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All Journal Jurnal Sain Veteriner Buletin Veteriner Udayana Jurnal Veteriner BERITA BIOLOGI Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Prosiding Seminar Nasional Unimus

Wisnu Nurcahyo

Unknown Affiliation

Author-ID : 2760486

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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STUDI RESPON IMUN PADA AYAM YANG DIIMUNISASI DENGAN ANTIGEN EKSKRESI SEKRESI SPOROZOIT Eimera tenellla Joko Prastowo; Wisnu Nurcahyo; Kurniasih .; R. Wasito .
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Pengaruh Infeksi Toxoplasma gondii Isolat Lokal terhadap Gambaran Darah pada MEncit (Mus musculus) Wisnu Nurcahyo; Dwi Priyo Widodo; M. Hanafiah
Jurnal Sain Veteriner Vol 22, No 1 (2004): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

STUDI EKSPERIMENTAL SISTA JARINGAN Toxoplasma gondii SECARA IN VIVO AN EXPERIMENTAL STUDY OF Toxoplasma gondii TISSUE CYST IN VIVO M.Hanafiah .; Wisnu Nurcahyo; Sumartono .
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Produksi Antibodi Anti-Dirofilaria immitis Untuk Pengembangan Diagnosis Dirofilariasis Pada Anjing I Gusti Made Krisna Erawan; Ida Tjahajati; Wisnu Nurcahyo; Widya Asmara
Buletin Veteriner Udayana Vol. 8 No. 2 Agustus 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Dirofilaria immitis (D. immitis) as a causative agent of heartworm disease is not only caused problems in animals but also zoonoses. For diagnosis of dirofilariasis (disease caused by D. immitis) serologically is needed anti-D. immitis antibodies. The objective of this study was to produce antibodies against excretory-secretory antigens produced by male and female worms for developing the diagnosis of dirofilariasis based on the antigens detection. Base on this study can be concluded that male excretory-secretory antigens (MES), female excretory-secretory antigens (FES), and MES+FES can stimulate BALB/c mouse to produce polyclonal antibodies in the same pattern. Antibodies have been produced at day 21 and the peak titter was at day 35 after first immunization.

Penggunaan Protein Membran Stadium Bradizoit Toxoplasma gondii untuk Mendiagnosis Toksoplasmosis dengan Metode Intradermal Test Muhammad Hanafiah; Wisnu Nurcahyo; Dwinna Aliza; Teuku Fadrial Karmil
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A research was conducted to find out an alternative diagnose in detecting toxoplasmosis in livestock/animal using intradermal test from protein membrane of T. gondii bradizoite stage. Local isolate ofmembrane T. gondii bradizoite stage was used in the research. Ten of domestic sheep with the age of ± 1year and 10 mice strain Balb/c with the age ± 2 month were used in this research. The reaction ofhypersensitivity on the skin post protein membrane bradyzoite injection was indicated by the process ofskin thickening. The diameter skin thickening was measured using cutimeter, in which diameter e” 10 mmindicated positive diagnose. The result showed that optimal dosage of membran protein bradyzoite thatcould be applied to detect toxoplasmosis in livestock and animal using intradermal test were 0,6 ml and0,2 ml for sheep and mice respectively. The sensitivity and specificity level of antigen use (protein membrane)of T. gondii bradizoite stage from local isolate to diagnose toxoplasmosis in mice using intradermal testwere: 85.0 % and 66.6 % respectively, while in sheep the sensitivity and specificity level were 85.0 % and66.6 % respectively. It can be concluded that intradermal test was appropriate to be implemented fordetecting toxoplasmosis in sheep and mice induced with tachyzoite T. gondii.

Molecular Detection of Toxoplasmosis Using Specific Primers P30, B1, and rDNA Wisnu Nurcahyo; Joko Prastowo; Ana Sahara
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Study in order to develop molecular techniques using specific primers for the early diagnosis oftoxoplasmosis have been conducted. Detection of Toxoplasma gondii genome was performed usingpolymerase chain reaction (PCR) technique. The primers used in this study were rDNA, P30, and B1. ThePCR products were further run using gel electrophoresis (gel 1.5% – 2.0%) and the band was documented.Toxoplasma was detected at 500 bp and 600 bp using primer P30 and B1, respectively. Whereas usingprimer rDNA no band was observed. It was assumed that primer rDNA was not sensitive since the targetamplification was 88 bp.

