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The Effectiveness of Lugol on the Increasing of Pregnancy Rate in Aceh Cow with Endometritis Amalia Sutriana; Arman Sayuti; Budianto Panjaitan; Teuku Armansyah TR; Aisyah Fadillah Tunnisa; Juli Melia; Tongku Nizwan Siregar; Hafizuddin Hafizuddin; Dwinna Aliza
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.18513

Abstract

ABSTRACT. The objective of this study was to determine the effect of lugol on the increasing the pregnancy rate in repeat breeding (RB) Aceh cows due to endometritis. This study used six endometritis’s cows, aged 5-7 years, weighed 150-250 kg which were divided into two groups (n=3), KI and KII. The cows in group 1 (K1) were injected with 5 ml PGF2α, while the cows in group 2 (KII) were treated with 50 ml of 2% lugol intra-uterine and continued with an injection of 5 ml PGF2α after healing. The detection of estrus was performed twice a day following by artificial insemination (AI) about 10-16 hours after the onset of estrus. Determination of pregnancy was performed by ultrasonography (USG) on the 25th day after AI. The data obtained were analyzed descriptively. The results showed that all endometritis ’cows in KI and KII present estrous signs (100%). However, only one cow was recovered in K2, whereas in K1 did not. After AI, one pregnant cow was observed in KII (33.3%), while none of the pregnant cows was found in K1 (0.0%). It is concluded that the lugol treatment for endometritis’s Aceh cows can improve the pregnancy rate. (Efektivitas larutan lugol untuk meningkatkan persentase kebuntingan pada sapi Aceh yang mengalami endometritis) ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh pemberian larutan lugol terhadap peningkatan persentase kebuntingan sapi Aceh yang mengalami RB. Dalam penelitian ini digunakan enam ekor sapi Aceh betina dewasa, umur 5-7 tahun, bobot badan 150-250 kg, sudah pernah beranak, dan didiagnosis mengalami endometritis. Seluruh sapi dibagi menjadi dua kelompok (n=3). Pada kelompok 1 (K1), sapi endometritis diterapi dengan 50 ml lugol 2% secara intra uteri dan setelah sembuh dilanjutkan dengan penyuntikan 5 ml PGF2α. Sapi pada kelompok 2 (K2) hanya diinjeksi dengan 5 ml PGF2α. Deteksi berahi dilakukan sebanyak dua kali per hari dan inseminasi buatan (IB) dilakukan sekitar 10-16 jam setelah awal berahi. Pemeriksaan kebuntingan dilakukan dengan ultrasonografi (USG) pada hari ke-25 setelah IB. Data dianalisis secara deskriptif. Dari masing-masing kelompok, hanya satu ekor sapi pada K2 yang dinyatakan sembuh yaitu sapi pada kelompok kedua. Persentase sapi yang menjadi estrus pada kedua kelompok masing-masing adalah 100%. Dari tiga ekor sapi yang diinseminasi pada masing-masing kelompok, hanya satu ekor sapi pada K2 (33,3%) yang menunjukkan hasil positif bunting sedangkan pada K1 tidak terdapat sapi yang menunjukkan hasil positif (0,0%). Disimpulkan bahwa pemberian larutan lugol pada sapi Aceh yang mengalami endometritis dapat meningkatkan persentase kebuntingan sapi Aceh.
Histologi, Histomorfometri, dan Histokimia Usus Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas Bagus Dwijayanti; Erdiansyah Rahmi; Ummu Balqis; Fitriani Fitriani; Dian Masyitha; Dwinna Aliza; Muslim Akmal
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.16012

