Sri Hartati Hadinoto
Biochemistry Laboratory, Medical Faculty of Sebelas Maret University, Surakarta.

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Journal : Nexus Biomedika

Perbedaan Kadar Caffeic Acid Phenethyl Ester Pada Propolis di Pasaran Wilayah Surakarta Pramono, Devina Noviani; HP, Diding; Hadinoto, Sri Hartati
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Propolis is composed of several kinds of  biological and pharmacological components that can work as immunomodulator, anti-tumor, anti-inflammation and antioxidants. This is mainly due to its chemical composition, one of which is Caffeic Acid Phenethyl Ester (CAPE). The amount and composition of its chemical components are mainly affected by several factors, some of which are the type of plant vegetation, geographical position, solvent, bees, seasonal factor, and weather. This leads to the different composition of chemical component among different brands of propolis. The same phenomenon happens in Surakarta. This research aimed to reveal either or not the different of CAPE level exist among different brand of propolis found in Surakarta’s market. Methods: This research was an observational analysis using purposive sampling method. Samples were six different brands of propolis found in Surakarta’s market. The samples were divided into 6 groups which were P1, P2, P3, P4, P5 and P6. Each group was measured five times. The measurement of CAPE level of each sample was done using UV Vis spectrophotometer with wave length of 700 nm using Prussian Blue method. Data analysis was done using one way Anova statistical test with SPSS 17.00 for Windows program.   Results: The average level of CAPE found in propolis samples were P1 =11.531±0.078 µg/ml; P2 =5.438±0.106 µg/ml; P3 =12.541±0.052 µg/ml;  P4 =9.036±0.081 µg/ml; P5 =10.593±0.043 µg/ml; and P6 =10.366±0.133 µg/ml with p value is below 0.001 (p < 0.001). Conclusions: There was a difference of CAPE level among propolis found in the market area of Surakarta. Keywords: CAPE, Propolis