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SUPLEMENTASI BAGASE DENGAN ENZIM SELULASE DAN PENGARUHNYA TERHADAP KECERNAAN BAHAN KERING, BAHAN ORGANIK DAN SERAT KASAR SECARA IN VITRO Marlida, Yetti; Zain, Mardiati
Jurnal Riset Kimia Vol 1, No 1 (2007): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v1i1.98

Abstract

 ABSTRACTSupplementation of cellulase enzyme to agro-industrial residues can increase the nutritional value of the product. Advances in industrial biotechnology offer potential opportunities for economic utilization of agro-industrial residues such as palm pres fiber, rice straw, corn straw and bagase for animal feed. Bagase is a complex material, is the major by product of the sugar cane industry.  This experiment examined the effect of applying a cellulase enzyme (Siqma & Co) on the digestibility of bagase in vitro.  The enzyme was applied in liquid form at  concentrations 1.0; 1.5 and 2.0 (g/100 g DM) to bagase. The bagase was incubated with enzyme for 48 h before in vitro digestion. The digestibility of dry matter, organic matter and fiber  in vitro was detected. The results showed that digestibility of dry matter, organic matter and fiber were significantly different (P<0.01) across treatments. The increased of the cellulase enzyme supplemented to bagase can increased the nutritive values of the product and directly digestibility of dry matter, organic matter and fiber higher.  Results from this study indicated that direct application of enzymes to bagase was capable to improving  digestibility in-vitro. Key words :  cellulase enzyme, bagase, digestibility, in vitro
PRODUKSI GLUKOSA DARI BATANG KELAPA SAWIT MELALUI PROSES HIDROLISIS SECARA ENZIMATIS MENGGUNAKAN AMILASE TERMOSTABIL Marlida, Yetti; -, Mirzah; Arief, Syukri; Amru, Khoirul
Jurnal Riset Kimia Vol 7, No 2 (2014): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v7i2.190

Abstract

ABSTRACTThis study aimed to explore the optimization of the concentration of oil palm trunks andthermostable amylase enzyme preformance produce highest sugar (glucose total and reducingsugars) as well as the degree of polymerization. The design used in this study was a completelyrandomized design (CRD) factorial using 2 factors: factor A which consists of four levels ofamylase enzyme that A1: 250 units / kg, A2: 500 units / kg, A3: 750 units / kg and A4: 1000 units/ kg. Factor B is the concentration of oil palm trunk yitu B1: 20% (200 g / 1 liter) B2: 40% (400g / 1 liter) and B3: 60% (600 g / 1 liter) were repeated 3 times. The results showed that there is ahighly significant interaction (P <0.01) in the levels of amylase enzyme (factor A) with theconcentration of oil palm trunks (factor B) on reducing sugar, siqnificantly interaction (P <0.05)on total sugars and degree of polymerization (DP) . This research can be concluded that the besttreatment at a concentration of 60% oil palm trunks and levels 250 unit/kg of an enzymeconcentration that produces of total sugars 7.86 mg/ml, reducing sugar 15.69 mg /ml and degreeof polymerization 0.50Keywords : amilase, total gula, gula pereduksi, derajat polimerisasi.
Toxicity Test Pediocin N6 Powder Produced from Isolates Pediococcus Pentosaceus Strain N6 on White Mice Ketaren, Nurjama'yah Br.; Marlida, Yetti; Arnim, Arnim; Yuherman, Yuherman; Rusmarilin, Herla
Journal of Food and Pharmaceutical Sciences Vol 4, No 1 (2016): J. Food Pharm. Sci (January-April)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.941 KB) | DOI: 10.14499/jfps

