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Yahdiana Harahap
Departemen Farmasi FMIPA, Universitas Indonesia
Health Professions Medicine & Pharmacology

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UJI SITOTOKSISITAS BUAH MERAH, MAHKOTA DEWA DAN TEMU PUTIH TERHADAP SEL KANKER SERVIKS Radji, Maksum; Aldrat, Hendri; Harahap, Yahdiana; Irawan, Cosphiadi
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 5, No 1 (2010)
Publisher : Indonesian Research Gateway

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Abstract

The cytotoxic effect of herbal medicines has been examined using HeLa cells line (cervical cancer cell culture). The result showed that the LC50 value of buah merah [Pandanus conoideus Lam.], mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.] and temu putih [Curcuma zedoaria (Berg.) Roscoe] extracts were  421 µg/ml, 835µg/ml and 58,9 µg/ml after 24 hour incubation, whereas the LC50 of each extract were 276.79 µg/ml, 415,9 µg/ml and 29.19 µg/ml after 48 hour incubation respectively. The cytotoxic activity of temu putih [Curcuma zedoaria (Berg.)] extract to HeLa cells was stronger than buah merah [Pandanus conoideus Lam.] and mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.] extracts.   ABSTRAK Telah dilakukan penelitian efek sitotoksisitas ekstrak buah merah [Pandanus conoideus Lam.], mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.] and temu putih [Curcuma zedoaria (Berg.) Roscoe] terhadap sel HeLa (kultur sel kanker serviks). Hasil penelitian menunjukkan bahwa nilai LC50 dari ketiga ekstrak tersebut masing-masing adalah 421 µg/ml, 835µg/ml and 58,9 µg/ml, setelah waktu inkubasi selama 24 jam, sedangkan LC50 setelah diinkubasi selama 48 jam adalah  276.79 µg/ml, 415,9 µg/ml and 29.19 µg/ml. Aktivitas sitotoksik  temu putih [Curcuma zedoaria (Berg.)] terhadap sel HeLa  lebih kuat dibandingkan dengan buah merah [Pandanus conoideus Lam.] dan mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.]
UJI SITOTOKSISITAS IN VITRO SEDIAAN JADI BRM (BIOLOGICAL RESPONSE MODIFIER) TERHADAP SEL KANKER SERVIKS Harahap, Yahdiana; Reksodiputro, Harryanto; Heffen, Wan Lelly; Krismartina, Mirna
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 3, No 2 (2006)
Publisher : Indonesian Research Gateway

