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Penurunan Glukosa dan Perubahan Profil Lipida Serum Tikus Sprague- Dawley Hiperglikemia-Hiperkolesterolemia Akibat Asupan Sorbitol-Oleat Poliester (SOPE) Murdiati, Agnes; Wuryastuty, Hastari; Marsono, Y.; Harmayani, Eni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.509 KB) | DOI: 10.24002/biota.v14i3.2576

Abstract

Disorder of carbohydrate metabolism in the Diabetes Mellitus patients resulted in increasing of cholesterol synthesis. This condition increases the risk of heart attack or coronary heart disease, therefore it is recommended to lower fat intake. One of the alternatif to lower fat intake is by replacing fat with fat replacer in the diet. The aim of the present study is to evaluate the effect of replacing 75% of fat with SOPE on the glucose and lipid profile in diabetic-hipercholesterolemic rats. Eihgteen Sprague Dawley rats were devided into 2 groups of 9 rats. They were fed with hipercholesterol diet and were diabetic induced with alloxan IM injection. One group was fed with 75% SOPE substitute the fat in the standard diet and the other group was fed with standard diet. The result indicates that the oil substitution of standard diet of Sprague Dawley rats for sorbitol-oleic polyester as much as 75% gives an advantage to rats which suffer hiperglikemic-hypercholesterolemic because it decreased the glucose, total cholesterol, LDL cholesterol, and LDL/HDL cholesterol ratio, and did not decrease the HDL cholesterol, but increased triglyceride serum.
Effect of Probiotic Lactobacillus sp. Dad13 on Humoral Immune Response of Balb/C Mice Infected with Salmonella typhimurium Kusumawati, Ika Dyah; Harmayani, Eni; Asmara, Widya
Indonesian Journal of Biotechnology Vol 11, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.484 KB)

Abstract

An indigenous strain of lactic acid bacterium (LAB) identified as Lactobacillus spp. Dad13 (Dad13), isolatedfrom traditional fermented buffalo milk, was found to be potential as probiotic. The aim of this research was to studythe effect of probiotic Dad13 on humoral immune response of Balb/C mice infected with Salmonella typhimurium. Thespecific objective was to find out the effect of different Dad13 consumption time (before and along with infection of S.typhimurium) on the humoral immune response of Balb/C mice. The experiment was conducted by in vivo trial on 20males of Balb/C mice, age of 6-8 weeks, fed with AIN-93 standard diet. The mice were assigned into 4 groups. Eachgroup received the following treatments, ie. Dad13 only, Dad13 before infection, Dad13 along with infection andSalmonella infection only. A volume of 100 μl Dad13 cell suspensions (1010 CFU/ml) were given by oral forced feedingdaily for a week, at week 3 for group before infection and at week 4 for group of Dad13 only and Dad13 along withinfection. Salmonella infection (109 CFU/ml) was given once orally at week 4 to all groups except group treated withDad13 only. The humoral immune response of Balb/C mice was detected 2 weeks after infection by measuring thetiters of IgG and IgA specific from serum and mucosal intestinal liquid samples using Enzyme-linked ImmunosorbentAssay (ELISA) method. The result indicated that humoral immune response of Balb/C mice consuming Dad13 beforeand along with Salmonella infection were significantly different (p<0.05). Dad13 consumption along with Salmonellainfection increased circulated IgG and IgA as well as secretory IgA. It can be concluded that Dad13 probiotic feedingalong with infection increased humoral immune response more significantly compared to that before infection.Key words : Probiotic, Lactobacillus sp. Dad13, Immune response, Salmonella typhimurium
Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015 Harmayani, Eni; N, Nofisulastri; Bachruddin, Zaenal
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.738 KB)

