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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) JSDH BERITA BIOLOGI Jurnal Biodjati BIOEDUSCIENCE Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Menara Perkebunan JPPI (Jurnal Penelitian Pendidikan Indonesia) MADURANCH: JURNAL ILMU PETERNAKAN Jurnal Ilmiah Sains
Hamim Hamim
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Mathematics Physics Veterinary

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REKAYASA EKSPRESI GEN PEMBUNGAAN Hd3a DIBAWAH KENDALI PROMOTER ROL C PADA JARAK PAGAR (Jatropha curcas L.) Sulistyaningsih, Yohana C; Hartana, Alex; Widyastuti, Utut; Hamim, Hamim; Suharsono, Suharsono
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.374 KB) | DOI: 10.14203/beritabiologi.v10i6.1940

Abstract

Flowering in jatropha (Jatropha curcas L.) was considered as one of major factors that contribute to its productivity. Small number of female flowers produced in each inflorescence was believed as the main cause of low seed production.Introduction of Hd3a flowering gene driven by rol C promoter was supposed to improve total number of flowers including female flower.The objective of this research was to optimize cell proliferation and regeneration medium in Jatropha transformation method mediated by Agrobacterium, to obtain transgenic Jatropha containing Hd3a flowering gene as well as to understand the effect of this transgene on jatropha flowering character.Callus induction medium containing 0.5 mg/1 IBA added with 3 g/1 PVP produced the highest frequency of shoot formation.We obtained 26.67% to 33.33% putative transgenic plantlets that were able to grow in 40mg/l hygromycin selection medium. PCR analysis revealed that seven out of 10 putative transgenic plantlets were positively transgenic.Extremely early flowering character that was confirmed by histological analysis was also shown by some transgenic plantlets.
Klorofil Floresen dari Spesies C3 dan C4 dalam Responnya terhadap Cekaman Kekeringan Hamim, Hamim
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.214 KB) | DOI: 10.24002/biota.v10i3.2876

Abstract

Klorofil floresen fotosintesis dari dua spesies C3 (gandum dan kale) dan dua spesies C4 (Echinochloa cursgallii dan Amaranthus caudatus) dianalisis dalam responnya terhadap cekaman kekeringan di rumah kaca. Tumbuhan ditanam dalam pot berdiameter 15 cm selama sebulan kemudian diberi perlakuan kekeringan dengan penundaan penyiraman hingga tumbuhan layu. Kuantum efisiensi maksimum dari sistem cahaya II fotosintesis (Fv/Fm), quenching foto kimia (qP) dan non-fotokimia (qN) dianalisis untuk mengetahui keadaan fotosintesis tumbuhan selama cekaman kekeringan. Walaupun tidak ada pola yang jelas dalam hal status air dari spesies C3 dan C4, cekaman kekeringan yang diberikan menyebabkan penurunan kadar air medium (MWC), potensial air (WP) dan potensial osmotik (OP) semua spesies.  Gandum mamiliki nilai WP dan OP yang paling rendah sementara E. crusgallii memiliki nilai yang paling tinggi akibat cekaman kekeringan. Kekeringan menyebabkan penurunan laju fotosintesis pada semua spesies yang ditandai dengan penurunan qP, namun hanya kale dan A. caudatus yang mengalami peningkatan qN akibat cekaman kekeringan. Tetap stabilnya qP dari E. crusgallii pada awal cekaman mungkin berkaitan dengan mekanisme C4 yang dimiliki oleh spesies ini.  Penurunan Fv/Fm pada E. crusgallii pada periode akhir cekaman menunjukkan bahwa spesies ini mengalami fotoinhibisi disebabkan cekaman kekeringan.
Enkapsulasi dan Regenerasi Kalus Embriogenik Mangga (Mangifera indica L.) Kultivar Bapang dan Gadung 21 Ermayanti, Tri Muji; Nugroho, Robertus; Hamim, Hamim
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.033 KB) | DOI: 10.24002/biota.v15i3.2598