Abstract

Pediocin N6 powder is a bacteriocin the heat resistant derived from isolates Pediococcus pentosaceus strain N6. These were isolated from water source heat Rimbo Panti West Sumatra. Pediocin N6 powder has high antimicrobial activity, so the potential to be used as biopreservatif on meat and food processing industry which involves heating. Toxicity test was conducted to determine the effects of the toxic effect of a single dose of oral Pediocin N6 powder in test animals male white mice to determine the LD50 and see changes in body weight of mice for 15 days of treatment. Changes in body weight of mice was determined by using non factorial experiment in a completely randomized design consists of 4 treatments and 5 replications. The test animals were divided into 4 groups and each treatment consisted of 5 mice. The treatments tested consisted of Pediocin N6 powder 5000 mg/kg body weight, 10,000 mg/kg body weight, 15,000 mg /kg and 20,000 mg /kg body weight of mice. The test results showed that the Pediocin N6 powder up to a dose of 20 000 mg/kg in a single oral dose administration, there are no death of mice to 15 days of treatment. Based on the LD50 value of a single oral dose can not be calculated, based on it can be stated LD50 value pseudo Pediocin N6 powder greater than 20,000 mg /kg in male mice. The average changes in body weight of mice at a dose of Pediocin N6 powder treatment of up to 20,000 mg/kg every 2 days weighing from day 1 to day 15 of 2.1 gr. Based on these tests Pediocin N6 powder safe used as industry biopreservatif on meat and food processing involves heating.Key word: Toxicity Test, Pediocin N6 Powder, White Mice, Biopreservatif on Meat
IPTEK BAGI MASYARAKAT PADA KELOMPOK TANI TERNAK DI SUNGAI PERMAI, LAMBUNG BUKIK Evitayani Evitayani; Yetti Marlida; Ahadiyah Yuniza; James Hellyward; , Suyitman , Suyitman; Harnentis Harnentis
Jurnal Hilirisasi IPTEKS Vol 1 No 3.a (2018)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (713.934 KB)

Abstract

West Sumatra Province has relatively extensive agriculture with feed ingredients such as rice straw, Rumput Gajah and legume groups that can be used for beef cattle needs. The total area of Padang City is 694.96 km², and more than 60% of this area, about ± 434.63 km² is a hilly area covered by protected forests, while the rest is an effective urban area. While the topography of the city varies, 49.48% of the land area of Padang City is in a slope of more than 40% and 23.57% is in the slope of the slope. The eastern region consists of several sub-districts, starting from north to south, respectively from Koto tangah, Pauh, Kuranji, and Lubuk sub-districts. The Pauh region, such as the village of Lambung Bukik, belongs to the Pertides forum program (universities for villages) which collaborates with the 2019 Chancellor's MOU with the Ministry of Education PDTT) to prepare and implement programs to help build villages. Some of their income is obtained from raising livestock and farming. In general, both agricultural and livestock businesses are still carried out traditionally, so it is not surprising that the results obtained are relatively low. Cows are only ground or tethered at night, while in the afternoon they are released to look for food on the grass or in the sleeping area around the village. Only a small amount of effort has been made to raise livestock intensively by supplying and providing sufficient and regular food. The low level of livestock production is caused by the lack of knowledge of farmers in the proper way of raising livestock, also because of the lack of food both forage and the high price of concentrate. With the increase in the livestock population, it certainly requires more and sufficient forage throughout the year. However, the provision of forage has experienced serious obstacles. One of them is the existence of a dry season that causes a decrease in forage production. Therefore, the business of developing beef cattle will be more profitable if you can find alternative substitutes for conventional forage with the use of silos as feed fermentation sites. The use of agricultural products (such as rice straw) and other food crops as animal feed is expected to address the above problems. This is possible because cattle breeding is generally integrated with other farming businesses, especially food crops (rice fields) so that the results of agricultural follow-up will be available throughout the year. Therefore, there is a need for a kind of touch of feed technology with the use of agro-industry by products such as straw which are proven to be available at all times. The implementation of the application of rice straw ammoniation technology in the field with the direct administration of ammonia which has been removed and chicken manure is given. Participation and motivation of farmer groups in participating in a series of service activities is very high. Because all this time there has never been any guidance related to the technical and management aspects in the maintenance of beef cattle. Farmers already know how to manage fattening beef cattle such as giving concentrates such as coconut cake, soybean meal, fish meal, tofu pulp and bran and giving premix minerals.
The Activity Of Cellulose Enzyme From Indigenous Bacteria "Bacillus Sp YLB1" As Bioactivator Yunilas .; Lili Warly; Yetti Marlida; Irsan Riyanto
Jurnal Peternakan Integratif Vol 7, No 2 (2019)
Publisher : Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.391 KB)

