presence of an antibacterial compound as detected by TLC (Thin LayerChromatography), assay phenolic contained by Spectrofotometer UV-Vis 1700. PharmaSpec. Shimadzu andbioassay using BDB. Nine antibacterial compounds rose from root banana seedling colonized by PU10-Glomus sp1 in 12 hours after applicated (haa) ; 24; 36 and 48 haa. They were with Rf values of 0.16; 0.17; 0.19; 0.26; 0.32; 0.37;0.71; 0.80 and 0.83 on silica plates run in hexane:ethyl acetate (1:2 v/v) and control contained only 0.05 and 0.28.These compounds produced fluorescens which was bright yellow green spots and purple and have antimicbrobialproperties to BDB.
AKTIVITAS ENZIM PEROKSIDASE BAWANG MERAH YANG DIINTRODUKSI DENGAN BAKTERI ENDOFIT DAN TAHAN TERHADAP PENYAKIT HAWAR DAUN BAKTERI (XANTHOMONAS AXONOPODIS PV. ALLII) Resti, Zurai; Habazar, Trimurti; Putra, Deddi Prima; ., Nasrum
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.172 KB) | DOI: 10.23960/j.hptt.216131-137

Abstract

Peroxidase enzyme activity of the introduced shallots with endophytic bacteria and resistant to bacterial leaf blight (Xanthomonas axonopodis pv. allii). Bacterial leaf blight caused by Xanthomonas axonopodis pv. allii is an important disease in shallots. We have earned six isolates of endophytic bacteria, which have the ability to induce systemically resistance to shallots. One mechanism in induce resistance in plants is a change in the plant defense enzyme activity such as peroxidase. The purpose of this study was to calculate the peroxidase enzyme activity of shallots crop is being introduced with endophytic bacteria and is able to induce resistance to Xanthomonas axonopodis pv. allii. This research was conducted by introducing six isolates of endophytic bacteria on shallot bulbs and planted in greenhouse. Shallots crop that was 14 days old then inoculated with the bacterium Xanthomonas axonopodis pv. allii and incubated until symptoms appear. Peroxidase enzyme activity was calculated on the roots and leaves of shallots are 0, 1, 2, 4, 6, 8, 10, 15, and 30 days after inoculation (dai). The results showed an increase in the peroxidase enzyme activity of shallots crop is being introduced by endophytic bacteria compared to the control. Isolates ULG1E2 (Serratia marcescens PPM4) was isolate with the highest peroxidase enzyme activity both in the roots and leaves are 0,051 ìm/ ml.
FORMULASI PADAT RHIZOBAKTERIA INDIGENUS BACILLUS THURINGIENSIS TS2 DAN WAKTU PENYIMPANAN UNTUK MENGENDALIKAN PENYAKIT PUSTUL BAKTERI XANTHOMONAS AXONOPODIS PV. GLYCINES Yanti, Yulmira; Habazar, Trimurti; Resti, Zurai
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 1 (2017): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1732.39 KB) | DOI: 10.23960/j.hptt.1179-18

Abstract

Solid formulations of indigenous rhizobacteria Bacillus thuringiensis TS2 and storage time to control bacterial pustule disease Xanthomonas axonopodis pv. glycines. Bacterial pustule disease caused by Xanthomonas axonopodis pv. Glycines is a major constraint in soybean cultivation. Indigenous rhizobacteria Bacillus thuringiensis TS2 from soybean rhizosphere acquired from previous research is the best isolate which can control soybean bacterial pustule disease and increase growth rate of soybean. To increased its stability and interaction with soybean plants, Bacillus thuringiensis TS2 was urged to test furthermore especially its formulation with based formula tapioca powder, peat and bulk. The most effective storage time also need to test. Result showed that all rhizobacterial formula had ability to decrease incidence of bacterial pustule disease compared to control. Moreover, all the three formula could increase plant growth, total of leaves, total of branch and yields. Flowering time was also advanced by 1-8 days compared to control. Decreasing of disease rate and increasing of plant growth rate variated between different formulations.
CHARACTERIZATION OF ENDOPHYTIC BACILLUS ISOLATED FROM SHALLOT ROOT AS BIOCONTROL OF BACTERIAL LEAF BLIGHT DISEASE Resti, Zurai; Habazar, Trimurti; Putra, Deddi Prima; ., Nasrun
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 1 (2018): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.334 KB) | DOI: 10.23960/j.hptt.11831-38

