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Proximate Analysis of Protein and Relative Growth of Tilapia Fish Exposed to Heat Stress and Fed with the Combination of Willow Leaf and Chromium Ari Widodo; muhammad isa; teuku armansyah TR
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

This study was aimed to determine the effect of relative growth rate and proximate levels of protein in tilapia fish exposed to heat stress and supplemented with the combination of willow leaf powder and chromium. A total of 30 tilapia fish with 1.3-1.5 cm long were divided into 6 treatment groups with 5 repetitions: fed with commercial feed at temperature 29±1° C (P1S1), fed with commercial feed combined with willow leaf at temperature 29±1° C (P2S1), fed with commercial feed combined with willow leaf and chromium at temperature 29±1° C (P3S1), fed with commercial feed at temperature 35±1° C (P1S2), fed with commercial feed combined with willow leaf at temperature 35±1° C (P2S2), fed with commercial feed combined with willow leaf and chromium at temperature 35±1° C (P3S2). The treatments were carried out for 15 days. Then, relative growth rate and proximate levels of proteins were calculated using Kjedahl methods. The results showed that there was an increase of the mean of relative growth rate and proximate levels of protein. Based on statistical test using factorial completely randomized design it was showed that there was a significant effect (P0.01) on the interaction of feeding and temperature with the relative growth rate, but did not affect the relative growth rate of tilapia fish. The proximate level of tilapia fish protein was affected very significantly (P0.01) by the food and the interaction between food and temperature as well, while the temperature treatment gave no significant effect on the proximate level of tilapia fish protein. In conclusion, the commercial feed combined with willow leaf and chromium can increase the relative growth rate and the proximate level of protein of tilapia fish exposed to heat stress.Key words: relative growth rate, proximate protein, willow leaf, chromium, tilapia fish

Description of Depik Fish (Rasbora Tawarensis) Lipid Content in Laut Tawar Lake Aceh Tengah Syahrin Novia; muhammad isa; Razali Razali
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

This study was aims to determine the fat content of depik fish (Rasbora tawarensis) collected from Laut Tawar Lake Central Aceh. The samples used were fresh fish from traditional catching around Laut Tawar lake with the weight of 12.45 and 12.74 gram. The samples were analyzed by Soxhlet method with two repetitions. The result of this study showed that the fat content of depik fish was 6,50%. It can be concluded that depik fish from Laut Tawar lake Central Aceh had average of fat content of 6,50%.Key words: Rasbora tawarensis, Laut Tawar, Aceh Tengah

ANALISIS KADAR PROTEIN IKAN DEPIK (Rasbora tawarensis) DI DANAU LAUT TAWAR KABUPATEN ACEH TENGAH (Protein Content Analysis of Depik (Rasbora tawarensis) In Laut Tawar Lake Aceh Tengah) Iskandar Munthe; Muhammad Isa; Winaruddin Winaruddin; Sulasmi Sulasmi; Herrialfian Herrialfian; Rusli Rusli
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

This study aimed to determine protein content of depik (Rasbora tawarensis) fish collected from fish culture in Laut Tawar Lake, Aceh Tengah. Protein content in fish was analyzed using Kjehdal method with two replications. The result showed that average protein content of depik fish was 15.29%. Based on the result, it could be concluded that protein content of fish depik from Laut Tawar Lake is 15.29%.

ISOLASI DAN IDENTIFIKASI VIRUS AVIAN INFLUENZA PADA BERBAGAI SPESIES UNGGAS SECARA SEROLOGIS DAN MOLEKULER (Isolation and Identification of Avian Influenza in Different Species of Poultry by Means of Serological and Molecular Methods) Teuku Zahrial Helmi; Charles Rangga Tabbu; Wayan Tunas Artama; Aris Haryanto; Muhammad Isa
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

The purpose of this research was to identify avian influenza (AI) virus using serological and molecular methods on poultry which suspected as AI infected in Aceh province. This study used 37 samples of tracheal and cloacal swabs and organs from various species of poultry that were collected from several districts/cities in Aceh. Samples were collected and put into transport media and stored at 4° C before sending to the laboratory. Samples were inoculated in specific pathogen-free of embryonated chicken egg with the age of 9-11 days for further serological and molecular examination. From 37 samples which infected to embryonated chicken egg then followed by hemagglutinin agglutination test/hemagglutinin inhibition revealed that 7 samples were positively infected with AI virus. The amplification result of specific matrix gene primer was followed by electrophoresis on 2% agarose gel which were obtained in the form of a deoxyribonucleic acid (DNA) band at 276 bp for matrix gene and 1.725 bp for H5 gene for all isolates test. In conclusion, the virus which caused the death of various types of poultry in Aceh province is avian influenza A virus subtype H5.Key words: avian influenza virus, H5N1, serologic, matrix, heamaglutinin