Abstract

This study aims to test the activity of cellulase enzyme (CMCase) from indigenous bacteria "Bacillus Sp YLB1" as bioactivator of palm oil based feed. The treatment consists of various combinations of substart (feed), namely: P1 = 80% palm leaves + 10% palm kernel cake + 10% sludge; P2 = 60% palm leaves + 20% palm kernel cake + 20% sludge and P3 = 60% palm leaves + 30% palm kernel cake + 30% sludge. The parameters observed were cellulase enzyme activity (CMCase) on various combinations of palm oil waste and fermentation time. The results showed that cellulase enzyme (CMCase) production of Bacillus sp YLB1 bacteria during fermentation fluctuated ie 0.143 Units / ml; 0.372 Units / ml; 0.588 Units / ml; 1.013 Units / ml; 0.906 Units / ml; 1,065 Units / ml and 1,198 Units / ml.The activity of cellulase enzyme (CMCase) on substrate P1 (0.460 Unit / ml) is lower than P2 (0.897 Unit / ml) and P3 (0.908 Units / ml). From the results of this study can be concluded that cellulase enzyme activity indigenous bacteria Bacillus sp YLB1 influenced by the combination of substrate and fermentation time. The P3 substrate fermented with Bacillus sp YLB1 for 7 days gave a more optimal result than the other treatments.   Keywords: Cellulase enzyme, indigenous bacteria, substart (feed)
PENERAPAN PRODUKSI SAPI POTONG DAN PAKAN TERNAK RUMINANSIA DI UNIVERSITAS ANDALAS Mardiati Zain; Yetti Marlida; Rusmana setianingrat; Erpomen Erpomen; Rahmiwati Rahmiwati
Jurnal Pengabdian Warta Andalas Vol 24 No 4 (2017): Warta Pengabdian Andalas
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sapi potong merupakan komoditas peternakan yang potensial untuk dikembangkan guna memenuhi kebutuhan daging untuk masyarakat Program swasembada daging sapi dan kerbau yang dicanangkan oleh pemerintah menuntut kita sebagai praktisi dibidang peternakan untuk berusaha meningkat preoduktivitas sapi potong tersebut. Tingkat produksi sapi potong di Indonesia masih rendah yang terlihat dari tingginya impor sapi dari tahun ke tahun. Untuk tahun 2008 impor sapi mencapai 516.992 ekor. Rendahnya produktivitas ini dapat disebabkan karena permasalahan nutrisi ternak yaitu mutu nutrisi pakan yang rendah, baik ditinjau dari kadar nutriennya maupun kecernaannya, serta ketersediaan bahan pakan yang fluktuatif sehingga tidak menjamin kesinambungan produksi ternak. Untuk mengatasi masalah tersebut dirasakan perlu melakukan terobosan dengan cara memproduksi pakan ternak ruminansia skala industri seperti pakan non ruminansia (unggas) yang sudah lebih dulu berkembang. Untuk menunjang program pemerintah tersebut maka kami dari Fakultas Peternakan Universitas Andalas mencoba untuk mengembangkan usaha penggemukan sapi potong serta memproduksi pakan ternak potong yang berkualitas dan memenuhi standar kebutuhan ternak melalui program IbIKK. Program ini akan dilakukan selama 2 tahun dimana pada tahun pertama telah diproduksi sapi potong yang siap dilempar kepasaran dengan pemberian makanan yang diproduksi sendiri. Sampai saat ini sudah di pelihara 15 ekor sapi potong yang sudah akan dipasarkan untuk Qurban sebanyak 10 ekor. Sapi diberi makan dengan ransum yang diproduksi sendiri berbasis limbah sawit. Target dari kegiatan ini adalah mampu meningkatkan produktivitas ternak potong dan memproduksi pakan ruminansia yang berkualitas dengan harga terjangkau sehingga program ketahanan pangan bisa terwujud.
PRODUKSI ENZIM SELULASE TERMOSTABIL DARI BAKTERI NG2 MENGGUNAKAN BERBAGAI SUMBER SELULOSA ASAL LIMBAH PERTANIAN DAN PERKEBUNAN (Production of Thermostable Cellulase Enzyme by NG2 Bacteria Using Various Cellulose Sources from the Agriculture Waste) Rahmad Fani Ramadhan; M. Montesqrit; Yetti Marlida
Jurnal Ilmu dan Teknologi Peternakan Vol. 8 No. 2 (2020)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jitp.v8i2.8171