Abstract

objectives were to study the growth pattern of Pediococcus sp. NWD 015 and bacteriocin activity, extractionand characterization of bacteriocin, and to determine the effect of storage time and temperature on bacteriocinactivity. Results showed that the bacteriocin activity increased during growth and reached the highest activity duringstationary phase. The maximum bacteriocin production reached after incubation of the cell for 12 h at 37oC in TGEbroth and decreased after 96 h incubation. Extraction with adsorbtion-desorbtion method could increased a specificactivity of bacteriocin. Bacteriocin from Pediococcus sp. NWD 015 is inactivated by Proteinase-K; however it is stillactive by heat treatment at 121oC for 15 min and over pH 2 – 11. Bacteriocin of Pediococcus sp. NWD 015 was effectiveagaints Enterococcus faecalis, Staphylococcus aureus, Eschericia coli, Listeria monocytogenes but not against Salmonellathypimurium. The molecular weight of bacteriocin is 4.95 kDa.Keywords : Bacteriocins, Pediococcus sp NWD 015.
KARAKTERISASI GEN PENYANDI PEDIOSIN PAF-11 PADA Pediococcus acidilactici F-11 [Characterization of the Pediocin PaF-11 Encoding Gene in Pediococcus acidilactici F-11] Marwati, Tri; Richana, Nur; Harmayani, Eni; Rahayu, Endang S
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.137 KB) | DOI: 10.14203/beritabiologi.v11i2.495

Abstract

Pediocin PaF-11 is a ribosomally synthesized antimicrobial peptide produced by Pediococcus acidilactici F-11. The objectives of this research is to find out the location and the nucleotide sequence of gene, which is involved in the production of pediocin PaF-11. Results showed that the pediocin PaF-11 from the cured cell of P. acidilactici F-11 loss the activity, suggested that the pediocin PaF-11 gene was carried in the plasmid. Agarose gel electrophoresis of P. acidilactici F-11 plasmid DNA with marker λDNA/HindIII showed that pediocin PaF-11 gene was carried in 12 kb plasmid. Amplification pediocin PaF-11 gene from P. acidilactici F-11 showed that uncured P.acidilactici F-11 culture contain plasmid DNA, indicated by amplification of the papA gene (256 bp). Cured P. acidilactici F-11 culture, plasmid eliminated, indicted by no aplicon DNA detected. This result also suggested that pediocin PaF-11 gene in P. acidilactici F-11 was carried in plasmid. Nucleotide of pediocin PaF-11 encoding gene was sequenced The alignment of that nucleotide sequence showed that pediocin PaF-11 encoding gene have the same sequence with pediocin PA.1 encoding gene in P. acidilactici PAC1.0 and P. acidilactici K10 and pediocin AcH encoding gene in P. acidilactici LB 42-923 and P .parvulus ATO77, and pediocin CP2 in P. acidilactici MTCC 5101.
PURIFICATION AND CHARACTERIZATION OF THE NEWLY THERMOSTABLE PROTEASE PRODUCED BY Brevibacillus thermoruber LII ISOLATED FROM PADANG CERMIN HOTSPRING, INDONESIA Zilda, Dewi Zeswita; Harmayani, Eni; Widada, Jaka; Asmara, Widya; Irianto, Hari Eko; Patantis, Gintung; Fawzya, Yusro Nuri
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 1 (2014): May 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i1.91

Abstract

Thermo stability is among of the vital enzyme characteristics for industrial application. Brevibacillus thermoruber LII was obtained as a potential isolate from the previous researchwhich screened the potential thermostable protease producing bacteria from Indonesian hotspring.The newly thermostable protease produced by thermophilic Brevibacillus thermoruber LII hadbeen purified and characterized. It was predicted that the pure enzyme obtained from Brevibacillusthermoruber LII was homo hexameric, having molecular weight of 36 kDa unit protein and itsnative was 215 kDa. In addition, it was also a neutral metalo serine protease according tobiochemical tests that it was totaly inhibited by PMSF (Phenylmethanesulfonyl fluoride) and EDTA(Ethylenediaminetetraacetic acid). It showed optimum activity at pH of 8 and active in acidic buffer(up to pH of 4). All of metal ion in the form of chloride salt (2.5 mM) which were tested on theenzyme enhanced the enzyme activity but Li2+. Ca2+ion increased the activity and the stability ofenzyme against thermal. The enzyme also showed the stability against solvent. The protease LIIhad optimum temperature at 60oC without CaCl 2and 80 – 85oC with addition of 2.5 mM CaCl 2. TheK Mand V maxvalues for the purified protease LII were 27.2 mg/ml or 0.362 – 0.272 M for substrateHammersteinCasein (MM 75–100 kDa) and 261.1 µg/minute/ml, respectively.
Peningkatan aktivitas fagositosis dan produksi nitrit oksida pada makrofag peritoneum tikus Sprague Dawley yang diberi Lactobacillus plantarum Mut7 dan ekstrak serat ubi jalar Lestari, Lily Arsanti; Soesatyo, Marsetyawan Heparis Nur Ekandaru; Iravati, Susi; Harmayani, Eni
Jurnal Gizi Klinik Indonesia Vol 9, No 2 (2012): Oktober
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, FK-KMK UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.572 KB) | DOI: 10.22146/ijcn.15381

