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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Teknologi Pertanian JURNAL PERIKANAN TROPIS
Kustiariyah Tarman
Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology

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SENYAWA METABOLIT SEKUNDER KAPANG ENDOFIT TP6 DAN TPL2 YANG DIISOLASI DARI TUMBUHAN PESISIR TERONG PUNGO (Solanum sp.) Nabila Ukhty; Kustiariyah Tarman; Iriani Setyaningsih
JURNAL PERIKANAN TROPIS Vol 2, No 2 (2015)
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.112 KB) | DOI: 10.35308/jpt.v2i2.26

Abstract

Secondary metabolites, are produced naturally and serve survival functions for the organisms that producing them. Endophytic fungi is the one of microorganism has a role in producing the secondary metabolites. Endophytic fungi TP6 and TPL2 isolated from coastal plant terong pungo (Solanum sp.). Endophytic fungi TP6 and TPL2 have secondary metabolite, including alcaloid, flavonoid, phenol hidrocuinon, and terpenoid compounds.
STUDI PENERIMAAN KONSUMEN PADA MINUMAN SERBUK LINTAH LAUT (Discodoris sp.) R. Marwita Sari Putri; Nurjanah Nurjanah; Kustiariyah Tarman
JURNAL TEKNOLOGI PERTANIAN Vol. 2 No. 1 (2013)
Publisher : Prodi Teknologi Pangan Fakultas Pertanian Universitas Islam Indragiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32520/jtp.v2i1.46

Abstract

Sumber daya perairan yaitu lintah laut (Discodoris sp.) dapat dibuat menjadi minuman fungsional. Lintah laut telah dilaporkan memiliki sifat antioksidan dan mengandung taurin. Tujuan dari penelitian ini adalah untuk mengetahui penerimaan konsumen pada minuman serbuk fungsional lintah laut. Tiga formula terbaik yang diterima secara organoleptik yaitu Formula T1 (Discodoris sp. 20%, jahe 40%, 20% curcuma, lemon 20%.), T2 Formula (Discodoris sp. 25%, jahe 40%, 15% curcuma, lemon 20%) dan T3 Formula (Discodoris sp. 30%,. jahe 40%, 10% curcuma, lemon 20%).
Fatty Acid Profile, Carotenoid Content, and In Vitro Anticancer Activity of Karimunjawa and Lampung Sea Cucumber Ekowati Chasanah; Yusro Nuri Fawzya; Kustiariyah Tarman; Hedi Indra Januar; Muhammad Nursid
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 3 (2016): December 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i3.269

Abstract

Fatty acids and carotenoid has been known as an anticancer agent on both preventing and treating cancer disease. This study was conducted to analyze the fatty acid profile, carotenoid and in vitro anticancer activity of 12 sea cucumber harvested from Karimunjawa and Lampung waters. The aim of the study was to determin the potency of sea cucumbers as raw material for nutraceutical products. Fatty acid profile and carotenoid content were characterized by gas chromatography and spectrophotometry techniques, while in vitro anticancer activity was assessed by MTT assay against cervix (HeLa), breast (T47D and MCF-7) and colon (WiDR) cancer cells. Results of the study showed polyunsaturated fatty acid (PUFA) dominated the composition of fatty acids in the samples from both locations. Holothuria sp. was detected to contain the highest amount of carotenoid. Furthermore, the highest in vitro anticancer activity was detected also in the sample of Holothuria sp. The activity of 30 ppm Holothuria sp. extract against HeLa cell was detected to be almost equal to the 5 ppm doxorubicin control. Concentration of 5 ppm Holothuria sp. extract also showed positive result in killing 50% of MCF-7 and T47D, but capable to 100% kill HeLa and WiDR cells. At concentration of 25 ppm, the extract was able to kill all the 4 cells tested. Statistical analysis showed the amount of carotenoid and two particular fatty acid compounds (docosadienoic and eicosapentaenoic acid) significantly (P0.05) contributed to the cytotoxic activity that was found in the sea cucumber samples. Those compounds were found in highest concentration from Holothuria sp harvested from Lampung waters, thus being the most prospective raw material for nutraceutical or functional food ingredient with anticancer potency.
Co-Authors Ekowati Chasanah Hedi Indra Januar Iriani Setyaningsih Muhammad Nursid Nabila Ukhty Nurjanah Nurjanah R. Marwita Sari Putri Yusro Nuri Fawzya