Abstract

The mother plant and genetic variability of Indonesian mango need to be conserved. Encapsulation is one of in vitro conservation used for many plant species. The aim of this research was to study the regeneration of encapsulated mango cultivar Bapang and Gadung 21 embryogenic callus after storage at -14, 3−5, and 26−27oC for 0, 1, 2, 4, and 8 weeks. Embryogenenic callus was treated with 3% Na-alginate (in liquid 3M), then it was dropped into 100 mM CaCl2. Encapsulated callus beads were dehidrated and stored at -14, 3−5, and 26−27oC for 0, 1, 2, 4, and 8 weeks. After storage, the callus was cultured in 3M medium containing 2,4-D at 0, 1, and 2 mg/l for the regeneration. The results showed that after 8-week storage, callus of Bapang culticar was more viable (67.3%) and resulted more number of somatic embryos (191.6%) than Gadung 21 cultivar. The callus which was cultured in 3M medium without addition of 2,4-D was more viable (20.9%) and had more number of somatic embryos (1062.5%) than that which was cultured on medium containing 2,4-D. After 2-week storage, callus had viability of 7.6%. No storage callus formed more somatic embryos than storage callus. Storage at 26−27oC gave higher viability as well as higher number of somatic embryo than stored at -14 and 3−5 oC. The callus did not regenerate into shoots after storage at -14oC. Embryogenic callus could be stored at 3−5 and 26−27oC for 4 weeks.
The expression of OsPLA2-III and OsPPO genes in rice (Oryza sativa L.) under Fe toxicity stress Turhadi, .; Hamim, .; Ghulamahdi, Munif; Miftahudin, .
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

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Abstract

Lipid is an important biomolecule in plant because of their structural and functional roles in plant cells. Moreover, lipid could be a signal molecule in defense system of plants under biotic and abiotic stresses. Detoxification process of toxic substances, which is one of the defense system and controlled by various genes, plays an important role in tolerance mechanism in plant under stress conditions, including iron (Fe) toxicity. Plants develop various tolerance strategies to cope the Fe toxicity. The objective of this research was to investigate the expression of OsPLA2-III and OsPPO genes under Fe stress condition. Two-weeks-old seedlings of two rice varieties, i.e., IR64 (Fe-sensitive toxicity) and Pokkali (Fe-tolerant toxicity) were used. Both varieties were treated with 1 (control) and 400 ppm of Fe in the form of FeSO4.7H2O in the nutrient culture solution. The leaf bronzing, chlorophyll content, and relative expression of OsPLA2-III and OsPPO genes were observed. An in-silico study was also performed to predict the interaction between OsPLA2-III and OsPPO proteins. The results showed that the Fe toxicity induced leaf bronzing, reduced leaf chlorophyll content, and increased the OsPLA2-III and OsPPO gene expression levels. Both genes are suggested to have a role in plant tolerance mechanism during Fe toxicity stress via lipid signaling pathway.
Physiological Responses and Fruit Retention of Carambola Fruit (Averrhoa carambola L.) Induced by 2,4-D and GA3 BEKTI KURNIAWATI; HAMIM HAMIM
HAYATI Journal of Biosciences Vol. 16 No. 1 (2009): March 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.753 KB) | DOI: 10.4308/hjb.16.1.9

Abstract

One of the problems in cultivation of carambola fruit is the high of flower and fruit drop during fruit development. To understand these problems and to improve fruit retention, the content of indole-3-acetic acid (IAA) and total sugar in carambola fruit and leaves were analysed in response to application of gibberellic acid (GA3) and 2,4-dichlorophenoxyacetic acid (2,4-D). The experiments used 1,5 year old of carambola plants (Averrhoa carambola L. var Dewi) grown in polybag of 40 x 50 cm. GA3 with the concentration of 0, 20, 40, and 60 ppm and 2,4-D of 0, 5, 10, and 15 ppm were applied to the flower and the supporting leaves of carambola plant. The parameters analysed were number of flower drop, fruit formation, fruit retention, number of harvestable fruit per cluster, fruit weight per cluster, the content of sugar in the leaves and IAA in the fruit. The result showed that IAA content of the fruit increased in response to single as well as combination of GA3 and 2,4-D application. Sugar content of the leaves also increased in response to GA3 and 2,4-D application; however, the pattern was different with that of IAA. The best treatment to improve fruit retention was a single application of 10 ppm 2,4-D or 60 ppm GA3, and combined application of 5 ppm 2,4-D and 60 ppm GA3. Key words: fruit drop, fruit retention, carambola fruit, auxin, gibberellin
Photosynthesis of C3 and C4 Species in Response to Increased CO2 Concentration and Drought Stress HAMIM HAMIM
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.934 KB) | DOI: 10.4308/hjb.12.4.131

