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CULTIVATION CONDITIONS FOR PROTEASE PRODUCTION BY A THERMO-HALOSTABLE BACTERIAL ISOLATE PLS A Teuku M. Iqbalsyah; Malahayati Malahayati; Atikah Atikah; Febriani Febriani
Jurnal Natural Volume 19, Number 1, February 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.586 KB) | DOI: 10.24815/jn.v19i1.11971

Abstract

Polyextremophiles have increasingly been utilised to produce thermostable enzymes with better stability in multiple extreme conditions. This study reports the screening results of four new bacterial isolates (PLS A, PLS 75, PLS 76 and PLS 80), isolated from an under water hot springs, in producing thermo-halostable protease enzyme. Optimum cultivation conditions for the protease production were also studied. Screening of protease-producing isolates was conducted using Thermus solid medium enriched with 3% skim milk and 0.5% casein. The growth of the isolates showing protease activity was monitored by measuring the cell dry weight and protease activity during 24 h cultivation period. The activity was also measured at various cultivation conditions, i.e. temperature, pH and salt concentrations. Amongst the four isolates, only PLS A showed the ability to produce protease. The optimum cultivation conditions for protease production were observed at 65°C, pH 7 for 18 h incubation. The activity increased with the addition of 1% NaCl concentration (0.085 Unit/mL). The ability of PLS A isolate to produce thermo-halostable protease was encouraging as they could potentially be used in industries requiring the enzyme with multiple extremes. 
Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain Iqbalsyah, Teuku Mohamad; Velayati, Muhammad A; Helwati, Hira; Febriani, Febriani
Jurnal Kimia Sains dan Aplikasi Vol 23, No 5 (2020): Volume 23 Issue 5 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2315.333 KB) | DOI: 10.14710/jksa.23.5.183-188

Abstract

The search for novel DNA Polymerases I, with higher fidelity and better polymerization rate, is essential to improve the Polymerase Chain Reaction method. A thermo-halophilic bacterium has been isolated from an undersea hot spring, dubbed Pria Laot Sabang (PLS) A strain. The 0.9 kb DNA Polymerase I gene fragments from the isolate were amplified, sequenced, and identified. The fragments were part of the polymerization domain of the enzyme. Homological analysis of the gene sequence showed that the PLS A strain was closely related to Bacillus caldolyticusstrain XM. However, Swissprot structural analysis reveals that PLS A strain had high homology to Geobacillus stearathermophilus. Full sequence analysis is still needed to identify the species and evaluate the intact enzyme structure.
Purification and Partial Characterization of α-Amylase Produced by a Thermo-Halophilic Bacterium Isolate PLS 75 Fajarna, Farah; Iqbalsyah, Teuku M.; Febriani, Febriani
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.111 KB) | DOI: 10.15294/biosaintifika.v10i3.15861

Abstract

Bio-based industries require stable enzymes in a broad range of environmental conditions. Extremophiles have attracted more interests as the source of such enzymes, one of which is α-amylase. This study aimed to purify and characterize α-amylase produced by a thermo-halophilic bacterium PLS 75 isolated from underwater fumaroles. Ammonium sulfate precipitation results showed that the highest specific α-amylase activity (21.7 U/mg) obtained at 40-60% saturation level, with a purity of 7.7-fold of the crude extract with 16.2% yield. Further purification using DEAE Sepharose column chromatography increased the enzyme purity 11.1-fold of the crude extract with 7.1% yield. Specific activity after column chromatography purification was 31.3 U/mg. The pure enzyme had a low molecular weight of 14 kDa. The enzyme showed the highest activity at 80 °C and pH 5. The activity increased to 126% when in methanol, while decreased when in ethyl acetate and chloroform. The characteristics of α-amylase with low molecular weight, which was active in acidic condition, stable in polar and non-polar solvents, may be used for for specific industrial needs.
KORELASI TEKANAN PENCETAKAN TERHADAP KARAKTERISTIK BRIKET DENGAN VARIASI LUBANG Muhammad Hidayat; M Iqbalsyah; Syarifah Nurul Carissa; Zahra Fona; Adriana Adriana
Jurnal Sains dan Teknologi Reaksi Vol 17, No 2 (2019): JURNAL SAINS DAN TEKNOLOGI REAKSI
Publisher : Politeknik Negeri Lhokseumawe

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30811/jstr.v17i2.1484

Abstract

Penelitian ini bertujuan untuk melihat pengaruh tekanan pencetakan terhadap karakteristik briket dengan variasi lubang Perlakuan Jumlah lubang: 0, 1, 2, dan 3 lubang dan waktu Pengepresan: 30, 45, 60, dan 75 detik. Dari perlakuan tersebut diketahui bahwa waktu optimum untuk pengepresan briket adalah 5 menit.hal ini terjadi apabila semakin lama waktu pengerpresan maka briket akan hancur dan tidak terbentuk. Nilai kalor tertinggi didapatkan pada perbandingan tekanan sebesar 30 detik dengan bahan baku tempurung kelapa dengan penggunaan perekat kanji sebanyak 8%. Nilai kalor yang didapatkan sebesar 6858 kal/gr. Jadi nilai kalor yang didapat telah memenuhi standar minimum nilai kalor yang ditentukan SNI.
Purification and Partial Characterization of α-Amylase Produced by a Thermo-Halophilic Bacterium Isolate PLS 75 Iqbalsyah, Teuku M.; Fajarna, Farah; Febriani, Febriani
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.15861

