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Analog Rice Reduces Weight and Total Cholesterol Levels in Overweight and Hypercholesterolemic Rats Dewantari, Septiarina Putri; Hairrudin, Hairrudin; Sakinah, Elly Nurus
Jurnal Kedokteran Brawijaya Vol 31, No 1 (2020)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2020.031.01.2

Abstract

Overweight, whose prevalence is increasing, is caused by unbalanced consumption patterns such as high consumption of fat and can trigger hypercholesterolemia. Analog rice (AR), which has better nutritional content than ordinary rice, is thought to have beneficial effects on health. This study aimed to determine the potential of analog rice in reducing body weight and total cholesterol levels. A total of 24 male Wistar rats were divided into four groups, namely: I) control, II) ordinary rice treatment, III) analog rice 1 treatment (PBA1) and IV) analog rice 2 treatment (PBA2). The control group was given standard feed for 40 days ad libitum. Groups II, III, and IV were given a combination induction of high-fat diet (HFD) for 40 days ad libitum and intraperitoneal injection of streptozotocin (STZ) 35 mg/kg single dose on day 33. All groups were then given the treatment of diet for three weeks according to their respective groups, namely the standard, ordinary rice, analog rice 1, and analog rice 2 feed. Body weight and total cholesterol levels were measured in the pre-induction, post-induction/pre-treatment (pre-test), and post-treatment (post-test). The results of the study showed that the induction of the HFD-STZ combination carried out resulted in a significant increase in body weight and total cholesterol levels. Measurements made after dietary therapy showed that body weight and total cholesterol levels in the I and II groups did not change significantly, whereas those in the III and IV groups decreased significantly (p<0.05). This study concluded that the administration of analog rice could reduce body weight and total cholesterol levels in overweight and hypercholesterolemia rats. 
Aktifitas Fisik Berat Menyebabkan Degenerasi Sel Hepatosit melalui Mekanisme Stres Oksidatif hairrudin, Hairrudin; Helianti, Dina; Widiastuti, Yulestrina
Jurnal Kedokteran Brawijaya Vol 27, No 2 (2012)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2012.027.02.2

Abstract

Hepar mempunyai peranan yang penting dalam mempertahankan homeostasis metabolisme tubuh. Stres oksidatif akibat aktifitas fisik berat dapat meningkatkan produksi Reactive Oxygen Species (ROS) yang kemudian menyebabkan kondisi patologis pada sel hepatosit, ditandai dengan perubahan struktur dan fungsinya. Penelitian ini bertujuan untuk mengetahui dampak stres oksidatif akibat aktifitas fisik berat terhadap kerusakan sel hepatosit. Penelitian ini menggunakan 16 ekor tikus putih Wistar jantan yang berumur 3 bulan, dengan berat badan antara 150 s.d. 200 gram yang dibagi ke dalam kelompok kontrol dan perlakuan. Aktifitas fisik berat yang berupa renang dengan beban dan intensitas maksimal diberikan pada kelompok kontrol, dilakukan setiap pagi selama 30 hari. Penilaian stres oksidatif ditentukan melalui pengukuran kadar MDA serum menggunakan teknik Thiobarbituric Acid Reactive Substance (TBARS). Perubahan histopatologi sel hepatosit berupa degenerasi sel dinilai berdasarkan klasifikasi sistem skoring NAFLD dari the Pathology Committee of the NASH Clinical Research Network. Rerata kadar MDA pada kelompok kontrol sebesar 42,38 nmol/ml, sedangkan pada kelompok perlakuan sebesar 89,63 nmol/ml. Pada pengamatan histopatologi hepar menunjukkan adanya proses degenerasi sel. Dapat disimpulkan bahwa aktifitas fisik berat dapat menyebabkan degenerasi sel hepatosit melalui mekanisme stres oksidatif.Kata Kunci: Aktifitas fisik berat, degenerasi, sel hepatosit, stres oksidatif.
The Comparison of Bay Leaf and Celery Leaf Infusion Effect on Decreasing LDL Level in Dyslipidemic Wistar Rats Model Retnaninggalih, Anjani Putri; Efendi, Erfan; Hairrudin, Hairrudin
Journal of Agromedicine and Medical Sciences Vol 1, No 1 (2015)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