Abstract

This experiment aimed to determine the best cellulose sources from the agricultural by product on the production of the thermostable cellulase enzymes by thermophilic bacteria (NG2) and also to find out the best concentration level of the selected sources of cellulose. The experiment was conducted in two stages using a completely randomized design (CRD). In phase 1, four cellulose sources were used, namely A: corn cobs, B: rice straw, C: coconut fiber, and D: oil palm trunk, in which the replication for each source was five. In phase 2,  the best sources of cellulose from the phase 1 was used at four different levels of concentration, i.e., A: 2%, B: 3%, C: 4%, and D: 5%, in which replication for each concentration was five. The result of phase 1 showed that the corn cobs was the best cellulose source  in producing cellulase enzyme. This was indicated by the activity of 0.329 U/ml, the enzyme protein of 0.0328 mg/ml, and the specific activity of 10.165 U/mg, which were significantly higher (P<0.01) than those of other cellulose sources. The results of phase 2 showed that the concentration of 4% (w/v) had the highest (P<0.01) specific activity of 44.002 U/mg  compared with those of the other concentrations. In conclusion, the highest production of cellulase enzyme using thermophilic bacteria (NG2) was obtained at the concentration of 4% (w/v) using the corn cobs as the cellulose source.
Isolasi Dan Karakteristik Fungi Lignoselulolitik Dari Limbah Sawit Sebagai Agen Pendegradasi Pakan Berserat Yunilas Yunilas; Lili Warly; Yetti Marlida; Irsan Ryanto
Rona Teknik Pertanian Vol 12, No 2 (2019): Volume 12, No. 2, Oktober 2019
Publisher : Department of Agricultural Engineering, Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (968.15 KB) | DOI: 10.17969/rtp.v12i2.10112

Abstract

Abstrak. Penelitian ini bertujuan untuk mengisolasi dan mengkarakteristik fungi lignoselulolitik dari limbah sawit sebagai pendegradasi serat (senyawa polisakarida). Penelitian ini menggunakan metode eksplorasi melalui isolasi, karakteristik, uji degradasi lignoselulosa dan identifikasi. Isolasi menggunakan medium selektif yang dimodifikasi mengandung carboxy methyl cellulose (CMC), xylan, lignin dan manan. Dari hasil isolasi diperoleh 16 isolat fungi lignoselulolitik dan 4 diantaranya memiliki kemampuan tinggi dalam mendegradasi lignoselulosa yaitu isolate fungi YLF2, YLF3, YLF4 dan YLF8. Isolat fungi yang diperoleh memiliki karakteristik yang bervariasi meliputi bentuk, permukaan, tepi dan warna koloni. Hasil uji degradasi (hidrolitik) menunjukkan bahwa isolat fungi YLF8 menghasilkan indeks hidrolitik lebih tinggi dibanding fungi lainnya. Berdasarkan hasil dapat disimpulkan bahwa isolat fungi YLF8 termasuk pada strain Trichoderma sp berpotensi sebagai isolat pendegradasi serat dan dapat digunakan sebagai bioktivator dalam fermentasi pakan berserat.Isolation And Characteristic Of Lignocellulolitic Fungi Of Palm Waste As a Fiber Feed Degrading AgentAbstract. This study aims to isolate and characterize lignocellulolytic fungi from palm wastes as fiber degradation (polysaccharide compounds). This research uses exploration method through isolation, characteristic, lignocellulosic degradation test and identification. Isolation using modified selective medium contains carboxy methyl cellulose (CMC), xylan, lignin and manan. From isolation result obtained 16 isolates of lignocellulolytic fungi and 4 of them have high ability in degrading lignocellulose that is fungi YLF2, YLF3, YLF4 and YLF8. The obtained fungi isolates have varying characteristics including shape, surface, edges and colony color. The result of degradation test (hydrolytic) showed that YLF8 fungi isolates yielded higher hydrolytic index than other fungi. Based on the results it can be concluded that the isolates of YLF8 fungi belong to the Trichoderma sp strain potentially as fiber degrading isolates and can be used as bioctivators in fibrous fermentation feed. 
SUPLEMENTASI BAGASE DENGAN ENZIM SELULASE DAN PENGARUHNYA TERHADAP KECERNAAN BAHAN KERING, BAHAN ORGANIK DAN SERAT KASAR SECARA IN VITRO Yetti Marlida; Mardiati Zain
Jurnal Riset Kimia Vol. 1 No. 1 (2007): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v1i1.98

