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All Journal AGRITROP e-Journal Pustaka Kesehatan Berkala Ilmiah Pertanian UNEJ e-Proceeding Panrita Abdi - Jurnal Pengabdian pada Masyarakat Agriprima : Journal of Applied Agricultural Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis BIOVALENTIA: Biological Research Journal
TRI HANDOYO
Fakultas Pertanian Universitas Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Other

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PERUBAHAN KANDUNGAN ANTIOKSIDAN, POLIFENOL DAN PROFIL PROTEIN SELAMA PRA-PERKECAMBAHA PADA BIJI KAKAO Ulfaniah, Kiki; Handoyo, Tri; Sakdiyah, Zahratus
Berkala Ilmiah Pertanian Vol 1, No 3: FEBRUARI
Publisher : Berkala Ilmiah Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.406 KB)

Abstract

[ENGLISH] Cocoa proven as food source that rich in bioactive compounds, especially polyphenols that have a role as an antioxidant because it can stop the free radical reactions. As the change in lifestyle of the people and the development of technology, various ways have been made to improve the nutrition quality of the cocoa beans with pre-germination methods. This research aimed to study the effect of pre-germination methods that change the content of antioxidants, polyphenols and protein profiles of cocoa beans. The research was conducted in the Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, University of Jember, held from January 28 until April 30, 2013. Metodology research used Sulawesi cocoa beans type 1 beans without skin and with the seed coat, which is done with a completely randomized design (CRD) in the old pre-germination 0, 1, 2, 3, 4, 5 days and repeated 4 times. The results showed that pre-germination methods provide a very real effect on the polyphenol content and antioxidant cocoa beans without without and the seed coat. The highest antioxidant content of cocoa beans without the skin of 0.47 µg/mg at the old pre-germination for 3 days and cocoa beans with the seed coat by 0.41 µg/mg in the old pre-germination 5 days. Polyphenol content of cocoa beans without skin and with the best seed coat that is in the control treatment had the highest content of 2.67 µg/mg for cocoa beans without skin and 2.46 µg/mg for cocoa beans with the seed coat. Keywords: Cocoa; Polyphenols; Antioxidants; Free Radicals; Pre-Germination [INDONESIAN] Kakao terbukti sebagai sumber makanan kaya senyawa bioaktif terutama polifenol yang mempunyai kasiat sebagai antioksidan karena mampu menghentikan reaksi radikal bebas. Seiring perubahan pola hidup masyarakat dan berkembangnya teknologi, berbagai cara telah dilakukan untuk meningkatkan kualitas nutrisi pada biji kakao dengan metode pra-perkecambahan. Penelitian ini bertujuan untuk mengetahui pengaruh metode pra-perkecambahan terhadap perubahan kandungan antioksidan, polifenol dan profil protein biji kakao. Penelitian dilaksanakan di Laboratorium Genetika dan Pemuliaan Tanaman, Fakultas Pertanian, Universitas Jember, dilaksanakan mulai tanggal 28 Januari sampai 30 April 2013. Metode penelitian menggunakan biji kakao jenis Sulawesi 1 tanpa kulit dan dengan kulit biji, yang dilakukan dengan Rancangan Acak Lengkap (RAL) pada lama pra-perkecambahan 0, 1, 2, 3, 4, 5 hari dan diulang sebanyak 4 kali. Hasil penelitian menunjukan bahwa metode pra-perkecambahan memberikan pengaruh sangat nyata terhadap kandungan polifenol dan antioksidan biji kakao tanpa kulit dan dengan kulit biji. Kandungan antioksidan tertinggi untuk biji kakao tanpa kulit biji sebesar 0,47 µg/mg pada lama pra-perkecambahan 3 hari dan untuk biji kakao dengan kulit biji sebesar 0,41 µg/mg pada lama pra-perkecambahan 5 hari. Kandungan polifenol biji kakao tanpa kulit dan dengan kulit biji terbaik yaitu pada perlakuan kontrol memiliki kandungan tertinggi sebesar 2,67 µg/mg untuk biji kakao tanpa kulit dan 2,46 µg/mg untuk biji kakao dengan kulit biji. Kata kunci: Kakao; Polifenol; Antioksidan; Radikal Bebas; Pra-Perkecambahan How to citate: Ulfaniah K, T Handoyo, Z Sakdiyah. 2014. Perubahan kandungan antioksidan, polifenol dan profil protein selama pra-perkecambaha pada biji kakao. Berkala Ilmiah Pertanian 1(3): 43-46.
Cloning coat protein gene of CBSD (cassava brown streak disease) at cassava (Manihotesculentum) Restanto, Didik Pudji; ., Slameto; Kriswanto, Budi; Addy, Hardian Susilo; Handoyo, Tri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Cassava Brown Streak Disease (CBSD) is a major disease in cassava plants which have the serious problems in cassava plantations in the world, especially in Africa, Tanzania and India (Wassawaet., al, 2010). In Indonesia, the virus is still not optimal yet in the handling. The disease is present in plants that can destructive cassava leaves, stems and tubers.  It was greatly reduces the quality and production in the world such as India.  The decrease of cassava yield can reach 100% due to disease of CBSD (Lopez, 2003). The primer was designed from the coat protein gene of CBSD with a distance of 380 bp (Abarshiet.,al, 2012). The primers designed the forward primer (GGARCCRATGTAYAAATTTGC) and Reverse (GCWGCTTTTA  TYACAAAMGC). The RNA isolation have been used Plant Virus RNA Kit (Geneaid).  The CBSD RNA concentration around 55,2ng/ul.  The RT PCR program were one cycle of RT PCR reaction (45oC for 30 min), denaturation (45oC for 5 min) and 30 cycles for denaturation (94oC for 1 min), annealing (52oC for 30 sec), extention  (72oC for 1 min).  The results showed a single band of about 380 bp which is the  distance between the two primers were tested.  The multiplication shoot around 5 shoots per meristem explants with a combination of 0.5 ppm and 0.1 ppm BAP GA3 Keyword : Cassava Brown Streak Disease (CBSD), CASSAVA (Manihotesculentum), RT PCR
PENGARUH AMMONIUM (NH4+) DAN NITRAT (NO3-) TERHADAP PERTUMBUHAN DAN KANDUNGAN MINYAK ATSIRI TANAMAN KEMANGI (Ocimum basilicum) DENGAN SISTEM HIDROPONIK Damayanti, Dwi Putri Oktavia; Handoyo, Tri; S, Slameto
AGRITROP Vol 16, No 1 (2018): Agritrop : Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (871.653 KB) | DOI: 10.32528/agr.v16i1.1560