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Abstract

Cancer was the third big death-caused disease in the developed countries. To overcome the disease, cancer patient can use combinaton of conventional and alternative therapy, such as using herbal   medicine. Nowadays there are many anti cancer herbal medicines in the market, one of which was BRM (Biological Response Modifier), that contain tansinon, polisaccharide, Lycium barbarum, Codonopsis pilosulae Radix extract, and marine algae extract. To investigate the cytotoxic effect of BRM, we  conducted cytotoxicity test in vitro using cancer cell culture. The test examined the activity of ethanol and aquoeus extract of BRM  on Ca Ski cells (cervix cancer cells), using Neutral Red Intake Cytotoxicity Test Method.  Results of the experiment revealed the LC50 of ethanol and aquoeus extract were 274.16 and 55.21mg/ml consecutively, after 24 hour incubation and 118.58 and 35.17 mg/ml after 48 hours incubation. It was concluded that ethanol and aquoeus extract of BRM had low cytotoxicity due to LC50 value greater than 20 mg/ml. ABSTRAK Kanker merupakan penyebab kematian ketiga terbesar di negara berkembang. Untuk mengatasi penyakit tersebut, penderita kanker dapat mengkombinasikan terapi konvensional dengan terapi alternatif, yaitu dengan menggunakan sediaan herbal. Saat ini banyak sekali sediaan herbal antikanker yang beredar di pasaran, diantaranya sediaan jadi BRM, yang mengandung tansinon, polisakarida, Lycium barbarum, ekstrak Codonopsis pilosulae Radix, dan ekstrak marinealgae. Untuk mengetahui efek sitotoksik sediaan tersebut maka dilakukan uji sitotoksisitas secara in vitro menggunakan biakan sel kanker. Pengujian sitotoksisitas ekstrak etanol dan air sediaan jadi BRM dilakukan terhadap sel Ca Ski (sel kanker serviks) meggunakan metode Uji Sitotoksisitas Ambilan Merah Netral. Dari penelitian ini diperoleh nilai LC50 ekstrak etanol dan air setelah inkubasi 24 jam masing-masing sebesar 274, 16 dan 55,21mg/ml, sedangkan setelah inkubasi 48 jam sebesar 118,58 dan 35,17 mg/ml. Hasil tersebut menunjukkan bahwa ekstrak etanol dan air sediaan jadi BRM mempunyai efek sitotoksik rendah karena memiliki nilai LC50 lebih besar dari 20 mg/ml.
OPTIMASI DAN VALIDASI METODE ANALISIS ASAM NIKOTINAT SERTA STABILITAS INOSITOL HEKSANIKOTINAT Harahap, Yahdiana; Suryadi, Herman; Wardatun, Sri
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 1 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Nicotinic acid is a therapeutic agent for treatment atherosclerosis. Inositol hexanicotinate isan agent that can be hydrolyzed with release nicotinic acid. The low level of free nicotinicacid from inositol hexanicotinate in blood, it’s the reason why it needs method analysis withhigh sensitive and selective. The aims of this research were to optimize and validationmethod analysis nicotinic acid and stability study of inositol hexanicotinate by highperformance liquid chromatography. The method was optimated with variation compositionmobile phase, variation flow rate and optimation process exctraction. Condition analysiswere optimum with use a Kromasil column (250 mm x 4,6 mm) RP, mobile phase mixeddipotassium hydrogen phosphate and potassium dihydrogen phosphate 10 mM containingtetrabuthylammonium bromide 5 mM pH 7 with acetonitril (100:9), flow rate 0,8 ml/minute,with internal standard coffein in 263 nm wave lenght. The standard curve was linear over aconcentration range 124,84 to 5000 ng/ml of nicotinic acid in plasma. The HPLC method wasvalidated with accuracy -6,8779 to 3,09 %, precision 0,3 to 3,71 % and recovery 93,12 -103,09 %. The results of a stability study indicated that inositol hexanicotinate was unstablein plasma samples, but was stable in 0,6 M perchloric acid for to 24 hour at 40C. ABSTRAKAsam nikotinat merupakan obat yang dapat digunakan untuk ateroskerosis dan inositolheksanikotinat merupakan senyawa yang dapat terhidrolisis melepaskan asam nikotinat.Penelitian ini bertujuan untuk optimasi dan validasi metode analisis asam nikotinat, sertamenentukan stabilitas inositol heksanikotinat menggunakan kromatografi cair kinerja tinggi.Kondisi analisis yang optimum diperoleh dengan menggunakan kolom Kromasil (250 mm x4,6 mm) RP, fase gerak campuran kalium dihidrogen fosfat dan dikalium hidrogen fosfat 10mM yang mengandung tetrabutilammonium bromida 5 mM pH 7 dengan asetonitril (100:9),kecepatan alir 0,8 ml/menit, dengan baku dalam kafein, yang dideteksi pada panjanggelombang 263 nm. Kurva kalibrasi linier dari 124,84 sampai 5000 ng/ml. Hasil validasimetode menunjukkan akurasi -6,87 hingga 3,09 %, presisi 0,3 hingga 3,71 % dan perolehankembali 93,12 hingga 103,09%. Hasil uji stabilitas menunjukkan bahwa inositolheksanikotinat tidak stabil dalam plasma tetapi stabil dalam asam perklorat 0,6 M padapenyimpanan 40C selama 24 jam.
UJI SITOTOKSISITAS IN VITRO SEDIAAN JADI BRM (BIOLOGICAL RESPONSE MODIFIER) TERHADAP SEL KANKER SERVIKS Harahap, Yahdiana; Reksodiputro, Harryanto; Heffen, Wan Lelly; Krismartina, Mirna
Jurnal Farmasi Indonesia Vol 3, No 2 (2006)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v3i2.72

