Wuryanti Handayani
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jember. Center for Development of Advanced Sciences and Technology, Universitas Jember

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Optimization pH of Enzymatic Hydrolysis of Endo-1,4-β-Xylanase for Xylooligosaccharides Production Ratnadewi, Anak Agung Istri; Kurniawan, Andika Ade; Handayani, Wuryanti
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Xylooligosaccharides (XOS) with polymerisation degree between 2 till 10 monomer  have prebiotic effect for better digestion system. In this research, production of XOS was performed by enzymatic hydrolysis of xylan with endo-1,4-β-xylanases enzyme. Endo 1,4-β-xylanases enzyme was aqcuired from Bacillus sp. isolated from termite’s abdominal.Meanwhile oat xylan was used as substrate. Optimal condition of enzymatic hydrolysis was evaluated at pH: 4, 5, 6, and 7 with incubation time from 5 to 20 h.pH 5 was optimum pH to produce XOS from 0.8 % xylan oat at 40 oC.The hydrolysis product purified and analyzed by thin layer chromatographyyielding spot of xylobiose, xylotriose, xilotetraose and xilopentaose. Further analysis by HPLC indicated dominant xilopentaosa X5 (3522 ppm) among  the other XOS   X2 (14 ppm), X3 (43 ppm) and X4 (15 ppm). Keywords: Xylooligosaccharides, endo-1,4-β- endoxylanase
OPTIMASI KONSENTRASI SUBSTRAT XILAN AMPAS TAHU TERHADAP ENDO-Î’-1,4-D-XYLANASE UNTUK MEMPRODUKSI XILOOLIGOSAKARIDA Ratnadewi, Anak Agung Istri; Handayani, Wuryanti; Avida, Siti Nur
Jurnal Kimia Riset Vol 1, No 2 (2016): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.779 KB) | DOI: 10.20473/jkr.v1i2.3084

Abstract

AbstrakAmpas tahu merupakan limbah samping dari proses pengolahan tahu dan susu kedelai. Ampas tahu berpotensi sebagai sumber xilan. Xilan digunakan sebagai substrat endo-β-1,4-D-xilanase untuk menghasilkan xilooligosakarida. Penelitian ini digunakan xilan ampas yang telah dihilangkan lemak dan protein tanpa penghilangan lignin (X1nD). Xilan ampas tahu tanpa penghilangan lemak dan protein tetapi dilakukan penghilangan lignin (X2D). Enzim yang digunakan adalah endo-β-1,4-D-xilanase dari isolat Bacillus sp. asal abdomen rayap. Optimasi variasi konsentrasi substrat bertujuan untuk mengetahui konsentrasi optimum dalam menghasilkan xilooligosakarida. Produk hidrolisis yang dihasilkan dianalisis menggunakan metode Miller untuk mengetahui total gula pereduksi. Produk hidrolisis konsentrasi optimum dianalisis menggunakan Kromatografi Lapis Tipis (KLT) untuk mengetahui komponen penyusun xilooligosakarida. Substrat X1nD dan X2D optimum pada konsentrasi 6% dan 5% dengan total gula pereduksi sebesar 0,196 mg/ml dan 0,211 mg/ml. Komponen penyusun xilooligosakarida ampas tahu berupa xilotriosa (X3), xilotetraosa (X4), dan xilopentaosa (X5).Kata Kunci: Ampas tahu, endo-β-1,4-D-xilanase, xilan, xilooligosakarida. AbstractOkara is a waste byproduct of the processing of tofu and soy milk. Okara potential as a source of xylan. Xylan is used as the substrate endo-1,4-β-D-xylanase to produce xyloologosaccharide. This study used okara xylan had eliminated fat and protein without removal of lignin (X1nD). Okara xylan out without the removal of fat and protein but do removal of lignin (X2D). The enzyme used is endo-1,4-β-D-xylanase of isolates of Bacillus sp. From abdominal termites. Optimization of substrate concentration variation aims to determine the optimum concentration in generating xyloologosaccharide. Hydrolysis products were analyzed using Miller method to determine total reducing sugars. The optimum concentration of hydrolysis products were analyzed using Thin Layer Chromatography (TLC) to determine the components of xyloologosaccharide. X1nD and X2D optimum substrate at a concentration of 6% and 5% to the total reducing sugars of 0.196 mg/ml and 0.211 mg/ml. Xyloologosaccharide of okara components of the pulp out the form xylotriose (X3), xylotetraose (X4), and xylopentaose (X5).Keywords: Okara, endo-1,4-β-D-xylanase, xylan, xyloologosaccharide
Production and Characterization of Enzyme β-Endoxylanase from Bacteria of Termite-intestinal System Ratnawati, A. A. Istri; Handayani, Wuryanti; Puspaningsih, Ni Nyoman Tri
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