Abstract

Bio-based industries require stable enzymes in a broad range of environmental conditions. Extremophiles have attracted more interests as the source of such enzymes, one of which is α-amylase. This study aimed to purify and characterize α-amylase produced by a thermo-halophilic bacterium PLS 75 isolated from underwater fumaroles. Ammonium sulfate precipitation results showed that the highest specific α-amylase activity (21.7 U/mg) obtained at 40-60% saturation level, with a purity of 7.7-fold of the crude extract with 16.2% yield. Further purification using DEAE Sepharose column chromatography increased the enzyme purity 11.1-fold of the crude extract with 7.1% yield. Specific activity after column chromatography purification was 31.3 U/mg. The pure enzyme had a low molecular weight of 14 kDa. The enzyme showed the highest activity at 80 °C and pH 5. The activity increased to 126% when in methanol, while decreased when in ethyl acetate and chloroform. The characteristics of α-amylase with low molecular weight, which was active in acidic condition, stable in polar and non-polar solvents, may be used for for specific industrial needs.
ADDITION OF POTENTIAL PROBIOTIC BACTERIA TO INCREASE GROWTH PERFORMANCE AND FEED EFFICIENCY OF COMMON CARP (Cyprinus carpio) Cut Yulvizar; Irma Dewiyanti; Cut Nanda Defira; T.M. Iqbalsyah; Suhartono Suhartono
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.5458

Abstract

Three intestinal bacteria isolates (namely C1, C2, and C3) from common carp (Cyprinus carpio) were isolated and identified as new potential probiotics. This research aimed to study the effect of three isolates potential probiotics addition on growth performance, survival rate and feed efficiency of common carp. Completely randomized design was assigned to fourth diet treatments with five replicates. As a control, we used the feed without any supplemention of potential probiotic. The result showed that the addition of potential probiotic bacteria (C1, C2, and C3) gave significant effect on daily growth rate, survival rate and feed efficiency and could be used in aquaculture industry to reduce feeding cost.
NUTRITIONAL COMPOSITION OF DIOSCOREA HISPIDA FROM DIFFERENT LOCATIONS AROUND LEUSER ECOSYSTEM AREA Sitti Saleha; Nurdin Saidi; Saiful .; Murniana .; Saida Rasnovi; Teuku M. Iqbalsyah
Jurnal Natural Volume 18, Number 1, February 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.348 KB) | DOI: 10.24815/jn.v18i1.8504

