. Hasbi
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Effect of Separation Medium, Addition of Coffee Extract Before Sexing X and Y Sperm Chromosome and Storage Period on Quality of Fresh Semen of Ettawa Cross Goat . Hasbi; H. Sonjaya; S. Gustina
Jurnal Ilmu dan Teknologi Peternakan Vol. 1 No. 2 (2011)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.712 KB) | DOI: 10.20956/jitp.v1i2.670

Abstract

One problem in the application of biotechnology in spermatozoa sexing of X and Y sperm chromosome is a decrease in motility and percentage of live spermatozoa. The decrease could be caused by metabolic process, therefore spare energy obtained from separation medium is decreasing resulting in decrease in motility and percentage of live spermatozoa.  Research was carried out to evaluate the effects of separation medium, addition of coffee extract before sexing of X and Y sperm chromosome, and storage period on the quality of goat fresh semen.  This experiment was performed according to completely randomized design in factorial pattern (2 x 2 x 6). The first factor was the separation medium (A1: 10% and A2: 30%), the second factor was the coffee extract addition (B1: 0 mM and B2: 3 mM), and the third factor was the period of storage(D1: 0 hour, D2: 2 hours, D3: 4 hours, D4: 6 hours, D5: 8 hours and D6: 10 hours). The result showed that the motility of spermatozoa Y in 30% separation medium was higher (P<0.01) than that of spermatozoa X in 10% separation medium and it was higher (P<0.05) with addition of coffee extract compared with no coffee extract addition.  Viability of spermatozoa Y was higher (P<0.05) compared with spermatozoa X and addition of coffee extract not significantly different (P>0.05) compared with no coffee extract addition. The motility and viability of spermatozoa decreased during 10 hours storage. It was concluded that the motility and viability of spermatozoa Y was higher than spermatozoa X. Coffee extract can prevent a decrease of spermatozoa motility during storage. The motility of spermatozoa and the percentage of live spermatozoa decreased during storage.
THE INCIDENCE OF REPRODUCTIVE DISORDERS IN A DAIRY HERD: A CASE STUDY IN SINJAI REGENCY M. Yusuf; L. Rahim; . Hasbi; N. Aliah
Jurnal Ilmu dan Teknologi Peternakan Vol. 2 No. 1 (2012)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.201 KB) | DOI: 10.20956/jitp.v2i1.699

Abstract

The objective of this study was to ascertain the incidence of reproductive disorders in a dairy herd. A total of 44 Holstein Friesian cows in a dairy herd were enrolled in the present study. Trans-rectal palpation of the uterine was performed to determine the consistency of uterine including contraction, elasticity, tonicity, symmetry of uterine horns, and the presence of any fluid in the uterus followed by the presence of any palpable ovarian structures. Cows that did not become pregnant during clinical examination were examined vaginoscopically. Data obtained in the present study were analyzed descriptively based on percentages and means. T-test was used to analyze the interval from calving to first artificial insemination (AI) between cyclic cows and cows with reproductive disorders. The results of this study showed that incidence of reproductive disorders was very high; 38.6%. Inactive ovaries, cyst, and endometritis were the most reproductive disorders suffering the dairy cows. These reproductive disorders subsequently reduced reproductive performance by prolonged interval from calving to first AI as well as interval from calving to pregnancy. Lower BCS of the cows were invented as the most prominent causes of this issue.