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The Number of Macrophages and Heterophils on Chick Embryo Chorioallantoic Membrane After Gynura procumbens (Lour) Merr Extract Treatment and bFgF Induction Hamid, Iwan Sahrial; P, Yuseni Kusuma; Bijanti, Retno; Aksono, E Bimo
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Antiangiogenesis (inhibition of new blood vessels formation) has become a strategy to inhibit cancer development. The aim of this experiment was to investigate antiangiogenic effect  of  Gynura  procumbens  (Lour)  Merr  focusing  on  the  decreasing  of  the  number  of macrophages  and  heterophils  on  chick  embryo  chorioallantoic  membrane.  Nine-days-aged-eggs  were  divided  into  six  groups  (eight  eggs  each  group).  Group  I  (positive  control)  eggs were  induced  with  bFGF+Tris  HCl.  Group  II  (negative  control)  eggs  were  treated  with DMSO+Tris  HCl.  Group  III  (treatment  I)  eggs  were  induced  with  60  ng  bFGF  and  treated with  ethanolic  extract  of  G.  procumbens  leaves  with  the  dose  of  60  µg.  The  following treatment  groups,  i.e.  group  IV  (treatment  II),  group  V  (treatment  III),  and  group  VI (treatment IV) were treated with increasing dose of extract, starting from 75 µg, 90 µg, and the last was 110 µg. Eggs were incubated until they reach the age of twelve days to observe macrophages, while  to observe heterophils, eggs were incubated until  the  age of seventeen days. Based on haematoxylin-eosin staining, macrophages in the treatment groups were less than the control positive group (bFGF+Tris HCl), but based on giemsa staining, the effect of Gynura procumbens  in decreasing the number of heterophils could not  be observed  because some blood smears. These analysis suggest that  the  ethanolic extract of  Gynura procumbens leaves can perform as antiangiogenic agent decreasing the number of macrophages.Keywords: antiangiogenic, macrophages, heterophils, Gynura procumbens
Efektivitas Enhancer Natrium Lauril Sulfat Dalam Patch Topikal Antiinflamasi Ekstrak Etanol Kencur (Kaemferia Galanga L.) Terhadap Jumlah Neutrofil dan Makrofag Pada Mencit Fatmawati, Desy; Dermayati, Cyntia Zain; Hamid, Iwan Sahrial; Hendriati, Lucia
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 8, No 2 (2016)
Publisher : Indonesian Research Gateway

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Abstract

Ethanol extract Kencur (Kaempferia galanga L.) has been proven to have antiinflammatory effect as well as to decrease the side effect of NSAIDs drug use, to develop the formulation of ethanol extract of kencur, then made in the dosage form of topical patch. Topical patch preparations are expected to avoid effects on the gastrointestinal tract, first- pass metabolism, and facilitate the use of the patient thereby increasing patient compliance. In the patch formulation, the most important things to note is the penetration enhancer/ enhancer, and enhancer used in this study is sodium lauryl sulphate. This study aims to determine the effectiveness of sodium lauryl sulphate in the preparation of a topical patch of kencur extract as anti inflammatory on the number of neutrophils in the blood. The research method uses canrrageenan as an inflammation inductor and neutrophil count observation was conducted using blood smears, then was observed on the first, third and seventh. Group of treatment which was given sodium diclofenac gel as positive control, group of treatment which was without enhancer as the 1st group, groups of treatment which were givenenhancer 1%, 3%, and 5% as the 2nd, 3rd and 4th group. The result was analyzed by one way ANOVA, followed by Post Hoc Duncan. The experimental results prove that there was a significant difference between the number of neutrophils and macrophages in the negative control group who were not given enhancer and the treatment group were given enhancer sodium lauryl sulphate values ( p < 0.05 ). As well as the group treated with the addition of enhancer sodium lauryl sulfate showed a greater amount of decline compared to the treatment group that contains only ethanol extract kencur.
Uji Antipiretik Patch Ekstrak Etanol Bawang Merah (Allium ascalonicum L.) dengan Matriks Chitosan dan Enhancer Tween-80 Willyanto, Jennifer Rachel; Hamid, Iwan Sahrial; Widodo, Teguh
Jurnal Farmasi Sains dan Terapan Vol 5, No 1 (2018)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.838 KB) | DOI: 10.33508/jfst.v5i1.2056