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Background: Macrophages play an important role as part of the innate immune response in the gut and they represent one of the first lines of nonspecific defense against bacterial invasion. Previous studies indicated that probiotics and prebiotics may act as an immunomodulator agents. Nevertheless, research on the immunomodulatory effect of local materials has never been performed.Objective: To study the effects of Lactobacillus plantarum Mut7 and sweet potato fiber on the activity and Nitric Oxide (NO) production of peritoneal macrophages of Sprague Dawley rats.Method: Ninety six Sprague Dawley rats aged 8 weeks were divided into two groups; A (not infected with Salmonella typhimurium) and B (infected with S. typhimurium). Each group was divided into 4 subgroups and assigned to standard AIN-93M diet (KON), 109 CFU/ml of L. plantarum Mut7 (PRO), modified AIN-93M diet with sweet potato fiber (PRE), and both component (SIN). After 3 weeks of treatment, 6 rats of each subgroup were sacrificed and the peritoneal macrophages were isolated and analysed for its activity and NO production. The rest of the rats continued the treatments for another 2 weeks. At the end of the experiment, they were sacrificed and the peritoneal macrophage were isolated and analysed for its activity and NO production.Results: Oral administration of  L. plantarum Mut7, sweet potato fiber, or both improve phagocytic activity of peritoneal macrophage which was indicated by an increase in the percentage of macrophages that phagocyte latex particles (p<0.05) and an increase in the number of latex particles engulfed by macrophages either after 3 or 5 weeks of treatment (p<0.05). Oral administration of L. plantarum Mut7, sweet potato fiber, or both were unable to increase the nitric oxide production after 3 weeks of treatment (p>0.05), but after 5 weeks of treatment the production of NO was significantly increased (p<0.05).Conclusion: L. plantarum Mut7, sweet potato fiber, or both increase the non specific immune response as they could improve the activity and NO production of peritoneal macrophages.
PERUBAHAN KARAKTERISTIK FISIKOKIMIA, HIDROFOBISITAS DAN KRISTALINITAS PADA PATI JAGUNG VARIETAS BISI-18 TERASETILASI Rosiana, Nita Maria; Harmayani, Eni; Pranoto, Yudi
Jurnal Teknologi Pertanian Vol 22, No 2 (2021)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtp.2021.022.02.3