A mesophile bacteria isolated from intestinal systems of local-soil termite, produced an extracellular β-endoxylanase upon growth on xylan. Optimum production of the enzyme was found in media containing oatspelt xylan at 37oC after sixth hours. The activities of its β-endoxylanase on oat-spelt xylan was investigated. It had an optimum pH and temperature, 5.0 and 40o C, respectively. However, pH stability occurred between 5.08.0. The enzyme was stable at 40o C for four hours and possessed a half life of four hours. β-endoxylanase had an apparent molecular mass of 45.000 to 66.200 Dalton as determined by SDS-PAGE. Analysis of zymogram using SDS-Xylan-PAGE indicated that enzymes could degrade oat-spelt xylan as substrates.
Screening Fitokimia dan Studi Aktivitas Ekstrak Daun Sintok (Cinnamomum sintoc Bl.) Sebagai Antioksidan dan Antihiperlipidemia Kumalasari, Ardine; Handayani, Wuryanti; Siswoyo, Tri Agus
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9916

Abstract

Cinnamomum sintoc Bl. merupakan tanaman yang memiliki kandungan minyak atsiri di kulit batangnya yang digunakan untuk antioksidan, antihiperlipidemia dan lain-lain. Dari penelitian ini, senyawa metabolit sekunder dari daun sintok (kadar air 54,7%±0,69) diekstrak dengan metanol dan dianalisis dengan reagen spesifik. Daun sintok diekstraksi secara maserasi bertingkat dengan meningkatkan kepolaran pelarut, yaitu n-heksana, etil asetat, metanol. Uji antioksidan dan antihiperlipidemia dilakukan pada setiap ekstrak(HS, ES, MS) menggunakan standar asam galat equivalent. Total fenolik dan total flavonoid dihitung menggunakan kurva standar asam galat dan kuersetin, hasil total fenolik dari setiap ekstrak antara lain HS (39,23±2,79 mg AGE/g); ES (110,77±2,37 mg AGE/g); dan MS (283,63±3,96 mg AGE/g). Aktivitas antioksidan pada ekstrak ditentukan dengan kemampuan ekstrak untuk meredam DPPH sedangkan aktivitas antihiperlipidemia ekstrak ditentukan dengan kemampuan ekstrak untuk menghambat kinerja lipase. Potensi ekstrak daun sintok terhadap antioksidan cukup tinggi hanya untuk ekstrak MS, sedangkan potensi terhadap antihiperlipidemia untuk semua jenis ekstrak. Kata Kunci: Cinnamomum sintoc Bl., fitokimia, maserasi, fenolik, antioksidan, antihiperlipidemia.
Ekstraksi Xilan dari Limbah Ampas Singkong dan Pemanfaatannya sebagai Substrat Endo-B-1,4-D-Xilanase Firdausa, Fita Kurnia; Santoso, Agung Budi; Handayani, Wuryanti
BERKALA SAINSTEK Vol 5 No 1 (2017)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v5i1.5376

Abstract

Ampas singkong merupakan limbah hasil pembuatan tepung tapioka. Hemiselulosa merupakan komponen kimia pada ampas singkong, komponen utama hemiselulosa adalah xilan. Penelitian ini bertujuan mendapatkan xilan dari ampas singkong serta pemanfaatan xilan sebagai substrat endo-B-1,4-D-xilanase. Ampas singkong yang akan diekstraksi diukur terlebih dahulu kadar HCN, lignin, dan air. Ekstraksi xilan dari ampas singketode DNS. Hasil penelitian menunjukkan bahwa ampas singkong yang digunakan pada penelitian ini memiliki kandungan HCN sebesar 16,10 ppm, kadar lignin sebesar 4,34% dan kadar air sebesar 7,45%. Ekstraksi xilan dengan NaOH 12% menghasilkan rendemen tertinggi yaitu 32,14% (delignifikasi) dan 52,36% (tanpa delignifikasi).Kata Kunci: Ampas singkong, xilan, delignifikasi.
Screening Fitokimia dan Studi Aktivitas Ekstrak Daun Sintok (Cinnamomum sintoc Bl.) Sebagai Antioksidan dan Antihiperlipidemia Kumalasari, Ardine; Handayani, Wuryanti; Siswoyo, Tri Agus
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9683

