Siti Rani Ayuti
Fakultas Kedokteran Hewan, Universitas Syiah Kuala

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Potensi Khamir Kluyveromyces lactis dalam Meningkatkan Kualitas Susu Fermentasi yang Ditambah Jagung Manis (Zea mays saccharata Linn) Siti Rani Ayuti; Yurliasni Yurliasni; Cut Intan Novita; Elmy Mariana
Jurnal Agripet Vol 20, No 1 (2020): Volume 20, No. 1, April 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.329 KB) | DOI: 10.17969/agripet.v20i1.15356

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui peningkatan kualitas produk susu fermentasi dengan menggunakan khamir Kluyveromyces lactis dan penambahan jagung manis. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri dari 2 faktor dan 2 level, yaitu faktor penambahan jagung manis (Zea mays saccharata Linn) (A) terdiri atas 25% (a1) dan 75% (a2), dan faktor penambahan khamir Kluyveromyces lactis (B) terdiri atas 0.5% (b1) dan 1% (b2), dengan lima kali ulangan pada masing-masing perlakuan sehingga memperoleh 20 satuan percobaan. Parameter yang diamati antara lain adalah kadar protein, kadar lemak, kadar asam laktat dan derajat keasaman (pH). Data yang diperoleh dianalisis secara statistik menggunakan ANOVA dan dilanjutkan dengan uji lanjut Duncan. Hasil penelitian menunjukkan bahwa penambahan khamir Kluyveromyces lactis tidak berpengaruh nyata (P0.05) terhadap kadar protein, lemak, kadar asam laktat dan derajat keasaman (pH). Sementara penambahan jagung manis tidak berpengaruh nyata (P0.05) terhadap kadar protein dan derajat keasaman (pH), namun berpengaruh sangat nyata (P0.01) terhadap kadar lemak dan kadar asam laktat. Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan khamir Kluyveromyces lactis 1% dan jagung manis (Zea mays saccharata Linn) 75% pada produk susu fermentasi dapat meningkatkan kualitas produk susu.  (Potential of yeast-Kluyveromyces lactis in improving quality of fermented milk added sweet corn (Zea mays saccharata Linn)) ABSTRACT. The aims of this research were to determine the quality improvement of fermented milk products using Kluyveromyces lactis and the addition of sweet corn. This study used factorial experiment conducted with completely randomized design (CRD) which divide into 2 factors and 2 levels, there were the addition of sweet corn (Zea mays saccharata Linn) factors (A) consist of 25% (a1) and 75% (a2), and the addition of yeast Kluyveromyces lactis factors (B) consist of 0.5% (b1) and 1% (b2), with five replications on each treatment to obtain 20 units of experiment. The observed parameters such as protein levels, fat levels, lactic acid levels and acidity degree (pH). Research data were analyzed statistically by using ANOVA and continued by Duncan's tests. The results showed that the addition of yeast Kluyveromyces lactis had no significant effect (P 0.05) on protein levels, fat levels, lactic acid levels and acidity degree (pH). While the addition of sweet corn had no significant effect (P 0.05) on protein levels and acidity degree (pH), but it had a very significant effects (P 0.01) on fat levels and lactic acid levels. The results indicate the addition of yeast Kluyveromyces lactis 1% and sweet corn (Zea mays saccharata Linn) 75% in fermented milk products can improve the quality of dairy products.
Dinamika Pertumbuhan Lactobacillus casei dan Karakteristik Susu Fermentasi Berdasarkan Suhu dan Lama Penyimpanan Siti Rani Ayuti; Nurliana Nurliana; Yurliasni Yurliasni; Sugito Sugito; Darmawi Darmawi
Jurnal Agripet Vol 16, No 1 (2016): Volume 16, N0. 1, April 2016
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.67 KB) | DOI: 10.24815/jn.v%vi%i.3476