Abstract

Photosynthetic gas exchange in response to increased carbon dioxide concentration ([CO2]) and drought stress of two C3 (wheat and kale) and two C4 species (Echinochloa crusgallii and Amaranthus caudatus) were analysed. Plants were grown in controlled growth chambers with ambient (350 mol mol-1) and doubled ambient [CO2]. Drought was given by withholding water until the plants severely wilted, whereas the control plants were watered daily. Even though stomatal conductance (Gs) of C4 species either under ambient or double [CO2] was lower than those in C3, doubled [CO2] decreased Gs of all species under well watered conditions. As a result, the plants grown under doubled [CO2] transpired less water than those grown under ambient [CO2]. Photosynthesis (Pn) of the C4 species was sustained during moderate drought when those of the C3 species decreased significantly. Doubled [CO2] increased photosynthesis of C3 but not of C4 species. Increased [CO2] was only able to delay Pn reduction of all species due to the drought, but not remove it completely. The positive effects of increased [CO2] during moderate drought and the disappearance of it under severe drought suggesting that metabolic effect may limit photosynthesis under severe drought.
Overexpression of B11 Gene in Transgenic Rice Increased Tolerance to Aluminum Stress Devi Media Siska; . Hamim; . Miftahudin
HAYATI Journal of Biosciences Vol. 24 No. 2 (2017): April 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1789.324 KB) | DOI: 10.4308/hjb.24.2.96

Abstract

Rice cultivation on acid soils is mainly constrained by aluminum (Al) toxicity. However, rice has tolerance mechanism to Al stress, which is controlled by many genes. B11 gene is one of the Al- tolerance gene candidate isolated from rice var. Hawara Bunar. It has not been known whether overexpression of the gene in Al-sensitive rice is able to increase Al tolerance. The research objective was to analyze root morphological and physiological responses of transgenic rice overexpressing B11 gene to Al stress. The experiment was carried out using five rice genotypes including two varieties (Hawara Bunar and IR64) and three T4 generation of transgenic lines, that are T8-2-4, T8-12-5, and T8-15-41. All rice genotypes were grown in nutrient solution for 24 h (adaptation period), and then were exposed to 15 ppm Al for 72 h (treatment period) and recovered in normal nutrient solution for 48 h (recovery period). The result showed that the overexpression of the B11 gene in T8-2-4, T8-12-5, and T8-15-41 transgenic lines improved tolerance to Al stress based on root growth characters, accumulation of Al, root cell membrane lipid peroxidation, and root tip cell structure.
Impact of Methanotrophic and N2O-reducing Bacterial Inoculation on CH4 and N2O Emissions, Paddy Growth and Bacterial Community Structure in Paddy Field Yuli Siti Fatma; Iman Rusmana; Aris Tri Wahyudi; Hamim Hamim
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.197

Abstract

Paddy field is one of the anthropogenic sources that produce greenhouse gases emission. This study aimed to investigate the impact of methanotrophic and N2O-reducing bacterial inoculation on CH4 and N2O emissions, paddy growth and bacterial community structure in paddy field. Two treatments of 100% synthetic fertilizer (250 kg urea/ha) without biofertilizer and 50% synthetic fertilizer (125 kg urea/ha) with biofertilizer consisted of methanotrophic and N2O-reducing bacteria were applied in the paddy field. Inoculation of methanotrophic and N2O-reducing bacteria was able to reduce CH4 and N2O emission up to 4.19 mg CH4/m2/day and 351.29 µg N2O/m2/day, respectively. Those bacterial applications were also able to increase paddy growth and yield productivity. According to DGGE profile, inoculation of the biofertilizer seemed to have a transient impact on bacterial communities in paddy soil at 36 days after transplanting (DAT) which showed the lowest similarity with all samples (a similarity index of 0.68). DGGE bands successfully excised have closest relative to uncultured bacteria which comprised 5 phyla, i.e. Proteobacteria (Alphaproteobacteria and Deltaproteobacteria), Nitrospirae, Actinobacteria, Firmicutes, and Acidobacteria. In this study, Alphaproteobacteria was the most dominant phylum. We provide basic information for developing the biofertilizer which supports sustainable agriculture.
Gold Mine Wastewater Induced Morpho-physiological Alteration of Four Biodiesel Producing Species Hamim Hamim; Lutfiani Lutfiani; Deden Saprudin; Putri Nur Azizah Hutagalung; Hadisunarso Hadisunarso; Luluk Setyaningsih; Hirmas Fuady Putra
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.63

