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All Journal Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Indonesian Journal of Biotechnology Journal of Agromedicine and Medical Sciences Majalah Kedokteran Sriwijaya Syntax Literate : Jurnal Ilmiah Indonesia SRIWIJAYA JOURNAL OF MEDICINE Jurnal Entomologi Indonesia Bioscientia Medicina : Journal of Biomedicine and Translational Research Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Bioscientia Medicina : Journal of Biomedicine and Translational Research Arkus Conferences of Medical Sciences Dies Natalis Jurnal Ilmu Kesehatan Masyarakat
Dwi Handayani
Department Of Parasitology, Faculty Of Medicine, Universitas Sriwijaya
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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PREVALENSI MALARIA PADA DAERAH ENDEMIS ORANG RIMBA PROVINSI JAMBI MENGGUNAKAN PEMERIKSAAN MIKROSKOPIS Ade Suryaman; Chairil Anwar; Dwi Handayani; Sulfa Esi Warni; Irsan Saleh; Dalillah Dalillah; Gita Dwi Prasasty
Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Vol 4 No 1 (2020): Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health)
Publisher : Poltekkes Kemenkes Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35910/jbkm.v4i1.265

Abstract

Background: Malaria remain a world health problem with the highest number of case are in tropic and subtropic country such as Indonesia. Orang Rimba is a native tribe living secluded deep in the Taman Nasional Bukit Duabelas Forest and in the northern area were living in the Taman Nasional Bukit Tigapuluh. For demography, tteh were unregistered due to the difficulties of the survey, since they were living nomaden. Lack of transportation, living deep in the forest, low education level, bad living environment and low social economic level affect the risk of Malaria case number. This research amied to identifiy the prevalence of Malaria on endemic location of orang rimba in jambi province using microscopic examination. Methods: This was a descriptive observational study. The population in this study were all communities of Anak Rimba. A total of 94 Anak Rimba were recruited for the study.Malaria status were checked using gold standar which is Microscopical examination of the blood smear. Results: The results showed that 4,95% of the respondents were positive Malaria with plasmodium vivax (3,96%) and 0,99% special falciparum Conclusion: There were high prevalence of Malaria on Anak Rimba at Sungaiterap Desa Jelutih, Kec. Batin XXIV, Kabupaten Batangahari, Provinsi Jambi
Performance of CO1 and ITS2 nested PCR in molecular identification of ordinary scabies (Sarcoptes scabiei var. hominis) Gita Dwi Prasasty; Miftahurrizqiyah Miftahurrizqiyah; Chairil Anwar; Dwi Handayani; Dalilah Dalilah; Ahmad Ghiffari; Inda Astri Aryani; Nunuk Dyah Retno Lastuti; Afiat Berbudi
Indonesian Journal of Biotechnology Vol 26, No 4 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.64472

Abstract

Scabies is a global disease with a high prevalence, causing morbidity and even mortality, especially in poor and developing countries. However, it is often misdiagnosed due to varied and unspecified lesions. The gold standard technique for diagnosis is a microscopic examination, which requires experienced experts in finding mites, mainly in ordinary scabies. CO1 and ITS2 genes have been widely used in molecular identification to detect Sarcoptes scabiei and its variants. This study aimed to determine and compare the sensitivity and specificity of CO1 and ITS2 S. scabiei genes to the microscopic examination of scabies skin scrapings. The skin scrapings of 52 subjects with scabies diagnosed by anamnesis, physical examination, and dermoscopic examination were examined under a microscope and analyzed by nested PCR. The diagnostic test result showed that the sensitivity of nested PCR of both CO1 and ITS2 genes to micro‐ scope examination was 100%. However, the specificity of both CO1 and ITS2 nested PCR was poor (24% and 0%). Hence, CO1 and ITS2 nested PCR could be more suitable for screening ordinary scabies in humans than the microscopic examination.
KEPADATAN VEKTOR DAN STATUS RESISTENSI LARVA Aedes aegypti DI DESA PANCUR PUNGAH KECAMATAN MUARA DUA KABUPATEN OKUS TAHUN 2019 Dwi Handayani; Fadjar Siddiq Hidayatullah; Chairil Anwar; Sulfa Esi Warni; Dalilah Dalilah; Lasbudi P Ambarita; Gita Dwi Prasasty
JURNAL KEDOKTERAN DAN KESEHATAN Vol 7, No 2 (2020)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.267 KB) | DOI: 10.32539/JKK.V7I2.9710