Abstract

Kemangi (Ocimum basilicum) merupakan salah satu sayuran dan menjadi bagian dari bahan pangan yang cukup disukai masyarakat. Tanaman kemangi di dalamnya juga mengandung minyak atsiri. Mengingat peran penting kemangi terhadap kehidupan manusia, maka diperlukan optimalisasi budidaya kemangi dengan hidroponik. Kandungan atsiri sendiri diketahui dapat diperoleh dari ekstrak daun kemangi, sehingga diperlukan daun kemangi yang berukuran lebar agar mampu menghasilkan minyak atsiri yang banyak. Berdasarkan fakta-fakta tersebut, oleh karena itu diperlukan penambahan NH4+ dan NO3- untuk merangsang pertumbuhan tanaman dan pembesaran sel. Penelitian ini dilaksanakan di Greenhouse dan Laboratorium CDAST Universitas Jember pada Mei hingga Agustus 2017. Metode penelitian yang digunakan ialah metode rancangan acak lengkap (RAL) faktorial yang terdiri dari 2 faktor perlakuan. Faktor pertama ialah beberapa konsentrasi nutrisi ammonium (NH4+) yang terdiri dari kontrol, 3 x 10-3 mol/l, 6 x 10-3 mol/l .Faktor kedua yakni faktor konsentrasi nutrisi nitrat (NO3-) yang terdiri dari kontrol, 3 x 10-3 mol/l, dan 6 x 10-3 mol/l. Hasil penelitian menunjukkan bahwa penambahan NH4+ dan NO3- berpengaruh terhadap pertumbuhan dan kandungan minyak atsiri kemangi. Konsentrasi NH4+  (3 x 10-3 mol/l) dan NO3- + (3 x 10-3 mol/l) menghasilkan minyak atsiri terbanyak yakni 0,050 ml
Peranan Sucrose Phosphate Synthase (SPS) dan Acid Invertase (AI) Internoda Tebu (Saccharum officinarum L.) dalam Akumulasi Sukrosa -, MISWAR; SUGIHARTO, BAMBANG; HANDOYO, TRI; AYU MADE, SRI
AGRITROP Vol. 26, No. 4 Desember 2007
Publisher : AGRITROP