RESPONS HSP-70 DAN KADAR KORTISOL AKIBAT PEMBERIAN KOMBINASI EKSTRAK JALOH DAN KROMIUM PADA AYAM BROILER YANG MENGALAMI CEKAMAN PANAS Sugito s; Erdiansyah Rahmi; Muhammad Isa
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui pengaruh pemberian kombinasi ekstrak jaloh dan kromium terhadap pembentukan heat shock protein-70 (HSP-70) dan kadar kortisol ayam yang mengalami cekaman panas. Dalam penelitian ini digunakan ayam broiler sebanyak 12 ekor yang dibagi atas empat perlakuan: Perlakuan I (EC), cekaman panas + dan kombinasi 1.000 mg ekstrak jaloh dengan 1.000 μg kromium per liter air minum, perlakuan II (E), cekaman panas + 1.000 mg ekstrak jaloh per liter air minum, perlakuan III (KD), cekaman panas + 0,0 mg ekstrak jaloh dan 0,0 μg kromium, dan Perlakuan IV (KL), tanpa cekaman panas dan 0,0 mg ekstrak jaloh dan 0,0 μg kromium. Masing-masing perlakuan terdiri atas tiga ulangan. Metode pemberian cekaman panas dilakukan dengan meningkatkan suhu dalam kandang pada 33±1° C selama lima jam per hari dalam waktu lima belas hari. Pemberian kombinasi ekstrak jaloh dan kromium dilakukan dengan cara melarutkannya dalam air minum dan diberikan selama dua jam (pemberian pukul 10.00) sebelum suhu di dalam kandang mencapai 33±1° C. Pengambilan sampel serum dan jaringan paru dilakukan pada hari ke-15 pelaksanaan penelitian (ayam umur 36 hari). Pengambilan sampel darah dilakukan sebelum ayam dipotong dan jaringan organ paru diambil setelah ayam dipotong. Pada sampel serum dilakukan pemeriksaan kortisol dengan enzymelinkedimmunosorbantassay (ELISA) dan deteksi HSP-70 di dalam jaringan paru dilakukan menggunakan metode imunohistokimia. Hasil penelitian ini menunjukkan bahwa pemberian cekaman panas pada suhu 33±1° C selama lima jam per hari dapat meningkatkan pembentukan HSP-70 dalam paru dan kadar kortisol dalam serum. Pemberian ekstrak jaloh secara tunggal lebih efektif menurunkan jumlah HSP-70 pada jaringan paru dibandingkan jika dikombinasi dengan kromium.

ISOLASI DAN IDENTIFIKASI VIRUS AVIAN INFLUENZA PADA BERBAGAI SPESIES UNGGAS SECARA SEROLOGIS DAN MOLEKULER (Isolation and Identification of Avian Influenza in Different Species of Poultry by Means of Serological and Molecular Methods) Teuku Zahrial Helmi; Charles Rangga Tabbu; Wayan Tunas Artama; Aris Haryanto; Muhammad Isa
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3378

The purpose of this research was to identify avian influenza (AI) virus using serological and molecular methods on poultry which suspected as AI infected in Aceh province. This study used 37 samples of tracheal and cloacal swabs and organs from various species of poultry that were collected from several districts/cities in Aceh. Samples were collected and put into transport media and stored at 4° C before sending to the laboratory. Samples were inoculated in specific pathogen-free of embryonated chicken egg with the age of 9-11 days for further serological and molecular examination. From 37 samples which infected to embryonated chicken egg then followed by hemagglutinin agglutination test/hemagglutinin inhibition revealed that 7 samples were positively infected with AI virus. The amplification result of specific matrix gene primer was followed by electrophoresis on 2% agarose gel which were obtained in the form of a deoxyribonucleic acid (DNA) band at 276 bp for matrix gene and 1.725 bp for H5 gene for all isolates test. In conclusion, the virus which caused the death of various types of poultry in Aceh province is avian influenza A virus subtype H5.Key words: avian influenza virus, H5N1, serologic, matrix, heamaglutinin

RESPONS HSP-70 DAN KADAR KORTISOL AKIBAT PEMBERIAN KOMBINASI EKSTRAK JALOH DAN KROMIUM PADA AYAM BROILER YANG MENGALAMI CEKAMAN PANAS Sugito s; Erdiansyah Rahmi; Muhammad Isa
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.341

Penelitian ini bertujuan mengetahui pengaruh pemberian kombinasi ekstrak jaloh dan kromium terhadap pembentukan heat shock protein-70 (HSP-70) dan kadar kortisol ayam yang mengalami cekaman panas. Dalam penelitian ini digunakan ayam broiler sebanyak 12 ekor yang dibagi atas empat perlakuan: Perlakuan I (EC), cekaman panas + dan kombinasi 1.000 mg ekstrak jaloh dengan 1.000 μg kromium per liter air minum, perlakuan II (E), cekaman panas + 1.000 mg ekstrak jaloh per liter air minum, perlakuan III (KD), cekaman panas + 0,0 mg ekstrak jaloh dan 0,0 μg kromium, dan Perlakuan IV (KL), tanpa cekaman panas dan 0,0 mg ekstrak jaloh dan 0,0 μg kromium. Masing-masing perlakuan terdiri atas tiga ulangan. Metode pemberian cekaman panas dilakukan dengan meningkatkan suhu dalam kandang pada 33±1° C selama lima jam per hari dalam waktu lima belas hari. Pemberian kombinasi ekstrak jaloh dan kromium dilakukan dengan cara melarutkannya dalam air minum dan diberikan selama dua jam (pemberian pukul 10.00) sebelum suhu di dalam kandang mencapai 33±1° C. Pengambilan sampel serum dan jaringan paru dilakukan pada hari ke-15 pelaksanaan penelitian (ayam umur 36 hari). Pengambilan sampel darah dilakukan sebelum ayam dipotong dan jaringan organ paru diambil setelah ayam dipotong. Pada sampel serum dilakukan pemeriksaan kortisol dengan enzymelinkedimmunosorbantassay (ELISA) dan deteksi HSP-70 di dalam jaringan paru dilakukan menggunakan metode imunohistokimia. Hasil penelitian ini menunjukkan bahwa pemberian cekaman panas pada suhu 33±1° C selama lima jam per hari dapat meningkatkan pembentukan HSP-70 dalam paru dan kadar kortisol dalam serum. Pemberian ekstrak jaloh secara tunggal lebih efektif menurunkan jumlah HSP-70 pada jaringan paru dibandingkan jika dikombinasi dengan kromium.

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