Abstract

Onion is a traditional medicine that has a lot of pharmacological potential. Flavanoid compounds found in onions have an antipyretic effect that acts as an inhibitor of cyclooxygenase (COX) enzymes that play a role in the formation of prostaglandins. In this study red onion is formulated into a patch because of its practical use and can avoid the first metabolic pathway. This study aims to analyze the effect of onion ethanol extract patch (Allium ascalonicum L.) on the decrease of body temperature and the number of monocytes in white rats induced by DPT vaccine. This study used 4 treatment groups: positive controls administered paracetamol syrup, negative controls given placebo patch, treatment group 1 treated with red onion ethanol extract without enhancers and treatment group 2 treated with an red onion ethanol extract with enhancers. Rats adapted for 7 days before temperature being measured and the blood being collected from the rat tail in order to calculate the number of monocytes. The animal temperature was measured after injected with vaccine DPT at 15, 30, 45, 60, 75, 90, 105, 120, 150, 180 minutes meanwhile number of monocyte were calculated every 15 minutes for 120 minutes. The results showed that there was no significant difference in monocyte count of treatment group with positive control but there were significant differences in temperature. The use of onion ethanol extract patch may decrease the temperature and number of monocytes in white mice induced by DPT vaccine.
Uji Antipiretik Patch Ekstrak Etanol Bawang Merah (Allium ascalonicum L.) dengan Matriks Kitosan dan Enhancer SPAN-80 terhadap Temperatur dan Jumlah Makrofag pada Tikus Putih Kariyaningtias, Vivi; Hamid, Iwan Sahrial; Widodo, Teguh
Jurnal Farmasi Sains dan Terapan Vol 5, No 2 (2018)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.906 KB) | DOI: 10.33508/jfst.v5i2.2141

Abstract

Traditional medicine can be used to treat various diseases, one of which is onion (Allium ascalonicum L.) as an antipyretic agent. In this study, the antipyretic potency of ethanol extract of onion (Allium ascalonicum L.) was tested using chitosan and Span-80 as enhancer matrix with temperature and number of intraperitoneal macrophages in white rats as parameters. This study used 20 male white rats Wistar that were induced with DPTHb vaccine intraperitoneally and then divided into four groups namely positive control group (paracetamol), negative control (placebo without extract and without enhancer), formulation 1 (with extract and without enhancer), and formulation 2 (with extracts and enhancers). The results were analyzed using One Way Anova and if the results were significant, then the test continued with Duncan 5%. Correlation calculations were used to determine the relationship between temperature reduction and total intraperitoneal macrophages. The results showed that the temperature reduction did not provide a significant difference between the positive control (paracetamol) and formulation 2 (with extracts and enhancers), whereas in the negative control (without extract and without enhancer) there was no significant difference with formulation 1 (with extract and without enhancers). The total results of intraperitoneal macrophages were significantly different in all groups with a significant> 0.05.
Efektivitas Enhancer Natrium Lauril Sulfat Dalam Patch Topikal Antiinflamasi Ekstrak Etanol Kencur (Kaemferia Galanga L.) Terhadap Jumlah Neutrofil dan Makrofag Pada Mencit Fatmawati, Desy; Dermayati, Cyntia Zain; Hamid, Iwan Sahrial; Hendriati, Lucia
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1970.423 KB) | DOI: 10.35617/jfi.v8i2.517