Abstract

Proximate analysis of Dioscorea hispida tubers, collected from five locations around Leuser ecosystem in Aceh Province, showed variations amongst samples. Standard AOAC method for proximate analysis of the fresh weight showed that the water content varied between 15.8 - 37.8%, crude protein 1.13 -6.20%, crude lipid 1.99 - 9.36% and ash 0.29 - 1.24%. The total carbohydrate was high, i.e. between 58.3 -71.9%. The main mineral was phosphorus, with a value of 11.7 - 46.9 mg/100g. These variations could be due to soil, climate and weather factors, as well as postharvest handling. Phytochemical tests showed that all of the samples contained alkaloids and terpenoids. One of the samples (LP) also contained phenol and steroid. The high cyanide content in the tubers (379 - 739 ppm) was easily removed by repeated washing. The cyanide level dropped significantly after the 3rd wash. Information on nutritional content in D. hispida is essential for planning its utilization. Increasing the economic value of D. hispida is expected to attract people around the Leuser ecosystem to cultivate and utilize it, thereby reducing illegal forest encroachment.Keywords: Dioscorea hispida, proximate, Leuser, janeng, gadung, starchREFERENCESBarton H 2014 Yams: Origins and Development, Encyclopaedia of Global Archaeology, p 7943-7947, (Springer. DOI 10.1007/978-1-4419-0465-2_2193).Obidiegwu J E and Akpabio E M 2017 The Geography of Yam Cultivation in Southern Nigeria: Exploring Its Social Meanings and Cultural Functions J. Ethnic Foods 4 28-35.Chandrasekara A and Kumar T J 2016 Roots and Tuber Crops as Functional Foods: A Review on Phytochemical Constituents and Their Potential Health Benefits Intl. J. Food Sci. 2016 1-15.Kumar S, Das G, Shin H-S and Patra J K 2017 Dioscorea spp. (A Wild Edible Tuber): A Study on Its Ethnopharmacological Potential and Traditional Use by the Local People of Similipal Biosphere Reserve India Front. Pharmacol. 8 52.Lin J Y, Lu S, Liou Y L and Liou H L 2006a Antioxidant and Hypolipidaemic Effects of a Novel Yam–boxthorn Noodle in an In Vivo Murine Model Food Chem. 94 377–384.Lin J Y, Lu S, Liou Y L and Liou H L, 2006b, Increased IgA and IgG Serum Levels Using a Novel Yam–boxthorn Noodle in a BALB/c Mouse Model Food Chem. Toxicol. 44 170–178.Bhandari M R and Kawabata J 2004 Organic Acid, Phenolic Content and Antioxidant Activity of Wild Yam (Dioscorea spp.) Tubers of Nepal Food Chem. 88 163–168.Lin J T and Yang D J 2008 Determination of Steroidal Saponins in Different Organs of Yam (Dioscorea pseudojaponica Yamamoto) Food Chem. 108 1068–1074.AOAC 1984. Official Methods of Analysis. Washington DC: Association of Official Analytical Chemists.Harborne J B 1984 Phytochemical Method 2nd ed. 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Food Eng. 61 483–495Yeh A-I, Chan T-Y and Chuang G C-C 2009 Effect of Water Content and Mucilage on Physico-chemical Characteristics of Yam (Discorea alata Purpurea) Starch J. Food Eng. 95 106–114.McPherson E and Jane J 1999 Comparison of Waxy Potato with Other Root And Tuber Starches Carbohydr. Polym. 40 57–70.Freitas R A, Paula R C, Feitosa J P A, Rocha S and Sierakowski M R 2004 Amylose Contents, Rheological Properties and Gelatinization Kinetics of Yam (Dioscorea alata) and cassava (Manihot utilissima) starches Carbohydr. Polym. 55 3–8.Barsby T L, Donald A M, Frazier P J, Donald A M, Perry P A and Waigh T A 2001 The Impact of Internal Granule Structure on Processing and Properties in Starch: Advances in Structure and Function p 45-52 (Royal Society of Chemistry, http://dx.doi.org/10.1039/9781847551917-00045)Tattiyakul J, Naksriarporn T and Pradipasena P 2012 X-ray Diffraction Pattern and Functional Properties of Dioscorea hispida Dennst Starch Hydrothermally Modified at Different Temperatures Food Bioproc. Technol. 5 964–971.Savikin-Fodulovic K, Grubisic D, Culafic L, Menkovic N and Ristic M 1998 Diosgenin and Phytosterols Content in Five Callus Lines of Dioscorea balcanica Plant Sci. 135 63–67.Cushnie T. P. T, Cushnie B and Lamb A J 2014 Alkaloids: An Overview of Their Antibacterial, Antibiotic-enhancing and Antivirulence Activities Int. J. Antimicrob. Agents 44 (5) 377-386.Tholl D 2015 Biosynthesis and Biological Functions of Terpenoids in Plants, Biotechnology of Isoprenoids p 63-106 Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 148).Nagata K, Aistrup G L, Honda H, Shono T and Narahashi T 1999 Modulation of the Nicotinic Acetylcholine Receptor by Dioscorine in Clonal Rat Phaeochlomocytoma (PC12) Cells Pestic. Biochem. Physiol. 64 (3) 157–165.Bhandari M R and Kawabata J 2005 Bitterness and Toxicity in Wild Yam (Dioscorea spp.) Tubers of Nepal Plant Foods Hum. Nutr. 60 129–135, 2005.White W L B , Arias-Garzon D I, McMahon J M and Sayre R 1998 Cyanogenesis in Cassava: The Role of Hydroxynitrile Lyase in Root Cyanide Production Plant Physiol. 116 1219-25.Kumoro A C and Hartati I. 2015 Microwave Assisted Extraction of Dioscorin from Gadung (Dioscorea hispida Dennst) Tuber Flour, Procedia Chem. 14 47 – 55.
PHYLOGENETIC ANALYSIS OF 16S rRNA GENE FRAGMENT OF ANTIBIOTIC-PRODUCING PLS 76 ISOLATE Mulia Aria Suzanni; Amelya Yolanda; Nurdin Saidi; Febriani Febriani; Teuku Mohamad Iqbalsyah
Jurnal Natural Volume 18, Number 3, October 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v18i3.11152

Abstract

Exploitation of extremophiles as novel bioactive compounds sources has been increasing. The aim of this study was to evaluate the activity and the class of antibiotic produced by a thermo-halophilic isolate PLS 76, as well as to identify the genotype of the isolate. The activity was determined by a disc diffusion method, while the antibiotic class was determined qualitatively by chemical reactions using ninhydrin, iodine vapour and potassium iodine. The genotype was determined by sequencing the 16S rRNA gene fragment and the phylogenetic tree from the sequence data. The results showed that PLS 76 was a Gram-negative bacterium and able to produce polypeptide antibiotic, which showed a slight activity on E. coli and S. aureus. Sequence alignment of the 16S rRNA gene fragment showed that PLS 76 was most related to Geobacillus kaustophilus. These results may be used to utilise the isolated for further antibiotic study.