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Abstract

Sucrose content is an important component of yield in sugarcane (Saccharum officinarum L.) juice and itssynthesis is determines by the activity of Sucrose phosphate synthase (SPS; EC. 2.4.1.14). This enzyme catalyzes thepenultimate reaction of the formation of sucrose from fructose-6-phosphate (F6P) and uridine-5-diphospho glucose(UDPG). Sucrose is synthesized on leaf and translocated into stalk at where it is accumulated to be accumulatedit. In contrast to SPS, acid invertase (AI) is an enzyme that hydrolyze sucrose to glucose and fructose. The abilityof sugarcane to accumulate the sucrose in stalk is determined by the differences between SPS and AI activities.This study was aimed know role of the internodes SPS and AI in sucrose accumulation in the internodes of somesugarcane varieties. SPS, AI and sucrose were extracted from internodes 1, 3 and 5 of 9 month old sugarcane. Theresults showed that sucrose content for all varieties increased concomitant with the rise in the age of internodes.The decrease in AI activity in internode caused the increase in sucrose content in internodes. Meanwhile, highersucrose content in the older internodes was not followed by higher in SPS activity. Generally, there was strongnegative correlation between sucrose content of internodes with SPS and AI activity. In conclusion, the ability ofsugarcane to accumulate sucrose at internodes was primary determined by AI activity, while SPS activity had asmall role on sucrose accumulation at internodes.
DIFFERENT NITRATE AND AMMONIUM LEVELS MEDIA ON CHANGES OF NITROGEN ASSIMILATION ENZYMES IN RICE Handoyo, Tri; Ilman Widuri, Laily; Pudji Restanto, Didik
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.901 KB) | DOI: 10.24233/biov.7.1.2021.204

Abstract

Nitrogen (N) is an important nutrient for the growth and development of rice plants, required in large quantity and often limiting factor of rice yields. The research was to understand the different sources and levels of nitrogen in rice plant on the activity of N assimilation enzymes, including nitrate reductase (NR), glutamine synthase (GS) content, glutamate synthase (Gogat) content, content, ammonium (NH4+) and nitrate (NO3-) content on the leaves. Paddy (Ciherang variety) was grown in sand media containing Hoagland solution with different sources (ammonium and nitrate) and levels (0.4, 0.8, 1.6, 3.2, 6.4, and 12.8 mM) of nitrogen. Nitrogen assimilation was observed from leaves at one month of age. The NR activity increased on both Nitrogen sources, it was a higher activity in media contained nitrate. Also, the activity of GS showed higher in media contains nitrate, but its activity was decreased after application 1.6 mM of nitrate and 3.2 mM of ammonium. Western blot analysis of GS1 and GS2 showed that the band pattern of protein was similar to these enzyme activities. Nitrate content in leaves gradually increased in both sources of nitrogen and higher than 3.2 mM ammonium application caused an increase in ammonium content in leaves, but the nitrate content decreased. This research resulted that the available source of N for rice was in nitrate form, easily by the rice plants during the growth stage.
Uji Aktivitas Protein Larut Air Umbi Porang (Amorphophallus muelleri Blume) terhadap Escherichia coli dan Staphylococcus aureus Putu Gita Maya Widyaswari Mahayasih; Tri Handoyo; Mochammad Amrun Hidayat
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