Abstract

Cancer was the third big death-caused disease in the developed countries. To overcome the disease, cancer patient can use combinaton of conventional and alternative therapy, such as using herbal   medicine. Nowadays there are many anti cancer herbal medicines in the market, one of which was BRM (Biological Response Modifier), that contain tansinon, polisaccharide, Lycium barbarum, Codonopsis pilosulae Radix extract, and marine algae extract. To investigate the cytotoxic effect of BRM, we  conducted cytotoxicity test in vitro using cancer cell culture. The test examined the activity of ethanol and aquoeus extract of BRM  on Ca Ski cells (cervix cancer cells), using Neutral Red Intake Cytotoxicity Test Method.  Results of the experiment revealed the LC50 of ethanol and aquoeus extract were 274.16 and 55.21mg/ml consecutively, after 24 hour incubation and 118.58 and 35.17 mg/ml after 48 hours incubation. It was concluded that ethanol and aquoeus extract of BRM had low cytotoxicity due to LC50 value greater than 20 mg/ml. ABSTRAK Kanker merupakan penyebab kematian ketiga terbesar di negara berkembang. Untuk mengatasi penyakit tersebut, penderita kanker dapat mengkombinasikan terapi konvensional dengan terapi alternatif, yaitu dengan menggunakan sediaan herbal. Saat ini banyak sekali sediaan herbal antikanker yang beredar di pasaran, diantaranya sediaan jadi BRM, yang mengandung tansinon, polisakarida, Lycium barbarum, ekstrak Codonopsis pilosulae Radix, dan ekstrak marinealgae. Untuk mengetahui efek sitotoksik sediaan tersebut maka dilakukan uji sitotoksisitas secara in vitro menggunakan biakan sel kanker. Pengujian sitotoksisitas ekstrak etanol dan air sediaan jadi BRM dilakukan terhadap sel Ca Ski (sel kanker serviks) meggunakan metode Uji Sitotoksisitas Ambilan Merah Netral. Dari penelitian ini diperoleh nilai LC50 ekstrak etanol dan air setelah inkubasi 24 jam masing-masing sebesar 274, 16 dan 55,21mg/ml, sedangkan setelah inkubasi 48 jam sebesar 118,58 dan 35,17 mg/ml. Hasil tersebut menunjukkan bahwa ekstrak etanol dan air sediaan jadi BRM mempunyai efek sitotoksik rendah karena memiliki nilai LC50 lebih besar dari 20 mg/ml.
UJI SITOTOKSISITAS BUAH MERAH, MAHKOTA DEWA DAN TEMU PUTIH TERHADAP SEL KANKER SERVIKS Radji, Maksum; Aldrat, Hendri; Harahap, Yahdiana; Irawan, Cosphiadi
Jurnal Farmasi Indonesia Vol 5, No 1 (2010)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v5i1.36

Abstract

The cytotoxic effect of herbal medicines has been examined using HeLa cells line (cervical cancer cell culture). The result showed that the LC50 value of buah merah [Pandanus conoideus Lam.], mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.] and temu putih [Curcuma zedoaria (Berg.) Roscoe] extracts were  421 µg/ml, 835µg/ml and 58,9 µg/ml after 24 hour incubation, whereas the LC50 of each extract were 276.79 µg/ml, 415,9 µg/ml and 29.19 µg/ml after 48 hour incubation respectively. The cytotoxic activity of temu putih [Curcuma zedoaria (Berg.)] extract to HeLa cells was stronger than buah merah [Pandanus conoideus Lam.] and mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.] extracts.   ABSTRAK Telah dilakukan penelitian efek sitotoksisitas ekstrak buah merah [Pandanus conoideus Lam.], mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.] and temu putih [Curcuma zedoaria (Berg.) Roscoe] terhadap sel HeLa (kultur sel kanker serviks). Hasil penelitian menunjukkan bahwa nilai LC50 dari ketiga ekstrak tersebut masing-masing adalah 421 µg/ml, 835µg/ml and 58,9 µg/ml, setelah waktu inkubasi selama 24 jam, sedangkan LC50 setelah diinkubasi selama 48 jam adalah  276.79 µg/ml, 415,9 µg/ml and 29.19 µg/ml. Aktivitas sitotoksik  temu putih [Curcuma zedoaria (Berg.)] terhadap sel HeLa  lebih kuat dibandingkan dengan buah merah [Pandanus conoideus Lam.] dan mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.]
OPTIMASI DAN VALIDASI METODE ANALISIS ASAM NIKOTINAT SERTA STABILITAS INOSITOL HEKSANIKOTINAT Harahap, Yahdiana; Suryadi, Herman; Wardatun, Sri
Jurnal Farmasi Indonesia Vol 6, No 1 (2012)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v6i1.94

