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Isolasi dan Identifikasi Bakteri Gram Negatif pada Uterus Sapi Aceh yang Mengalami Repeat Breeding Indi Rafika; Cut Nila Thasmi; Herrialfian Herrialfian; Rosmaidar Rosmaidar; Hafizuddin Hafizuddin
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.17017

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui jenis bakteri Gram negatif pada uterus sapi Aceh yang mengalami kawin berulang. Dalam penelitian ini digunakan enam ekor sapi Aceh betina yang terdiri atas tiga ekor sapi fertil (K1) dan tiga ekor yang mengalami kawin berulang (K2, repeat breeding, RB). Pengambilan sampel bakteri dalam uterus sapi dilakukan dengan menggunakan swab steril yang disimpan dalam media nutrient broth (NB) untuk isolasi dan identifikasi jenis bakterinya. Berdasarkan hasil penelitian, baik K1 dan K2, positif terinfeksi bakteri dan diperoleh 16 isolat bakteri di antaranya enam isolat berasal dari K1 dan sepuluh isolat berasal dari K2. Dari 10 isolat bakteri Gram negatif yang ditemukan pada sapi RB terdiri atas Escherichia coli (E. coli) (30,00%), Pseudomonas sp. (30,00%), Enterobacter sp. (20,00%), Klebsiella sp. (20,00%). Disimpulkan bahwa bakteri yang paling banyak menginfeksi uterus sapi RB adalah E. coli dan Pseudomonas sp. (Isolation and identification of gram negative bacteria in uterine Aceh cow with repeat breeding) ABSTRACT. The study determined the type of Gram negative bacteria of the Aceh cattle uterus which has been diagnosed with repeat breeding. The study was conducted on six Aceh cows consists of 3 normal fertile cows (K1) and 3 repeat breeding cows (K2, RB). The bacteria in uterine samples were collected using sterile swab, kept into nutrient broth (NB) for further isolation and identification of bacteria. The result of the study showed that the total of 16 isolates of bacteria was observed in all cows : 6 isolates from group K1 and 10 isolates from group K2. A total of 10 isolates from cattle with repeat breeding consist of Escherichia coli (E. coli) (30.00%), Pseudomonas sp (30.00%), Enterobacter sp (20.0%), and Klebsiella sp (20.0%). It can be concluded that the most bacteria that infect uterine cows with repeat breeding are E. coli and Pseudomonas sp.
1. The Sensitivity Level Of Gentamicine, Cholramphenicol and Penicillin Inhibiting The Growth Of Pseudomonas Aeruginosa Bacteria Isolate From Aceh Bull Prepunce Mada Admi; Annisa Anwar Sitorus; Rinidar Rinidar; Amalia Sutriana; Rosmaidar Rosmaidar; Sugito Sugito
Jurnal Medika Veterinaria Vol 15, No 1 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i1.20856

Abstract

This study aims to determine the sensitivity level of gentamicin, chloramphenicol and penicillin antibiotics to inhibit the bacterial growth of P. aeruginosa taken from preputium isolate of Aceh cattle. This Stock of Pseudomonas aeruginosa isolate from Microbiology Laboratory of FKH USK, re-identified its purity through: indole test, methyl red test, sugar test and as well as on Nutrient Broth (NB) media; incubated at 37ºC for 24 hours; observed the turbidity level of 0.5 Mc Farland solution. Then, the antibiotic sensitivity test was carried out using the method of Mueller Hinton Agar (MHA). The observations were made by measure the inhibition zone using the calipers in millimeters (mm) against antibiotics. The results of the observation of the inhibition zone on the gentamicin antibiotic was averaged of 25.5 mm, 23.7 mm chloramphenicol, and 12.1 mm penicillin. This study concluded that the gentamicin, chloramphenicol, and penicillin antibiotic were still effective against P. aeruginosa bacteria with the highest level of sensitivity seen in gentamicin antibiotic. 
The Effect of Vitamin C Addition in Andromed® Dilution on Procentage of Motility and Spermatozoa Intact Plasma Membrane of Aceh Bull After Freezing Hasan Al Aslam; Dasrul Dasrul; Rosmaidar Rosmaidar
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.488 KB) | DOI: 10.21157/j.med.vet..v8i1.3326