Abstract

Characterization of Endophytic Bacillus Isolated from Shallot Root as Biocontrol of Bacterial Leaf Blight Disease. Endophytic Bacillus isolated from the roots of healthy shallots has potential as biological control agent of bacterial leaf blight (BLB) disease. Based on the in planta screening, four endophytic bacteria that capable of controlling BLB diseases from the group of Bacillus were obtained, such as Bacillus cereus P14, Bacillus cereus Se07, Bacillus sp. HI, and Bacillus sp. SJI. The aims of this study were to investigate the characteristics of endophytic Bacillus that capable of controlling BLB disease and to determine the characteristic that most responsible in the disease control. This study method was descriptive. The characteristics observed were production of antibiotics by paper disc method, production of salicylic acid by capillary electrophoresis, and root colonization by in planta method. Linear regression analysis was used to determine the characteristic most responsible in the disease control. The results showed that four endophytic Bacillus were capable of producing salicylic acid and colonizing the root, and three of them were also capable of producing antibiotic. Salicylic acid production varied ranging from 13.96 to 14.72 ppm mL-1. Three endophytic Bacillus were capable of producing antibiotic with inhibition zone of 16.25 to 20.25 mm. Endophytic Bacillus was able to colonize the shallot roots with a population of bacteria ranged from 3.20 × 105-6, 20 × 105 CFU g-1 root. Based on the correlation coefficient of linear regression analysis, the root colonization of endophytic bacteria played the role in decreasing BLB disease.
INTRODUKSI FORMULA FUNGI MIKORIZA ARBUSKULA DARI RIZOSFER PISANG PADA BIBIT PISANG UNTUK PENGENDALIAN PENYAKIT DARAH BAKTERI (RALSTONIA SOLANACEARUM PHYLOTYPE IV) Yefriwati, Yefriwati; Habazar, Trimurti; Husin, Eti Farda
JURNAL AGROTROPIKA Vol 16, No 1 (2011): Agrotropika Vol.16 No.1 2011
Publisher : JURNAL AGROTROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.012 KB)

Abstract

Blood disease bacteria (BDB) caused by Ralstonia solanacearum Phylotype IV (Pseudomonas solanacearum) is one of the most important diseases on banana. Using biological agents such as arbuscular mycorrhizal fungi (AMF) to control BDB is still not maximal result, based on that need to search a potensial indigeneous AMF specific location. The aim of this experiment were to study the stability of formulated AMF indigenous to control BDB and to increase growth of banana seedlings. This research was arranged by Factorial in Randomized Complete Design (RCD) on green house experiment with 5 replicate. The treatment consist of 2 factors : 1) enrichment of carrier of AMF, sand with rock phosphate (0, 10, 20 and 40 %). 2) incubation periode (0, 1 and 2 months). Banana seedlings were intruduced with formulated AMF at planting date. Two month old banana seedlings were inoculated with Ralstonia solanacearum Phylotype IV. The parameter were observed include: incubation periode, disease insidence, disease severity, discoloration of pseudostem, population density of Ralstonia solanacearum Phylotype IV, colonisation degree of AMF on banana root, spore density on rhizosphere, growth of banana seedlings.The results showed that all formulated AMF introduced on banana seedlings reduced BDB development and increase banana growth compare with control plants, especially the formulated AMF enriched with 30 % rock phosphate.Key Words: arbuscular mycorrhizal fungi, blood disease bacteria, biological controll agents, banana