Abstract

Ethanol extract Kencur (Kaempferia galanga L.) has been proven to have antiinflammatory effect as well as to decrease the side effect of NSAIDs drug use, to develop the formulation of ethanol extract of kencur, then made in the dosage form of topical patch. Topical patch preparations are expected to avoid effects on the gastrointestinal tract, first- pass metabolism, and facilitate the use of the patient thereby increasing patient compliance. In the patch formulation, the most important things to note is the penetration enhancer/ enhancer, and enhancer used in this study is sodium lauryl sulphate. This study aims to determine the effectiveness of sodium lauryl sulphate in the preparation of a topical patch of kencur extract as anti inflammatory on the number of neutrophils in the blood. The research method uses canrrageenan as an inflammation inductor and neutrophil count observation was conducted using blood smears, then was observed on the first, third and seventh. Group of treatment which was given sodium diclofenac gel as positive control, group of treatment which was without enhancer as the 1st group, groups of treatment which were givenenhancer 1%, 3%, and 5% as the 2nd, 3rd and 4th group. The result was analyzed by one way ANOVA, followed by Post Hoc Duncan. The experimental results prove that there was a significant difference between the number of neutrophils and macrophages in the negative control group who were not given enhancer and the treatment group were given enhancer sodium lauryl sulphate values ( p < 0.05 ). As well as the group treated with the addition of enhancer sodium lauryl sulfate showed a greater amount of decline compared to the treatment group that contains only ethanol extract kencur.
ANALGESIC ACTIVITY OF TRANSDERMAL PATCH ETHANOL EXTRACT PIPER NIGRUM L FRUCTUS WITH SOME ENHANCERS ON MICE Hendriati, Lucia; Hamid, Iwan Sahrial; Widodo, Teguh; Surya, Regiskha Hermandanie; Wahyudi, Alif Ekacahya; Rasdianto, Dwi Damayanti
Jurnal Farmasi Sains dan Praktis Vol 7 No 1 (2021)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v7i1.4033

Abstract

Piper nigrum L fructus with active compound piperin had been known have an analgesic effect. Ethanol extract of Piper nigrum formulated to transdermal patch to reduce side effect of analgesic oral. The aim of this research was to know the effect of sodium lauryl sulphate, Tween 80, isopropyl myristate as enhancer of transdermal patch ethanolic extract of Piper nigrum L. Analgesic effect of Piper nigrum L. carried out by writhing method after acetic acid induction in mice. Parameters used were the number of writhing and macrophage from peritoneal fluid of mice at the end of this study. Based on this study, ethanol extract of Piper nigrum had an analgesic effect. The used of sodium lauryl sulphate 5%, Tween 80 5%, isopropyl myristate6% as enhancer increased the analgesic effect of Piper nigrum L.
Uji Aktifitas Antihiperglikemia Ekstrak Etanol 70% Echinometra mathaei Pada Tikus Putih Jantan Galur Wistar Yang Diinduksi Aloksan Kresnamurti, Angelica; Suresti, Suresti; Hamid, Iwan Sahrial
Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS) Vol 2 No 1 (2020): Herclips vol.2 no.1
Publisher : Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30587/herclips.v2i1.2210

Abstract

Abstract : Sea urchin (Echinometra mathaei) contains a compound of Echinochrome A, which is a polyhydroxy naphthoquinone that is known to be potential antioxidant. The antioxidant effect correlated with the antidiabetic activity that associated with oxidative stress conditions. The aim of this study was to determine the effect of 70% Ethanol Extract of sea urchin (Echinometra mathaei) on blood glucose levels in alloxan induced rats. This research was an experimental study using 30 male white rats, divided into 6 groups, i.e the negative control group (normal rat were only given CMC Na 0,5%), Positive control group (hyperglycemic rats were only treated with CMC Na 0,5%), metformin group as a refference standard group (hyperglycemic rats that treated with metformin) and the treatment group (hyperglycemic rats were treated with ethanol extract 70% of Echinometra mathaei dose of 100 mg/kgBW, 200 mg/kgBW and 400 mg/kgBW). Alloxan monohidrat 120 mg/KgBW was used as diabetogen agent. The parameters examined in this study were blood glucose levels of rats after 7 days of treatment. The observational data were analyzed using the One Way ANOVA (P < 0.05) and continued with LSD. The results of this study showed that all doses of ethanol extract significantly reduced blood glucose levels in rats compared to the control group. The most effective dose in reducing blood glucose levels and compared to metformin was 400 mg / kgBW. Key word: alloxan induced, Echinometra mathaei, ethanol 70%, hiperglycemia, marine product.