Abstract

ABSTRACT. Usus adalah salah satu organ pencernaan yang berperan penting pada proses absorpsi nutrisi sebagai sumber energi. Penelitian ini bertujuan mengetahui histologi, histomorfometri dan histokimia sebaran glikogen pada usus ayam buras (Gallus gallus domesticus) sebelum dan setelah menetas menggunakan pewarnaan Haematoksilin-Eosin (HE) dan pewarnaan Periodic Acid Schiff (PAS). Sampel penelitian terdiri atas empat kelompok tingkat umur berbeda, setiap kelompok berjumlah enam usus ayam. Hasil penelitian menunjukkan perbedaan nyata (P˂0,05) morfometri usus ayam buras secara makroskopis. Secara histologis pada setiap perkembangan sudah terlihat lapisan usus yang terdiri atas tunika mukosa, submukosa, muskularis, dan serosa. Pada tunika mukosa, didapati tiga lamina yaitu lamina epitelia, propria, dan muskularis mukosa yang mulai teramati pada masa inkubasi hari ke-14. Lamina epitelia berupa sel epitel silindris selapis dengan mikrovili, lamina propria berupa jaringan ikat, dan lamina muskularis mukosa berupa berkas otot polos. Pada tunika mukosa dijumpai adanya sel Goblet dan kripta Liberkuhn yang teramati pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7. Hasil histomorfometri ketebalan lapisan usus, panjang vili, lebar vili, dan diameter lumen usus ayam buras pada masa inkubasi dan setelah menetas menunjukkan perbedaan nyata (P˂0,05). Temuan reaksi positif kuat terhadap pewarnaan PAS dijumpai pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7 dan positif lemah pada masa inkubasi hari ke-14. Dapat disimpulkan bahwa terdapat korelasi antara perkembangan usus ayam secara makroskopis, histomorfometri ketebalan tunika dan vili, serta sebaran reaksi positif kuat terhadap pewarnaan PAS pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7.  (Histological, histomorphometrical, and histochemical of intestine on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRAK. The intestines are one of the digestive organ that play an important role in the nutrient absorption process as an energy source. The aim of this study was to determine the histomorphometric and histochemical of glycogen distribution in the intestines of native chickens pre and post hatching periods using Haematoxylin-Eosin (HE) staining and Periodic Acid Schiff (PAS) staining. The samples of this study was consisted of four different age groups, each group consisting of six chicken intestines. The results showed a significant difference (P˂0.05) on macroscopic morphometry of the native chicken’s intestine. Histologically in each group has been found the intestines consisting of mucosa, submucous, muscularis, and serous tunic. In the mucosal tunic, there were three layers; ephitelial, propria, and muscularial mucous can be observed during the 14th day of incubation period. Ephitelial layer in the form of cylindrical epithelial cells with microvilli, proprial layer in the form of connective tissue, and layer of the muscularis mucosa form smooth muscle thread. In the mucosal tunic, there was Goblet cells and Liberkuhn crypts that can be observed during the 20th day incubation period and 7th day post hatch. Histomorphometry results of intestinal lining thickness, villi length, villi width, and lumen diameter of native chicken intestine during incubation and post hatching showed significant differences (P˂0.05). The findings of a strong positive reaction to the staining of PAS were found on the incubation period of the 20th day and post hatching the 7th day and positively weak on the incubation period of the 14th day. It can be concluded that there is a correlation between macroscopic development of chicken intestine, histomorphometric of tunica and villous thickness, as well as the distribution of strong positive reactions to the staining of PAS during the incubation period of the 20th day and post hatching the 7th day.
26. GAMBARAN HISTOPATOLOGIS GINJAL TIKUS PUTIH (Rattus novergicus) JANTAN YANG DIINFEKSIKAN Trypanosoma evansi DAN DIBERI EKSTRAK DAUN SERNAI (Wedelia biflora) (Histopathology of Male Rat (Rattus novergicus) Kidney Infected with Trypanosoma evansi and Treated with Sernai Leaves Extract (Wedelia biflora)) Yudha Fahrimal; Rahmiwati R; Dwinna Aliza
Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.449 KB) | DOI: 10.21157/j.med.vet..v10i2.4386

Abstract

This research was aimed to study histopathology of rat kidney infected with Trypanosoma evansi after administration of sernai leaves extract (Wedelia bifolora). The samples used were kidneys of 25 male rats strain Wistar which were equally divided into 5 treatment groups. Group P1 was uninfected and untreated control, group P2 was infected with 1x103 T. evansi but not sernai leaves extract, rats from groups P3, P4, and P5 were infected with 1x103 T. evansi and given sernai leaves extract with the dose of 30 mg/kg bw, 45 mg/kg bw, and 60 mg/kg bw respectively. The sernai extract was administered orally using a stomach tube for 3 consecutive days. The next day after last treatment, all rats were sacrificed and necropsied. Kidneys were collected for histopathological examination such as degeneration and necrosis of tubules cells, convoluted contortus duct lumen, and adhesion and atrophy of glomerulus. Sernai leaves extract with the dose of 45 mg/kg bw was the most effective dose to prevent kidney damage due to T. evansi infection.
9. The Effectiviteness of Jatropha salve (Jatropha curcas L) in Inflammatory Phase of cutaneous wound healing in Mice (Mus musculus) Histopathologically Indra Sitorus; M Nur Salim; Nazaruddin Nazaruddin; Dwinna Aliza; Denny Irmawati; Awaluddin Awaluddin; Cut Nila Thasmi; Muslim Akmal; Dian Masyitha
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.4374