Abstract

ABSTRAK. Mutu susu fermentasi sangat dipengaruhi oleh bahan baku, proses pengolahan, proses fermentasi, dan penyimpanan. Perubahan nilai gizi dapat terjadi karena variasi dan fluktuasi suhu dan penyimpanan akan mempercepat kerusakan susu fermentasi. Penelitian bertujuan untuk mempelajari pengaruh suhu dan lama penyimpanan yang berbeda pada susu fermentasi terhadap pertumbuhan L. casei dan karakteristik susu fermentasi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri dari 2 faktor yaitu faktor A (suhu) terdiri dari a1=4-10⁰C, a2=10-16⁰C. Faktor B (lama penyimpanan) terdiri b1=0 hari b2=30 hari, b3=60 hari, b4=90 hari dan dianalisis meliputi jumlah total bakteri L. casei, nilai derajat keasaman (pH), kadar asam laktat, sineresis, dan kadar alkohol. Hasil analisis menunjukkan bahwa perlakuan suhu dan lama penyimpanan berpengaruh sangat nyata (P0.01) terhadap total bakteri, sineresis, kadar alkohol, asam laktat, tetapi pada pH terjadi interaksi antara suhu dan penyimpanan. Disimpulkan bahwa suhu dan lama penyimpanan dapat mengakibatkan pertumbuhan L. casei tidak stabil dan terjadinya perubahan karakteristik fisik susu fermentasi L. casei.  (Growth dynamics of Lactobacillus casei and characteristics of milk fermentation based on temperature and storage time) ABSTRACT. Fermented milk quality is strongly influenced by raw materials, processing, fermentation and storage. Changes in nutritional value may occur due to variations and fluctuations in temperature and storage will accelerate damage fermented milk. The research aimed to study the effect of temperature and different storage length of fermented milk on the growth of L. casei and characteristics of fermented milk. The study used completely randomized design (CRD) with factorial pattern which are consisted of two factors. Factor A (storage temperature) they were a1=4-10⁰C, a2=10-16⁰C. Factor B (storage time) they were b1=0 days, b2=30 days, b3=60 days, b4=90 days and analyzed include the total number of bacteria L. casei, the value of the degree of acidity (pH), lactic acid levels, syneresis, and alcohol content. The results showed that the temperature and storage time was highly significant (P0.01) effect the total bacteria, syneresis, alcohol level, lactic acid level, and there was interaction between temperature and storage time on pH. It can conclude that the temperature and storage time affect the growth of L. casei unstable and changes in the physical characteristics of L. casei fermented milk.
2. Effect of the Addition of Palm Kernel and Ammoniated Lemongrass Waste (Cymbopogon Nardus) on the Quality of Fresh Semen of Thin Tailed Sheep as a Partial Replacement of Basal Feed Fitrah Khairi; Cut Islami Dini; Cut Intan Novita; Siti Rani Ayuti
Jurnal Medika Veterinaria Vol 15, No 2 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i2.24563

Abstract

In this study, three sheep aged two years old with a bodyweight of 20-25 kg were used. The treatments consisted of R1 (10% palm kernel cake and ammoniated citronella waste), R2 (20% oil palm kernel cake and ammoniated citronella waste) and R3 (30% oil palm kernel cake and ammoniated citronella waste). Research design used was Latin Square Design with three treatments and three replications. The parameters of semen observed were macroscopic and microscopic quality which included color, odor, volume, consistency, pH, concentration, mass movement and individual motility. The results showed that the administration of palm kernel cake with concentrations of 10%, 20%, and 30% and ammoniated citronella waste had no significant effect (P0.05) on the quality of fresh thin-tailed sheep's semen both macroscopically and microscopically. The semen had an average volume of 0.62 ml, creamy in color, had a characteristic as semen odor, thick in consistency and has a normal pH of 6.89. Microscopically, it had average concentration of 1809x106/ml, average mass movement of ++, and  average individual motility of 57.57%. Based on the results, the administration of palm kernel cake and ammoniated citronella waste does not affect the quality of the fresh semen of thin-tailed sheep, both macroscopically and microscopically.
Effect of Temperature and Storage Time on The Quality of Quail Egg (Coturnix coturnix japonica) Siti Rani Ayuti; Monita Previa Alasa; Rastina Rastina; Hennivanda Hennivanda; Muhammad Hambal; Abbas Abbas
Jurnal Medika Veterinaria Vol 15, No 2 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i2.26918

