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All Journal International Journal of Evaluation and Research in Education (IJERE) HAYATI Journal of Biosciences Prosiding Seminar Biologi bionature Jurnal Kedokteran Brawijaya Proceeding International Conference on Global Resource Conservation The Journal of Pure and Applied Chemistry Research Proceeding of International Conference on Teacher Training and Education Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Scholaria: Jurnal Pendidikan dan Kebudayaan BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Penelitian Pendidikan IPA (JPPIPA) BIOEDUKASI Jurnal Mikologi Indonesia Bioedukasi: Jurnal Pendidikan Biologi Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Jurnal Pembelajaran dan Biologi Nukleus Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Sains & Matematika Journal of Biological Science and Education Bioscientist : Jurnal Ilmiah Biologi Proceeding Biology Education Conference Science Education and Learning Journal Journal of Biological Science and Education Abdinesia: Jurnal Pengabdian Kepada Masyarakat
Utami Sri Hastuti
Universitas Negeri Malang
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : bionature

 1 
Identifikasi dan Uji Kemampuan Hidrolisis pada Bakteri Amilolitik dan Proteolitik yang Diisolasi dari Wadi, Makanan Khas Kalimantan Tengah Choirunnisa, Hesti Nur; Sari, Ria Yustika; Hastuti, Utami Sri; Witjoro, Agung Witjoro
bionature Vol 18, No 2 (2017): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.895 KB) | DOI: 10.35580/bionature.v18i2.6138

Abstract

Abstract. Wadi is a sort of traditional foods that made of fish from Dayak tribe in Central Kalimantan. Wadi is made by the addition of salt and lumu. People make wadi as a supply of side dishes during difficult seasons of fish and stock for farming, hunting, or collecting forest products. In wadi, there are several bacteria species that capable in degrading amylum and protein. The bacteria capability in amylum and protein degradation can be determined by calculating the hydrolysis index. The aims of this study are to: (1) identify the amylolytic bacteria and proteolytic bacteria species from wadi, (2) determine the amylum and protein hydrolysis index on each amylolytic bacteria and proteolytic bacteria species isolated from wadi, and (3) determine the amylolytic bacteria and proteolytic bacteria species that have the highest hydrolysis index. This research is descriptive explorative research. The research results showed that: (1) there were four isolates of amylolytic bacteria isolated from wadi, i.e: Enterobacter agglomerans, Pseudomonas fluorescens, Acinetobacter baumannii, and Micrococcus varians, and there were five species of isolated proteolytic bacteria from wadi, i.e: Enterobacter agglomerans, Pseudomonas fluorescens, Nitrococcus mobilis, Acinetobacter baumannii, and Micrococcus varians, (2) the amylum hydrolysis index of Enterobacter agglomerans is 3.77; on Pseudomonas fluorescens is 3.00; on Acinetobacter baumannii is 4.16; and on Micrococcus varians is 7.23. The protein hydrolysis index of Enterobacter agglomerans is 3.22; on Pseudomonas fluorescens is 2.25; on Nitrococcus mobilis is 1.67; on Acinetobacter baumannii is 3.42; and on Micrococcus varians is 3.45, (3) Micrococcus varians has the highest amylum, i.e:  7.23 and protein hydrolysis index is 3.45.Keywords: hydrolysis index, amylolytic bacteria, proteolytic bacteria, wadi.
Identifikasi Uji Kemampuan Hidrolisis Lemak Dan Penentuan Indeks Zona Bening Asam Laktat Pada Bakteri Dalam Wadi Makanan Traditional Kalimantan Tengah Rizky, Mirza Yanuar; Fitri, Rizka Diah; Hastuti, Utami Sri; Prabaningtyas, Sitoresmi
bionature Vol 18, No 2 (2017): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.629 KB) | DOI: 10.35580/bionature.v18i2.6137