Abstract

This experiment aimed to determine the adaptability of four biodiesel-producing plants to gold mine wastewater based on morpho-physiological properties. Four species namely jatropha (Jatropha curcas), castor bean (Ricinus communis), wild candlenut (Reutealis trisperma) and bead tree (Melia azedarach) were grown in water culture using Hoagland solution for 2 weeks and then treated with gold mining wastewater at 0% (control), 25%, 50%, and 75% (v/v) for 3 weeks. Growth, anatomical and physiological characteristics were observed during the treatment. The results showed that gold mine wastewater significantly decreased growth of the four species despite varied among the species. Wastewater had significant effect on leaves anatomy of Jatropha but not on others. It also significantly induced the increase of malondialdehyde (MDA) content in Jatropha and castor bean, but not in wild candlenut and bead-tree. Meanwhile, the treatment significantly decreased chlorophyll content of all species with the most in bead-tree plant, and leaves relative water content (RWC) particularly in castor bean and bead-tree plants. There was strong negative correlation between the increase of MDA content and the decrease of chlorophyll content and leaf RWC. Among the four species, wild candlenut (R. trisperma) was the most resistant to gold mine wastewater based on morphological and physiological properties.
TOLERANSI TANAMAN KEDELAI TERHADAP CEKAMAN AIR : AKUMULASI PROLIN DAN ASAM ABSISIK DAN HUBUNGANNYA DENGAN POTENSIAL OSMOTIK DAUN DAN PENYESUAIAN OSMOTIK Didy Sopandie; , Hamim; Muhammad Jusuf; Nani Heryani
Indonesian Journal of Agronomy Vol. 24 No. 1 (1996): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (991.078 KB) | DOI: 10.24831/jai.v24i1.1616

Abstract

In this experiment. the changes on leaf osmotic potential and accumulation of proline and abscisic acid were identified from drought-tolerant and drought - sensitive soybean genotypes. Three drought - tolerant (Mlg 2805, Mlg 2984 and Mlg 2999) and two sensitive soybean genotypes (Mlg 2510 and Mlg 3541) were subjected to drought condition created by regulating water supply in greenhouse.  The results revealed that exposing plants to drought stress brought about a decrease of leaf osmotic potential. The decrease of which was greater in drought-tolerant genotypes (6.91 to 10.11 bars) than in sensitive genotypes (0.55 to 0.69 bars). The decreasing of leaf osmotic potential was followed with increasing praline accumulation, especialy for Mlg 2805. Only Mlg 2805 showed the significant ABA accumulation when the plants were subjected to drought stress. It is suggested that the drought tolerance was associated with the reduction of leaf osmotic potential (osmotic adjusment) in which proline might play an important role. The role of ABA could not be clarified since there had been a great variability in ABA content of all tolerant genotypes.
Co-Authors , Triadiati Alex Hartana ALEX HARTANA Alex Hartana Aris Tri Wahyudi BEKTI KURNIAWATI Deden Saprudin Devi Media Siska Dian Hendrayanti Didy Sopandie Dorly Dorly DWI ANDREAS SANTOSA Hadisunarso Hadisunarso Hirmas Fuady Putra HIRMAS FUADY PUTRA, HIRMAS FUADY Iman Rusmana Inayatur Rosyidah Indah Puspita SARI Laksmita Prima SANTI Luluk Setyaningsih Lutfiani Lutfiani Miftahuddin Miftahudin . Mubasyiroh, Mubasyiroh Muhammad Jusuf MUNIF GHULAMAHDI Nampiah Sukarno Nani Heryani NISA RACHMANIA MUBARIK Nugroho, Robertus Nurul Hidayati Putri Nur Azizah Hutagalung Rani Apriyani Raharja Robertus Nugroho Sigit Tri Wibowo Smeer, Zeid B Sri Wilarso Budi R Suharsono Suharsono Sukmarayu P Gedoan Sukmarayu P. Gedoan Sulistyaningsih, Yohana C Tri Muji Ermayanti Tri Muji Ermayanti Turhadi Turhadi Utut Widyastuti UTUT WIDYASTUTI UTUT WIDYASTUTI Yadi Setiadi Yohana Caecilia Sulistyaningsih Yuli Siti Fatma YULIN LESTARI Zahrul Romadlon