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Abstract

Coronary heart disease (CHD) is the cause of 7,3 million death globally in 2008. The main pathophysiology process that leads to CHD is atherosclerosis which is caused by high LDL cholesterol level. The aim of this study was to explore bay leaf and celery leaf infusion effect on reducing LDL cholesterol level in dyslipidemic rat model. The type of this study was true experimental research using wistar rat (Rattus novergicus) as the sample. The rats were induced by high fat diet and PTU for two weeks then each of the group were given either bay leaf infusion, celery leaf infusion, simvastatin (positive control), or aquadest (negative control) for a week. The measurement of LDL level used Friedewald formulation. Data was analyzed using paired sample t test and one way ANOVA. As the result there was a significance reduction on LDL level after treatment with bay leaf (p=0,013) and celery leaf infusion (p=0,035) but there was no significance difference between groups (p=0,293). It was concluded that bay leaf and celery leaf infusion could reduce the LDL level in dyslipidemic wistar rat but there was no difference on the capability of the two different leafs on reducing LDL level. Keywords: dyslipidemia, LDL, bay leaf, celery leaf, wistar rats  
Hubungan Paparan Pestisida dengan Kadar SGOT dan SGPT Petani di Desa Pakis Kabupaten Jember Zahrox, Iin Fatimatus; Hairrudin, Hairrudin; Sofiana, Kristianningrum Dian
Jurnal Kesehatan Lingkungan Indonesia Vol 20, No 1 (2021): April 2021
Publisher : Master Program of Environmental Health, Faculty of Public Health, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkli.20.1.47-52