Abstract

Keberhasilan pengendalian penyakit demam berdarah dengue tergantung dari status kerentanan vektor terhadap insektisida yang digunakan. Sampai saat ini dikenal dua vektor DBD yaitu Aedes aegypti sebagai vektor utama dan Aedes albopictus sebagai vektor sekunder. Tujuan penelitian ini untuk mengetahui kepadatan vektor Aedes aegypti dan status resistensinya terhadap larvasida Temephos di Desa Pancur Pungah Kabupaten Muara Dua Kabupaten Ogan Komering Ulu Selatan. Penelitian ini merupakan penelitian deskriptif observasional. Sampel pada penelitian ini adalah 100 buah rumah, tempat penampungan air, dan larva yang ditemukan di dalam tempat penampungan air. Tempat penampungan air dan larva didalamnya diidentifikasi kemudian dilakukan penghitungan dan uji larvasida dilakukan dengan menggunakan Temephos 0,02 ppm sebanyak 5 kali pengulangan. Hasil penelitian didapatkan sebanyak 389 tempat penampungan air dan 55 diantaranya terdapat larva. Hasil perhitungan menunjukkan HI: 35, CI: 14,1 dan BI: 55 dan uji larvasida menunjukkan semua larva yang dipaparkan terhadap Temephos 0,02 ppm mengalami kematian. Dari perhitungan tersebut didapatkan kepadatan vektor berada pada intensitas sedang dan status kerentanannya masih sensitive atau susceptible terhadap Temephos. Hasil penelitian ini diharapkan dapat digunakan sebagai bahan evaluasi bagi kebijakan pengendalian vektor DBD setempat.
KERAGAMAN SPESIES NYAMUK DI DUSUN SUKOHARJO, DESA BAYUNG LENCIR, KABUPATEN MUSI BANYUASIN Dalilah Dalilah; Faiqah Arina Dalilah; Gita Dwi Prasasty; Dwi Handayani; Susilawati Susilawati; Rahman Irpan Pahlepi
JURNAL KEDOKTERAN DAN KESEHATAN Vol 9, No 1 (2022)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/JKK.V9I1.16539

Abstract

Nyamuk adalah vektor penyakit yang menularkan patogen melalui gigitannya pada manusia dan binatang. Beberapa jenis penyakit tular vektor di Indonesia yang disebabkan karena gigitan nyamuk seperti Demam Berdarah Dengue (DBD), Malaria, Filariasis dan Chikungunya. Nyamuk vektor penyakit didominasi dari genus Anopheles, Aedes, Armigeres, Culex  dan Mansonia. Peran nyamuk Aedes salah satunya yaitu sebagai vektor penyakit DBD, nyamuk Anopheles merupakan vektor penyakit malaria dan filariasis dan genus lainnya yakni Armigeres dan Culex dapat menularkan patogen filariasis dan Japanese encephalitis. Kabupaten Musi Banyuasin termasuk dalam kategori endemik rendah malaria, selain itu daerah ini juga merupakan daerah endemik Demam Berdarah Dengue. Keberadaan keragaman nyamuk dapat berpotensi penularan penyakit lainnya di kabupaten ini. Penelitian ini dilakukan untuk mengidentifikasi karakteristik keragamanan nyamuk yang berpotensi menjadi vektor penyakit di Dusun Sukoharjo, Desa Bayung Lencir, Kabupaten Musi Banyuasin. Penelitian ini adalah penelitian deskriptif observasional yakni survei lapangan. Nyamuk di Dusun Sukoharjo ditangkap pada bulan Agustus  2021 dan di identifikasi pada Laboratorium Entomologi Balai Litbangkes Baturaja. Setiap nyamuk akan diklasifikasikan ke genus dan spesiesnya berdasarkan karakteristik morfologi pada kunci bergambar. Ditemukan empat genus nyamuk yaitu Aedes, Armigeres, Culex, dan Anopheles dengan proporsi berturut-turut 2,6%, 5,1%, 88,5%, dan 3,8%. Sebelas spesies nyamuk ditemukan terdiri dari Aedes lineatopenne, Aedes aegypti ,Armigeres subalbatus, Armigeres kesselli, Culex gelidus, Culex nigropunctatus, Culex quinquefasciatus, Culex tritaeniorhynchus, Culex vishnui, Anopheles kochi, dan Anopheles vagus. Proporsi nyamuk paling banyak ditemukan yakni nyamuk Culex quinquefasciatus. Perlu menjadi kewaspadaan karena nyamuk Culex merupakan potensial vektor untuk penyakit filariasis dan Japanese Enchepalitis. 
Association of Smoking Habit With Nasopharyngeal Carcinoma Stage in ENT-HN Policlinic RSUP DR. Mohammad Hoesin Palembang Kemas Muhammad Alwan Dwiputra; Dwi Handayani; Denny Satria Utama
Majalah Kedokteran Sriwijaya Vol 51, No 2 (2019): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/mks.v51i2.8537

