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UJI POTENSI EKSTRAK DAUN TANAMAN KETEPENG (Cassia alata L) DALAM MENGHAMBAT PERTUMBUHAN BAKTERI Ralstonia solanacearum dan Streptococcus sobrinus Saat Egra; Mardiana Mardiana; Ana Kurnia; Kartina Kartina; Aditya Murtilaksono; Harlinda Kuspradini
ULIN: Jurnal Hutan Tropis Vol 3, No 1 (2019)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32522/ujht.v3i1.2059

Abstract

Ketepeng (Cassia alata L.) was a group of plants included in the Magnoliophyta division which can be found in tropical or subtropical areas. The purpose of this study was to determine the potential of Ketepeng leaves to inhibit the growth of R. solanacearum and S. sobrinus bacteria. The method used in this study is agar well diffusion with 3 replications. The sample used was Ketepeng leaf extract with several concentrations of 0.5%, 1%, 2%. Positive controls in this study were Chloramphenicol and negative control of 40% ethanol. The variables calculated are the calculation of water content, percentage of yield and percentage of area diameter barriers (DDH). In addition, the DDH results show the ethanol extract of Ketepeng leaves at concentrations of 0.5% and 1% not able to inhibit the growth of R. solanacearum, but at a concentration of 2% able to inhibit R.solanacearum with a diameter of 11,7 mm and the ethanol extract of Ketepeng leaves at concentrations was able to inhibit the growth of S. sobrinus bacteria with the highest diameter of 16 mm at a concentration of 2%. 
PENINGKATAN PERTUMBUHAN MISELIUM JAMUR TIRAM (Pleurotus ostreatus) YANG DIPENGARUHI OLEH PROMOL 12 Saat Egra; Muhammad Soesilo Dermawan; Etty Wahyuni; Eko Hary Pudjiwati; Amarullah Amarullah; Dwi Santoso; Deny Murdianto; Sudirman Sirait; Hendris Hendris
ULIN: Jurnal Hutan Tropis Vol 3, No 2 (2019)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.486 KB) | DOI: 10.32522/ujht.v3i2.2889

Abstract

White oyster mushroom (Pleurotus ostreatus) is a mushroom that has a variety of basidiomycetes that grow in tropical forests with high humidity. This mushroom is classified as edible, has even been cultivated for a long time by the community. Therefore, nowadays oyster mushrooms have a high value, it caused many people have cultivated these mushrooms. In this study we want to show a difference in the growth of the white oyster mushroom mycelium before given promol 12 (as control) and after being given 12 types of local microbial probiotics (PROMOL12). The method used is RAK (Design random groups). In this study, it was shown that the influence of the growth of white oyster mushroom mycelium was given PROMOL12. The growth of oyster mushrooms showed a difference for baglog 12 promol medium having an average growth of 0.6 cm and a growth period of 14 days, on the other hand baglog media control had an average growth of 0.7 cm with a growth period of 17 days. With the same environmental conditions, the average temperature is 27-29.5OC and the humidity is also not less than 60%, which has an average of 70% -81% which is in accordance with the growth of oyster mushroom mycelium.
POTENSI JAMUR TIRAM PUTIH (Pleurotus ostreatus) TERHADAP PENGHAMBATAN Candida albicans DAN Propionibacterium acnes Saat Egra; Irawan Wijaya Kusuma; Enos Tangke Arung
ULIN: Jurnal Hutan Tropis Vol 2, No 1 (2018)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.928 KB) | DOI: 10.32522/ujht.v2i1.1045

