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E. Harlina

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Author-ID : 276355

Veterinary

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Immunohistochemical study of Ito cells of spontaneous cholangiohepatitis in broiler chickens Handharyani, E; Ochiai, K.; Winarsih, W.; Harlina, E.; Estuninngsih, S.
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 4 (2001)
Publisher : Indonesian Animal Sciences Society

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis and malformation of extrahepatic biliary tracts.Key words: Cholangiohepatitis, immunohistochemistry, ito cell. broiler chicken

Fasciolasis Pada Domba dan Kambing di Rumah Potong Hewan Kotamadya Bogor W. Winarsih; E. Estuningsih; A. Setiyono; E. Harlina
Media Veteriner Vol. 3 No. 1 (1996): Media Veteriner
Publisher : Media Veteriner

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Telah dilakukan penelitian fasciolasis pada domba dan kambing yang dipotong & Rurnah Potong Hewan Kotamadya Bogor pada bulan November sarnpai Desember 1992. Sebanyak 192 ekor domba dan kambing telah diamati terhadap kejadian fasciolasis pada organ hatinya. Metode penelitian yang dilakukan adalah dengan melakukan pemeriksaan berdasarkan skoring derajat kerusakan pada organ hati dengan melihat gambaran makroskoplk dan mikroskopik.Hasil pemeriksaan rnakroskopik diperoleh 14,06% menunjukkan gambaran hati normal dan 85.94% terinfeksi yaitu 76,04% terinfeksi akut dan 9.88% terinfeksi kronis.Sedangkan gambaran mikroskopik organ hati yang terinfeksi secara akut adalah perdarahan. degenerasi sel hati, peradangan dan proliferasi buluh empedu. infiltrasi selradang. dan admya 'globula leucocvte' pada mukosa buluh empedu. Pada infeksi kronis tampak fokus-fokus radang granuloma, mineralisasi dan fibrosis.4

Immunohistochemical study of Ito cells of spontaneous cholangiohepatitis in broiler chickens E Handharyani; K. Ochiai; W. Winarsih; E. Harlina; S. Estuninngsih
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis and malformation of extrahepatic biliary tracts.Key words: Cholangiohepatitis, immunohistochemistry, ito cell. broiler chicken

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