The objective of this study was to determine antibacterial activity of water soluble protein of Porang (Amorphalus muelleri Blume) tuber. The water soluble protein extract was tested against both Eschericia coli and Staphylococcus aureus using filter paper disk method. The inhibition zone of bacterial growth was calculated for both of bacterial cultures. It was found that water soluble protein exhibited antibacterial activity at linear range 0.3-1.9 μg and 0.1-1.9 μg for E. coli and S. aureus respectively, sugesting that S. aureuswas more susceptible than E. coli to this protein. From the electrophoretic profile using 12.5% sodium dodecyl sulfate (SDS) and comassie blue staining (CBS), two protein bands were observed with molecular weight 17 and 19 kDa respectively. The water soluble protein were further evaluated using periodic acid staining (PAS). Pink band with molecular weight 250 kDa and > 250 kDa was found in PAS profile, sugesting the availability of carbohydrate molecule in water soluble protein extract of porang tubers.
PERUBAHAN KANDUNGAN ANTIOKSIDAN, POLIFENOL DAN PROFIL PROTEIN SELAMA PRA-PERKECAMBAHA PADA BIJI KAKAO Kiki Ulfaniah; Tri Handoyo; Zahratus Sakdiyah
Berkala Ilmiah Pertanian Vol 1 No 3 (2014): FEBRUARI
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.406 KB)

Abstract

[ENGLISH] Cocoa proven as food source that rich in bioactive compounds, especially polyphenols that have a role as an antioxidant because it can stop the free radical reactions. As the change in lifestyle of the people and the development of technology, various ways have been made to improve the nutrition quality of the cocoa beans with pre-germination methods. This research aimed to study the effect of pre-germination methods that change the content of antioxidants, polyphenols and protein profiles of cocoa beans. The research was conducted in the Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, University of Jember, held from January 28 until April 30, 2013. Metodology research used Sulawesi cocoa beans type 1 beans without skin and with the seed coat, which is done with a completely randomized design (CRD) in the old pre-germination 0, 1, 2, 3, 4, 5 days and repeated 4 times. The results showed that pre-germination methods provide a very real effect on the polyphenol content and antioxidant cocoa beans without without and the seed coat. The highest antioxidant content of cocoa beans without the skin of 0.47 µg/mg at the old pre-germination for 3 days and cocoa beans with the seed coat by 0.41 µg/mg in the old pre-germination 5 days. Polyphenol content of cocoa beans without skin and with the best seed coat that is in the control treatment had the highest content of 2.67 µg/mg for cocoa beans without skin and 2.46 µg/mg for cocoa beans with the seed coat. Keywords: Cocoa; Polyphenols; Antioxidants; Free Radicals; Pre-Germination [INDONESIAN] Kakao terbukti sebagai sumber makanan kaya senyawa bioaktif terutama polifenol yang mempunyai kasiat sebagai antioksidan karena mampu menghentikan reaksi radikal bebas. Seiring perubahan pola hidup masyarakat dan berkembangnya teknologi, berbagai cara telah dilakukan untuk meningkatkan kualitas nutrisi pada biji kakao dengan metode pra-perkecambahan. Penelitian ini bertujuan untuk mengetahui pengaruh metode pra-perkecambahan terhadap perubahan kandungan antioksidan, polifenol dan profil protein biji kakao. Penelitian dilaksanakan di Laboratorium Genetika dan Pemuliaan Tanaman, Fakultas Pertanian, Universitas Jember, dilaksanakan mulai tanggal 28 Januari sampai 30 April 2013. Metode penelitian menggunakan biji kakao jenis Sulawesi 1 tanpa kulit dan dengan kulit biji, yang dilakukan dengan Rancangan Acak Lengkap (RAL) pada lama pra-perkecambahan 0, 1, 2, 3, 4, 5 hari dan diulang sebanyak 4 kali. Hasil penelitian menunjukan bahwa metode pra-perkecambahan memberikan pengaruh sangat nyata terhadap kandungan polifenol dan antioksidan biji kakao tanpa kulit dan dengan kulit biji. Kandungan antioksidan tertinggi untuk biji kakao tanpa kulit biji sebesar 0,47 µg/mg pada lama pra-perkecambahan 3 hari dan untuk biji kakao dengan kulit biji sebesar 0,41 µg/mg pada lama pra-perkecambahan 5 hari. Kandungan polifenol biji kakao tanpa kulit dan dengan kulit biji terbaik yaitu pada perlakuan kontrol memiliki kandungan tertinggi sebesar 2,67 µg/mg untuk biji kakao tanpa kulit dan 2,46 µg/mg untuk biji kakao dengan kulit biji. Kata kunci: Kakao; Polifenol; Antioksidan; Radikal Bebas; Pra-Perkecambahan How to citate: Ulfaniah K, T Handoyo, Z Sakdiyah. 2014. Perubahan kandungan antioksidan, polifenol dan profil protein selama pra-perkecambaha pada biji kakao. Berkala Ilmiah Pertanian 1(3): 43-46.
IDENTIFIKASI KARAKTERISTIK MORFOLOGI DAN MOLEKULER 21 VARIETAS PADI AROMATIK (Oryza sativa L.) BERDASARKAN PENANDA RAPD (Random Amplified Polymorphic DNA) Irza Guari Syah Fitri; Tri Handoyo
Berkala Ilmiah Pertanian Vol 2 No 2 (2019): MEI
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (804.3 KB) | DOI: 10.19184/bip.v2i2.16174