Abstract

Oyster mushroom (Pleurotus ostreatus) is not a new food for the community. Long time ago oyster mushrooms is food but not so well known. Now oyster mushrooms have been accepted by the community as a healthy food. This is the background of this research to produce mushrooms not only as nutritious food but also natural medicine. The purpose of this study was to examine the antimicrobial potential of oyster mushrooms by determination of clear zone against Candida albicans and Propionibacterium acnes bacterias. This research was conducted at Forest Products Chemistry Laboratory, Faculty of Forestry, Mulawarman University. The resources of oyster mushroom we used in this study comes from the cultivation of the entrepreanure forestry student group in Mulawarman University. This study used successful extraction with hexane solvent, ethyl acetate, ethanol, water, and crude ethanol. The results obtained that the highest antimicrobial inhibition against Candida albicans bacteria with 47.60% with a concentration of 100 ppm but, on the antimicrobial against Propionibacterium acnes bacteria did not show any significant inhibition.
KANDUNGAN ANTIOKSIDAN PADA JAMUR TIRAM PUTIH (Pleurotus ostreatus) Saat Egra; Irawan Wijaya Kusuma; Enos Tangke Arung
ULIN: Jurnal Hutan Tropis Vol 2, No 2 (2018)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.672 KB) | DOI: 10.32522/ujht.v2i2.1549

Abstract

Indonesia merupakan negara hutan tropis yang luas, maka tak heran negara ini menjadi negara megabiodiversitas. Jamur tiram merupakan salah satu hasil kekayaan alam Indonesia yang memiliki potensi sebagai tanaman kesehatan dan obat. penelitian ini bertujuan untuk memunculkan potensi aktivitas antioksidan pada jamur tiram yang banyak dibudidaya di Kalimantan Timur khususnya Samarinda. Sampel kami ambil dari rumah jamur borneo dan penelitian ini dilaksanakan di Laboratorium Kimia Hasil Hutan, Fakultas Kehutanan, Universitas Mulawarman. Metode ekstraksi bertingkat menggunakan n-heksan, etil asetat, dan etanol. Metode antioksidan yang digunakan adalah penangkal radikal bebas (DPPH) dengan masing-masing tiga kali pengulangan. Hasil penelitian ini menunjukkan bahwa aktivitas antioksidan tertinggi pada ekstrak air dengan nilai penghambatan 25% pada kosentrasi 100 ppm. Pada ekstrak heksan merupakan hambatan terkecil dengan nilai penghambatan 2,41% pada kosentrasi 25ppm. 
Aktivitas Antimikroba Ekstrak Bakau (Rhizophora mucronata) dalam Menghambat Pertumbuhan Ralstonia solanacearum Penyebab Penyakit Layu Saat Egra; Mardhiana Mardhiana; Mut Rofin; Muhammad Adiwena; Nur Jannah; Harlinda Kuspradini; Tohru Mitsunaga
Agrovigor Vol 12, No 1 (2019): Maret
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.853 KB) | DOI: 10.21107/agrovigor.v12i1.5143

Abstract

R. mucronata telah banyak digunakan sebagai tanaman obat-obatan tradisional karena mampu menghasilkan metabolit sekunder seperti tanin, alkaloid, terpenoid, sapponin dan flavonoid. Penelitian ini bertujuan untuk mengetahui potensi bagian tanaman daun dan kulit batang sebagai penghambat bakteri R. solanacearum, dengan konsentrasi 5000 ppm, 10000 ppm dan 20000 ppm, kontrol positif (Chlorampenicol), kontrol negatif (Etanol). Metode yang digunakan untuk uji daya hambat menggunakan metode difusi agar sumuran. Hasil penelitian menunjukan faktor kelembaban daun R. mucronata (0.33g) kulit batang (0.58g). Hasil rendemen ekstrak daun (17.61%) kulit batang (7.85%). Persentase penghambatan menunjukkan bahwa ekstrak daun R. mucronata memiliki daya hambat terhadap R. solanacearum pada konsentrasi 20000 ppm dan 10000 ppm masing-masing 31% dan 29%, namun disisi lain pada daun R. mucronata konsentrasi 5000 ppm tidak ada aktivitas daya hambat. Pada sampel yang berbeda Kulit batang R. mucronata menunjukkan adanya aktivitas daya hambat pada konsentrasi 5000 ppm, 10000 ppm dan 20000 ppm dengan nilai persentase secara berturut-turut 34%, 39%, dan 44%.