Abstract

Many people favor quail eggs because they contain good nutrients, and are easy to obtain at an affordable price. This research was conducted to study the effect of temperature and storage time on the quality of quail eggs. The samples in this study were 66 quail eggs which were divided into two groups, the first group (33 eggs) was stored at 27oC, and the second group (33 eggs) was kept at 5oC. This study used a completely randomized design with two factors, temperature and storage time. The observation was carried out on days 1, 7, 13, 19, 25, and 31 with triplicates. The parameters used in this study were air sac height, egg yolk index, white egg index, and Haugh Unit (HU). This study showed that temperature and storage time affected the quality of quail eggs. Eggs stored at 27oC showed a quality decrease indicated by higher air sac position, and lower egg yolk index and egg white index. Eggs kept at 5oC showed no significant degradation. The HU for both groups ranged between 76-81 which implied that the eggs had AA quality even after 31 days of storage.
PENGARUH PENAMBAHAN PERASAN AIR JERUK PURUT (Citrus hystrix) PADA PROSES PEMBUATAN TELUR ASIN TERHADAP JUMLAH TOTAL BAKTERI Riski Ayu Maulida; Azhari Azhari; Rastina Rastina; Siti Rani Ayuti; Fakhrurrazi Fakhrurrazi; M Daud AK; T Armansyah TR
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 3 (2022): MEI-JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i3.18942

Abstract

ABSTRAK          Telur itik merupakan salah satu produk hasil pengawetan telur dengan cara pengasinan. Penelitian ini bertujuan mengetahui pengaruh penambahan air perasan jeruk purut (Citrus hystrix) pada proses pembuatan telur asin terhadap jumlah total cemaran bakteri. Metode penelitian yang digunakan adalah metode rancangan acak lengkap (RAL) yang terdiri atas 4 perlakuan dan 3 kali ulangan. Perlakuan yang diberikan adalah penambahan larutan perasan jeruk purut dengan konsentrasi 0%, 10%, 20% dan 30% ke dalam media adonan pengasinan telur itik. Pengamatan jumlah total bakteri dilakukan setelah proses pengasinan telur dilakukan yaitu setelah 14 hari masa penyimpanan telur. Pengamatan jumlah total bakteri dilakukan dengan  metode Total Plate Count (TPC) menggunakan media Plate count agar (PCA).  Data jumlah bakteri yang diperoleh di analisis secara statistik menggunakan Analysis Of Variance (ANOVA). Hasil penelitian menunjukkan bahwa penambahan larutan perasan jeruk purut dengan konsentrasi 0%, 10%, 20% dan 30% ke dalam adonan pengasin telur selama proses pembuatan telur asin dapat menurunkan jumlah total bakteri secara signifikan. Hasil uji lanjut menggunakan uji Duncan diketahui bahwa penambahan larutan perasan jeruk purut dengan konsentrasi 30% secara nyata dapat menurunkan jumlah total bakteri pada telur asin yang dihasilkan dibandingkan dengan konsentrasi 0%, 10%, 20%. Sedangkan pembahan larutan perasan jeruk purut dengan konsentrasi 10% sampai dengan 20% tidak dapat menurunkan jumlah total bakteri pada telur asin yang dihasilkan. Berdasarkan penelitian ini dapat disimpulkan bahwa penambahan larutan perasan jeruk purut dengan konsentrasi 30% ke dalam adonan pengasin telur dapat menurunkan jumlah total bakteri pada telur asin yang dihasilkan. ABSTRACT            Duck egg is a product of egg preservation by salting.This study aims to determine the effect of adding citrus hystrix to the process of making salted eggs to the total amount of bacterial contamination. The research method used is a complete randomized design method (RAL) consisting of 4 treatments and 3 replays. The treatment given is the addition of a solution of orange juice purut with a concentration of 0%, 10%, 20% and 30% into the medium of duck egg marinating dough. Observation of the total number of microbes is done after the process of salting eggs is done after 14 days of egg acidification. Observation of the total number of microbes is done by the Method of Total Plate Count (TPC) using media Plate count agar (PCA) Data on the number of microbes obtained in statistical Analysis Of Variance (ANOVA). The results showed that the addition of a solution of orange juice purut with concentrations of 0%, 10%, 20% and 30% into the egg marinating dough during the process of making salted eggs can significantly decrease the total number of microbes. The results of further tests using Duncan's test found that the addition of a solution of orange juice purut with a concentration of 30% can significantly decrease the total number of microbes in the salted eggs produced compared to the concentration of 0%, 10%, 20%. While the deity of orange juice solution purut with a concentration of 10% to 20% can not decrease the total number of microbes in salted eggs produced. Based on this research, it can be concluded that the addition of a solution of orange juice purut with a concentration of 30% into the egg marinating dough can decrease the total number of microbes in the resulting salted eggs.