Abstract

ABSTRACT Aromatic rice (Oryza sativa L.) is one type of rice that has aroma and high selling price than non-aromatic rice. The variety development is needed to form superior aromatic rice. The variety development requires information about kinship and diversity between varieties. Kinship analysis with identification of morphological characteristics is an observation based on taxonomic approach. This research aims to determine the morphological characteristics and kinship of 21 aromatic rice varieties. The research was conducted from March to September 2018 with a randomized block design (RBD) of 3 replications. Observation variables used plant height (cm), number of productive tillers (tiller), age of flowering and harvesting (days), weight of 100 grain (gram), shape of grain, color of grain, color of rice and DNA band pattern as molecular data. Morphological data were analyzed by variance (ANOVA) and cluster analysis with UPGMA program (Unweight Pair Group Method with Arithmetic Mean) using DIST Coefficient on NTSYS 2.02 software. Molecular data showed in visualisation of DNA band using Primer OPB-07 based on RAPD marker. The research results showed the differences in morphological characters and kinship levels. Cluster analysis forms 2 clusters and 2 sub-clusters. Keywords: Aromatic Rice, Mophological Diversity, RAPD, Dendrogram ABSTRAK Padi aromatik (Oryza sativa L.) merupakan salah satu jenis padi yang memiliki aroma wangi dan memiliki harga jual yang lebih tinggi dibanding padi biasa. Pengembangan varietas diperlukan untuk membentuk padi aromatik yang lebih unggul. Pengembangan varietas membutuhkan informasi tentang kekerabatan dan keanekaragaman antar varietas. Analisis kekerabatan dengan identifikasi karakteristik morfologi merupakan suatu observasi berdasarkan pendekatan taksonomi. Percobaan ini bertujuan untuk mengetahui karakteristik morfologi dan molekuler 21 varietas padi aromatik. Percobaan dilakukan di mulai Maret - September 2018 dengan Rancangan Acak Kelompok (RAK) 3 ulangan. Variabel pengamatan menggunakan tinggi tanaman (cm), jumlah anakan produktif (batang), umur berbunga dan panen (HSS), bobot 100 gabah, bentuk gabah, warna gabah, dan warna beras serta pola pita DNA sebagai data molekuler. Data morfologi dianalisis dengan ragam (ANOVA) serta dianalisis gerombol dengan program UPGMA (Unweight Pair Group Method with Arithmetic Mean) menggunakan DIST Koefisien pada software NTSYS 2.02. Data molekuler ditunjukkan dalam bentuk visualisasi DNA menggunakan primer OPB-07 berdasarkan penanda RAPD. Hasil percobaan menunjukkan perbedaan karakter morfologi dan tingkat kekerabatan yang berbeda. Analisis gerombol membentuk 2 cluster dan 2 sub-cluster. Kata kunci: Padi Aromatik, Kekerabatan Morfologi, RAPD, Dendrogram
KOLAM GIZI AKUAPONIK UNTUK KETAHANAN PANGAN MASYARAKAT URBAN KELURAHAN KARANGREJO KABUPATEN JEMBER DI MASA PANDEMI COVID-19 Tri Handoyo; Mahros Darsin; Laily Ilman Widuri
Panrita Abdi - Jurnal Pengabdian pada Masyarakat Vol. 6 No. 1 (2022): Jurnal Panrita Abdi - Januari 2022
Publisher : LP2M Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/pa.v6i1.12445

