Claim Missing Document
Check
Articles

Found 12 Documents
Search

HISTOPATOLOGI HATI MENCIT PASCA PEMBERIAN SUSPENSI KEPEL (STELECHOCARPUS BURAHOL) SECARA INTRAGASTRIK SELAMA 14 HARI THE HISTOPATHOLOGY OF MICE LIVER TREATED BY KEPEL (STELECHOCARPUS BURAHOL) SUSPENSION INTRAGASTRICALLY FOR 14 DAYS Harlina, Eva; S Darusman, Huda; Alvernita, Gita
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 2, No 1 (2012): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.895 KB) | DOI: 10.33751/jf.v2i1.162

Abstract

This aim of this study was to examine the effect of Kepel (Stelechocarpus burahol) to the mice hepatocytes. Thirty male mice of 4 week aged were divided into three groups;control group was treated by aquadest, Dose 1x group was treated by 2.6 mg/g BW/daykepel powder (0.5 ml kepel suspension/day), and Dose 5x group was treated by 13 mg/gBW/day kepel powder (1.0 ml kepel suspension/day). The treatment was intragastricallyfor 14 days. The mice were euthanized and necropsy followed by the liver collection forhistopathology assay. The histopathological examination of liver showed hydropicdegeneration, cell death and extramedullary hematopoietic observed on mice hepatocytes.The ANOVA analysis showed that kepel caused increase significantly (p<0.05) ofhydropic degeneration and decrease significantly (p<0.05) of cell death of micehepatocytes.Key words: Stelechocarpus burahol, hydropic degeneration, cell death, extramedullary hematopoietic
GAMBARAN DARAH DAN KALSIUM DOMBA LOKAL (Ovis aries) YANG DIIMPLANTASI BIFASIK KALSIUM FOSFAT 70/30 SCAFFOLD 3D BERPOROS Rahmiati, Dwi Utari; Gunanti, Gunanti; Harlina, Eva; Dahlan, Kiagus
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.823 KB) | DOI: 10.19087/jveteriner.2018.19.4.547

Abstract

The aim of this research is to study the biocompatibility biphasic calcium phosphate with alginate combination by evaluating blood and calcium concentration. This study used three male sheeps, age 1.5 year old with an average weight of 20 kg. Anaesthesia is using an intravenous anaesthetic combination of 10% ketamine HCl (2 mg / kg BW) and 10% xylaxine HCl (0.1 mg / kg BW). The implant material is grown on the right sheep tibia. As a control, the left sheep tibia was made of defects but not implanted. The incision orientation is the straight line of the tibial femoral joint at medial, 3.6 cm from caudal to cranial and perpendicular from that point 5.7 cm to the distal direction. The defect is made using a bone drill to make a hole according to the size of the implant (diameter 0.8 cm and 0.4 cm long). Blood parameters (erythrocytes, hemoglobin (Hb), hematocrit (Hct), total leukocytes and differentiation of leukocytes; neutrophils, eusinophils, basophils, lymphocytes and monocytes) and calcium evaluations were performed on days 0, 7, 30, 60 and 90 post implantation. Based on complete blood count no significant of blood parameters. Based on the calcium value, there are significant values on the 30th and the 90th. Blood calcium concentration were decreased from day 0 to 90.
Infusum Daun Alpukat Sebagai Inhibitor Kristalisasi Kalsium Oksalat pada Ginjal (THE AVOCADO LEAVES INFUSUM AS INHIBITOR ON RENAL CALCIUM OXALATE CRYSTALIZATION) Madyastuti, Rini; Widodo, Setyo; Wientarsih, Ietje; Harlina, Eva
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.328 KB)