Abstract

Latar belakang: Hepar merupakan salah satu organ yang rentan terhadap paparan pestisida. Akumulasi pestisida di dalam hepar tidak dapat diuraikan maupun diekskresikan. Penumpukan pestisida pada hepar memicu peningkatan jumlah radikal bebas dan menyebabkan gangguan permeabilitas membran sel hepatosit. Kondisi tersebut mengakibatkan kerusakan sel hepatosit dan meningkatkan kadar SGOT maupun SGPT. Desa Pakis merupakan salah satu desa penghasil padi terbesar di Kabupaten jember dengan mayoritas penduduk bekerja sebagai petani. Penelitian ini bertujuan untuk menganalisis hubungan paparan pestisida terhadap kadar SGOT dan SGPT pada petani di Desa Pakis Kabupaten Jember.Metode: Penelitian ini merupakan penelitian observasional analitik dengan desain penelitian cross sectional. Sampel pada penelitian ini adalah petani padi Desa Pakis yang menggunakan pestisida organofosfat atau karbamat Pengambilan sampel dilakukan menggunakan teknik non probability sampling dengan metode accidental sampling. Data diperoleh melalui wawancara dan pengukuran kadar asetilkolinesterase, SGOT dan SGPT menggunakan fotometer.Hasil: Sampel berjumlah 30 orang. Tingkat paparan pestisida yang tinggi ditunjukkan oleh kadar asetilkolinesterase <75% ditemukan pada 23,33% dari sampel. Sebagian besar berusia > 50 tahun dan telah bekerja lebih dari 10 tahun. Peningkatan kadar SGOT dan SGPT didapatkan masing-masing sebesar 33,33% dan 23,33%. Hasil penelitian menunjukkan bahwa tidak ada hubungan yang signifikan antara paparan pestisida dengan kadar SGOT (p = 0,320) dan SGPT (p = 604).Simpulan: Tidak ada hubungan antara paparan pestisida dengan kadar SGOT dan SGPT pada petani di Desa Pakis Kabupaten Jember. ABSTRACT Title: The Correlation of Pesticides Exposure with Farmer’s SGOT and SGPT level in Pakis Village, Jember RegencyBackground: The Liver is one of the organs that are vulnerable to pesticide exposure. Accumulated pesticides in the liver cannot be broken down or excreted. Pesticides that build up in the liver triggers an increase in the number of free radicals and cause an impaired hepatocyte membrane permeability. These conditions result in damage to hepatocyte cells and an increase in SGOT/SGPT levels. Pakis village is one of the biggest paddy producers in Kabupaten  Jember with the majority of its people works as farmers. This research aims to analyze the correlation of pesticide exposure and SGOT/SGPT levels among farmers in Pakis village, Jember regency. Method: This research is an analytic observational study using a cross-sectional research design. Samples were farmers who had been using organophosphate or carbamate pesticides. Sample collected using a nonprobability sampling technique with the accidental sampling method. Data obtained through interviews and measurements of acetylcholinesterase, SGOT, and SGPT levels using a photometer.Result: A number of 30 subjects participated. A high degree of pesticide exposure as indicated by acetylcholinesterase level of <75% was encountered in 23.33% of samples. Most samples were > 50 years old and had worked for more than 10 years.. Increased SGOT and SGPT levels were encountered in 33.33% and 23.33%, respectively. This research has shown that there was no significant correlation between pesticide exposure with SGOT (p=0,320) and SGPT levels (p=604).Conclusion: No significant correlations were found between pesticides exposure with SGOT and SGPT levels in farmers in Pakis village, Jember regency.
Pengaruh Stres Fisik terhadap Kadar Kreatinin Serum Tikus Wistar Jantan (Rattus norvegicus) (The Effect of Physical Stress on Serum Creatinine of Male Rattus norvegicus) Putu Ratih Pradnyani Dewi; Hairrudin Hairrudin; Rena Normasari
Pustaka Kesehatan Vol 4 No 2 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Physical stress is a condition that caused by severe physical activities which can increase free radicals production causing oxidative stress. Oxidative stress causing the organs failure include the kidney. It damage glomerulus causing decrease in GFR and tubular cell damage causing cumulation of creatinine and raise creatinine serum. This study investigated the difference of serum creatinine of male Rattus norvegicus with physical stress and without physical stress. Ten male Rattus norvegicus aged 2-3 months were divided into two groups : control and treatment group. Control group without physical stress but treatment group was given physical stress 30 minutes of swimming stress in the morning everyday for 10 days. The creatinine serum measured by Jaffe reaction. The normal range of serum creatinine for Rattus norvegicus was 0,578-1,128 mg/dl. The result of serum creatinine for control group was 0,52 mg/dl and treatment group was 0,62 mg/dl,both still in normal range. The analysis using Mann Whitney showed the significance of 0,23 (p>0,05). We can conclude that there was no difference of serum creatinine of male Rattus norvegicus with physical stress and without physical stress. Keywords: physical stress, oxidative stress, serum creatinine
Efektivitas Ekstrak Putri Malu (Mimosa pudica Linn.) sebagai Nefroprotektor pada Tikus Wistar yang Diinduksi Parasetamol Dosis Toksik Ari Setyo Rini; Hairrudin Hairrudin; Sugiyanta Sugiyanta
Pustaka Kesehatan Vol 1 No 1 (2013)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