Abstract

This study aimed to determine the effect of vitamin C addition in andromed® dilution on procentage of motility and intact plasma membrane post thawing of aceh bull spermatozoa. This research used fresh semen aceh bull were collected using artificial vagina and divided into four treatments.Control treatment (P0) semen without the addition of vitamin C, first treatment (P1) semen plus vitamin C 0.5 g/100 ml of dilution, second treatment (P2) semen plus vitamin C 1.0 g/100 ml of dilution, third treatment (P3) semen plus vitamin C 2.0 g/100 ml of dilution. The experiment design used was completely randomized design (CRD). Data was analyzed using ANOVA (Analysis of Variant) and followed by Duncan’s multiple range (Duncan’s Multiple Range Test) 5% confidence interval. The result showed that the addition of vitamin C influence the motility and intact plasma membrane percentage of aceh bull spermatozoa after thawing. The addition of vitamin C 0.5 g/100 ml and 1.0 g/100 ml increase motility and intact plasma membrane percentage, while vitamin C 2.0 g/100 ml decrease motility and intact membrane plasma percentage after thawing.Key words: intact plasma membrane, motility, aceh bull, spermatozoa, vitamin C
PENGARUH UMUR TERHADAP PENJANTANAN LARVA IKAN LELE DUMBO (Clarias gariepinus) YANG DIRENDAM MENGGUNAKAN HORMON METIL TESTOTERON ALAMI (The Influence of Age on Masculinization of Clarias gariepinus Larvae Immersed by Methyl Testosterone Natural Hormone) Hattanul Mulia; Rosmaidar Rosmaidar; Dasrul Dasrul; Dwinna Aliza; Dian Masyitha; Sugito Sugito
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.017 KB) | DOI: 10.21157/j.med.vet..v10i1.4036

Abstract

This research aims to examines the influence of age on masculinization of Clarias gariepinus larvae immersed by methyl testosterone (MT) natural hormone. This research used 270 catfish larvae divided into 3 groups of age those are 5, 10, and 20 days. The research implemented complete random design (CRD). Immersion was performed using methyl testosterone hormone at the dose of 0.25 g/L for 20 hours. Observation of fish was done after 60 day-old fish. Data obtained was analyzed using one-way analysis of variance The average percentages of male-sex catfish after immersing in natural MT on group I, II, and III were 97.67, 91.07, and 86.67%, respectively. The results showed that the age of the larvae did not affect significantly (P0.05) on the percentage of male sex of Clarias gariepinus. The average percentage of male sex in 5 days age group was higher than 10 and 20 days age group. In conclusion, the masculinization of Clarias gariepinus larva immersed by MT natural hormone is not influenced by age of fish before dipping.
The Effect of Dipping Time in Natural Methyl Testosterone Hormone on Male Forming Succesiveness Process of Tilapia (Oreochromis niloticus) Larvae Rosmaidar Rosmaidar; dwinna aliza; Jesica Ramadhanita
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.041 KB) | DOI: 10.21157/j.med.vet..v8i2.3361

Abstract

The purpose of this research was to know the influence of dipping time in natural methyl testosterone hormone (MT) on successful male forming process of tilapia (Oreochromis niloticus) larvae. This research used 360 7 days old tilapia larvae, purchased from cultivation of brackish water, Ujung Batee, Banda Aceh. The samples were divided into 4 treatment groups with three repetitions, they were P0 (control group without treatment), PII (Tilapia larvae were dipped in natural MT for 10 hours), PII (Tilapia larvae were dipped in natural MT for 20 hours), and PIII (Tilapia larvae were dipped in natural MT for 30 hours). All tilapia larvae were maintained until the age of 60 days, before the sex of the fish examined. The average percentage of male tilapia in group P0, PI, PII, and PIII were 50.28, 87.77, 90.06, and 95.05%, respectively. Statistical analysis showed that there were significant differences (P0.05) among P0, PI, PII, and PIII. Based on result of this research, it can be concluded that the longest dipping time the highest the percentage of male tilapia.Key words: larvae, tilapia, natural hormone methyl testosterone, the male sex percentage
14. PENGARUH LAMA PERENDAMAN LARVA DALAM HORMON METIL TESTOSTERON ALAMI TERHADAP PENJANTANAN IKAN LELE DUMBO (Clarias gariepinus) The Effect of Soaking Time of African Catfish Larvae (Clarias gariepinus) in Natural Methyl Testosterone Hormone on Masculinization Rosmaidar R
Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.78 KB) | DOI: 10.21157/j.med.vet..v10i2.4629