Abstract

Urine crystal is a crystal nucleus which tend to form urine stone. The case of urine stone seems to beincreased every year. Crystallization could induce acute tubular necrosis which impact on renal dysfunction.The signs of this condition are high level of urea, creatinine and decrease glomerulus filtration rate. Theobjective of this research was to evaluate the effects of infusum Persea americana Mill as an inhibitorcrystallization which induced by ethylene glycol on white male rats. 20 male rats were divided into 4groups; K1 as negative group received only distilled water ad libitum, K2 as positive group receiveddistilled water containing ethylene glycol, K3 (dose 5%) and K4 (dose 10%) as treatment groups receivedwater containing ethylene glycol and avocado leaves infusion. Phytochemsitry screening of infusion avocadoleaves consisted of flavonoid, saponin, tanine and quinone. Result of analysis showed that the level ofureum and creatinine on K2 was higher than K3 and K4 group. The increased level could be inhibited byinfusion avocado leaves. The measurement of glomerular filtration rate in treatment groups wassignificantly different (p<0.05). Descriptive histopathology observation showed that renal lesio in grouptreatment (K3 and K4) were declined. Large crystal calcium oxalate on K2 group was observed by usingpolarized microscope, whereas small crystal calcium oxalate were seen in the infusion of avocado leavesgroups. These result showed the ability of infusion of avocado leaves as an inhibitor on the growth ofcrystallization calcium oxalate
Gambaran kristal urin pada hewan model nefrolitiasis hasil induksi etilen glikol Purwono, Rini Madyastuti; Wientarsih, Ietje; Widodo, Setyo; Purwaningsih, Erni H; Harlina, Eva
ARSHI Veterinary Letters Vol. 3 No. 1 (2019): ARSHI Veterinary Letters - Februari 2019
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.24 KB) | DOI: 10.29244/avl.3.1.19-20

Abstract

Tingginya tingkat keterulangan kasus urolitiasis baik pada hewan maupun manusia, menuntut penelitian dalam mencari solusi menurukan angka keterulangan. Penelitian urolitiasis membutuhkan hewan model urolitiasis yang sesuai dengan kondisi sebenarnya. Salah satu metode yang digunakan adalah menciptakan kondisi hiperoksaluria. Penggunaan induksi etilen glikol menjadi metode yang paling umum digunakan. Induksi ini menghasilkan kristal kalsium oksalat monohidrat. 
HISTOPATOLOGI HATI MENCIT PASCA PEMBERIAN SUSPENSI KEPEL (Stelechocarpus burahol) SECARA INTRAGASTRIK SELAMA 14 HARI The Histopathology of Mice Liver Treated by Kepel (Stelechocarpus burahol) Suspension Intragastrically for 14 days Harlina, Eva; Sa’diah, Siti; S Darusman, Huda; Alvernita, Gita
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 2, No 1 (2012): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.895 KB)

Abstract

This aim of this study was to examine the effect of Kepel (Stelechocarpus burahol) to the mice hepatocytes. Thirty male mice of 4 week aged were divided into three groups; control group was treated by aquadest, Dose 1x group was treated by 2.6 mg/g BW/day kepel powder (0.5 ml kepel suspension/day), and Dose 5x group was treated by 13 mg/g BW/day kepel powder (1.0 ml kepel suspension/day). The treatment was intragastrically for 14 days. The mice were euthanized and necropsy followed by the liver collection for histopathology assay. The histopathological examination of liver showed hydropic degeneration, cell death and extramedullary hematopoietic observed on mice hepatocytes. The ANOVA analysis showed that kepel caused increase significantly (p<0.05) of hydropic degeneration and decrease significantly (p<0.05) of cell death of mice hepatocytes.Key words: Stelechocarpus burahol, hydropic degeneration, cell death, extramedullary hematopoietic 
Aktivitas Minyak Ikan dalam Menghambat Preneoplasia Kolon Mencit yang Diinduksi Azoksimetan dan Dextran Sodium Sulfate KUSMARDI, KUSMARDI; PRIOSOERYANTO, BAMBANG PONTJO; HARLINA, EVA; CORNAIN, SANTOSO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 2 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (632.063 KB)