Abstract

This study aims to determine the effectiveness of 10% Jatropha salve (Jatropha curcas L. )    to inflammation reaction cutaneous wound healing in mice (Mus musculus ) in the inflammatory phase. This experiment used 9 male mices, weighing 25-40 grams and age 2-3 months old, were divided into three treatment groups, each treatment consisting of three mices. The wound were made along 2 cms in the back area. Wound therapy was carried out  twice a day for 3 days.  Group KI was administered  and given by yellow vaseline, KII was given 10% Jatropha salve, and KIII was given gentamicin salve. The parameters measured are the increase in the number infiltrations of inflammatory cells,  neovascular, and re-epitelization. All quantitative data were measured using ANOVA and continued with Duncan Test, the qualitative data were presented descriptively by showing the results of observation under a microscope. The statistical test on the infiltrations of inflammatory cells and neovasculars parameter 10% Jatropha salve group significantly different (P0.05) compared to KI and KIII group. Histopatologycal observations indicated the 10% Jatropha salve could be reduce the infiltrations of inflammatory cells, increase neovasculars and re-epitelization percentage. According to the result can be concluded the 10% Jatropha salve can accelerate the inflammation phase cutaneous wound healing in mice.
35. The distribution of melanomacrophage center in liver and kidney of Tilapia fish that infected by Aeromonas hydrophilic Dian Pratiwi; Dwinna Aliza; Nazarudin Nazarudin; Zuhrawati Zuhrawati; Hamdani Budiman; Razali Daud
Jurnal Medika Veterinaria Vol 13, No 2 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i2.3648

Abstract

This research aims was to determine the number of melanomacrophage center (MMC) in the liver and kidney of tilapia (Oreochromis niloticus) were injected A.hydrophila. This research uses 15 tilapia fish with an average weight of 200-300 g were divided into 5 groups, the control group, and four treatment groups. All treatment groups were injected bacteria A. hydrophila 10-5 CFU dose as much as 0.01 ml intramuscularly. Kidney and liver of tilapia in the control group and K1 were collected on day 1 post-injection, K2 on day 2 post-injection, K3 on day 3 post-injection, K4 on day 4 post-injection, and K5 on day 5 post-injection. MMC Joomla Data were analyzed by ANOVA. Results of the study group K0, K1, K2, K3, K4, and K5 in the liver was 20 ± 18.19 K0, K1 55.67 ± 38.81, 69.67 ± 18.19 K2, K3 80.33 ± 6, 42, 84 ± 6 K4, K5 88.33 ± 4.9. While the results of the kidneys was 32 ± 4.0 K0, K1 57 ± 41.9, 112.3 ± 16.4 K2, K3 113.3 ± 15.3, 96.3 ± 13.5 K4, K5 119.6 ± 30.4. Conclusion The infection of the longer period the higher the number of MMC.
PENGARUH UMUR TERHADAP PENJANTANAN LARVA IKAN LELE DUMBO (Clarias gariepinus) YANG DIRENDAM MENGGUNAKAN HORMON METIL TESTOTERON ALAMI (The Influence of Age on Masculinization of Clarias gariepinus Larvae Immersed by Methyl Testosterone Natural Hormone) Hattanul Mulia; Rosmaidar Rosmaidar; Dasrul Dasrul; Dwinna Aliza; Dian Masyitha; Sugito Sugito
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.017 KB) | DOI: 10.21157/j.med.vet..v10i1.4036

Abstract

This research aims to examines the influence of age on masculinization of Clarias gariepinus larvae immersed by methyl testosterone (MT) natural hormone. This research used 270 catfish larvae divided into 3 groups of age those are 5, 10, and 20 days. The research implemented complete random design (CRD). Immersion was performed using methyl testosterone hormone at the dose of 0.25 g/L for 20 hours. Observation of fish was done after 60 day-old fish. Data obtained was analyzed using one-way analysis of variance The average percentages of male-sex catfish after immersing in natural MT on group I, II, and III were 97.67, 91.07, and 86.67%, respectively. The results showed that the age of the larvae did not affect significantly (P0.05) on the percentage of male sex of Clarias gariepinus. The average percentage of male sex in 5 days age group was higher than 10 and 20 days age group. In conclusion, the masculinization of Clarias gariepinus larva immersed by MT natural hormone is not influenced by age of fish before dipping.
The Effect of Dipping Time in Natural Methyl Testosterone Hormone on Male Forming Succesiveness Process of Tilapia (Oreochromis niloticus) Larvae Rosmaidar Rosmaidar; dwinna aliza; Jesica Ramadhanita
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.041 KB) | DOI: 10.21157/j.med.vet..v8i2.3361