Abstract

Karsinoma nasofaring (KNF) adalah tumor ganas yang berada dibagian mukosa rongga nasofaring yang disebabkan oleh banyak faktor, salah satu faktornya adalah merokok. Kebiasaan merokok meningkatkan kepekaan terhadap terjadinya KNF. Merokok menyebabkan terjadinya mutasi gen p53, gen p53 memegang peranan terhadap kerusakan DNA sel dengan jalan menghambat progresi sel. Hilangnya fungsi gen p53 menyebabkan hilangnya kontrol pada siklus sel, sehingga terjadi proliferasi sel-sel sehingga besar peluangnya untuk menjadi sel kanker. Penelitian ini bertujuan untuk menganalisis hubungan kebiasaan merokok dengan stadium karsinoma nasofaring.Penelitian ini adalah studi observasional analitik dengan rancangan cross sectional. Sampel adalah pasien poliklinik THT-KL dan rekam medik pasien karsinoma nasofaring di RSUP Dr. Mohammad Hoesin Palembang bulan Januari 2017-Desember 2018.Sampel penelitian ini berjumlah 70 kasus.Hasil penelitian ini akan disajikan dalam bentuk tabel dan narasi.Dari 70 kasus pasien KNF didapatkan semua penderita adalah laki-laki (100%), kelompok usia ?50 tahun (52,9%), pekerjaan non PNS (90%), alamat diluar Palembang (82,9%), keluhan utama benjolan pada leher (42,9%) dan stadium 4 (81,4%). Hasil uji Fisher’s Exact Test tidak terdapat hubungan yang bermakna antara jenis rokokdengan stadium KNFdinyatakan oleh p value=0,067, terdapat hubungan yang bermakna antara jumlah konsumsi rokokdengan stadium KNFdinyatakan oleh p value=0,042 dan tidak terdapat hubungan yang bermakna antara usia mulai merokokdengan stadium KNFdinyatakan oleh p value=1,000.Hasil uji korelasi pada pasien KNFmenunjukkan bahwa jumlah rokok (p= 0,043)merupakan faktor determinan terjadinya stadium KNFdanjenis rokok (p= 0,198) bukan merupakan faktor determinan terjadinya stadium KNF. Terdapat hubungan yang bermakna antara kebiasaan merokok dengan stadium KNF.
Activity of Cytochrome P450 Monooxygenase (CYPs) Metabolic Enzymes as Markers of Insecticide Resistance in Anopheles vagus Muara Enim Mosquitoes, Indonesia Dalilah; Muhaimin Ramdja; Dwi Handayani; Lasbudi P Ambarita; R. Irpan Pahlepi; Ahmad Ghiffari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 4 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i4.222

Abstract

The use of synthetic pyrethroids in insecticide-treated bed nets (LLINs) and spray form (IRS) has been carried out since 2012 in Muara Emil Village and since 2016 in all villages in Tanjung Agung District, Muara Enim Regency. Biochemical resistance can occur to the enzymes that play a role in detoxifying the insecticide. This study aims to identify whether there is an increase in the levels of the enzyme Cytochrome P450 monooxygenase (CYPs) through the enzyme biochemical test on the malaria vector mosquito Anopheles vagus originating from Pagar Dewa Village and Muara Emil Village, Muara Enim Regency. The sample of female Anopheles mosquitoes was not full. Blood was taken at night using the resting collection method. Mosquitoes that have been identified as Anopheles vagus species are used to check CYPs enzyme levels using the ELISA method. The results of the biochemical test showed that enzyme levels increased from the Muara emil and Pagar Dewa village mosquitoes with a percentage of 71% and 61.53% (with cut off point OD> 0.165). The value of CYPs enzyme levels for mosquitoes from Muara Emil village was higher than that of the mosquitoes from Pagar Dewa Village (mean 0.005027 ± 0.007). The increased activity of the CYPs enzyme plays a role in the detoxification of synthetic pyrethroid insecticides that can cause resistance. The high levels of enzymes from the villages of Muara Emil and Desa Farah Dewa are in line with intensive insecticide exposure in eradicating malaria vectors in the highest malaria transmission areas in Muara Enim Regency.
Philogenetic Analysis of S. Scabiei Var. Hominis, Var. Cuniculi, and Var. Marmoota Gita Dwi Prasasty; Miftahurrizqiyah; Dalilah; Dwi Handayani; Tia Sabrina; Ahmad Ghiffari; Inda Astri Aryani
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 10 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i10.352

