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Deteksi Antibodi Serum Terhadap Virus Avian influenza pada Ayam Buras Darmawi Darmawi; Zakiyah Heryawati Manaf; Darniati Darniati; Fakhrurrazi Fakhrurrazi; Mahdi Abrar; Erina Erina
Jurnal Agripet Vol 12, No 1 (2012): Volume 12, No. 1, April 2012
Publisher : Agricultural Faculty

Detection on Serum Antibodies of Native Chickens to Avian influenza VirusABSTRACT. An important approach of controlling against Avian Influenza should be determined to detect the antibody titres of bird flu caused by Influenza virus H5N1 in Indonesia. The aim of the present study was to detect the antibodies to Avian Influenza in serum of native chickens. This study utilized 123 serum samples collected from the axilaris vein (left or right) of native chickens. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that indication of natural infection by Avian Influenza (H5N1) in native chickens, as shown that out of 123 serum samples, 16 (13,01%) were tested positive by HI, while only 10 (8,13%) were tested protective to Avian influenza infection. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus stimulated variety level of formation antibody titres in native chickens.

Efek Pemberian Ekstrak Jaloh Dikombinasi dengan Probiotik dan Kromium Terhadap Profil Hematologi dan Titer Antibodi Vaksin ND pada Ayam Broiler yang Mengalami Stres Panas Sugito Sugito; Fakhrurrazi Fakhrurrazi; M. Isa
Jurnal Agripet Vol 11, No 2 (2011): Volume 11, No. 2, Oktober 2011
Publisher : Agricultural Faculty

The effect of jaloh extract combined with probiotic and chromium on the haematology profiles and ND antibody vaccine of broiler given heat stressABSTRACT. The purpose of this study is to determine the respons of chicken broiler under heat stress that given drinking water contained extract jaloh combined with probiotic and Cr mineral in the profile of haematology and antibody respons of Newcastle Disease vaccine. Twenty four of 20-day old Cobb broiler chickens were randomly assigned and devided to 6 treatment groups. Completely randomized design was used in this study. The treatments were as follows: 1) chickens under heat stress, given jaloh extract, probiotic, and chromium (P1); 2. chickens under heat stress, given jaloh extract and probiotic (P2); 3. chickens under heat stress, given jaloh extract and chromium (P3); chickens under heat stress, given jaloh extract (P4); 5. chickens under heat stress without given jaloh extract nor chromium and probiotic (P5); and 6. chickens given no heat stress and jaloh extract nor chromium and probiotic (P6). Extract jaloh dose applied is 1.000 mg/lt water, dose of probiotic is 108 cfu/lt drinking water, and chromium given in the form of chloride chromium with dose 1000 ug/lt water. Local heat stress is provided by increasing cage temperature to the range of 33.0 ± 1°C during 5 hours per day u within 15 days. Jaloh extract, probiotic, and chromium treatments in drinking water were given at 2 hour before cage temperature reaching 33 ± 1oC and were stopped being given after 1 hour, when cage temperature back to room temperature. Blood was taken before chickens were ethanuatiated. The result suggested that giving jaloh extract to combine it with probiotic, and chromium has no significant effect in hematology profile. When combined extract jaloh with chromium (Cr), has potencial capability to stimulate improvement built of antibody (as immunomodulator) on broiler chicken heat stress.

Seroprevalensi Avian influenza H5N1 pada Unggas di Kabupaten Aceh Utara Darmawi Darmawi; Darniati Darniati; Maryulia Dewi; Fakhrurrazi Fakhrurrazi; Mahdi Abrar; Erina Erina
Jurnal Agripet Vol 13, No 2 (2013): Volume 13, No. 2, Oktober 2013
Publisher : Agricultural Faculty

Seroprevalence of avian influenza H5N1 in birds in north aceh districtABSTRACT. Avian influenza virus H5N1 infections are an important cause of diseases in humans and several animal species, including birds. The present study conducted to investigate the seroprevalence Avian Influenza H5N1 in native birds from 15 sub-districts of North Aceh. This study utilized 1108 serum samples collected from the axilaris vein (left or right) of birds. The standard Hemaglutination Inhibition (HI) assay was conducted at Microbiology Laboratory Faculty of Veterinary Medicine of Syiah Kuala University to determined serum antibody possitive or negative reaction against Avian influenza H5N1. The result showed that seroprevalence Avian influenza H5N1 virus was 4,7 % in North Aceh District. There were nine sub-districts were tested positively by HI test. However, the serum collected from six sub-districts did not react (negative) against Avian influenza H5N1. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus in native birds occured in part of North Aceh District.

Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh (Antibiotic Activities To Staphylococcus aureus Biofilms Of Aceh Cattle Preputium Isolate) Maryulia Dewi; Darmawi Darmawi; Nurliana Nurliana; T. Fadrial Karmil; T Zahrial Helmi; Fakhrurrazi Fakhrurrazi; Erina Erina; Mahdi Abrar; M. Daud AK; Masda Admi
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.35219

AbstractBiofilm is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB) medium were used to biofilm. Bacterial while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were λ 595 nm. Data were analyzed by using Analysis Of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/μL. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/μL. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive. Keywords: biofilm, antibiotics, Staphylococcus aureus, cows aceh. Abstrak Biofilm merupakan salah satu parameter untuk menentukan jalur patogenisitas suatu bakteri. Antibiotik dapat melemahkan aktivitas virulensi bakteri dengan menghambat pembentukan biofilm. Penelitian ini bertujuan untuk melihat karaksteristik dan aktivitas antibiotik terhadap biofilm Stapylococcus aureus isolat preputium sapi Aceh. Penelitian bersifat eksplorasi dan eksperimental, menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan menggunakan tiga jenis antibiotik, tiga konsentrasi dan tiga kali ulangan. Antibiotik yang digunakan adalah tetrasiklin, oksitetrasiklin dan fosfomisin dengan konsentrasi masing-masing 10%, 20% dan 30% (mg/mL). Pengujian biofilm dilakukan pada media Nutrient Broth (NB), menggunakan mikroplat U-bottom polystyrene 96 wells. Pewarnaan biofilm menggunakan kristal violet 1%. Optical Density (OD) biofilm dibaca pada λ 595 nm. Data hasil penelitian dianalisis dengan menggunakan Analysis Of Variance (ANOVA). Hasil penelitian menunjukkan aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm tergolong positif moderat, yaitu 2,667cell/µL. Uji Biofilm menunjukkan jenis dan konsentrasi antibiotik berpengaruh secara nyata (P<0,05) terhadap daya hambat biofilm Staphylococcus aureus isolat preputium sapi Aceh. Tetrasiklin 30% (mg/mL) mempunyai kemampuan paling baik menghambat pembentukan biofilm Staphylococcus aureus isolat preputium sapi Aceh sebesar 52,18% menjadi 1,262 cell/µL, sehingga aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm dari positif moderat menjadi positif palsu. Kata Kunci: biofilm, antibiotik, Staphylococcus aureus, sapi aceh.

Isolasi Dan Identifikasi Bakteri Salmonella Sp. Pada Daging Ikan Kuniran (Upeneus Sulphureus) Di Pasar Lampulo Banda Aceh Nucha Nabila Nur; Fakhrurrazi Fakhrurrazi; Cut Dahlia Iskandar; Faisal Jamin; Roslizawaty Roslizawaty; Ginta Riady
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 4 (2022): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i4.21510

