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Efektivitas 3 spesies lebah madu sebagai agen polinasi untuk meningkatkan produktivitas (>40%) biji jarak pagar (Jatropha curcas) pada ekosistem iklim basah . Kasno; A.E. Zainal Hasan; Dedi Sholeh Efendi; . Syaefuddin
Jurnal Ilmu Pertanian Indonesia Vol. 15 No. 1 (2010): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1409.283 KB)

Abstract

The physic nut (Jatropha curcas) is one of potential plant for producing biodiesel, however efforts to socialization have still encounter constraints. The production system so far has not come to a level of attractive to the fellow farmers due to low productivity. Therefore further researches on production system are still urgently needed. This research entitled "The effectiveness of Three Species of Honey Bees as Pollinating Agent to Increase Seed Productivity" was carried out at Pakuwon, Sukabumi. The study site is a physic nut's seeds production center. The immediate objectives of the research was to identify the indirect impact of pollination of honey bees on physic nut farm on increasing its seed productivity while the further objective was to increase former's income. To achieve the immediate objective of the research, a set of experiment was conducted. Three species of honey bees namely Apis mellifera, Apis cerana and Trigona iridipenis were tested as pollinating agent to flowering physic nut plants under screen coverage. The size screen cover was 8 m long, 4 m wide and 2.5 m to cover 32 flowering physic nut plants. The treatment consisted of one and two colonies of honey bee species were released into each screened plot for 3 weeks. The untreated plot has no bee colony. The treatment was replicated 3 times. Complete Randomized Design procedure was followed in the experiment. Fruit sampling was done 50-55 days after colonies placement. Results of the first year experiment shows that honey bees as pollinating agent, especially A . cerana and A. mellifera provide significant positive indirect impact on seed productivity of physic nut plants. Assuming the market price of seed is not decreasing, such increase of seed productivity may also increase farmer's income from selling the harvested seeds.
Isolation and Characterization of Dextransucrase from Bacterial Isolate Sugar Cane A.E. Zainal Hasan; Laksmi Ambarsari; . Hasim; Aisyah Girindra
Indonesian Chemistry Letter Vol 1 No 1 (2001): Buletin Kimia
Publisher : Department of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (849.776 KB) | DOI: 10.29244/bk.1.1.1-6

Abstract

Dextransucrase is a glucasyltransferase that catalyzes the transfer of an alpha-D-glucopyranosyl group from sucrose to dextran and releasing the fructose. Enzyme activity was determined by measuring the initial reaction rate of fructose released from sucrose. A standard unit (U) is defined as the amount of enzyme that catalyzes the formation of 1 umol of D-fructose per mg of protein per minute. Dextransucrase was produced by fed-bath cultures of isolate bacterial from sugar cane in a mixture containing sucrose (10 % b/v) in themedium fermentation. Extracellular dextransucrase was obtained from bacterial isolate from sugar cane. Final culturedextransucrase activity was 0,2776 Ulmg of protein. Dextransucrase purified by ethanol 80 % showed activity of 4,2077 Ulmg of protein or activity increase 1515,75 %. Temperature optimum was 40 OC and pH optimum was 7.6. All of the metal increased the activity, except HgC12. Dectransucrase kinetics constants, Km is 3,00 mM and Vmaks is 3,5559 unit/mg of proteinlmin. Molecular weight is 95.155 kDa.
OPTIMIZATION CONDITION EXTRACTION USED ENZYME WITH RESPONSE SURFACE METHODOLOGY TO EXTRACT ROSELLE PETALS (Hibiscus sabdariffa L.) Mardiah Mardiah; Rima Nidaul Hasanah; Noli Novidahlia; A.E. Zainal Hasan
Jurnal Pertanian Vol. 9 No. 2 (2018): OKTOBER
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jp.v9i2.1477

Abstract

Roselle petals can be made into extracts as a source of raw material for food products and other products. Previous research states that the use of pectinase and cellulase enzymes can increase the yield including the anthocyanin content. This study aims to optimize the extraction conditions of roselle flower petals  (Hibiscus sabdariffa L.) using pectinase and cellulase enzymes by using Response Surface Methodology (RSM). The treatment conditions were using pectinase and cellulase enzyme (1: 1) with concentrations in the range between 500-1500 ppm, extraction temperature between 35-65 oC and extraction time between 30-90 minutes. Analysis of the best treatment was carried out on the yield level, anthocyanin content and total dissolved solids tested using central composite design, obtained R2 values for the three responses of 0.6. The results showed that based on the surface response, the optimum conditions of the best  extraction of the rosella at the addition of enzyme concentration of 1000 ppm, extraction temperature of 50 oC and extraction time of 10 minutes. In this condition, the yield of dried extracts of rosella was 84,20%, anthocyanins content 479,70 mg/L and total soluble solid 2,13 oBrix.