Abstract

Introduction: Based on data from the 2017 Global Burden of Disease, it is known that Indonesia is the country with the highest number of scabies incidences out of 195 countries in the world. In addition to humans, scabies is also an infectious disease in mammals with a prevalence of 300 million infestations every year, even in animals the mortality rate from scabies is very high. Until now, it is still unclear whether there is a cross-infestation between human and animal scabies. Morphologically, S. scabiei between variants cannot be distinguished, while research on genetic variation is still not much done. Therefore, it is necessary to conduct studies to enrich genetic information and phylogenetic analysis. Methods: The samples tested were positive PCR results for ITS 2 and CO 1 genes, each on five amplicons, namely three S. scabiei var. hominis, one var. cuniculi, and one var. marmoota. Phylogenetics were arranged based on the closeness of the base arrangement in variants and geographically available in NCBI. Results: Of the five sample isolates studied, the ITS 2 gene amplicon is located in the 417bp band and the phylogenetic analysis of its sequencing has two branches that are unable to distinguish var. hominis and var. animalia. The CO1 gene has electrophoretic results at 317 bp as well as phylogenetic analysis results that specifically divide branches between var. hominis and var. animalia. Conclusion: S. scabiei var. hominis and var. marmoota, and var. cuniculi are distinct species and are host monospecific.
Contamination of Soil Transmitted Helminths (STH) Eggs in Raw Vegetables at Street Food Stalls and Restaurant in Lorok Pakjo Village, Palembang Leo Medianto Faziqin; Dalilah; Dwi Handayani; Chairil Anwar; Susilawati
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 6 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i6.397

Abstract

Background: Infection Soil Transmitted Helminths (STH) is a disease that is a problem in the world, especially in areas with a tropical climate like Indonesia. This infection can be transmitted through consumption of raw vegetables contaminated with STH. This study aims to determine whether there is contamination of STH eggs in raw vegetables at street food stalls and restaurants in Lorok Pakjo Village, Palembang. Methods: This research is a descriptive observational study. Samples in the form of fresh vegetables were collected from 18 street stalls and 14 restaurants in Lorok Pakjo Village, Palembang. Samples were examined using the sedimentation method and observed using a microscope. Results: The results obtained 80 samples consisting of 30 cabbage, 18 basil, 30 cucumber, 1 lettuce, and 1 eggplant. Of the 80 samples, there were 20 (25%) positive samples contaminated with STH eggs consisting of 8 (10%) cabbage samples, 8 (10%) basil samples, 4 (5%) cucumber samples, and no STH contamination was found in lettuce. and eggplant. Of the 20 positive samples of STH contamination, 12 samples came from street vendors consisting of 5 (10.6%) cabbage samples, 6 (12.8%) basil samples, and 1 (2.1%) cucumber samples. While the other 8 samples came from restaurants consisting of 3 (9.1%) cabbage samples, 2 (6%) basil samples, and 3 (9.1%) cucumber samples. Found only eggs of Ascaris lumbricoides infertile decorticate in all samples consisting of 9 eggs on cabbage, 16 eggs on basil, and five eggs on cucumber. Conclusion: There is contamination of eggs Soil Transmitted Helminths in raw vegetables in street food stalls and restaurants in Lorok Pakjo Village, Palembang.
Efforts to Prevent Variations of Skin Diseases in Islamic Boarding Schools through Personal Hygiene and Sanitation Education Gita Dwi Prasasty; Dwi Handayani; Dalilah
Arkus Vol. 5 No. 1 (2019): ARKUS
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/arkus.v5i1.75