Abstract

Studi epidemiologi penggunaan suplemen minyak ikan dan pengaruhnya terhadap kanker&nbsp; kolon masih kontroversial. Penelitian ini dilakukan untuk melihat pengaruh minyak ikan dalam&nbsp; menghambat preneoplasia kolon mencit yang diinduksi azoksimetan (AOM) dan dextran sodium sulfate&nbsp; (DSS). Digunakan mencit Balb/C yang diinduksi dengan AOM 10 mg/kg BB yang dilanjutkan dengan&nbsp; pemberian minum DSS 1% selama seminggu. Pemberian minyak ikan dilakukan per oral dengan dosis&nbsp; 1,5, 3, dan 6 mg per mencit per hari. Pemeriksaan histopatologi terhadap jaringan kolon yang diwarnai&nbsp; hematoksilin-eosin dilakukan dengan menghitung jumlah fokus radang, hiperplasia, jumlah sel mitosis,&nbsp; dan skor displasia pada 10 lapang pandang. Penurunan jumlah fokus radang terjadi sejak bulan ke-2&nbsp; dimana kelompok kontrol dan dosis rendah berbeda dengan dosis sedang dan tinggi (p&lt;0,05). Sedangkan&nbsp; pada bulan ketiga dan keempat, penurunan jumlah fokus radang terjadi sejak dosis rendah, sedang dan&nbsp; tinggi (p&lt;0,01). Jumlah sel epitel kripta kolon mitosis mengalami penurunan mulai bulan kedua hingga&nbsp; keempat. Jumlah fokus sel epitel mukosa hiperplasia juga mengalami perbedaan pada bulan kedua.&nbsp; Pemberian minyak ikan juga menghambat terjadinya displasia yang terjadi sejak bulan keempat pada&nbsp; semua kelompok perlakuan, dan pada bulan ketiga pada kontrol
The Role of Fennel Infusion on Estrous Cycle and Follicles Development of White Rats MAHESHWARI, HERA; SATYANINGTIJAS, ARYANI SISMIN; HARLINA, EVA; CAHYANINGSIH, UMI; EFFENDI, MULYATI; MUSTOFA, MUHAMMAD ADIB; BEKALANI, YENI KEZIA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 1 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.513 KB)

Abstract

This study aims to describe the estrous cycle and follicles development in female rats given infusion of fennel fruit that known to have phytoestrogen compound. Twenty five of female rats were used in this research and were divided into 5 groups. Group I was negative control group (NC), given 1 mL/100g bw of distilled water, group II was positive control group (PC), given 0.0045 mg/100g bw of ethinyl estradiol, groups III, IV and V were treatment groups (TI, T2 and T3), given fennel infusion at 36.5; 73 and 146 mg/100g bw respectively. All treatments were conducted every morning for 20 days by oral route. Changes of vaginal epithelium were observed through vaginal swabs previously stained with Giemsa stain. Histopathological examination of ovarian swere examined to reveal follicles development. Results showed that fennel fruit infusion extended the duration of estrous and metestrous phases, while shortened the proestrous and diestrous phases. Eventhough the longest estrous phase was found in T3 group, there was no significant effect on the lengthening of estrous cycle. Moreover, infusion of fennel fruit had no effect on the development of ovarian follicles, except tended to increase the number of corpus luteum.
Pathology of cutaneous blastomycosis in a cat Juniantito, Vetnizah; Harlina, Eva; Jumari, .; Handayani, Vici Eko; Ismawati, Iis
ARSHI Veterinary Letters Vol. 4 No. 1 (2020): ARSHI Veterinary Letters - Februari 2020
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.815 KB) | DOI: 10.29244/avl.4.1.3-4