The ethanolic extract of Mimosa pudica Linn. was evaluated for its nefroprotective and antioxidant activity against paracetamol induced kidney toxicity in wistar rats. Liver damage was induced by administration of 1.350 mg paracetamol/kg body weight. The ethanolic extract of Mimosa pudica Linn. (400mg/kg, 600mg/kg, and 800 mg/kg) was administered orally to the rats for 6 days. Curcuma® was given as positive control. All of the test drugs were administered orally by suspending in 1% of Na CMC solution. The nefroprotective activity was assessed using the levels of BUN and creatinine serum. The result revealed that all doses of the extracts showed effective activity in inhibiting the increase of BUN and creatinine serum and the doses of 800 mg/kg showed the biggest percent value (9,4% of BUN level and 12,19% of creatinine level). The positive control group could prevent the increase of BUN and creatinine serum better when compared with the group of Mimosa pudica Linn.The result of the present study indicated that the ethanolic extract of Mimosa pudica Linn. significantly prevent the increase of the level BUN and creatinine serum in wistar rats induced with paracetamol toxic dose.Keywords: Antioxidant, nefrotoxic, Mimosa pudica, paracetamol, BUN, creatinine.  
Efek kandungan serat beras analog terhadap ekspresi GLUT4 otot rangka tikus diabetes Darajat, Azka; Sakinah, Elly Nurus; Hairrudin, Hairrudin
Jurnal Gizi Klinik Indonesia Vol 16, No 1 (2019): Juli
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, FK-KMK UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1070.355 KB) | DOI: 10.22146/ijcn.31806

Abstract

Effect of analog rice’s fiber on skeletal muscles GLUT4 expression in diabetic rats Background: Disruption of glucose transportation in skeletal muscle through GLUT4 becomes a problem in diabetes. Analog rice that had been modified by adding dietary fiber could improve the expression of GLUT4.Objective: This study aims to know the effect of dietary fiber toward GLUT4 expression and to know the dietary fiber percentage in analog rice.Method: The research type is true experimental with post-test only group design. The samples consist of 24 male Wistar rats that are group into 4 groups (n=6 each group). Three groups were induced by giving a high-fat diet for 40 days and streptozotocin (STZ) 35 mg/kg BW was given at 33th day and one group was not induced. After the blood glucose level exceeded 135 mg/dl, the treatment was given. After 3 weeks, the rats were terminated and quadriceps femoris muscle tissue was taken for immunohistochemistry examination using rat GLUT4 polyclonal antibody. GLUT4 expression was quantified using an immunoreactive score (IRS-GLUT4). The data were analyzed using the Kruskal-Wallis test and Spearman test.Results: Statistical analyses showed that there were significant differences between groups with a moderate positive correlation (correlation coefficient=0,651; p=0,003).Conclusion: Dietary fiber in analog rice could improve skeletal muscle GLUT4 expression in Wistar rat diabetic model.
‘Propolis’ Protective Effect to Prevent Oxidative Stress Caused by Strenous Physical Activity (Swimming Stress) Hairrudin Hairrudin
Jurnal ILMU DASAR Vol 10 No 2 (2009)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.464 KB)

Abstract

Strenous physical activity may give not only a possitive impact but also the negative one. One of the factors that produce negative impact is the increasing formation of oxydant that may cause tissues’ damages. In this kind of circumstances, the oxydant could make damages to the various components in the body, it calls oxydative stress. The effect of the oxydant to the unsaturated fatty acid cause a chain reaction that will break the fatty acid to become different kinds of substances, such as malondialdehid (MDA). The MDA produced then released into the circulatory system, made the serum level of MDA increasing. The influence of propolis as antioxidant may be prevent this problem. This study is a laboratory experiment using posttest-only control group design.  The samples of the experiment are 3-month male white rat with average weight between 180-220 grams. These samples are divided to three groups: (1) control group (2) exposure group that have to do strenous activity (30 days swimming stress) and (3) exposure group that have to do strenous activity (30 days swimming stress) and given propolis, each group consists of eight rats. The result of the study showed a higher average serum MDA level in exposure group (89.63 nmol/mL) than the control group (42.38 nmol/ml) significantly (p = 0.000). The influence of propolis could prevent oxidative stress wich was indicated by lower average serum MDA level (32.35 nmol/ml) significanly (p = 0.000).
Hepatoprotector Effect of Coconut Water (Cocos nucifera L.) and Folic Acid to the Liver Histopathological Desccription of Pregnant Wistar Female Rats (Rattus norvegicus) Induced by Carbamate Muhammad Rosyid Ridho; Aris Prasetyo; Hairrudin Hairrudin
Journal of Agromedicine and Medical Sciences Vol 6 No 1 (2020)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v6i1.10758