Abstract

Cinnamomum sintoc Bl. merupakan tanaman yang memiliki kandungan minyak atsiri di kulit batangnya yang digunakan untuk antioksidan, antihiperlipidemia dan lain-lain. Dari penelitian ini, senyawa metabolit sekunder dari daun sintok (kadar air 54,7%±0,69) diekstrak dengan metanol dan dianalisis dengan reagen spesifik. Daun sintok diekstraksi secara maserasi bertingkat dengan meningkatkan kepolaran pelarut, yaitu n-heksana, etil asetat, metanol. Uji antioksidan dan antihiperlipidemia dilakukan pada setiap ekstrak(HS, ES, MS) menggunakan standar asam galat equivalent. Total fenolik dan total flavonoid dihitung menggunakan kurva standar asam galat dan kuersetin, hasil total fenolik dari setiap ekstrak antara lain HS (39,23±2,79 mg AGE/g); ES (110,77±2,37 mg AGE/g); dan MS (283,63±3,96 mg AGE/g). Aktivitas antioksidan pada ekstrak ditentukan dengan kemampuan ekstrak untuk meredam DPPH sedangkan aktivitas antihiperlipidemia ekstrak ditentukan dengan kemampuan ekstrak untuk menghambat kinerja lipase. Potensi ekstrak daun sintok terhadap antioksidan cukup tinggi hanya untuk ekstrak MS, sedangkan potensi terhadap antihiperlipidemia untuk semua jenis ekstrak.
Essential Oil Composition of Rose Flowers from Karangpring Village Jember District Extracted by Distillation and Enfleurage Oktavianawati, Ika; Letisya, Nanda; Citra, Priscillia; Utari, Dwi Purwita; Winata, I Nyoman Adi; Handayani, Wuryanti; Nugraha, Ari Satya
Jurnal ILMU DASAR Vol 20 No 2 (2019)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.523 KB) | DOI: 10.19184/jid.v20i2.8995

Abstract

Karangpring is one tourist destination villages in Jember district which is popular with beautiful large rose field areas. Therefore, this area grows to be a leading producer of rose flowers in Jember district. However, the bulky presence of these flowers made its price becomes lower in regular days. Local community only uses and sells these fresh flowers as the flower for funeral. The rose flower has a great potency to be explored as a source of rose essential oil production. To date, there is no previous research on studying rose flowers from Karangpring village for its potency on the essential oil production. In this research, rose flowers were subjected to be extracted of its essential oil using two extraction methods, distillation, and enfleurage. Hydrodistillation resulted two phases of distillates, above part formed a cloudy white phase as a normal essential oil extracted from plants, and the lower phase was an aqueous phase containing rose hydrosols. Both phases of these condensates were analyzed using GCMS. Data explained that above phase, with a yield oil of 0.07%, only contains long-chain hydrocarbons such as n-nonadecane, n-heptadecane, 9-nonadecene, and eicosane, while the lower phase only contains 2-phenylethyl alcohol. On the other hand, enfleurage of fresh rose flowers resulted in 0.06% oil yield. GCMS analysis of this oil shows that 2-phenylethyl alcohol, eugenol, and phenylacetic acid are three major compounds which take more than 85% of total rose absolute. The results show that enfleurage is a better method for extracting rose oil in better quality than using the distillation method, in term of the variety of volatile components. Meanwhile, hydrodistillation is still benefiting from producing rose water that is qualified as an industrial additive agent for food and cosmetic productions or even a new potent of agromedicine products. Keywords: rose, rose oil, rose water, rose absolute, distillation, enfleurage.
Optimization pH of Enzymatic Hydrolysis of Endo-1,4-β-Xylanase for Xylooligosaccharides Production Ratnadewi, Anak Agung Istri; Kurniawan, Andika Ade; Handayani, Wuryanti
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Xylooligosaccharides (XOS) with polymerisation degree between 2 till 10 monomer  have prebiotic effect for better digestion system. In this research, production of XOS was performed by enzymatic hydrolysis of xylan with endo-1,4-β-xylanases enzyme. Endo 1,4-β-xylanases enzyme was aqcuired from Bacillus sp. isolated from termite’s abdominal.Meanwhile oat xylan was used as substrate. Optimal condition of enzymatic hydrolysis was evaluated at pH: 4, 5, 6, and 7 with incubation time from 5 to 20 h.pH 5 was optimum pH to produce XOS from 0.8 % xylan oat at 40 oC.The hydrolysis product purified and analyzed by thin layer chromatographyyielding spot of xylobiose, xylotriose, xilotetraose and xilopentaose. Further analysis by HPLC indicated dominant xilopentaosa X5 (3522 ppm) among  the other XOS   X2 (14 ppm), X3 (43 ppm) and X4 (15 ppm). Keywords: Xylooligosaccharides, endo-1,4-β- endoxylanase