Abstract

Cats are common as pet animals in Indonesia and live in close vicinity of human neighborhood, which make them potential for transmitting diseases to human. Blastomycosis is infectious disease caused by Blastomyces spp yeast. Here we describe morphopathology of blastomycosis found in skin of a Persian Cat. Grossly, the lesion were characterized by ulcerated wounds and multiple subcutaneous small nodules (2-5 mm in diameter) in the base of tail. Skin biopsy was made and further processed for histopathology. Microscopically, the lesion consist of epidermal necrosis, dermatitis, with coalescing dermal granulomatous inflammation, characterized by epitheloid and foreign type giant cells infiltrates. Numerous spherical-shaped structures and pseudohyphae which are Periodic Acid Schiff (PAS)-positive consistent with yeast morphology were found within the granulomas; morphology is also consistent with Blastomyces spp yeasts. Conclusively, multiple granulomatous dermatitis with evidence of Blastomyces yeasts is a hallmark of cutaneous blastomycosis. Exposure to Blastomyces spp yeast may possess infection threat to pet owners.
Aktivitas Ekstrak Etanol Daun Alpukat Terhadap Zat Nefrotoksik Ginjal Tikus (ACTIVITIES STUDY OF ETHANOL EXTRACTS OF AVOCADO LEAVES (PERSEA AMERICANA MILL) TO NEPHROTOXIC COMPOUND OF RAT’S KIDNEY) Wientarsih, Ietje; Harlina, Eva; Purwono, Rini Madyastuti; Utami, Ikrar Trisnaning Hardi
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Based on WHO’s data, about 80% peoples in the word use plant’s extract as herbal medicine. Avocadoleaves has used as herbal medicine which is works as diuretikum, reduce stone in renal, and cure sprue.The purpose of this research is to study activities of ethanol extract of avocado leaves to ethylene glycolinduction on rat’s kidney by histopatologically. This experiment was continuing Adha’s research (2009)with research design as follows: twenty male rats were divided into 4 groups, negative control group (KN),positive control group (KP), treated group I by ethanol extract of avocado leaves 100 mg/kg BW (E100), andtreated group 2 by 300 mg/kg BW (E300). Sampling kidney were fixed in BNF 10% for histopathologicalslide and stained by Hematoxylin-Eosin. The kidney histopathological changes were edema of glomerulus,and protein deposit in the lumen, hyalin droplet and necrotic tubules. The results showed that ethanolextract of avocado leaves can’t significantly to reduce glomerulus edema with percentage cases 31.9% and33.5% for E100 and E300 and tend not to reduce the occurrence of protein deposits in the lumen of thetubule. Ethanol extract of avocado leaves tend to reduce the incidence of tubular necrosis and it wassignificantly different from the KP group (p <0.05). It is caused by flavonoid in etanol extract of avocadoleaves works as diuretikum and antioxidant.
Efficacy differences of Queen’s crepe-myrtle (Lagerstroemia speciosa) and aloe vera extract on the interleukin-6 and osteoblast levels in the healing process of alveolar osteitis Bernadi, Willy; Hardianto, Andri; Yuza, Abel Tasman; Harlina, Eva
Padjadjaran Journal of Dentistry Vol 33, No 2 (2021): July 2021
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no2.21310

Abstract

Introduction: Alveolar osteitis is the most common complication after tooth extraction, which occurs 2 to 4 days after and causes disruption of wound healing. Pucuk Bungur/Queen’s crepe myrtle  (Lagerstroemia speciosa) and aloe vera have biological and pharmacological benefits in experimental animals: antimicrobial, anti-inflammatory, and antioxidant. They also affect the wound healing process.  These benefits are a good combination as an alternative remedy for alveolar osteitis treatment. This study was aimed to analyse the effect of Lagerstroemia speciosa compared to aloe vera extracts gel on the wound healing process after tooth extraction in Sprague-Dawley mice with alveolar osteitis. Methods:  This research was an experimental laboratory. Twenty-seven Sprague-Dawley mice were randomly divided into three groups. The first group was the osteitis (control) group treated with 1:1000 adrenaline insertion for 1 minute on the left maxillary first molars socket; the second group was the osteitis group administered with the application of Lagerstroemia speciosa, and the third group was the osteitis group administered with the application of aloe vera gel. Normal mice in the control group were not given any treatment, only osteitis mice. The interleukin-6 level was examined after, and the number of osteoblasts was also calculated on the 3rd, 5th and 14th day after the necropsy was performed. Data were analysed with one-way ANOVA to compare the effectiveness of wound healing of alveolar osteitis in each group. Results: From the data analysis, the osteitis group applied with  Lagerstroemia speciosa gel had good activity in the inflammation phase of the healing process of alveolar osteitis compared to other groups. Conclusion: Lagerstroemia speciosa can be a potential alternative treatment to reduce inflammation and accelerate the healing of osteitis because it shortens the inflammatory phase and accelerates collagen production in wound healing.