Abstract

Abstract Carbamate is an insecticide with a working mechanism that inhibits the enzyme acetylcholineestrase (AChE). Obstacles to AChE will cause the formation of excessive free radicals in the body causing oxidative stress and causing lipid peroxidation in body cells, including hepatocyte cells in the liver. Pregnant women have a change in detoxification activity in the liver due to exposure to xenobiotic substances during pregnancy causing a decrease in cytochrome P450 1A2 (CYP1A2) which will cause an increase in free radicals and can damage liver cells. Coconut water contains antioxidants that can neutralize free radicals in the body. While folic acid can encourage improvement in morphology of liver cells. The purpose of this study was to determine the hepatoprotector effect of coconut water and folic acid on the histopathology of the liver of pregnant female wistar rats induced by carbamate. The design of this study is true experimental with a post test only control group design. The sampling method used is simple random sampling. The number of samples in this study were 28 rats divided into 4 groups K (aquades), P1 (carbamate), P2 (carbamate and coconut water), and P3 (carbamate and folic acid). At the end of the study rat liver was taken to then become histological preparations. The One Way ANOVA test results showed a significant difference between groups compared (p <0.05). Post hoc LSD test results showed that group P1 had damaged liver histopathology, there were significant differences with group K and P3 (p <0.05), but there were no significant differences when compared with group P2 (p = 0.826). Meanwhile, the P3 group had significant differences when compared with all groups (p <0.05). The conclusion of this study is that giving coconut water cannot prevent liver damage due to carbamate induction, while folic acid has been shown to prevent liver damage due to carbamate induction, from liver histopathology. Keywords: carbamate, coconut water, folic acid, liver histopathology
Efek Preventif Ekstrak Etanol Kopi Robusta (Coffea canephora) terhadap Peningkatan Kadar Kolesterol Total Tikus Jantan Galur Wistar yang Diinduksi Kuning Telur Yan Agus Achtiar; Hairrudin Hairrudin; Kristianningrum Dian Sofiana
Pustaka Kesehatan Vol 2 No 3 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Kopi robusta mengandung asam klorogenat (CGA) sekitar 7-14%. Asam klorogenat diduga dapat mencegah penyerapan kolesterol diusus. Penelitian ini bertujuan untuk membuktikan efek preventif ekstrak etanol kopi robusta (Coffea canephora) terhadap peningkatan kadar kolesterol total tikus. Penelitian ini adalah penelitian eksperimental laboratorium pre-post-test dengan kelompok kontrol. Dua puluh emapt ekor tikus wistar jantan usia 3-4 bulan, dibagi menjadi kelompok perlakuan K1 diinduksi kuning telur, kelompok K2 diinduksi kuning telur dan ekstrak kopi dosis terapi (0,5 mL / ekor), kelompok K3 diinduksi kuning telur dan simvastatin, kelompok K4 diinduksi kuning telur dan ekstrak kopi dosis tinggi (1 mL / ekor) selama 35 hari. Pada awal dan akhir perlakuan diambil serum darah untuk mengetahui kadar kolesterol total darah menggunakan menggunakan metode CHOD-PAP. Dalam waktu 35 hari, pemberian ekstrak kopi robusta tidak mencegah kenaikan kadar kolesterol total. Sehingga dapat disimpulkan bahwa tidak ada efek preventif ekstrak etanol kopi robusta (Coffea canephora) terhadap peningkatan kadar kolesterol total tikus wistar jantan yang diinduksi kuning telur.   Kata kunci: Hiperkolesterolemia, asam klorogenat, kolesterol total serum