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ABSTRAK Jagung merupakan salah satu komoditas pertanian yang berpotensi besar untuk dikembangkan secara komersial. Cookies yang dibuat dari 100% tepung jagung mengakibatkan penerimaan konsumen yang menurun karena tekstur cookies yang keras. Kekerasan pada cookies dari jagung dapat pula disebabkan oleh kemampuan pati dalam mengikat air dan lemak yang rendah serta struktur pati yang kompak. Salah satu metode modifikasi pati yang digunakan untuk memperbaiki sifat pati adalah asetilasi. Persyaratan derajat asetilasi pada pati yang dapat digunakan untuk menjadi produk olahan adalah 0,01-0,2. Tujuan dari penelitian ini adalah mendapatkan rekayasa proses asetilasi untuk mendapatkan derajat substitusi 0,2 dan mengetahui perubahan karakteristik fisikokimia, hidrofobisitas dan kristalinitas pada pati dengan derajat substitusi 0,2. Jagung varietas Bisi-18 yang digunakan untuk penelitian berasal dari Kabupaten Bantul, Yogyakarta. Tahapan penelitian meliputi ekstraksi pati jagung dan modifikasi asetilasi dengan Rancangan Acak Kelompok. Faktor 1 adalah konsentrasi asetat anhidrat (2,4,6%) dan faktor 2 adalah waktu reaksi (5,10,15 menit). Hasil penelitian menunjukkan bahwa proses asetilasi terjadi pada pH 8-8,4; suhu reaksi 28-30oC selama 15 menit dengan konsentrasi asetat anhidrat 4% (v/w) menghasilkan pati terasetilasi dengan derajat subtitusi 0,2. Pati jagung tersebut mengalami perubahan karakteristik yaitu lebih hidrofob dan kristalinitas menurun. Pati terasetilasi dengan derajat substitusi 0,2 berpotensi untuk dijadikan bahan baku kue kering seperti cookies.ABSTRACT Corn is one of the agricultural commodities with great potential to be developed commercially. Cookies from 100% corn flour resulted in reduced consumer acceptance, caused by hard cookies. The hardness of the cookies from corn can also be caused by the low ability of starch to binding water and fat, and the compact structure of starch. One of the starch modification methods used to improve starch properties is acetylation. The degree of acetylation in starch that can be used to be a processed product is 0.01-0.2. The aims of this study were obtaining an acetylation process to obtain a degree of substitution of 0.2 and to determine the changes in the characteristics of physicochemical, hydrophobicity, and crystallinity of starch with a degree of substitution of 0.2. Corn variety Bisi-18 used for research comes from Bantul Regency, Yogyakarta. The research stages included extraction of corn starch and modification of acetylation with a randomized block design. The first factor was the concentration of acetate anhydrous (2,4,6%) and the second factor was the reaction time (5,10,15 minutes). The results showed that the acetylation process occurred at pH 8-8.4; reaction temperature is 28-30oC for 15 minutes with  4% (v/w) acetate anhydrous to produce degree of substitution 0,2 acetylated corn starch. The characteristic of degree of substitution 0,2 acetylated corn starch is more hydrophobic and decreased crystallinity compared to natural starch. Acetylated starch with degree of substitution 0,2 has potential to be used as raw material for pastries such as cookies.
Screening of Immunostimulatory Activity from Indonesian Kampung (Gallus domesticus) Egg White Water Extract: in vitro study Mulyani, Rizka; Harmayani, Eni; Nurliyani; Ishida, Momoko; Nishi, Kosuke; Sugahara, Takuya
Journal Akademik Universitas Swiss German Vol. 3 No. 1 (Aug 2021)
Publisher : Swiss German University & Perhimpunan Penggiat Pangan Fungsional dan Nutrasetikal Indonesia (P3FNI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33555/jffn.v3i1.68

Abstract

The most popular Indonesian native chicken, known as kampung chicken, is maintained under free-range conditions therefore it is prone to high environmental stress. Indonesian native chicken and its product are always regarded as having health benefits compared to commercial chicken by Indonesian society. But there is still limited report regarding Indonesian native chicken and its product. This study focused on screening immunostimulatory activity from Indonesian native chicken egg white using in vitro approaches as a functional food. Indonesian native chicken egg white (NEW) was extracted using distilled water supplemented to human-human hybridoma HB4C5 cells to examine the IgM production stimulating activity using ELISA. The gene expression was also examined using qRT-PCR. The ability of NEW on stimulating immunoglobulin production by mouse splenocytes was analyzed. Commercial egg white water extract (CEW) was used as a comparison. The data were analyzed using One-way ANOVA and continued by using post-hoc analysis using Tukey’s multiple comparison test. The results showed that NEW and CEW modulated IgM production by the HB4C5 cells 8.72-fold and 6.75-fold, respectively, compared to control. NEW stimulated immunoglobulin (Ig) production by the mouse splenocytes higher than CEW. To conclude, NEW provides an immunostimulating activity that can potentially act as a health-promoting food.
Pengaruh pemberian jelly mengandung glukomanan porang (Amorphophalus oncophyllus) dan inulin sebagai makanan selingan terhadap berat badan, IMT, lemak tubuh, kadar kolesterol total, dan trigliserida pada orang dewasa obesitas Saputri, Rissa; A’yun, Rizka Qurrota; Huriyati, Emy; Lestari, Lily Arsanti; Rahayoe, Sri; Yusmiati, Yusmiati; Sulistyo, Okta Haksaica; Harmayani, Eni
Jurnal Gizi Klinik Indonesia Vol 17, No 4 (2021): April
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, FK-KMK UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.58343