Abstract

The purpose of this research was to know the influence of dipping time in natural methyl testosterone hormone (MT) on successful male forming process of tilapia (Oreochromis niloticus) larvae. This research used 360 7 days old tilapia larvae, purchased from cultivation of brackish water, Ujung Batee, Banda Aceh. The samples were divided into 4 treatment groups with three repetitions, they were P0 (control group without treatment), PII (Tilapia larvae were dipped in natural MT for 10 hours), PII (Tilapia larvae were dipped in natural MT for 20 hours), and PIII (Tilapia larvae were dipped in natural MT for 30 hours). All tilapia larvae were maintained until the age of 60 days, before the sex of the fish examined. The average percentage of male tilapia in group P0, PI, PII, and PIII were 50.28, 87.77, 90.06, and 95.05%, respectively. Statistical analysis showed that there were significant differences (P0.05) among P0, PI, PII, and PIII. Based on result of this research, it can be concluded that the longest dipping time the highest the percentage of male tilapia.Key words: larvae, tilapia, natural hormone methyl testosterone, the male sex percentage
Behavior and Gill profile of Nile Tilapia (Oreochromis niloticus) Induced by Density stress Dwinna Aliza
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.195 KB) | DOI: 10.21157/j.med.vet..v8i1.3344

Abstract

 The aim of this research was to observe behavior and gill profile of nile tilaphia (Oreochromis niloticus) induced by density stress. A total of 50 nile tilapia fish with the age of 3-8 months and 80-100 bwwere divided into 4 groups. Group I was control group contained 5 fish, group II,III, and IV contained 10, 15, and 20 fish, respectively. Treatment was performed for 24 hours, and fish behavior was observed every 3 hours. Gillsample was examined by anatomical pathology then tissue samples were collected and fixed in 10% Davidson solution, routinely processed,embedded in paraffin, sectioned at 5 microns, stained with hematoxylin and eosin (HE), and examined by light microscopy. The data wasanalyzed descriptively. Behavior changessuch as swim up to water surface and rapid movement of operculum occur earlier than passivemovement and decreased reflex of fish on group II, III, and IV. The anatomical pathology of nile tilapia gills observed were darker colour ofgills, gill rott, and over mucus.Histopathological result showed that haematoma, necrosis, hyperplasia of primary and secondary lamella, andfusion lamella were observed in gills of nile tilapia induced by density stress.____________________________________________________________________________________________________________________Key words: histopathology, nile tilapia, gill, density stress 
Pemberian Ekstrak Akar Pasak Bumi Meningkatkan Kualitas Spermatozoa Domba Waringin Amalia Sutriana; Mira Ayu Lestari Hasibuan; Nurhazimah Nurhazimah; Teuku Armansyah; Tongku Nizwan Siregar; Budianto Panjaitan; Hafizuddin Hafizuddin; Arman Sayuti; Dwinna Aliza; Rosmaidar Rosmaidar
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.503 KB) | DOI: 10.19087/jveteriner.2021.3.317