Daya Larvasida Ekstrak Daun Muda Kedondong Hutan Terhadap Haemonchus contortus Secara In-vitro (LARVICIDAL EFFECT OF SPONDIAS PINNATA LEAF EXTRACT AGAINST HAEMONCHUS CONTORTUS IN VITRO) I Gusti Komang Oka Wirawan; Wisnu Nurcahyo; Joko Prastowo; Kurniasih Kurniasih
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aimed to asses effective concentration of condensed tannins in the extract of Lannea grandis leaves (EDMKH) as larvacide anti-helminths on Haemonchus contortus. Experimental design used in this study was completely randomised design. The treatments group were administrated with 2.5%, 3.5% , and 4.5% EDMKH in the 0.2 g/mL solution, respectively. Moreover aqueduct was used for the negative control group, while 0.055% albendazole was applied to the positive control group. Variables measured were percentage of developed and undeveloped L1 in the incubation media. The data were the statistically analysed following General Linear Model format for completely randomised design. The software used in the analysis was SPSS v. 23.0 (IBM-SPSS 2015). Results showed that among the EDMKH treatments, the lowest larvacidal effect was recorded for 2.5% EDMKH treatment compared with 3.5% and 4.5% EDMKH. However, the value achieved by 2.5% EDMKH treatment was significantly higher (P<0.05) than that of negative control treatment. The highest larvacidal effect (100%) was shown by 4.5% EDMKH treatment which was not significantly different (P>0.05) from the positive control treatment. ABSTRAK Penelitian ini bertujuan untuk mengetahui konsentrasi efektif tanin kondensasi di dalam Ekstrak Daun Muda Kedondong Hutan (EDMKH) sebagai antelmintik yang bersifat larvasida terhadap Haemonchus contortus (H. contortus) secara in-vitro. Rancangan penelitian yang digunakan dalam penelitiam ini adalah Rancangan Acak Lengkap (RAL), dengan lima perlakuan yaitu tiga perlakuan EDMKH konsentrasi: 2,5%, 3,5%, 4,5% dari 0,2 g/mL larutan tersedia, satu kelompok kontrol (-) menggunakan air suling, satu kelompok kontrol (+) menggunakan albendazole konsentrasi 0,055%. Variabel yang diukur dan dianalisis: jumlah persentase L1 yang berkembang dan tidak berkembang dalam kelompok rendaman. Data persentase daya larvasida H. contortus dari konsentrasi tanin kondensasi EDMKH secara in-vitro dianalisis menggunakan analisis varian mengikuti prosedur Rancangan Acak Lengkap (RAL) menggunakan SPSS Versi 23(IBM-SPSS 2015). Hasil analisis statistika menunjukkan bahwa persentase larvasida EDMKH 2,5% nyata lebih rendah dibandingkan 3,5%, 4,5% dan albendazole 0,055% (P<0,05) tetapi lebih tinggi dari kontrol negatif. Konsentrasi efektif EDMKH terhadap larva H. contortus adalah konsentrasi 4,5%, memberikan efektivitas larvasida 100%, dan tidak berbeda nyata dengan kontrol positif (P>0,05).

Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) Galuh Tresnani; Joko Prastowo; Wisnu Nurcahyo; Budi Setiadi Daryono
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The research on developing diagnostic method, vaccine, and drugs for coccidiosis has been focused onthe finding of the immunogenic molecule in Eimeria. The identification of this agent will need the antibodywhich can recognize the biomolecule in the antigen. Antibody that has been developed for this purposeshould be analyzed first, and one of the simple methods for analyzing this antibody is through dot blotanalysis. The objective of this research was to analyze the polyclonal antibody which developed from theoocyst, sporocyst, and sporozoite of E. tenella using dot blot analysis. The antigen for this polyclonalantibody was made from each of the E. tenella stadium by sonication. Fifteen mice, divided into 3 groups,were then injected subcutaneously with each antigen. The sera from these mice were then collected, analyzedby using ELISA and then it will be used for the dot blot analysis. The research result showed that thepolyclonal antibody which has been developed in mice from each antigen can react with the antigen itself.From this result it can be concluded that the developing of this antibody is successful and it can be used forfurther research in immunoproteomic.