Abstract

 ABSTRACTSupplementation of cellulase enzyme to agro-industrial residues can increase the nutritional value of the product. Advances in industrial biotechnology offer potential opportunities for economic utilization of agro-industrial residues such as palm pres fiber, rice straw, corn straw and bagase for animal feed. Bagase is a complex material, is the major by product of the sugar cane industry.  This experiment examined the effect of applying a cellulase enzyme (Siqma & Co) on the digestibility of bagase in vitro.  The enzyme was applied in liquid form at  concentrations 1.0; 1.5 and 2.0 (g/100 g DM) to bagase. The bagase was incubated with enzyme for 48 h before in vitro digestion. The digestibility of dry matter, organic matter and fiber  in vitro was detected. The results showed that digestibility of dry matter, organic matter and fiber were significantly different (P<0.01) across treatments. The increased of the cellulase enzyme supplemented to bagase can increased the nutritive values of the product and directly digestibility of dry matter, organic matter and fiber higher.  Results from this study indicated that direct application of enzymes to bagase was capable to improving  digestibility in-vitro. Key words :  cellulase enzyme, bagase, digestibility, in vitro
PRODUKSI GLUKOSA DARI BATANG KELAPA SAWIT MELALUI PROSES HIDROLISIS SECARA ENZIMATIS MENGGUNAKAN AMILASE TERMOSTABIL Yetti Marlida; Mirzah -; Syukri Arief; Khoirul Amru
Jurnal Riset Kimia Vol. 7 No. 2 (2014): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v7i2.190

Abstract

ABSTRACTThis study aimed to explore the optimization of the concentration of oil palm trunks andthermostable amylase enzyme preformance produce highest sugar (glucose total and reducingsugars) as well as the degree of polymerization. The design used in this study was a completelyrandomized design (CRD) factorial using 2 factors: factor A which consists of four levels ofamylase enzyme that A1: 250 units / kg, A2: 500 units / kg, A3: 750 units / kg and A4: 1000 units/ kg. Factor B is the concentration of oil palm trunk yitu B1: 20% (200 g / 1 liter) B2: 40% (400g / 1 liter) and B3: 60% (600 g / 1 liter) were repeated 3 times. The results showed that there is ahighly significant interaction (P <0.01) in the levels of amylase enzyme (factor A) with theconcentration of oil palm trunks (factor B) on reducing sugar, siqnificantly interaction (P <0.05)on total sugars and degree of polymerization (DP) . This research can be concluded that the besttreatment at a concentration of 60% oil palm trunks and levels 250 unit/kg of an enzymeconcentration that produces of total sugars 7.86 mg/ml, reducing sugar 15.69 mg /ml and degreeof polymerization 0.50Keywords : amilase, total gula, gula pereduksi, derajat polimerisasi.