ABSTRAKIkan kuniran merupakan salah satu bahan makanan yang memiliki sumber protein tinggi, mengandung asam amino esensial yang diperlukan oleh tubuh. Kondisi pasar lampulo yang ramai dan kurangnya higienitas dari tempat penjualan ikan, merupakan penyebab ikan terkontaminasi oleh bakteri Salmonella. Penelitian ini bertujuan untuk mendeteksi adanya cemaran bakteri Salmonella sp. pada daging ikan kuniran (Upeneus sulphureus) di Pasar Lampulo Banda Aceh. Penelitian ini menggunakan metode Carter. Sampel yang digunakan dalam penelitian berjumlah 8 sampel yang diambil menggunakan metode observasional. Ikan kuniran diambil sebanyak 1 gram/sampel, lalu digerus dan ditanam pada media SCB (Salmonella Cystine Broth) dan diinkubasi dengan suhu 37oC selama 24 jam. Bakteri yang tumbuh ditanam pada media SSA (Salmonella Shigella Agar) dan diinkubasi dengan suhu 37oC selama 24 jam. Setelah itu dilakukan pewarnaan Gram dan penanaman bakteri pada media NA miring. Media NA miring diinkubasi pada suhu 37ºC selama 24 jam lalu dilakukan uji biokimia. Hasil penelitian dianalisis secara deskriptif dan data disajikan dalam bentuk gambar dan tabel. Dapat disimpulkan bahwa ikan kuniran positif tercemar oleh bakteri Salmonella sp. berdasarkan pemeriksaan mikrobiologi.ABSTRACTKuniran fish is one of the foodstuffs that has a high protein source, containing essential amino acids needed by the body. The crowded conditions of the Lampulo market and the lack of hygiene from fish selling places are the causes of fish being contaminated by Salmonella bacteria. This study aims to detect the presence of Salmonella sp. on the flesh of kuniran fish (Upeneus sulphureus) at the Lampulo Market, Banda Aceh. This study uses the Carter method. The sample used in the study amounted to 8 samples taken using the observational method. Kuniran fish were taken as much as 1 gram/sample, then planted on SCB (Salmonella Cystine Broth) media and incubated at 37oC for 24 hours. The bacteria that grew were grown on SSA (Salmonella Shigella Agar) media and incubated at 37oC for 24 hours. After that, Gram staining and bacteria were planted on slanted NA media. The inclined NA medium was incubated at 37ºC for 24 hours and then a biochemical test was performed. The results of the study were analyzed descriptively and the data was presented in the form of pictures and tables. It can be concluded that the positive kuniran fish contaminated by Salmonella sp. based on microbiological examination.

Invasif dan Persisten Pasteurella multocida Setelah Diinokulasi Secara Intratracheal pada Kalkun Mahdi Abrar; Fakhrurrazi Fakhrurrazi
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

The objective of this study was to evaluate the invasiveness and persistence of Pasteurella multocida after intratracheal inoculation on turkeys. Four strains of Pasteurella multocida, P-1059, T-325, CU, and M-9 were used in this study and intratracheally (IT) inoculated into turkeys. The number of viable bacteria in the trachea, lung, liver, spleen, and the blood were enumerated during periods of 6 and 9 hrs post inoculation (PI). The result showed that four strains of organism were present in the organ and the tissues observed. In the liver, spleen, and blood, the virulent P-1059 strain showed significant increases within 6 or 9 hrs PI. Strain CU showed significant increase only in the spleen. Two other strains did not show significant increase in any organs; strain M-9, on the other hand, showed significant decrease in the trachea. These results indicated that P-1059 and three other strains showed their ability to invade the tissues or organs when they are intratracheally inoculated.

Isolation and Characterization of Avian Influenza Virus Based on Macroscopic Examination of Native Chicken Embryo Fakhrurrazi Fakhrurrazi; Hamdani Budiman
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): September
Publisher : Universitas Syiah Kuala

The aim of this research was to isolate and characterize the virus based on macroscopic examination of native chickens embryo infected with the AI virus. This research used 10 infected native chickens from Ateuk Pahlawan and Doi villages. All chickens were examined to identify the present of AI using rapid test and in ovo virus propagation. Rapid test showed 7 chickens from Ateuk Pahlawan villageand 5 chickens from Doi village were AI positive. Propagation of the viruses in 11 days old chicken embryo in ovo showed that embryo died 5 days post inoculated whereas macroscopic examination showed dwarfed embryo, hemorrhagic, and loss of feather.

Isolasi Dan Identifikasi Bakteri Eschericia Coli Pada Produk Ceker Ayam Bakar Di Gampong Ulee Lheue Kota Banda Aceh Rizki Kurniawan Kurniawan; Darniati Darniati; Mahdi Abrar; Fakhrurrazi Fakhrurrazi; M. Jalaluddin; Erina Erina
JURNAL ILMIAH MAHASISWA VETERINER Vol 7, No 2 (2023): FEBRUARI-APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v7i2.20644

ABSTRAKPenelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Eschericia coli yang mungkin mengontaminasi produk ceker ayam bakar di Gampong Ulee Lheue, Kecamatan Meuraxa, Kota Banda Aceh. Penelitian ini dilakukan berdasarkan metode Carter (1987) yang telah dimodifikasi. Sampel penelitian yang digunakan berjumlah 10 sampel dan diambil dari 5 pedagang yang berbeda. Sampel diisolasi pada media Nutrient Broth (NB) dan diinkubasikan selama 24 jam dengan suhu 37° C. Hasil positif pertumbuhan pada media NB kemudian diinokulasikan pada media Eosin Methylen Blue Agar (EMBA) untuk dilakukan pengamatan morfologi koloni bakteri secara makroskopis. Selanjutnya, dilakukan pewarnaan Gram terhadap koloni bakteri untuk pengamatan morfologi secara mikroskopis. Identifikasi bakteri Eschericia coli dilakukan melalui uji biokimia pada media Indol, Methyl Red-Voges Proskauer (MR-VP), Simmon’s Citrate Agar (SCA), Triple Sugar Iron Agar (TSIA), Sulfide Indol Motility (SIM), serta media gula-gula (glukosa, sukrosa, laktosa, maltosa, dan manitol). Data hasil penelitian ini dianalisis secara deskriptif dan ditampilkan dalam bentuk gambar dan tabel. Hasil penelitian menunjukkan 9 sampel (90%) positif terkontaminasi Eschericia coli yang disimpulkan bahwa produk ceker ayam bakar yang dijual di Gampong Ulee Lheue Kota Banda Aceh telah terkontaminasi oleh bakteri Eschericia coli.Kata kunci: Ceker ayam, Eschericia coli, Foodborne disease, Ulee LheueABSTRACTThis study aims to isolate and identify the possibility of Eschericia coli contamination on grilled chicken feet products in Gampong Ulee Lheue, Meuraxa District, Banda Aceh City. This research is based on the modified of Carter’s (1987) method. The research sampel used were 10 samples and were taken from 5 different traders. Samples were isolated on Nutrient Broth (NB) media and incubated for 24 hours at 37° C. The positive results of growth on NB media were then inoculated on Eosin Methylen Blue Agar (EMBA) media for macroscopic bacterial colonies morphological observations. Furthermore, Gram staining was performed on bacterial colonies for microscopic morphological observations. Identification of Eschericia coli conducted through biochemical tests on Indole, Methyl Red-Voges Proskauer (MR-VP), Simmon's Citrate Agar (SCA), Triple Sugar Iron Agar (TSIA), Sulfide Indol Motility (SIM), and sugars (glucose, sucrose, lactose, maltose, and mannitol). The data from this research were analyzed descriptively and displayed in the form of pictures and tables. The results showed that 9 samples (90%) were positively contaminated with Eschericia coli which concluded that grilled chicken feet products sold in Gampong Ulee Lheue, Banda Aceh City have been contaminated by Eschericia coli bacteria.Keyword: Chicken feet, Eschericia coli, Foodborne disease, Ulee Lheue

Invasif dan Persisten Pasteurella multocida Setelah Diinokulasi Secara Intratracheal pada Kalkun Mahdi Abrar; Fakhrurrazi Fakhrurrazi
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9815

The objective of this study was to evaluate the invasiveness and persistence of Pasteurella multocida after intratracheal inoculation on turkeys. Four strains of Pasteurella multocida, P-1059, T-325, CU, and M-9 were used in this study and intratracheally (IT) inoculated into turkeys. The number of viable bacteria in the trachea, lung, liver, spleen, and the blood were enumerated during periods of 6 and 9 hrs post inoculation (PI). The result showed that four strains of organism were present in the organ and the tissues observed. In the liver, spleen, and blood, the virulent P-1059 strain showed significant increases within 6 or 9 hrs PI. Strain CU showed significant increase only in the spleen. Two other strains did not show significant increase in any organs; strain M-9, on the other hand, showed significant decrease in the trachea. These results indicated that P-1059 and three other strains showed their ability to invade the tissues or organs when they are intratracheally inoculated.

Isolation and Characterization of Avian Influenza Virus Based on Macroscopic Examination of Native Chicken Embryo Fakhrurrazi Fakhrurrazi; Hamdani Budiman
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i2.9788

The aim of this research was to isolate and characterize the virus based on macroscopic examination of native chickens embryo infected with the AI virus. This research used 10 infected native chickens from Ateuk Pahlawan and Doi villages. All chickens were examined to identify the present of AI using rapid test and in ovo virus propagation. Rapid test showed 7 chickens from Ateuk Pahlawan villageand 5 chickens from Doi village were AI positive. Propagation of the viruses in 11 days old chicken embryo in ovo showed that embryo died 5 days post inoculated whereas macroscopic examination showed dwarfed embryo, hemorrhagic, and loss of feather.

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