Kekerabatan Genetik Caplak Rhiphicephalus (Boophilus) microplus Asal IndonesiaBerdasarkan Sekuen Internal Transcribed Spacer-2 (GENETIC RELATIONSHIP INDONESIAN RHIPHICEPHALUS (BOOPHILUS) MICROPLUS TICK BASED ON INTERNAL TRANSCRIBED SPACER-2 SEQUENSE ) Ana Sahara; Joko Prastowo; Rini Widayanti; Kurniasih .; Wisnu Nurcahyo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Rhiphicephalus (Boophilus) microplus is important obligatory blood feeding ectoparasites transmittingmany different viral, bacterial and protozoan and plays a role as a vector of Babesia sp., The leria sp. andAnaplasma sp. in cattle. The accuracy in identifying and distinguishing interspecies and intraspeciesdiversity among parasites is needed to understand the epidemiology, biology and capacity as a vector.Variations in the DNA base sequence of the internal transcribed spacer region2 (ITS 2) has been used asa molecular marker for identification in an effort to determine phylogenetic relationships. The aim of thisstudy was to determine the ITS 2 gene nucleotide sequence of R. microplus, which was expected to beuseful for accurate identification the parasite diversity and phylogenetic relationship among many differentspecies. DNA amplification was conducted using BOO2 forward dan BOO2 reverse primers. The DNAsamples containing ITS2 region fragment of 1099 nt were derived from the nucleotide sequence multiplealignments of R.microplus and other ticks genes obtained from Gene bank using Clustal W software, andthen analyzed using the MEGA program version 6. Genetic distances based on nucleotide sequence weredetermined with Kimura 2-parameter method producing the smallest genetic distance of 0 % and 1.2 %.Construction of phylogenetic trees using the Neighbor joining method showed that ticks from variousregions in Indonesia was species complex which have a closer with R.microplus isolates from India, Laos,South Africa, China and Australia R.australis origin.

Produksi dan Isolasi Protein Membran Stadium Bradizoit Toxoplasma gondii : Suatu Usaha untuk Mendapatkan Material Diagnostik dalam Mendiagnosa Toksoplasmo Muhammad Hanafiah; Wisnu Nurcahyo; Mufti Kamaruddin; Fadrial Karmil
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study was conducted to isolate the membrane protein of Toxoplasma gondii at bradyzoid stage forthe development of intradermal diagnostic test in livestock infected by the parasite. Toxoplasma wasinitially collected from meat of goat serologically positive to the parasite. The infected meat was then fedinto uninfected cat to obtain oocyst. The oocyst was inoculated into the stomach of mice to produce tachyzoitwhich in turn produce cyst in tissue known as bradyzoit . The membrane protein was then isolated from thebradyzoit. The protein was analysed by sodium dodecyl sulfate electrophoresis (SDS-PAGE). The dataobtained were presented descriptively. The protein concentration isolated from each mouse infected at thedose of 1x107 oocysts was 11.91 mg. Two protein bands specific for bradyzoit were identified at 97.72 kDaand 67.60 kDa.

Co-Authors Ana Sahara Ana Sahara BUDI SETIADI DARYONO Dikry N Shatrie Dwi Priyo Widodo Dwinna Aliza Erdiansyah Rahmi Erdiansyah Rahmi Fadrial Karmil Galuh Tresnani I Gusti Komang Oka Wirawan I Gusti Made Krisna Erawan Ida Tjahajati Joko Prastowo Kurniasih . Kurniasih Imamudin Kurniasih Kurniasih Luluk Ria Rakhma M. Hanafiah M.Hanafiah . Muchlison Anis Mufti Kamaruddin Muhammad Hanafiah Muhammad Hanafiah R. Wasito . Rini Widayanti Sri Hartati Sumartono . Teuku Fadrial Karmil Triyanto Triyanto Widya Asmara

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