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak akar pasak bumi (Eurycoma longifolia Jack) terhadap peningkatan konsentrasi testosteron domba Waringin. Dalam penelitian ini digunakan dua ekor domba waringin jantan berumur ±4 tahun. Penelitian ini merupakan penelitian eksperimen dengan menggunakan rancangan pola bujur sangkar latn 2 x 2 sehingga hewan percobaan diberi perlakuan 20 mL akuades per oral sebagai kontrol (P1) dan 20 mL larutan yang mengandung 45 mg/kg bobot badan ekstrak pasak bumi yang diberikan per oral (P2). Perlakuan diberikan selama enam hari pada pukul 09.00 WIB. Pada bulan ke-1 domba pertama mendapat perlakuan P1 sedangkan domba kedua mendapat perlakuan P2. Sebaliknya, pada bulan ke-2 domba pertama mendapat perlakuan P2 sedangkan domba kedua mendapat perlakuan P1. Koleksi darah untuk pemeriksaan konsentrasi hormon testosteron dilakukan lima jam setelah pemberian ekstrak akar pasak bumi (Eurycoma longifolia Jack). Analisis konsentrasi testosteron dilakukan menggunakan metode enzyme linked immunosorbent assay (ELISA) yang diukur pada hari 1, 3 dan 6. Data yang diperoleh dianalisis dengan uji sidik ragam pola bujur sangkar latin. Hasil analisis statistika menunjukkan bahwa volume semen, motilitas spermatozoa, viabilitas spermatozoa dan abnormalitas spermatozoa pada domba yang diberikan ekstrak akar pasak bumi (E. longifolia Jack) menunjukkan perbedaan yang signifikan (P<0,05) dibandingkan dengan kontrol namun konsentrasi testosteron tidak menunjukkan perbedaan yang signifikan (P>0,05). Disimpulkan bahwa pemberian ekstrak akar pasak bumi meningkatkan kualitas spermatozoa namun tidak menyebabkan peningkatan konsentrasi hormon testosteron pada domba waringin.
ANGKA PREVALENSI CEMARAN BAKTERI Escherichia coli PADA MEJA DAN PERALATAN PEDAGANG DAGING AYAM BROILER DI DUA PASAR TRADISIONAL KOTA BANDA ACEH (The Prevalence Rate of Escherichia coli Contamination on the Table and Equipment of Broiler Traders at Two Traditional Markets in Banda Aceh) chiara giri puspa; T Reza Ferasyi; Rastina Rastina; dwinna aliza; fadli a gani; mahdi abrar
JURNAL ILMIAH MAHASISWA VETERINER Vol 4, No 3 (2020): MEI-JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v4i3.15179

Abstract

ABSTRAKEscherichia coli dikenal sebagai bakteri indikator sanitasi, keberadaanya dalam pangan menunjukkan bahwa pangan tercemar oleh lingkungan. Penelitian ini bertujuan mengetahui angka prevalensi bakteri E. coli pada meja dan peralatan pedagang daging ayam broiler. Sampel yang digunakan yaitu 20 swab meja, pisau, dan talenan pedagang daging ayam broiler di dua Pasar Tradisional Kota Banda Aceh. Penelitian ini menggunakan metode Total Plate Count (TPC) dengan larutan Buffer Peptone Water (BPW) dan mengidentifikasi E. coli menggunakan media selektif diferensial, yaitu dengan Eosin Methylene Blue Agar (EMBA). Hasil penelitian menunjukkan bahwa 2 meja, 2 pisau, dan 3 talenan di Pasar Tradisional Seutui positif tercemar E. coli dengan angka prevalensi cemaran E. coli pada meja (40%), pisau (40%), dan talenan (60%), sedangkan di Pasar Tradisional Peunayong terdapat 2 meja dan 2 pisau positif tercemar E. coli dengan angka prevalensi cemaran  E. coli pada meja (13%) dan pisau (13%). Hasil kesimpulan yang didapat yaitu rata-rata angka prevalensi cemaran E. coli secara keseluruhan pada peralatan pedagang dari dua Pasar Tradisional di Kota Banda Aceh yaitu 18,33%.Kata kunci : Escherichia coli, prevalensi, peralatan pedagang, pasar tradisional  ABSTRACTEscherichia coli is known as an indicator of sanitation bacteria, its presence in food indicates that food is polluted by the environment. This study aims to determine the prevalence of  E. coli bacteria on the table and equipment of broiler chicken meat traders. The samples used were 20 table swabs, knives, and cutting boards of broiler traders in two Traditional Markets in Banda Aceh City. This study uses the Total Plate Count (TPC) method with Buffered Peptone Water (BPW) solution and identifies E. coli using differential selective media, namely Eosin Methylene Blue Agar (EMBA). The results showed that 2 tables, 2 knives, and 3 cutting boards in the Seutui Traditional Market were positively contaminated with E. coli with the prevalence of E. coli contamination on the table (40%), knives (40%), and cutting boards (60%), while in the Peunayong Tradisional Market there are 2 tables and 2 positive knives contaminated with E. coli with the prevalence rate of E. coli contamination on the table (13%) and knives (13%). The conclusion obtained is the average prevalence rate of E. coli contamination as a whole on the equipment of traders from two Traditional Markets in the City of Banda Aceh is 18.33%. Key words : Escherichia coli, prevalence, equipment traders, traditional markets