Abstract

Effect of jelly containing of porang (Amorphophalus oncophyllus) glucomannan and inulin as a snack to the body weight, BMI, body fat, total cholesterol, and triglyceride levels in obese adultBackground: Obesity is a major risk factor for noncommunicable diseases, especially cardiovascular disease. Porang glucomannan and inulin are water-soluble fibers that have been widely studied for their health benefits. Jelly products that contain both types of fiber are expected to have the ability to support the diet program in adults with obese nutritional status. Objective: To determine the effect of giving jelly containing a combination of glucomannan porang and inulin on body weight, BMI, body fat, total cholesterol, and triglyceride levels in obese adults.Methods: This research is a randomized controlled trial using a pre and post-test design. A total of 55 subjects who live in the Special Region of Yogyakarta, participated in the study until the end of 8 weeks. Subjects were divided into 3 groups; 18 people in the group who were given jelly containing glucomannan porang and inulin (porang-inulin jelly group), 17 people in the group who were given placebo jelly (placebo jelly group), and 20 people in the negative control group who did not get jelly (negative control group). All subjects received a low-calorie diet intervention of 1,500 kcal in the first month and 1,200 kcal in the second month. Results: There was a significant reduction in body weight and BMI (p<0.05) in all groups, but there was no difference in the effect between the porang–inulin jelly group and the other groups (p>0.05). Clinically, there was a significant decrease in body fat percentage in the porang-inulin jelly group, in subjects with the category of an excess fat percent (-0.90 ± 0.49%) and obesity (-1.48 ± 0.83%). A significant decrease in fat percentage also occurred in the porang-inulin jelly group, both in male subjects (-1.42 ± 0.50%) and female (-1.15 ± 0.82%), while in the placebo jelly group and negative control, the decrease did not occur in all categories and genders. There was an increase in total cholesterol levels in the negative control group (p<0.05) after the study (+7.45 ± 13.18 mg/dL). In the porang-inulin jelly group, the increase in cholesterol levels did not occur significantly either in general or based on clinical categories and gender. Conclusion: Consumption of porang-inulin jelly may support weight loss programs, BMI, percent body fat, and inhibit the increase of total cholesterol and triglyceride levels in adults with obese nutritional status.
KARAKTERISTIK AGROEKOLOGI DAN POTENSI TANAMAN GARUT PADA BERBAGAI VARIASI KETINGGIAN DI KABUPATEN GARUT, JAWA BARAT Rohandi, Asep; Budiadi; Hardiwinoto, Suryo; Harmayani, Eni
Jurnal Wana Tropika Vol 6 No 1 (2016)
Publisher : Fakultas Kehutanan Institut Pertanian STIPER Yogyakarta

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Abstract

Arrowroot (Maranta arundinacea L.) is a multipurpose plant that can grow under the stands so that the species has the potential to develop in the pattern of agroforestry. Initial information of arrowroot plant population as a source of germplasm for plant material/superior varieties and their agroecological characteristics in the field is indispensable. The study aims to determine the distribution, agroecological characteristics and potency of arrowroot in Garut regency. The methods include: (1) the study of literature and direct communication with stakeholders and the public, (2) field survey to collect data on distribution, cultivation status, abundance and productivity of arrowroot plant using a single plot, and (3) data collection of arrowroot agroecological characteristics include: vegetation analysis and geoklimat factors. The research results indicate that the arrowroot populations distribute in groups with a fairly broad ecological range i.e. altitude 6-1351 masl, temperature 20-36o C, relative humidity RH 40-72%, light intensity 12-56% dan pH 4.16-7,40 (acid to neutral) and in the low to high soil fertility condition. Falcataria moluccana as the major private forest species dominates in all zones of altitude, especially on the stage of the tree. Arrowroot abundance by elevation zones is 63,750 plants/ha (lowlands), 43,864 plants/ha (plains) and 73.333 plants/ha (plateau). The potential production for all elevations ranged from 15.40 to 163.2 g/plants. Cikajang and Cilawu populations have good potential to produce the high yield of tuber for arrowroot cultivation in Garut District. Keywords : Maranta arundinacea, Agroecological characteristics, Elevation, Potency, Garut District