Abstract

Nicotinic acid is a therapeutic agent for treatment atherosclerosis. Inositol hexanicotinate isan agent that can be hydrolyzed with release nicotinic acid. The low level of free nicotinicacid from inositol hexanicotinate in blood, itâ??s the reason why it needs method analysis withhigh sensitive and selective. The aims of this research were to optimize and validationmethod analysis nicotinic acid and stability study of inositol hexanicotinate by highperformance liquid chromatography. The method was optimated with variation compositionmobile phase, variation flow rate and optimation process exctraction. Condition analysiswere optimum with use a Kromasil column (250 mm x 4,6 mm) RP, mobile phase mixeddipotassium hydrogen phosphate and potassium dihydrogen phosphate 10 mM containingtetrabuthylammonium bromide 5 mM pH 7 with acetonitril (100:9), flow rate 0,8 ml/minute,with internal standard coffein in 263 nm wave lenght. The standard curve was linear over aconcentration range 124,84 to 5000 ng/ml of nicotinic acid in plasma. The HPLC method wasvalidated with accuracy -6,8779 to 3,09 %, precision 0,3 to 3,71 % and recovery 93,12 -103,09 %. The results of a stability study indicated that inositol hexanicotinate was unstablein plasma samples, but was stable in 0,6 M perchloric acid for to 24 hour at 40C. ABSTRAKAsam nikotinat merupakan obat yang dapat digunakan untuk ateroskerosis dan inositolheksanikotinat merupakan senyawa yang dapat terhidrolisis melepaskan asam nikotinat.Penelitian ini bertujuan untuk optimasi dan validasi metode analisis asam nikotinat, sertamenentukan stabilitas inositol heksanikotinat menggunakan kromatografi cair kinerja tinggi.Kondisi analisis yang optimum diperoleh dengan menggunakan kolom Kromasil (250 mm x4,6 mm) RP, fase gerak campuran kalium dihidrogen fosfat dan dikalium hidrogen fosfat 10mM yang mengandung tetrabutilammonium bromida 5 mM pH 7 dengan asetonitril (100:9),kecepatan alir 0,8 ml/menit, dengan baku dalam kafein, yang dideteksi pada panjanggelombang 263 nm. Kurva kalibrasi linier dari 124,84 sampai 5000 ng/ml. Hasil validasimetode menunjukkan akurasi -6,87 hingga 3,09 %, presisi 0,3 hingga 3,71 % dan perolehankembali 93,12 hingga 103,09%. Hasil uji stabilitas menunjukkan bahwa inositolheksanikotinat tidak stabil dalam plasma tetapi stabil dalam asam perklorat 0,6 M padapenyimpanan 40C selama 24 jam.
Optimasi Penetapan Kadar Akrilamida Yang Ditambahkan Ke Dalam Keripik Kentang Simulasi Secara Kromatografi Cair Kinerja Tinggi Harahap, Yahdiana; Harmita, Harmita; Simanjuntak, Binsar
Majalah Ilmu Kefarmasian Vol. 2, No. 3
Publisher : UI Scholars Hub

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Abstract

A method by high performance liquid chromatography for the analysis of acrylamide in potato chips, is reported. The retention time for the elution of acrylamide from the C18RP column ranged from 3 to 3,2 minutes, and the eluate was analyzed by UV-VIS detector. A linear response was found for the acrylamide standard tested within the concentration range of 0,8 – 10µg/ml and the corelation coefficient (r) greater than 0,999, with detection limit 0,06 ppm and quantitative limit 0,19 ppm. Sample preparation was performed by means of solvent extraction using dichlormethane and subsequent re-extraction of the organic solvent with water. This aqueous sample solution was found to be free of any interferences and gave acrylamide and recorveries higher than 90%.
Analisis Glimepirida Dalam Plasma Tikus Harahap, Yahdiana; Mansur, Umar; Sinandang, Theresia
Majalah Ilmu Kefarmasian
Publisher : UI Scholars Hub