Abstract

Abstract. Wadi is a sort of fermented fish product from Dayak tribe in Central Kalimantan. Wadi is made with the addition of lumu, sugar, and salt, and fermented for 7-10 x 24 hours. During the fermentation process, there are microflora from fish, and microflora from lumu that play a role in fermentation process. Some species of bacteria that play a role in the wadi fermentation process are lipolytic bacteria and Lactic Acid Bacteria (LAB). This research aims to: (1) identify the species of lipolytic bacteria and LAB that present in the wadi, (2) determine the lipid hydrolysis index and lactic acid clearance zone index of lipolytic bacteria and LAB that present in the wadi, and (3) determine the species of lipolytic bacteria and LAB which have highest ability to hydrolyze lipid and have the highest ability to produce lactic acid based on lipid hydrolysis index and lactic acid clearance zone index. The results showed that, (1) there are four species of lipolytic bacteria and LAB present in wadi: Lactobacillus coryniformis, Lactobacillus casei, Nitrococcus mobilis and Streptococcus lactis; (2) the four species of bacteria have  different lipid hydrolysis index and lactic acid clearance zone index which are Lactobacillus coryniformis has a lipid hydrolysis index of 1.63 and lactic acid clearance zone index of 3.31; Lactobacillus casei has index of 1.94 and 3.96; Streptococcus lactis has index of 1.51 and 1.54 and Nitrococcus mobilis has index of 0.98 and 1.52; (3) Lactobacillus casei is a species of lipolytic bacteria and LAB which has the highest ability to hydrolyze lipid and to produce the highest lactic acid based on the lipid hydrolysis index and lactic acid clearance zone index, which are 1.94 and 3.96. Keywords: lipolitic bacteria, LAB, lipid hydrolysis index, lactic acid clearance zone, wadi.
Co-Authors Abdul Fattah Noor Abdul Ghofur Abdul Gofur Abdul Gofur Abdul Gofur Abdul Gofur Agung Witjoro Ahmad Najib Ajeng Daniarsih Ana Syarifatun Nisa Anggia Oktantia Anindya Nirmala Permata Arif Rahman Arini Zahrotun Nasichah Ary Maf’ula Aswal Salewangeng Aziz Tanama Balqis Balqis Betty Lukiati Chandra Kirana Chandra Kirana Choirunnisa, Hesti Nur Chomisatut Thoyibah Dina Istia’nah Dwi Listyorini Dwi Rahmawati Dwi Rahmawati Dwi Rakhmawati Dwi Rakhmawati Endang Suarsini Erna Wijayanti Faiza Nur Imawati Ningsih Fatchur Rohman Fatchur Rohman Febriani Sarwendah Asri Nugraheni Febriani Sarwendah Asri Nugraheni Fitri, Rizka Diah Fitria Maulita Fitria Maulita Galina Istiqhfarini Hadi Suwono Henny Nurul Khasanah Henny Nurul Khasanah Henny Nurul Khasanah Herawati Susilo Herawati Susilo Herdina Rahma Zalita Hesti Nur Choirunnisa Ibrohim Ibrohim Imam Mudakir Indah Sari Dewi Indriana Rahmawati Indriana Rahmawati Intan Rezki Kurniasari Istamar Syamsuri Izzalqurny, Tomy Rizky Izzatinnisa’ Izzatinnisa’ Khusnul Khotimah Lely Hermawati Linda Hapsari M. Ainur Yaqin M. Amin M. Nidhamul Maulana Mareta Arisswara Edy Mariyanti Mariyanti Mashuri Saputra Maya Firdausi Prayudhani Muhammad Andry Prio Utomo Muhammad Syamsussabri Nadila Sekar Zahida Natalia Rosa Keliat Ningsih, Husdiani Nur Laila Syafitri Nurul Yanuarsih Nuzalifa, Yossie Ulfa Otavia Dewi Permata Ika Hidayati Putri M. Al Asna Putri Moortiyani Al Asna Qorry Aulya Rohmana Rahel Natalia Saragih Munthe Rahmadyah Kusuma Putri Ria Yustika Sari Ria Yustika Sari Ria Yustika Sari Rina Kristina Maria Rizky, Mirza Yanuar Rofiqoh Lailatul Fitriyah Rozana, Kennis Safrudin M. Abidin Saidil Mursali Saidil Mursali Sari, Ria Yustika Siti Aisaroh Siti Annisaa'ul Kariimah Siti Hartina Pratiwi Siti Zubaidah Sitoresmi Prabaningtyas Sueb Sueb Sugeng Handiyanto Sugi Hartono Suhadi Suhadi Sulisetijono Umi Lestari Umu Fatonatul Hidayah W. F. Edi Hanzen W.F Edi Hanzen Wa Ode Nurhawa Yahmi Ira Setyaningrum Yessi Hermawati Yesy Maulina Nadhifah Yulia Venicreata Dipu Yulia Venicreata Dipu Yunita Putri Irsadul Ummah Yunita Rakhmawati