Abstract

The purpose of this study was to determine the effect of immersion time on African catfish (Clarias gariepinus) larvae in natural methyl testosterone hormone on masculinization. This study was conducted in Center of Fish Culture Lamnyong, Banda Aceh. Experimental animals used in this study were 270 African catfish larvae aged 10 days. The study was designed using completely randomized design (CRD) with 3 treatments and 3 replications. African catfish larvae aged 10 days in group P1, P2, and P3 were immersed in natural methyl testosterone hormone for 10 hours, 20 hours, and 30 hours, respectively. Observations were carried out until fish reach 50 days old. Data were analyzed by analysis of variance (Anova) followed by least significant difference test (LSD). Percentage of male catfish observed in P1, P2, and P3 were 64.24 %, 93.11%, dan 59.94% respectively. As a conclusion, immersion with natural methyl testosterone hormone can change the sex of catfish to be male. ____________________________________________________________________________________________________________________ Key words: african catfish, methyl testosterone natural, masculinization
Masculinization of Tilapia (Oreochromis niloticus) Using Methyl Testosterone Natural Hormone Rosmaidar R; Winaruddin W; Mimi Herlina
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.362 KB) | DOI: 10.21157/j.med.vet..v8i2.3347

Abstract

This study aimed to determine the effect of methyl testosterone natural hormoneon the rate of masculinization of tilapia (Oreochromis niloticus). The study used 270 tilapia larvae with the age of 7 days as experimental animals. Tilapia larvae were divided into 3 treatments; eachtreatment consisted of 30 larvae fish. This study was an experimental study using completely randomized design with 3 treatments and 3replications. Treatment I as control group was fed with pellets ad libitum, treatment II was given feed containing methyl testosterone naturalhormone, and treatment group III was given hormone treatment by soaking the fish larvae with methyl testosterone natural hormone for 20hours. Larvae were raised in accordance with applicable procedures. Observation was conducted when the larvae fish were at 60 days old andthe sex of fish can be observed. Parameter examined was the percentage of the male sex. The average (± SD) percentage of male tilapia in groupPI, PII, and PIII were 36.39±3.97, 66.72±6.13, and 82.37±6.87%, respectively. The results showed that the percentage of male sex of tilapia fedwith natural hormone of methyl testosterone through the diet and dipping was significantly different (P0.05) from control fish. In conclusion,the administration of natural hormone of methyl testosterone increased the percentage of the male sex of tilapia).____________________________________________________________________________________________________________________Key words: tilapia larvae, natural hormone methyl testosterone, the male sex percentage
22. PENGARUH UMUR LARVA IKAN NILA (Oreochromis niloticus) TERHADAP PENINGKATAN PENJANTANAN MENGGUNAKAN HORMON METHYL TESTOSTERONE (MT) ALAMI Effect of Tilapia (Oreochromis niloticus) Age in Masculinization Process Using Methyl Testosterone (MT) Natural Hormones Rosmaidar R
Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.745 KB) | DOI: 10.21157/j.med.vet..v10i2.4621

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This research aims to know the influence of larvae of nile tilapia (Oreochromis niloticus) age on masculinization process by the administration of methyl testosterone (MT) natural hormones. This research used 270 nile tilapia larvae divided into 3 age of groups: 5 days (PI), 10 days (PII), and 20 days (PIII), with three repetitions. Larvae were dipped in MT natural hormone for 20 days then reared until age of 60 days, followed by observations of fish sex. The average percentage of male-sex of tilapia fish after dipping with methyl testosterone natural hormone on group PI, PII, and PIII were 68.07%, 66.47%, and 57.29%, respectively. It can be concluded that the age of larvae do not affect the percentage of masculinization process of nile tilapia fish. ____________________________________________________________________________________________________________________ Key words: larvae tilapia, masculinization, methyl testosterone natural hormone
Perbandingan Kinerja Berahi dan Level Estradiol Kambing Kacang dan Kambing Nubian yang Diinduksi dengan PGF2α Mulyadi Adam; Raja Nurul Huda; Widya Zahara; Tongku Niswan Siregar; Sri Wahyuni; Cut Nila Thasmi; Rosmaidar Rosmaidar
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (10432.789 KB) | DOI: 10.22146/jsv.38444