Abstract

The condition of residents and environment of the boarding school which is not good has formed several variation of skin diseases that are repeated and almost similiar in every Islamic boarding school in Indonesia. This is a challenge for the Islamic boarding school community, health workers and academics to prevent, at least reduce the prevalence of these diseases. This activity was carried out in two stages, examination and education based on the results of the skin disease pattern that has been obtained from previous examinations. Education was performed online about diseases variation and how to cope them from the aspects of personal hygiene and environmental sanitation at the Pondok Pesantren Kampung Tauhiid Sriwijaya. This activity aimed to provide an coping of the variation of skin diseases that will be encountered by students and an accurate understanding of its prevention through personal hygiene and environmental sanitation.
Investigasi resistensi Anopheles sp. terhadap insektisida piretroid dan kemungkinan terjadinya mutasi gen voltage gated sodium channel (VGSC) Didid Haryanto; Dalilah Dalilah; Chairil Anwar; Gita Dwi Prasasti; Dwi Handayani; Ahmad Ghiffari
Jurnal Entomologi Indonesia Vol 15 No 3 (2018): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.15.3.134

Abstract

Extensive and continuous use of pyrethroid insecticides to prevent the transmission of malaria can lead to mutations in the voltage gate sodium channel gene (VGSC) in mosquito vectors. This gene mutation associated with insensitivity pyrethroid is known as knockdown resistance (kdr). The emergence of resistance to pyrethroid insecticides in vector mosquitoes can be a barrier to the successful termination of malaria transmission. Therefore, detection of mutation is necessary to prevent the resistency from build up. The aim of the study was to determine the resistance status of pyrethroid insecticides and identify mutations in the VGSC gene codon 1014 marker for resistance to Anopheles sp. which is a malaria vector in South Sumatra Province. Samples were taken from three districts in South Sumatra: Muara Enim, OKU, and Lahat. The susceptibility enzymatic test of pyrethroid insecticide (permethrin 0.75%) was preliminarily carried out according to WHO 2016 standard on Anopheles vagus Dönitz species. Identification of VGSC gene mutation was performed on all An. vagus that were previously tested for susceptibility and on An. barbirostris van der Wulp species using seminested-PCR and followed by sequencing. The result showed that samples from Muara Enim regency had resistance, whereas in Lahat and OKU regencies were still susceptible to permethrin. From the sequence analysis it is shown that there are no change in DNA kdr bases in VGSC gene insecticide target sites from all regencies. In conclusion, based on molecular studies, there were no pyrethroid insecticide resistance in South Sumatra Province.
Co-Authors Ade Suryaman Ade Suryaman Afiat Berbudi Ahmad Ghiffari Ahmad Giffari Anwar, Chairil Chairil Anwar Chairil Anwar Chairil Anwar Chairil Anwar Charil Anwar Dalilah Dalilah Dalilah Dalilah Dalilah Dalilah Dalilah Dalillah Dalillah Dalillah Dalillah Denny Satria Utama Desi Oktariana Desi Oktariana Desi Oktariana Didid Haryanto Evi Lusiana Fadjar Siddiq Hidayatullah Fatmawati Firmansyah Basir Gita Dwi Prasasti Gita Dwi Prasasty Gita Dwi Prasasty Gita Dwi Prasasty Gita Dwi Prasasty Gita Dwi Prasasty Gita Dwi Prasasty Hadrians Kesuma Putra Hartati Hurin ‘Afina Gnd Husna Syafira Muthmainnah Inda Astri Aryani Irsan Saleh Kamaluddin Kamaluddin Kemas Muhammad Alwan Dwiputra Lasbudi P Ambarita Lasbudi P Ambarita Leo Medianto Faziqin Mentari Permana Putri Miftahurrizqiyah Miftahurrizqiyah Miftahurrizqiyah Msy Rulan Adnindya Muhaimin Ramdja Muhammad Irsan Saleh Nia Savitri Tamzil Nia Savitri Tamzil Nunuk Dyah Retno Lastuti R. Irpan Pahlepi Rahman Irpan Pahlepi Rifka Purnama Sari Soilia Fertilita Sri Wiyani Sulfa Esi Warni Sulfa Esi Warni Sulfa Esi Warni Susilawati Susilawati Tia Sabrina Tia Sabrina Venny Larasati Zen Hafy