Abstract

Limited knowledge of society about cultivation activities in the urban area leads to low practical urban farming activities during the pandemic. This study aimed to develop a productive urban community to increase family food security and apply science and technology through aquaponic ponds to optimize small areas around their house. This community service activity was conducted in the Karangrejo subdistrict, Sumbersari district, Jember Regency, East Java Indonesia, on September – November 2020. Three methods were applied during this activity, including counseling for food security during pandemics, training for aquaponic techniques, and accompaniment during the harvesting period. Success indicators of this program showed by the increment of the community's knowledge and skill about food security, aquaponic, and harvesting techniques. Through assistance to the community from the beginning to the end, we expected that this nutrition aquaponic pond program could be a provision to motivate people for continuing productive activities during the pandemic. This community service activity could optimize limited land around the house as nutrition sources for urban communities. The community's participation and contribution to maintaining the aquaponic pond and cultivation activity become a key for the sustainable cultivation cycle of aquaponics products. Society could improve the skill and knowledge for urban communities to create autonomy metropolitan areas during pandemic Covid-19. --- Kerterbatasan kapasitas pengetahuan masyarakat tentang teknik bercocok tanam, menyebabkan banyak masyarakat belum maksimal menerapkan aktivitas urban farming di masa pandemi.  Tujuan diadakannya kegiatan pengabdian ini adalah untuk menciptakan masyarakat urban yang produktif dan mandiri dalam menjaga ketahanan pangan dan gizi keluarga, dan menerapkan iptek praktek budidaya tanaman dan ikan air tawar secara akuaponik sebagai bentuk optimalisasi lahan sempit di sekitar rumah. Kegiatan pengabdian masyarakat dilaksanakan di lahan milik salah satu anggota komunitas ibu-ibu kelompok pengajian yang berlokasi di Lingkungan Pelindu Barat, Kecamatan Sumbersari, Kabupaten Jember, Jawa Timur pada bulan September-November 2020. Metode pengabdian dilakukan  dengan tiga tahapan yakni penyuluhan tentang ketahanan pangan keluarga di masa pandemi Covid-19, pelatihan teknik budidaya akuaponik dan pendampingan kegiatan panen produk akuapoik. Indikator keberhasilan kegiatan ini yakni peningkatan aktivitas produktif untuk memenuhi kebutuhan gizi keluarga, peningkatan keterampilan tentang teknik budidaya akuaponik, dan peningkatan pengetahuan teknik panen sayuran dan ikan lele dari hasil akuaponik. Hasil kegiatan menunjukkan bahwa kolam gizi aquponik dapat menjadi salah satu solusi yang dapat diberikan untuk mengoptimalkan pemanfaatan lahan sempit sekaligus menjadi sumber gizi masyarakat di kawasan urban. Selain mendapatkan tambahan keilmuan, masyarakat juga dimotivasi untuk ikut aktif berpartisipasi dalam pemeliharaan dan kegiatan bercocok tanam, dengan harapan bahwa keberlanjutan siklus budidaya ikan dan sayur dapat terus berkelanjutan. Bekal pengetahuan dan skill yang diberikan pada kegiatan pengabdian ini diharapkan dapat dikembangkan oleh  masyarakat untuk menjadi kawasan urban mandiri di tengah situasi pandemi.
Pengaruh Dosis dan Waktu Aplikasi Pupuk KCl Terhadap Pertumbuhan dan Hasil Tanaman Buncis (Phaseolus vulgaris L.) Muhammad Nur Thoriq Alfy; Tri Handoyo
Agriprima : Journal of Applied Agricultural Sciences Vol 6 No 1 (2022): MARCH
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v6i1.431