Abstract

The experiment was conducted to compare estrous performance between and estradiol level kacang and Nubian goats estrous induction by prostaglandin F2 alpha (PGF2α). Eight female goats were divided into two groups, groups 1 (K1) consisted five Nubian goats and group 2 (K2) included three kacang goats with criterias as follows: age 1.5-3.0 years old, not pregnant, at least two months postpartum, and healthy clinically. All goats were estrous synchronized by double injection 7,5 mg PGF2α intramuscularly with 10 days interval. Goats that appeared estrus symptoms were mated naturally to a male goat. Determination of estrous intensity was performed using scoring method. Blood samples were taken at the first time the goats were mated (day 0). The measurement of estradiol concentration was done by enzyme linked immunosorbent assay (ELISA) method. Data of intensity, onset, duration, and estradiol level were analyzed using t test. The results showed that the rate of intensity, onset, duration, and estradiol level of estrous at KI vs KII were 2.08±0.52 vs 1.85±0.67, 56.00±34.11 vs 40.80±18.20 hours, and 24.00±26.15 vs 33.80±9.10 hours, and 103.51±42.49 vs 67.95±38.44 pg/ml (P>0,05). It can be concluded there was no difference of estrous performance and estradiol level between kacang and Nubian which estrous induction with PGF2α. The relationship between the intensity of estrus estradiol level in the Nubian goats was 0.995 while the kacang goats was 0.890.
Pemberian Ekstrak Akar Pasak Bumi Meningkatkan Kualitas Spermatozoa Domba Waringin Amalia Sutriana; Mira Ayu Lestari Hasibuan; Nurhazimah Nurhazimah; Teuku Armansyah; Tongku Nizwan Siregar; Budianto Panjaitan; Hafizuddin Hafizuddin; Arman Sayuti; Dwinna Aliza; Rosmaidar Rosmaidar
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.503 KB) | DOI: 10.19087/jveteriner.2021.3.317

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak akar pasak bumi (Eurycoma longifolia Jack) terhadap peningkatan konsentrasi testosteron domba Waringin. Dalam penelitian ini digunakan dua ekor domba waringin jantan berumur ±4 tahun. Penelitian ini merupakan penelitian eksperimen dengan menggunakan rancangan pola bujur sangkar latn 2 x 2 sehingga hewan percobaan diberi perlakuan 20 mL akuades per oral sebagai kontrol (P1) dan 20 mL larutan yang mengandung 45 mg/kg bobot badan ekstrak pasak bumi yang diberikan per oral (P2). Perlakuan diberikan selama enam hari pada pukul 09.00 WIB. Pada bulan ke-1 domba pertama mendapat perlakuan P1 sedangkan domba kedua mendapat perlakuan P2. Sebaliknya, pada bulan ke-2 domba pertama mendapat perlakuan P2 sedangkan domba kedua mendapat perlakuan P1. Koleksi darah untuk pemeriksaan konsentrasi hormon testosteron dilakukan lima jam setelah pemberian ekstrak akar pasak bumi (Eurycoma longifolia Jack). Analisis konsentrasi testosteron dilakukan menggunakan metode enzyme linked immunosorbent assay (ELISA) yang diukur pada hari 1, 3 dan 6. Data yang diperoleh dianalisis dengan uji sidik ragam pola bujur sangkar latin. Hasil analisis statistika menunjukkan bahwa volume semen, motilitas spermatozoa, viabilitas spermatozoa dan abnormalitas spermatozoa pada domba yang diberikan ekstrak akar pasak bumi (E. longifolia Jack) menunjukkan perbedaan yang signifikan (P<0,05) dibandingkan dengan kontrol namun konsentrasi testosteron tidak menunjukkan perbedaan yang signifikan (P>0,05). Disimpulkan bahwa pemberian ekstrak akar pasak bumi meningkatkan kualitas spermatozoa namun tidak menyebabkan peningkatan konsentrasi hormon testosteron pada domba waringin.