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Abstract

The aim of this research is to find the method for analyze glimepiride and itÂ’s metabolite. Glimepiride is the second generation of antidiabetic oral from the sulphonyl urea that works by stimulating the insulin secretion from beta cells of pancreas. Glimepiride is isolated from plasma the using chloroform. Using the high performance liquid chromatography method which include C18 reversed phase column, using mixture of methanol:water (50:50, v/v) as a mobile phase, flow rate 1.0 ml/minutes, detection at wavelenght 228 nm with photo diode array detector gives retention times of glimepiride in 17 minutes without any interference from endogen component of plasma and from itÂ’s metabolite. Linearity with added internal standard gliclazide was established for the range concentration 100-1000 ng/ml with coefficient of correlation (r) is 0.9977 and give the limit of quantitation of glimepiride in 50 ng/ml. The results of validation method fulfilled for the given criterias.
Pembentukan Akrilamida Dalam Makanan Dan Analisisnya Harahap, Yahdiana
Majalah Ilmu Kefarmasian
Publisher : UI Scholars Hub

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Abstract

Acrylamide is a chemical substance which derived from acrylonitrile, is the material used in polyacrylamide production. Recent research has found acrylamide is contained in some food, especially food is rich in carbohydrate and treated in high temperature (more than 120°C). Due to its nature, acrylamide is classified as a hazardous material to be contained in human’s food. The International Agency for Research on Cancer (IARC) has classified acrylamide into group 2A (probably carcinogenic for humans). Many methods that used to analyse the acrylamide in some foods with sophisticated equipment, and in department of pharmacy FMIPA-UI there were also develop the method with simple extraction and conventional HPLC
Validasi Metode Analisis Cilostazol Dalam Plasma In Vitro Secara Kromatografi Cair Kinerja Tinggi Harahap, Yahdiana; Mansur, Umar; Estherina, Christine
Majalah Ilmu Kefarmasian Vol. 5, No. 1
Publisher : UI Scholars Hub

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Cilostazol is an antiplatelet agent with the mechanism of action by inhibiting phos-phodiesterase III (PDE III). Referred to Food and Drug Administration(FDA),cilostazol is a drug recommended to be bioequivalence (BE) studied. A high-perfor-mance liquid chromatographic (HPLC) method with ultraviolet detector for in vitro determination of cilostazol in human plasma had been developed and validated. Cilostazol and pioglitazone as internal standard were extracted from human plasma by protein precipitation method using methanol. The mobile phase consisting of ac-etonitrile-potassium di-hydrogen phosphate buffer 50 mM (40:60) was used at the flow rate of 1.5 mL/min on reversed phase C18 column (SunfireTM, 5 µm, 250x4.6 mm), and was detected at wavelength of 257 nm. Linearity was established within concentration range of 20-2000 ng/mL with coefficient correlation (r) was 0,9999. Accuracy (% diff) of this method was -14.67% up to 8.84% with precision (CV) being 0.98% to 4.93%, and absolute recovery was established to be 82.26% to 119.85%. Cilostazol in plasma was stable for 30 days in -200C storage.
Co-Authors . Harmita . Hayun . Hayun Aldrat, Hendri Aldrat, Hendri Ani Susanti Ani Susanti Bambang Karsono Binsar Simanjuntak Binsar Simanjuntak, Binsar Christine Estherina Christine Estherina, Christine Citra Nur Azizah Citra Nur Azizah, Citra Nur Cosphiadi Irawan Dadang Kusmana Erilia Kesumahati Harmita Harmita Harmita Harmita, Harmita Harryanto Reksodiputro, Harryanto Hayun Hayun Hayun Hayun, Hayun Heffen, Wan Lelly Heffen, Wan Lelly Herman Suryadi Indah Widyaningsih Krismartina, Mirna Krismartina, Mirna Letitia Tania, Letitia Loedfiasfiati Alawiyah Maksum Radji Maryati Kurniadi Mun'im, Abdul Nadia Farhanah Syafhan Nasution, Azlaini Yus Noorwati Sutandyo Nu Alia Nu Alia, Nu Rahmania, Tesia Aisyah Renesteen, Editha Saputri, Fadlina Chany, Dr. Sherly Meilianti Sherly Meilianti, Sherly Sitepu, Eme Stepani Sri Wardatun Theresia Sinandang Theresia Sinandang, Theresia Umar Mansur Umar Mansur Umar Mansur, Umar Wan Lelly H Wardhani, Bantari Wisynu Kusuma