Abstract

Buncis (Phaseolus vulgaris L.) merupakan tanaman legum yang memiliki kandungan gizi cukup tinggi. Produksi buncis belum mampu memenuhi permintaan buncis yang tinggi. Rendahnya produksi buncis dapat disebabkan oleh ketersediaan unsur hara. Kebutuhan unsur hara kalium (K) pada buncis tidak tercukupi karena kalium bersifat mudah tercuci. Upaya pemenuhan unsur hara kalium dilakukan melalui pemupukan KCl. Dosis pupuk yang tepat dapat membantu tanaman mencukupi kebutuhannya untuk tumbuh optimal. Waktu aplikasi pupuk yang tepat membantu tanaman menjaga ketersediaannya akan unsur hara. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian dosis pupuk KCl dan waktu aplikasi pupuk KCl terhadap pertumbuhan dan hasil tanaman buncis (Phaseolus vulgaris L.) menggunakan rancangan acak lengkap (RAL) faktorial yang terdiri dari 2 faktor perlakuan dan 3 ulangan. Faktor pertama yaitu dosis pupuk KCl K0 = 0 g/tanaman, K1 = 2,5 g/tanaman, K2 = 3 g/tanaman, K3 = 3,5 g/tanaman. Faktor kedua yaitu waktu aplikasi pupuk KCl W1 = 14 hst, W2 = 14 hst dan 21 hst, W3 = 14 hst, 21 hst, 28 hst. Data yang diperoleh selanjutnya dianalisis dan diuji menggunakan Uji Lanjut Jarak Berganda Duncan pada taraf 5%. Hasil penelitian menunjukkan bahwa hasil tertinggi diperoleh perlakuan K3 dengan dosis pupuk KCl 3,5 g/tanaman dan perlakuan W3 dengan waktu aplikasi pupuk KCl pada 14 hst, 21 hst, dan 28 hst.
Co-Authors Ahdatu Uli Khikamil Maulidiya Bambang Sugiharto Bambang Sugiharto Budi Kriswanto, Budi Damayanti, Dwi Putri Oktavia Dwi Putri Oktavia Damayanti Dwika Nano Hariyanto Fariza Oktaviani HARDIAN SUSILO ADDY Irma Novita Sari Irza Guari Syah Fitri Kacung Hariyono Kiki Ulfaniah Kiki Ulfaniah Laily Ilman Widuri Laily Ilman Widuri, Laily Ilman Mahros Darsin Marcus dos Santos Soares MISWAR - Mochammad Amrun Hidayat Mohammad Ubaidillah Muhammad Nur Thoriq Alfy Muhammad Syauqi Parawita Dewanti Pudji Restanto, Didik Putu Gita Maya Widyaswari Mahayasih Restanto, Didik Pudji Slameto Slameto SRI AYU MADE Suryo Prakoso, Suryo Tri Agus Siswoyo Veronenci Yuliarbi Farlisa Zahratus Sakdiyah Zahratus Sakdiyah Zaiyin Rizky Ageng Maulidia